Abstract：
:The aim of this study was to assess the effect of Alzheimer's disease has on the functional integrity of several signal transduction proteins. The relative levels of the G-protein alpha subunits Gs alpha-L, Gs alpha-S, Gi alpha-2 and G(o) alpha were measured by western blotting and found to be unchanged in membranes prepared from Alzheimer-diseased frontal cortex or hippocampus compared to control brains. However the activity of the G-protein associated enzyme, high affinity GTPase, was found to be reduced in the frontal cortex (reduced by 25%) and by a similar magnitude in the hippocampus (reduced by 27%) of Alzheimer subjects. The same membrane preparations were also assayed for the activity of adenylate cyclase. Basal enzyme activity was not significantly altered in Alzheimer diseased hippocampus, but was markedly reduced (by 45%) in the frontal cortex. The ability of fluoride and aluminium ions to stimulate adenylate cyclase was not significantly changed in either brain region. This suggests that G-proteins, especially Gs, are still able to interact with this enzyme. These results indicate that although the presence of Alzheimer's disease does not significantly alter G-protein levels, changes have taken place in the overall activity of these proteins. However this alteration does not affect their ability to stimulate adenylate cyclase activity.
journal_name
Brain Resjournal_title
Brain researchauthors
Ross BM,McLaughlin M,Roberts M,Milligan G,McCulloch J,Knowler JTdoi
10.1016/0006-8993(93)90798-rsubject
Has Abstractpub_date
1993-09-17 00:00:00pages
35-42issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(93)90798-Rjournal_volume
622pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Morphologically distinct subsets of mesotelencephalic neurons were sought following retrograde transport of Fluoro-Gold from iontophoretic injections relatively restricted to the medial shell, core or rostral pole subterritories of the nucleus accumbens. The diameter and lengths of Fluoro-Gold immunolabeled dendrites ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00556-6
更新日期：1995-08-14 00:00:00
abstract：:Metabolic stress imposed by epileptic seizures can result in mitochondrial dysfunction, believed to act as positive feedback on epileptogenesis and seizure susceptibility. As the mechanism behind this positive feedback is unclear, the aim of the present study was to investigate the causal link between acute mitochondr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2020.147193
更新日期：2021-01-15 00:00:00
abstract：:The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography and [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding studies were performed using selective antagonists to M1 (pirenzepine),...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90415-r
更新日期：1991-05-31 00:00:00
abstract：:Heterotopic transplantations of brain vesicles of a quail embryo into a chick embryo were carried out in order to elucidate if CNS anlagen have plasticity in differentiation at the 7-10 somite stage. Quail cells are distinguished from chick cells due to the difference in nuclear morphology. The prosencephalon did not ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90231-y
更新日期：1990-03-12 00:00:00
abstract：:Chemotherapy-induced pain is the most common treatment-limiting complication encountered by cancer patients receiving taxane-, vinca alkaloid- or platin-based chemotherapy. Several lines of evidence indicate that activation of pro-inflammatory cascades involving the release of cytokines including tumor necrosis factor...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.07.001
更新日期：2008-09-10 00:00:00
abstract：:Drugs thought to inhibit the actions of protein kinase C (PKC) and cAMP dependent protein kinase (A-kinase) were infused intrathecally into the subarachnoid space of the lumbar region of the spinal cord, and the effects on acoustic startle were measured. Previous work has shown that intrathecal infusion of drugs thoug...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90864-8
更新日期：1990-08-20 00:00:00
abstract：:The triggering of ectopic action potentials (APs) at axon terminals in a chronic epileptic cobalt focus was investigated in thalamocortical (TC) neurons of rats under urethane anesthesia. TC cells which were in register with an active epileptic aggregate discharged bursts of 2-11 APs. According to the rules of the col...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90628-m
更新日期：1992-03-27 00:00:00
abstract：:Light microscopic double labeling immunocytochemistry for vasoactive intestinal peptide (VIP) and gonadotrophin-releasing hormone (GnRH) was carried out on the hypothalami of male and female rats. It was found that the number of VIP boutons that terminate on GnRH neurons and the percentage of GnRH neurons contacted by...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00208-x
更新日期：1998-06-08 00:00:00
abstract：:Neonatal MK-801 treatment from postnatal day 8-19 leads to long-term effects on brain function, suggesting that exposure to this drug leads to the development of a brain with immature network properties. One aspect of this hypothesis, that the NMDA receptors preserve their immature state after the treatment, has been ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91152-5
更新日期：1994-11-28 00:00:00
abstract：:These experiments were designed to test the hypothesis that the TxA2 mimetic, U46,619, would stimulate group III and IV afferent nerve endings from the hindlimb of the anesthetized cat. Nerve impulses were recorded from the dorsal rootlets of the L7-S1 segments of the spinal cord, and afferent units identified by meas...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01211-5
更新日期：1997-01-02 00:00:00
abstract：:In order to better understand the neuroleptic-like effects of neurotensin in vivo, the effects of neurotensin in vitro on dopamine D2 and D1 agonist and antagonist binding sites were characterized in membranes from the neostriatum and the subcortical limbic area. Neurotensin increased the KD but not the Bmax value of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90753-i
更新日期：1991-10-04 00:00:00
abstract：:Suction lesions of the right frontal cerebral cortex in rats induce a period of spontaneous hyperactivity. The hyperactivity, as measured by an increase in running wheel activity begins about one week post-operatively and continues throughout the remainder of a 30-day observation period. The increased activity is acco...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91303-2
更新日期：1981-08-10 00:00:00
abstract：:Physical exercise can induce brain plasticity and reduce the cognitive decline observed in type 1 diabetes mellitus (T1DM). We investigated the effects of physical exercise to prevent or reverse spatial memory deficits produced by diabetes and some biochemical and immunohistochemical changes in hippocampal astrocytes ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.10.024
更新日期：2017-01-15 00:00:00
abstract：:Experimental thiamine deficiency (TD) is a model of impaired oxidative metabolism associated with region-selective neuronal loss in the brain. Oxidative stress is a prominent feature of TD neuropathology, as evidenced by the accumulation of heme oxygenase-1 (HO-1), ferritin, reactive iron and superoxide dismutase in m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02933-4
更新日期：2000-12-01 00:00:00
abstract：:C57BL/6J (B6) and DBA/2J (D2) mice have been characterized previously as seizure-resistant and seizure-sensitive, respectively, a distinction based primarily upon a differential response to the convulsant effects of various drugs. In the present study, electroconvulsive shock (ECS) was used to assess maximal electrosh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)01013-0
更新日期：1998-11-30 00:00:00
abstract：:Dissociated 4-day (stage 23) chick embryo lumbar cord cells were cultured at low or high cell densities for 1 or 5 days in the presence or absence of added spinal neuronotrophic factor (supplied as RN22 Schwannoma conditioned medium, RCM). In low density, 1-day cultures neuronal survival was dependent on added RCM whe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91039-9
更新日期：1983-05-09 00:00:00
abstract：:The central nucleus of the inferior colliculus (IC) is a laminated structure that receives multiple converging afferent projections. These projections terminate in a layered arrangement and are aligned with dendritic arbors of the predominant disc-shaped neurons, forming fibrodendritic laminae. Within this structural ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.07.055
更新日期：2007-10-10 00:00:00
abstract：:The origin of neuronal perikarya which project to the external zone of the median eminence (the tuberoinfundibular neuronal system) was determined in the rat after injection or diffusion of wheat germ agglutinin (WGA) into the median eminence. The retrogradely transported lectin was detected in neurons using an immuno...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90334-1
更新日期：1982-08-05 00:00:00
abstract：:Immunohistochemistry for Kv1.2, a subunit of voltage-gated K(+) channels, was performed on the trigeminal ganglion (TG). Immunoreactivity (ir) was detected in half (48%) the TG neurons. These neurons were mostly medium-sized to large (range 137.6-2664.8 microm(2), mean+/-S.D. 892.6+/-413.3 microm(2)). A double immunof...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02583-6
更新日期：2003-06-06 00:00:00
abstract：:Placental Opioid-Enhancing Factor (POEF) is a substance found in amniotic fluid (AF) that, when ingested, potentiates opioid-mediated, but not non-opioid-mediated, hypoalgesia. Vaginal-cervical stimulation (VCS) produces a stimulus-bound, partially opioid-mediated hypoalgesia that previous research has shown to be pot...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.12.055
更新日期：2011-02-28 00:00:00
abstract：:The effect of L-AP4, a group III mGluR agonist, on sensory synaptic transmission in the lamprey spinal cord has been analyzed. Paired recordings were made between cutaneous mechanosensory neurons (dorsal cells) and postsynaptic spinobulbar giant interneurons. L-AP4 reduced the monosynaptic dorsal cell-evoked EPSP, but...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02462-9
更新日期：2002-05-24 00:00:00
abstract：:Male guinea pigs were given 3 tests for sexual behavior. Animals that never ejaculated were classified as low activity (LA), animals that ejaculated on one test were classified as medium activity (MA), and animals that ejaculated on two or more tests were classified as high activity (HA). Subsequently, animals from ea...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90929-x
更新日期：1976-03-19 00:00:00
abstract：:It has been demonstrated that repeated, subcutaneous administration of 3,4-methylenedioxymethamphetamine (MDMA) to rats, guinea pigs, and squirrel monkeys produces long-lasting depletions of serotonin (5-hydroxytryptamine; 5-HT) in several brain regions. Since evidence of degenerating 5-HT neurons has been observed in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90700-2
更新日期：1989-05-29 00:00:00
abstract：:One monoclonal antibody 115A10 (MAb 5A10) specifically stained a subpopulation of retinal bipolar cells in various vertebrates. In the bullfrog retina, MAb 5A10 stained the large bipolar cells, but not the small bipolar cells. Labeling of the living bipolar cells was observed in isolated cell preparation of the frog r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90919-x
更新日期：1987-07-28 00:00:00
abstract：:High levels of neurotensin/neuromedin N precursor mRNA, but few if any NT-positive perikarya have been detected in the dorsal subiculum of the adult rat or human hippocampus. This apparent discrepancy was tentatively ascribed to a lack of precursor mRNA translation or to a poor precursor posttranslational processing i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01083-3
更新日期：1995-12-08 00:00:00
abstract：:An experiment was performed in order to determine the role of the amygdala and surrounding cortex in quiet biting attack elicited from electrical stimulation of the hypothalamus. Stimulation of basal, cortical, and anterior amygdala as well as pyriform cortex and parahippocampal gyrus resulted in a suppression of the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90433-3
更新日期：1980-09-15 00:00:00
abstract：:Met-enkephalin-like immunoreactivity in microdialysates from the respiratory-related nucleus tractus solitarii was determined simultaneously with ventilatory responses in seven, spontaneously breathing, developing swine under conditions of normoxia, hypoxia and recovery from hypoxia for 30 min each. Assayed levels of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00541-w
更新日期：1995-07-31 00:00:00
abstract：:Chronic neuropathic pain has demonstrated that coexisting psychiatric disorders are associated with disability and poorer treatment outcomes. Hyperpolarization-activated cyclic nucleotide-gated (HCN, Ih) channels play a major role in pain via hyperexcitability and facilitation of ectopic firing in neurons. Neuronal hy...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2019.03.036
更新日期：2019-08-15 00:00:00
abstract：:Parkinson's disease (PD) is a common neurodegenerative disease characterized by progressive loss of midbrain dopaminergic neurons. To gain an insight into the mechanisms underlying the progression of PD, gene expression analysis was performed using two different brain regions, the substantia nigra pars compacta (SN) a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.05.066
更新日期：2010-07-30 00:00:00
abstract：:The intracerebroventricular injection (1 microgram) of murine corticotropin-releasing factor (r-CRF) to rats after 72 h of sleep deprivation markedly postponed the sleep onset, reduced slow wave sleep and prolonged the duration of paradoxical sleep (PS) episodes. Moreover, CRF is-to our knowledge-the first compound to...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90614-h
更新日期：1990-05-07 00:00:00
