Abstract：
:The treatment of acute and chronic pain is still deficient. The modulation of glial cells may provide novel targets to treat pain. We hypothesize that astrocytes and microglia participate in the initiation and maintenance of both, acute surgical and chronic neuropathic pain. Rats underwent paw incision, L5 nerve exposure or L5 nerve transection surgery. Behavioral mechanical allodynia was assessed using von Frey filaments. Immunohistochemistry was performed using anti-ionized calcium binding adaptor protein, Iba-1 (microglia), and anti-Glial Fibrillary Acidic Protein, GFAP (astrocytes) on day 1, 4 and 7 after surgery. Following paw incision and at spinal L5 segment GFAP expression was increased in laminae I-II and Iba1 in deep laminae on day 1, in the entire dorsal horn on day 4 and dissipated on day 7 after paw incision in parallel with the allodynia. L5 nerve transection induced mechanical allodynia from day 1 to 7 which correlated with Iba-1 increases on day 1, 4 (entire dorsal horn) and day 7 after nerve injury (deep laminae of the dorsal horn) at spinal L5 segment. Conversely, GFAP increased at later time points from day 4 (deep laminae) and on day 7 (entire dorsal horn). Our data demonstrates that astrocytes (GFAP expression) play a role in the initiation of acute pain and the maintenance of chronic pain while Iba-1 increases closely correlated with the early phase of neuropathic pain. Iba1 and GFAP increased rostrally, at L3 segment, after paw incision (day 4) and only Iba1 increased following L5 nerve transection (day 7).
journal_name
Brain Resjournal_title
Brain researchauthors
Romero-Sandoval A,Chai N,Nutile-McMenemy N,Deleo JAdoi
10.1016/j.brainres.2008.05.004subject
Has Abstractpub_date
2008-07-11 00:00:00pages
116-26eissn
0006-8993issn
1872-6240pii
S0006-8993(08)01082-2journal_volume
1219pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Full limbic seizures were kindled in rats by repeated, bi-daily microinjections of glutamate (1.5 mumol) into the basolateral amygdala. Co-administration of the N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-7-phosphonoheptanoic acid with the glutamate, prevented the development of both electroencephalographi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91279-p
更新日期：1990-01-08 00:00:00
abstract：:Tyrosine hydroxylase immunohistochemical analysis was performed on tissue sections through the pallidal complex from Nemistrina monkeys which had been made hemi-parkinsonian by intracarotid MPTP infusion 8-12 months earlier. The side contralateral to the MPTP infusion showed a dense dopaminergic innervation of the pal...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90318-p
更新日期：1991-08-16 00:00:00
abstract：:Previous observations show that neonatal removal of nerve growth factor results in an increased number of fine dorsal root axons in the adult animal. The present study shows that the same treatment results in 49% more axons in the adult tract of Lissauer, which is an area of spinal white matter that contains predomina...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90262-4
更新日期：1988-10-04 00:00:00
abstract：:The density of specific prostaglandin E2 (PGE2) binding sites was quantitatively mapped in the rat brain using in vitro autoradiography. The anterior wall of the third ventricle and the nucleus solitary tract were found to have a very high density of binding sites (greater than 15 fmol/mg tissue). Two thalamic nuclei ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90720-t
更新日期：1992-05-29 00:00:00
abstract：:Preconditioning-induced cellular adaptation is a new therapeutic strategy for ischemic stroke. This research aims to examine the role of peroxisome proliferator activated receptor (PPAR)-γ co-activator 1-α (PGC-1α) and hypoxia induced factor-1α (HIF-1α) in hypoxic preconditioning-induced protection. In this study, rat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.01.059
更新日期：2012-04-04 00:00:00
abstract：:In scopolamine-treated (5.0 mg/kg, s.c.) rats hippocampal rhythmical slow activity (RSA) and neocortical low voltage fast activity (LVFA) occur only in close correlation with head movements, spontaneous changes in posture, or locomotion (Type I behavior). Previous work indicates that such scopolamine-resistant RSA and...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91500-e
更新日期：1992-11-27 00:00:00
abstract：:Choline uptake and ecto-cholinesterase activities have been measured in intact astroblast and neuroblast cultures. The data show that choline uptake is dependent upon the ionic composition of the culture medium and is sensitive to metabolic inhibitors. However, the high concentrations of the inhibitors necessary for t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90337-1
更新日期：1976-08-06 00:00:00
abstract：:A rabbit polyclonal antibody (91-01) was raised against recombinant human glia maturation factor beta (r-hGMF-beta). The antibody did not cross-react with a number of other growth factors on ELISA test. When compared with the monoclonal antibody G2-09 previously obtained, 91-01 immunoblotted the same protein band in r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90971-b
更新日期：1992-09-18 00:00:00
abstract：:The human neostriatum was found to contain large neurons (maximum diameter: 30-40 microns) that stain intensely for acetylcholinesterase (AChE). These neurons are few in number, representing less than 5% of the total striatal neuronal population, and appear uniformly scattered throughout the caudate nucleus and putame...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90663-2
更新日期：1984-01-16 00:00:00
abstract：:In extracellular recordings from chloral hydrate anesthetized rats the 5-HT3 antagonist, BRL 46470A, failed to prevent or reverse the increase in dopamine cell firing rate produced by systemic or iontophoretically applied morphine. A second 5-HT3 antagonist, tropesitron, was similarly found to be ineffective in antago...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90665-3
更新日期：1994-02-28 00:00:00
abstract：:The size differences in the midsagittal cerebellum, its individual lobules and its layers are studied in homozygous diabetic (HOM) and heterozygous Brattleboro rats on postnatal days 12, 24 and 180. As has been reported for total cerebellar weight, no significant reduction in cerebellar size in HOM rats occurs at day ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90296-8
更新日期：1986-05-01 00:00:00
abstract：:High levels of neurotensin/neuromedin N precursor mRNA, but few if any NT-positive perikarya have been detected in the dorsal subiculum of the adult rat or human hippocampus. This apparent discrepancy was tentatively ascribed to a lack of precursor mRNA translation or to a poor precursor posttranslational processing i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01083-3
更新日期：1995-12-08 00:00:00
abstract：:The spatio-temporal characteristics of release of acetylcholinesterase from the in vivo substantia nigra can now be described using a chemiluminescent reaction. Electrical stimulation of the striatum caused an increase in release of acetylcholinesterase within the ipsilateral substantia nigra; however, this increase i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90128-5
更新日期：1989-12-25 00:00:00
abstract：:The central nervous system (CNS) in adult mammals loses the ability to regenerate after injury. Although electrical stimulation (ES) has been shown to promote neural regeneration, the underlying mechanisms by which ES enhances CNS regeneration remain elusive. The aim of the present study was to investigate the effect ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.08.041
更新日期：2012-10-22 00:00:00
abstract：:Alzheimer's disease is a common age associated neurodegenerative disorder associated with an elevated risk of seizures that may be fundamentally connected to cognitive dysfunction. We used 4-9month-old mice of the APP/PS1 mouse model of Alzheimer's disease to study the presence of epileptiform-like discharges and to e...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.09.026
更新日期：2017-12-15 00:00:00
abstract：:Practical utilization of in vivo electrochemistry for brain studies has been impeded by difficulties with specificity and quantitative measurements. Studies in brain slices combined with similar data from intact animals have provided important new information on the nature of the electrochemical studies. Using the enz...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90901-0
更新日期：1983-10-24 00:00:00
abstract：:Dopamine-releasing protein (DARP) is a novel factor involved in the function and development of catecholaminergic systems. To test whether a peptide synthesized from the N-terminus of DARP (DARP-36aa) ameliorates deficits in nigrostriatal dopamine, rats were unilaterally lesioned with MPP+, 1 day before administration...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90291-7
更新日期：1994-12-12 00:00:00
abstract：:It has been demonstrated previously that kappa opioid receptor agonists, such as dynorphin, inhibit oxytocin secretion in the rat. To determine whether kappa agonists act directly on oxytocin-containing magnocellular neurons to inhibit hormone secretion, we utilized immunofluorescence to examine the cellular localizat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02154-0
更新日期：2001-04-13 00:00:00
abstract：:Nerve growth factor (NGF) treatment, given as a single 200 BU intraocular injection at the time of optic nerve transection, was found to significantly accelerate the retinal ganglion cell response to axotomy in the newt (Notophthalmus viridescens). In the control series the per cent of neurons in the retinal ganglion ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90327-5
更新日期：1979-08-03 00:00:00
abstract：:We previously reported that oral l-citrulline (l-Cit) administration antagonizes neuronal cell death in hippocampus following transient brain ischemia and that oral glutathione (GSH) administration prevents neuronal death through antioxidant activity. Here, we tested potential synergy of combined l-Cit and GSH adminis...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.03.014
更新日期：2017-05-15 00:00:00
abstract：:Immunohistochemical studies have suggested that corticotropin-releasing factor (CRF) was a transmitter of the olivocerebellar projection. We used in situ hybridization histochemistry with a 35S-labeled oligodeoxyribonucleotide probe for CRF mRNA to show that inferior olivary neurons of rats, baboons, and humans synthe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0169-328x(86)90010-0
更新日期：1986-11-01 00:00:00
abstract：:The inhibitory neurotransmitter γ-aminobutyric acid (GABA) is known to be fundamental to the neuronal processes underlying visual orientation and vibrotactile frequency and amplitude discrimination. Previous studies have demonstrated that performance on visual and vibrotactile psychophysics tasks is associated with in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.09.021
更新日期：2018-12-15 00:00:00
abstract：:The present study investigated whether the effects of central cholinergic stimulation on thermoregulation during exercise are modulated by arterial baroreceptors. Wistar rats were submitted to sinoaortic denervation (SAD) or sham denervation (SHAM) and then fitted with a chronic guide cannula into the lateral cerebral...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.09.110
更新日期：2010-12-17 00:00:00
abstract：:Enkephalin immunoreactive neurons within the nucleus tractus solitarius (NTS) were found to project to the parabrachial nucleus of the cat with the use of a combination of immunohistochemistry and retrograde transport of horseradish peroxidase. Double labelled neurons were located in the medial, parvocellular and comm...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91521-1
更新日期：1988-03-01 00:00:00
abstract：:There is much debate on the role of the superior colliculus (SC) in turning behaviour. In order to clarify this issue, unilateral kainate lesions were made by infusing 0.25 microgram of kainate at two different anterior planes (0.8 mm apart), in the lateral or in the medial aspects of the deep collicular layers (DLSC)...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90557-1
更新日期：1982-04-08 00:00:00
abstract：:Ionic currents induced by glutamate, kainate, quisqualate, and N-methyl-D-aspartate (NMDA) in cultured septal neurons were analyzed by fluctuations analysis. The power spectrum (PWS) of NMDA current fluctuations always fitted a single Lorentzian. PWSs of the other agonists fitted the sum of two Lorentzians; however, t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91120-1
更新日期：1988-05-10 00:00:00
abstract：:Growth cones integrate a remarkably complex concert of chemical cues to guide axons to their appropriate destinations. Recent work suggests that integrins contribute to axon guidance by interacting with a wide range of extracellular molecules including axon guidance molecules, by mechanisms that are not fully understo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.08.045
更新日期：2013-11-06 00:00:00
abstract：:Spinal cord patterns of metabolic activity in a model of neuropathic pain were assessed in unanesthetized rats by the [14C]-2-deoxyglucose (2-DG) technique. Rats used in this procedure had demonstrable thermal hyperalgesia ipsilateral to sciatic nerve ligation and ipsilateral hindpaws that were lifted in a guarded pos...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91468-g
更新日期：1991-11-15 00:00:00
abstract：:The purpose of this study was to determine whether treatment with high-dose human serum albumin (HSA) would offer protection in a model of high-grade transient forebrain ischemia. Twenty-six fasted Wistar rats underwent bilateral common carotid artery occlusion and severe hypotension (50 mmHg) for 10 min. The agent (2...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01952-6
更新日期：1999-10-16 00:00:00
abstract：:The effects of various nucleotides on the fast tetrodotoxin-sensitive (f-TTX-S) and the slow tetrodotoxin-resistant (s-TTX-R) sodium currents in rat dorsal root ganglion (DRG) neurons were investigated using the patch-clamp technique. Nucleoside triphosphates (NTPs; ATP, GTP, UTP and CTP) and nucleoside diphosphates (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.01.061
更新日期：2004-05-01 00:00:00
