Abstract：
:A rabbit polyclonal antibody (91-01) was raised against recombinant human glia maturation factor beta (r-hGMF-beta). The antibody did not cross-react with a number of other growth factors on ELISA test. When compared with the monoclonal antibody G2-09 previously obtained, 91-01 immunoblotted the same protein band in rat brain extract. However, unlike G2-09 which immunostained only astrocytes and Bergmann glia, 91-01 stained neurons as well. Many but not all neurons in the central and peripheral nervous system were positive for GMF-beta. The larger cell population stained by the polyclonal antibody was most likely due to its increased sensitivity, although other explanations are possible. The presence of GMF-beta-like immunoreactivity in both neurons and glia raises the possibility of a wider range of cell-cell interaction than was previously considered.
journal_name
Brain Resjournal_title
Brain researchauthors
Wang BR,Zaheer A,Lim Rdoi
10.1016/0006-8993(92)90971-bsubject
Has Abstractpub_date
1992-09-18 00:00:00pages
1-7issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(92)90971-Bjournal_volume
591pub_type
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