Diagnosing platelet delta-storage pool disease in children by flow cytometry.

Abstract:

:Bleeding problems are symptomatic of platelet delta-storage pool diseases (SPDs) such as Hermansky-Pudlak syndrome. Although at present no cure is available for delta-SPD, early diagnosis is of great importance for prophylactic and supportive treatment. This study tested the usefulness of a flow cytometric assay for platelet serotonin in children. The assay was used to diagnose delta-SPD in a 10-year-old girl. Platelet serotonin levels were significantly lower in the patient than in all healthy control subjects (10 children and 10 adults). The serotonin results were supported by traditional tests, which are transmission electron microscopy of whole mounts and adenosine triphosphate release by lumi-aggregometry. The flow cytometric serotonin assay is a major improvement to current pediatric diagnostics. The advantages of this test are small sample volume of fresh or fixed/frozen platelets, availability of objective results within 2 hours of obtaining the blood sample, and automated analysis by flow cytometry.

journal_name

Am J Clin Pathol

authors

Maurer-Spurej E,Pittendreigh C,Wu JK

doi

10.1309/3KRYCPNAPDTVFWGY

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

626-32

issue

4

eissn

0002-9173

issn

1943-7722

pii

G7WW6R7X06643455

journal_volume

127

pub_type

杂志文章
  • B-acute lymphoblastic leukemia/lymphoblastic lymphoma.

    abstract:OBJECTIVES:This session of the 2013 Society of Hematopathology/European Association for Haematopathology Workshop was dedicated to B-acute lymphoblastic leukemia (B-ALL)/lymphoblastic lymphoma (LBL) with recurrent translocations and not otherwise specified. METHODS:In this review, we summarize the cases discussed duri...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/AJCPAN7BH5DNYWZB

    authors: Loghavi S,Kutok JL,Jorgensen JL

    更新日期:2015-09-01 00:00:00

  • Diagnostic yield from 231 autopsies in a community hospital.

    abstract::Autopsy reports of 231 adult autopsies were examined for clinically unexpected diagnoses. Analysis of data showed that 97 of 188 autopsy diagnoses were clinically unexpected. The diagnosis of pulmonary embolism was clinically the most frequently missed diagnosis relative to its actual occurrence. Also noted was that t...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/93.4.486

    authors: Rao MG,Rangwala AF

    更新日期:1990-04-01 00:00:00

  • The New Equivocal: Changes to HER2 FISH Results When Applying the 2013 ASCO/CAP Guidelines.

    abstract:OBJECTIVES:Human epidermal growth factor receptor 2 (HER2, ERBB2) testing is an important prognostic/predictive marker in breast cancer management, especially in selecting HER2-targeted treatment. American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines address HER2 status and were...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP3Q9WFOQTKUVV

    authors: Long TH,Lawce H,Durum C,Moore SR,Olson SB,Gatter K,Troxell ML

    更新日期:2015-08-01 00:00:00

  • Evaluation of three commercial enzyme immunoassays for toxoplasma and cytomegalovirus antibodies.

    abstract::Three commercially available enzyme immunoassays (EIAs) were evaluated for specificity and sensitivity compared to immunofluorescent assays (IFAs) for detection of IgG and IgM antibodies to Toxoplasma gondii (Toxo) and Cytomegalovirus (CMV). A panel of 78 sera were tested, including specimens known to contain Toxo IgG...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.3.428

    authors: Van Enk RA,James KK,Thompson KD

    更新日期:1991-03-01 00:00:00

  • T-cell lymphoma after heart transplantation.

    abstract::A 26-year-old woman who had undergone orthotopic heart transplantation because of dilative cardiomyopathy received a triple-drug immunosuppressive regimen (cyclosporine A, azathioprine, and prednisolone). During her relatively frequent episodes of acute rejection, she was treated with methylprednisolone and repeated a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.1.95

    authors: Kemnitz J,Cremer J,Gebel M,Uysal A,Haverich A,Georgii A

    更新日期:1990-07-01 00:00:00

  • A rapid paper-disc test for penicillinase.

    abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.557

    authors: Tu KK,Jorgensen JH,Stratton CW

    更新日期:1981-04-01 00:00:00

  • Meningorectal fistula as a cause of polymicrobial anaerobic meningitis.

    abstract::A 49-year-old diabetic woman developed polymicrobial anaerobic meningitis secondary to a meningorectal fistula one year after receiving neutron beam therapy for a rectal adenocarcinoma. The meningitis was refractory to chloramphenicol and penicillin but responded to oral metronidazole. Sustained eradication of meningi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.1.127

    authors: Walsh TJ,Weinstein RA,Malinoff H,Breyer MD,Berelowitz BA

    更新日期:1982-07-01 00:00:00

  • Detection of hepatitis C virus RNA sequences in B-cell non-Hodgkin lymphoma.

    abstract::Serologic testing shows that hepatitis C virus (HCV) may have a role in the pathogenesis of B-cell non-Hodgkin lymphomas (B-cell NHLs). We tried to demonstrate HCV RNA sequences in paraffin-embedded tissue from B-cell NHLs by reverse-transcription double polymerase chain reaction (RT-PCR) and Southern blotting. We stu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/REV9-FDTM-5NGC-HBWY

    authors: Karavattathayyil SJ,Kalkeri G,Liu HJ,Gaglio P,Garry RF,Krause JR,Dash S

    更新日期:2000-03-01 00:00:00

  • Method precision and frequent causes of errors observed in point-of-care glucose testing: a proficiency testing program perspective.

    abstract:OBJECTIVES:Method imprecision, error rates, and explanatory causes that were identified in the Institute for Quality Management in Healthcare point-of-care (POC) glucose proficiency testing (PT) program were assessed in comparison with results obtained from laboratory glucose PT surveys. METHODS:POC and laboratory glu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPP5YS2MVSKBYY

    authors: Aslan B,Stemp J,Yip P,Gun-Munro J

    更新日期:2014-12-01 00:00:00

  • Pathology consultation on the laboratory evaluation of thrombophilia: when, how, and why.

    abstract::Venous thromboembolism (VTE) results from the interaction of the Virchow triad (venous stasis, endothelial injury, and hypercoagulability). Risk factors for increased hypercoagulability, or thrombophilia, include activated protein C resistance/factor V Leiden, the prothrombin G20210A mutation, deficiencies of the natu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP5SQT3ZKYQFBM

    authors: Ballard RB,Marques MB,Education Committee of the Academy of Clinical Laboratory Physicians and Scientists.

    更新日期:2012-04-01 00:00:00

  • Comparison of Affirm VPIII and Papanicolaou tests in the detection of infectious vaginitis.

    abstract::To compare the Affirm VPIII molecular test (Becton Dickinson, Burlington, NC) with morphologic identification used in routine Papanicolaou (Pap) test screening in the detection and identification of Candida species, Trichomonas vaginalis, and Gardnerella vaginalis, we identified 431 cases with a concomitant Pap test a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP7TBN5VZUGLZU

    authors: Levi AW,Harigopal M,Hui P,Schofield K,Chhieng DC

    更新日期:2011-03-01 00:00:00

  • Variation in human blood high-density lipoprotein response to oral vitamin E megadosage.

    abstract::In a preliminary attempt to distinguish random intrinsic and methodologic variations of blood lipid levels from any that have possibly been induced by ingestion of vitamin E, blood samples were analyzed in duplicate for lipids several times before and during oral vitamin E administration. Three of eight subjects showe...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.2.165

    authors: Muckle TJ,Nazir DJ

    更新日期:1989-02-01 00:00:00

  • The Prognosis of Stage IA Mixed Endometrial Carcinoma.

    abstract:OBJECTIVES:To explore the survival and definition of stage IA mixed endometrial carcinoma. METHODS:From June 1, 2010, to June 1, 2017, cases with stage IA endometrial cancer were included in this study. The survival outcomes were compared among patients with endometrioid (group A), nonendometrioid (group B), and mixed...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqz083

    authors: Li W,Li L,Wu M,Lang J,Bi Y

    更新日期:2019-10-07 00:00:00

  • Performances of urine pregnancy tests on College of American Pathologists Survey specimens.

    abstract::The participant performances on the College of American Pathologists (CAP) Urine Survey specimens distributed during 1979 and 1980 for pregnancy testing are reviewed. Almost all the reagent methods used by the participants performed well on the negative specimens. Prominent differences in results on the positive speci...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Biesecker JL,Rippey JH

    更新日期:1981-10-01 00:00:00

  • AIDS-related lymphoma in Brazil. Histopathology, immunophenotype, and association with Epstein-Barr virus.

    abstract::The occurrence of malignant lymphoma is an increasingly important cause of morbidity and mortality in AIDS patients. The incidence of AIDS-related lymphoma in some developing countries such as Brazil is increasing as the survival of HIV infection has improved. Although there is a clear association between several type...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/105.2.230

    authors: Bacchi CE,Bacchi MM,Rabenhorst SH,Soares FA,Fonseca LE Jr,Barbosa HS,Weiss LM,Gown AM

    更新日期:1996-02-01 00:00:00

  • Hepatitis B (surface) antigen testing by radioimmunoassaymexperience in a very large volunteer donor population.

    abstract::The results of hepatitis B surface antigen (HBs-Ag) testing in a large volunteer blood donor population are described. Counterelectrophoresis and three versions of solid-phase radioimmunoassay technic are compared and evaluated. Initial results suggested that the radioimmunoassay technic are compared and evaluated. In...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/63.6.847

    authors: Dodd RY,Ni LY,Mallin WS,Greenwalt TJ

    更新日期:1975-06-01 00:00:00

  • Globin chain synthesis in Hb J Baltimore-beta (+)-thalassemia.

    abstract::An American black family in whom hemoglobin J Baltimore and beta (+)-thalassemia genes coexisted is described. The proposita is a 23-year-old woman with a hemoglobin (Hb) level of 11.5 g/dl, microcytic, hypochromic indices, increased values of Hbs A2 and F, and alpha/non-alpha synthetic ratio of 1.52. Hbs A and J Balt...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.6.843

    authors: Ballas SK,Atwater J,Theriault C,Kim HC,Propst M

    更新日期:1981-06-01 00:00:00

  • Immunochemical measurement of serum prostatic acid phosphatase (PAP). Clinical evaluation of radioimmunoassay and counter immunoelectrophoresis.

    abstract::Two radioimmunoassay procedures (RIA-1 and RIA-2) were evaluated for the quantitation of prostatic acid phosphatase in serum and compared with the enzymatic method and counter immunoelectrophoresis method for their specificity and sensitivity. Sera from 168 patients were analyzed and these included: normals, 27; untre...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/76.4.430

    authors: Gupta MK,Rajaraman S,Gupta SK,Hewitt CB,Deodhar SD

    更新日期:1981-10-01 00:00:00

  • erb-b2 amplification by fluorescence in situ hybridization in breast cancer specimens read as 2+ in immunohistochemical analysis.

    abstract::We conducted this study to ascertain the prevalence of erb-b2 gene amplification in breast cancer specimens read as 2+ in immunohistochemical analysis. Slides from patients with metastatic or recurrent breast cancer were eligible for fluorescent in situ hybridization (FISH) study if they were read as 2+ immunohistoche...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/R2X4KK22QCL7PLME

    authors: Lan C,Liu JM,Liu TW,Hsu DH,Liang S,Chen JR,Peng JW

    更新日期:2005-07-01 00:00:00

  • A collaborative approach to lean laboratory workstation design reduces wasted technologist travel.

    abstract::Lean methodologies have been applied in many industries to reduce waste. We applied Lean techniques to redesign laboratory workstations with the aim of reducing the number of times employees must leave their workstations to complete their tasks. At baseline in 68 workflows (aggregates or sequence of process steps) stu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPE0PI2ENWYWMU

    authors: Yerian LM,Seestadt JA,Gomez ER,Marchant KK

    更新日期:2012-08-01 00:00:00

  • Immune hemolytic anemia associated with probenecid.

    abstract::Upon hospital admission a patient was found to have severe anemia and a strongly positive direct antiglobulin test (DAT). The patient was taking probenecid periodically for gout. An antibody was detected in the patient's serum that only reacted with red blood cells (RBCs) when probenecid was added. Eluates from the pa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.3.391

    authors: Sosler SD,Behzad O,Garratty G,Lee CL,Postoway N,Khomo O

    更新日期:1985-09-01 00:00:00

  • Hepatic oncocytes. Incidence, staining characteristics, and ultrastructural features.

    abstract::Hepatocytes with orcein-positive ground-glass cytoplasm have been shown to contain abundant hepatitis B surface antigen in the cisternae of excess smooth endoplasmic reticulum. Hepatocytes with similar cytoplasmic changes that did not react with orcein were observed in 26.5% of 49 cases of cirrhosis. These cells exhib...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.498

    authors: Gerber MA,Thung SN

    更新日期:1981-04-01 00:00:00

  • Liquid-based Papanicolaou tests in endometrial carcinoma diagnosis. Performance, error root cause analysis, and quality improvement.

    abstract::Recent reports show that the sensitivity of endometrial carcinoma detection on liquid-based Papanicolaou (Pap) tests (88%) is considerably higher than that reported on conventional Pap smears (20%-30%), although few laboratories have corroborated these results. We performed a 5-year retrospective review of all liquid-...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPLFBK1A2XJDQI

    authors: Sams SB,Currens HS,Raab SS

    更新日期:2012-02-01 00:00:00

  • Evaluation of HPV-16 and HPV-18 genotyping for the triage of women with high-risk HPV+ cytology-negative results.

    abstract::The ATHENA (Addressing THE Need for Advanced HPV Diagnostics) HPV study evaluated the clinical usefulness of the cobas HPV Test (Roche Molecular Systems, Pleasanton, CA) for high-risk human papillomavirus (HR-HPV) testing (14 HR types) and individual HPV-16/HPV-18 genotyping in women undergoing routine cervical cytolo...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章

    doi:10.1309/AJCPTUS5EXAS6DKZ

    authors: Wright TC Jr,Stoler MH,Sharma A,Zhang G,Behrens C,Wright TL,ATHENA (Addressing THE Need for Advanced HPV Diagnostics) Study Group.

    更新日期:2011-10-01 00:00:00

  • Diagnostic yield of transbronchoscopic biopsies.

    abstract::Transbronchoscopic biopsies of lung (transbronchial) or bronchus (endobronchial) have a high diagnostic of yield when performed by a single expert bronchoscopist or a small group of expert bronchoscopists. This procedure's diagnostic yield was evaluated in a general hospital where biopsies are performed by a diverse g...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/72.6.926

    authors: Jenkins R,Myerowitz RL,Kavic T,Slasky S

    更新日期:1979-12-01 00:00:00

  • Gastrointestinal microvillus inclusion disease.

    abstract::A 3-year-old girl of Navajo heritage had intractable diarrhea beginning at 4 days of age and resulting in long-term hyperalimentation. Investigation before multivisceral transplantation included biopsies of the rectum, stomach, duodenum, and liver. The diagnosis of microvillus inclusion disease was established by docu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/98.1.119

    authors: Schofield DE,Agostini RM Jr,Yunis EJ

    更新日期:1992-07-01 00:00:00

  • Analysis of bacterial detection in whole blood-derived platelets by quantitative glucose testing at a university medical center.

    abstract::After the March 2004 implementation of American Association of Blood Banks standards regarding platelet bacterial detection, we began quantitative glucose screening of whole blood-derived platelets (WB-P). The glucose level was measured immediately before component release--often storage day 4 or 5--using the Glucomet...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPVN9OT4GRCSKO

    authors: McKane AV,Ward N,Senn C,Eubanks J,Wessels L,Bowman R

    更新日期:2009-04-01 00:00:00

  • Fine-needle cytology of 292 palpable orbital and eyelid tumors.

    abstract::Fine-needle cytology (FNC) of 292 palpable orbital and eyelid tumors was performed with a 25-gauge (0.5-mm), 3-cm needle and compared with the histopathologic findings in 286 cases. Among these 286 cases, a concordant diagnosis of malignancy and type was achieved in 249 cases (87%). False positive diagnoses were made ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/93.1.100

    authors: Zajdela A,Vielh P,Schlienger P,Haye C

    更新日期:1990-01-01 00:00:00

  • Serum concentrations of allergen-specific IgG antibodies in inhalant allergy: effect of specific immunotherapy.

    abstract::The occurrence of antibodies belonging to IgG class of immunoglobulins with specificity for short ragweed and Bermuda grass in the sera of nonatopic subjects and patients with inhalant allergy (at the time of initial diagnosis, following specific immunotherapy was investigated with an enzyme immunoassay. The intra-ass...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.3.290

    authors: Ali M,Ramanarayanan MP,Nalebuff DJ,Fadal RG,Willoughby JW

    更新日期:1983-09-01 00:00:00

  • Determination of urinary homovanillic acid using the nitrosonaphthol reaction.

    abstract::Determination of urinary homovanillic acid (HVA) is an important analysis to establish the diagnosis and prognosis of neuroblastoma. A method is presented for the determination of this metabolite of dopamine using its reaction with 1-nitroso-2-naphthol and nitrous acid. The method has shown a sensitivity, specificity,...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/87.5.644

    authors: Rothstein A

    更新日期:1987-05-01 00:00:00