Abstract:
:We have reported the use of vectors based on avian leukosis virus (ALV) and ALV env pseudotyped lentivirus for stably introducing genes into somatic mammary cells in vivo for tumor modeling. These vectors require tva transgenic mice for viral infection. Here, we report, and described the method for, stably introducing genes into the mammary gland in mice using intraductal injection of a common lentiviral vector. This method does not need tva-transgenic mice for infection. This in vivo transformation method may be useful for rapid testing of the tumorigenic potential of genes potentially involved in breast carcinogenesis.
journal_name
J Mammary Gland Biol Neoplasiajournal_title
Journal of mammary gland biology and neoplasiaauthors
Bu W,Xin L,Toneff M,Li L,Li Ydoi
10.1007/s10911-009-9154-4subject
Has Abstractpub_date
2009-12-01 00:00:00pages
401-4issue
4eissn
1083-3021issn
1573-7039journal_volume
14pub_type
杂志文章abstract::Epithelial to mesenchymal transition (EMT) and its reversion via mesenchymal to epithelial transition (MET), represent a stepwise cycle of epithelial plasticity that allows for normal tissue remodelling and diversification during development. In particular, epithelial-mesenchymal plasticity is central to many aspects ...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-010-9180-2
更新日期:2010-06-01 00:00:00
abstract::It has long been appreciated that the estrogen receptor (ER) plays an important role in the biology of breast cancer. It is an accepted factor predicting favorable disease outcome and treatment response, and as such is generally considered to represent a "good" prognostic marker in breast cancer. In this review we pre...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1014782813943
更新日期:2001-10-01 00:00:00
abstract::Postpartum mammary gland involution is a mammalian tissue remodeling event that occurs after pregnancy and lactation to return the gland to the pre-pregnant state. This event is characterized by apoptosis and lysosomal-mediated cell death of the majority of the lactational mammary epithelium, followed by remodeling of...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-020-09451-6
更新日期:2020-06-01 00:00:00
abstract::The development and progression of aggressive breast cancer is characterized by genomic instability leading to multiple genetic defects, phenotypic diversity, chemoresistance, and poor outcome. Centrosome abnormalities have been implicated in the origin of chromosomal instability through the development of multipolar ...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/B:JOMG.0000048774.27697.30
更新日期:2004-07-01 00:00:00
abstract::Inflammation of the adipose tissues occurs in association with obesity. This inflammatory process leads to the induction of cyclooxygenase-2 (COX-2) expression and a consequent elevation in prostaglandin (PG) production, which, together with proinflammatory cytokines, induce aromatase expression and estrogen synthesis...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-013-9299-z
更新日期:2013-12-01 00:00:00
abstract::Nuclear Factor-kappaB (NF-kappaB) has been implicated in the lobuloalveolar development of the mammary gland. In breast cancer its activation has been linked to tumor progression via stimulation of cell proliferation, pro-survival, and angiogenesis pathways and metastasis. Whether NF-kappaB activation in the immune sy...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-006-9013-5
更新日期:2006-01-01 00:00:00
abstract::Various retroviral and lentiviral vectors have been used for up-the-teat intraductal injection to deliver markers, oncogenes, and other genes into mammary epithelial cells in mice. These methods along with the large number of genetically engineered mouse lines have greatly helped us learn normal breast development and...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章
doi:10.1007/s10911-020-09469-w
更新日期:2020-11-09 00:00:00
abstract::Originally adapted from the neurosphere assay, the nonadherent mammosphere assay has been utilized to assess early progenitor/stem cell frequency in a given population of mammary epithelial cells. This method has also been used to measure the frequency of tumorsphere initiating cells in both primary mammary tumors as ...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-012-9258-0
更新日期:2012-06-01 00:00:00
abstract::Estrogens are important for the development of the mammary gland and strongly associated with oncogenesis in this tissue. The biological effects of estrogens are mediated through the estrogen receptor (ER), a member of the nuclear receptor superfamily. The estrogen/ER signaling pathway plays a central role in mammary ...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1026339111278
更新日期:1997-10-01 00:00:00
abstract::During normal mammary gland development, the tyrosine kinase Jak2 and its main substrate, the signal transducer and activator of transcription-5 (Stat5), are critical for the growth and differentiation of alveolar progenitors as well as the survival of secretory mammary epithelial cells. Genetic studies in mouse model...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-008-9062-z
更新日期:2008-03-01 00:00:00
abstract::Colony stimulating factor 1 (CSF-1), a major regulator of the mononuclear phagocytic lineage, is expressed in more than 70% of human breast cancers and its expression is correlated with poor prognosis. Studies of CSF-1 null mutant mice demonstrated that CSF-1 plays an important role in normal mammary ductal developmen...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1020399802795
更新日期:2002-04-01 00:00:00
abstract::Mammary gland involution is a period of intensive tissue remodeling. Over the course of a relatively brief period, a large proportion of the mammary gland epithelium undergoes programmed cell death and is removed by phagocytes. In addition, the gland is cleared of residual milk fat globules as well as milk and adipocy...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-007-9036-6
更新日期:2007-03-01 00:00:00
abstract::Much of our knowledge about the intricate pathways and molecular mechanisms involved in the conversion of a normal mammary epithelial cell to malignancy derives from studies on mammary tumorigenesis induced by the retrovirus mouse mammary tumor virus. In addition, three DNA tumor viruses, simian virus 40, polyomavirus...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/BF02096302
更新日期:1996-01-01 00:00:00
abstract::The centrosome is the major microtubule organizing center of the cell and as such it plays an important role in cytoskeletal organization and in the formation of the bipolar mitotic spindle. Centrosome defects, characterized by abnormal size, number, and microtubule nucleation capacity, are distinguishing features of ...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1011312808421
更新日期:2001-04-01 00:00:00
abstract::The IGFBP family comprises six proteins with high affinity for the IGFs and several lower affinity IGFBP-related proteins. Their production in the breast is controlled by hormones, other local regulators and in tumors relates to the estrogen receptor status. Their functional activity can also be affected by various po...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1009519400591
更新日期:2000-01-01 00:00:00
abstract::The differentiation and function of mammary epithelial cells is dependent upon the combined action of growth factor/hormone receptors and integrin receptors, which act in concert to control the signals required for normal cell function. It is now becoming clear that integrin receptors also contribute to carcinoma cell...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1018766317055
更新日期:1999-10-01 00:00:00
abstract::The mouse has emerged as a primary animal model for human breast cancer because the mammary glands of the two species are very similar in structure and function. In this regard the TDLU and LA have similar morphology. The mouse, infected by MMTV, develops "spontaneous" tumors with specific but limited tumor phenotypes...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1018712905244
更新日期:1999-01-01 00:00:00
abstract::Analysis of genes and proteins involved in lipid biosynthesis in mammary epithelial cells (MECs) is complicated by the presence of adipose tissue in the mammary gland, which may be predominant in whole tissue lysates depending upon developmental stage. We have developed a method based on protocols used to establish pr...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章
doi:10.1007/s10911-009-9161-5
更新日期:2009-12-01 00:00:00
abstract::Available evidence indicates that mammary gland secretions first evolved in synapsids that laid parchment-shelled eggs. Unlike the rigid-shelled eggs of birds and some other sauropsids, parchment-shelled eggs lose water very rapidly when exposed to ambient air of lower vapor pressure, whether due to differences in rel...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1022848632125
更新日期:2002-07-01 00:00:00
abstract::The ErbB receptor tyrosine kinase family has often been associated with increased growth of breast epithelial cells, as well as malignant transformation and progression. In contrast, ErbB4/HER4 exhibits unique attributes from a two step proteolytic cleavage which releases an 80 kilodalton, nuclear localizing, tyrosine...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-008-9080-x
更新日期:2008-06-01 00:00:00
abstract::The relationship between maternal dietary intakes of energy or fat maternal body composition and the milk fat concentration is an important element in understanding the role of breast-milk in infant nutrition. In most studies in both developing and developed countries, no relation between maternal energy intake and mi...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章
doi:10.1023/a:1018702030259
更新日期:1999-07-01 00:00:00
abstract::The neuroendocrine hormone prolactin (PRL) stimulates breast growth and differentiation during puberty, pregnancy, and lactation. Despite extensive and convincing data indicating that PRL significantly contributes to the pathogenesis and progression of rodent mammary carcinoma, parallel observations for human breast c...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1026325630359
更新日期:1997-01-01 00:00:00
abstract::Premalignant breast disease in humans is a concept that admits to a broad range of elements and possible determinants predicting the likelihood of developing breast cancer. Most of these elements are relative, such as the risk of breast cancer for women that is 130 times that of men and peaks at a younger age by about...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1009521726605
更新日期:2000-10-01 00:00:00
abstract::Transgenic experiments using oocyte micro-injection methodology are often performed in order to target expression of a foreign gene in a specific tissue or, to a lesser extent, to study the regulation of gene expression. However, the isolation of embryonic stem cells in mice and the development of antisense and ribozy...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1018775729834
更新日期:1998-07-01 00:00:00
abstract::Milk is primarily regarded as a food furnishing essential nutrients for infant growth and development, but milk can also serve as a vehicle for mother to neonate transfer of molecules that regulate development. A wide array of biologically active compounds such as hormones, cytokines and enzymes are present in milk, e...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/BF02018079
更新日期:1996-07-01 00:00:00
abstract::The Notch receptor protein and its signaling pathway have been well conserved throughout evolution and appear to be pivotal components in cell fate decisions during development. Recent studies suggest that, depending on the cellular and developmental context, Notch signaling may also affect cell proliferation and prog...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1023/a:1009512414430
更新日期:2001-01-01 00:00:00
abstract::The microenvironment of a tumor has emerged recently as a critical contributor to the development of cancer. Within this environment, fibroblasts and immune cells are the cell lineages that seem to be active mediators of tumour development. The activated fibroblasts that are also present during wound healing and chron...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-014-9326-8
更新日期:2014-07-01 00:00:00
abstract::Mastitis is an inflammation of the mammary gland commonly caused by bacterial infection. The inflammatory process is a normal and necessary immunological response to invading pathogens. The purpose of host inflammatory responses is to eliminate the source of tissue injury, restore immune homeostasis, and return tissue...
journal_title:Journal of mammary gland biology and neoplasia
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doi:10.1007/s10911-011-9230-4
更新日期:2011-12-01 00:00:00
abstract::With improved screening methods, the numbers of abnormal breast lesions diagnosed in women have been increasing over time. However, it remains unclear whether these breast lesions will develop into invasive cancers. To more effectively predict the outcome of breast lesions and determine a more appropriate course of tr...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章
doi:10.1007/s10911-018-9407-1
更新日期:2018-12-01 00:00:00
abstract::As their name implies, the myoepithelial cells found at the epithelial-mesenchymal interface of the human mammary gland disclose features suggestive of a dual epithelial-like and muscle-like differentiation, i.e. they co-express various keratins and vimentin intermediate filaments, as well as smooth muscle-related ant...
journal_title:Journal of mammary gland biology and neoplasia
pub_type: 杂志文章,评审
doi:10.1007/s10911-005-9581-9
更新日期:2005-07-01 00:00:00