Detection of hepatitis C virus RNA sequences in B-cell non-Hodgkin lymphoma.

Abstract:

:Serologic testing shows that hepatitis C virus (HCV) may have a role in the pathogenesis of B-cell non-Hodgkin lymphomas (B-cell NHLs). We tried to demonstrate HCV RNA sequences in paraffin-embedded tissue from B-cell NHLs by reverse-transcription double polymerase chain reaction (RT-PCR) and Southern blotting. We studied 31 consecutive cases of B-cell NHLs; lymph nodes from 32 patients with diseases other than B-cell NHL were negative controls. Positive-strand HCV RNA was tested with primers for the 5' untranslated region. Replicative negative strand HCV RNA was tested with strand-specific RT-PCR for the 5' untranslated region. Immunohistochemical staining for HCV was done using an antibody to HCV core protein. Positive-strand HCV RNA was detected in 8 patients with B-cell NHL; negative-strand HCV RNA was detected in 6 of these cases, indicating viral replication. All control cases were negative for HCV RNA. Immunohistochemistry showed no staining of lymphoma cells for HCV core proteins in any case. HCV and B-cell NHLs may be associated. RT-PCR on paraffin-embedded lymphoma tissue is an alternative method of testing for HCV. The value of immunohistochemistry could not be ascertained. The exact role of HCV in the pathogenesis of B-cell NHL needs to be studied further.

journal_name

Am J Clin Pathol

authors

Karavattathayyil SJ,Kalkeri G,Liu HJ,Gaglio P,Garry RF,Krause JR,Dash S

doi

10.1309/REV9-FDTM-5NGC-HBWY

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

391-8

issue

3

eissn

0002-9173

issn

1943-7722

journal_volume

113

pub_type

杂志文章
  • Analysis of bacterial detection in whole blood-derived platelets by quantitative glucose testing at a university medical center.

    abstract::After the March 2004 implementation of American Association of Blood Banks standards regarding platelet bacterial detection, we began quantitative glucose screening of whole blood-derived platelets (WB-P). The glucose level was measured immediately before component release--often storage day 4 or 5--using the Glucomet...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPVN9OT4GRCSKO

    authors: McKane AV,Ward N,Senn C,Eubanks J,Wessels L,Bowman R

    更新日期:2009-04-01 00:00:00

  • Lack of in vitro interaction between heparin and nitroglycerin.

    abstract::Nitroglycerin has been reported to reduce activated partial thromboplastin time (aPTT) values in patients treated with concurrent heparin and nitroglycerin. However, in vivo studies have yielded conflicting results. In this in vitro evaluation, nitroglycerin was added to samples of pooled plasma from normal volunteers...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/105.3.298

    authors: Barnes AD,Horn JR,Wittkowsky AK

    更新日期:1996-03-01 00:00:00

  • Computer-assisted work station timing analysis of instrument labor efficiency.

    abstract::Labor use ratings assigned to instruments by the Workload Recording Method (WRM) do not change with batch size or walk-away time use. The authors evaluated the effect of both on the labor use of the analyzers Paramax B6100 (Baxter Paramax, Irvine, CA) and Ektachem 700 (Eastman Kodak, Rochester, NY) by timing all worke...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.5.743

    authors: McClellan GA,Nipper HC,Horn MJ,Burris WD,Hodges K,Monaco S,Cox RS Jr

    更新日期:1991-05-01 00:00:00

  • Prospective double-blind clinical trial of bovine, human, and rabbit thromboplastins in monitoring long-term oral anticoagulation.

    abstract::A prospective double-blind trial was performed to investigate the safety of long-term oral anticoagulant control when dosaged adjustment depends on the prothrombin times assessed with one type of thromboplastin being calculated as if assayed by another type. The three main types of thromboplastin, i.e., bovine (Thromb...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1093/ajcp/75.3.297

    authors: Boekhout-Mussert MJ,van der Kolk-Schaap PJ,Hermans J,Loeliger EA

    更新日期:1981-03-01 00:00:00

  • Evaluation of a multitest system for identification of saccharolytic pseudomonads.

    abstract::The API-20 Enteric Strip has been proposed to have the capacity to differentiate the nonfermentative gram-negative bacilli. This multitest strip serves as the basis of an evolving system, which first provided a Register, then a Selector, and more recently a computer Index. Twenty-three strains of Pseudomonas aeruginos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/69.1.41

    authors: Morris MJ,Young VM,Moody MR

    更新日期:1978-01-01 00:00:00

  • Interleukin-2 and interferon-gamma production in follicular lymphomas.

    abstract::In situ hybridization with specific RNA radiolabeled probes was used to analyze the production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) by 21 low-grade follicular lymphomas (FLs). All of the 21 FLs tested contained lymphokine-synthesizing cells in the interfollicular and follicular areas. Enumeration o...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.1.55

    authors: Peuchmaur M,Emilie D,Crevon MC,Brousse N,Gaulard P,D'Agay MF,Galanaud P,Solal-Celigny P

    更新日期:1991-01-01 00:00:00

  • Image cytometric measurement of nuclear proliferation markers (MIB-1, PCNA) in astrocytomas. Prognostic significance.

    abstract::Proliferative activity in astrocytomas, measured by image cytometry, was related to prognosis. Fifty-eight astrocytic neoplasms (grade 1 or 2, 11; grade 3, 31; glioblastoma multiforme, 16) were immunostained for Ki-67 (MIB-1; 1:50) and proliferating cell nuclear antigen (PCNA; prediluted). Proliferative activity (nucl...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/109.1.69

    authors: Kirkegaard LJ,DeRose PB,Yao B,Cohen C

    更新日期:1998-01-01 00:00:00

  • Anaplastic thyroid carcinoma. Immunocytochemical study of 32 cases.

    abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.1.15

    authors: Ordóñez NG,El-Naggar AK,Hickey RC,Samaan NA

    更新日期:1991-07-01 00:00:00

  • Detection of allergic disease in adenoid tissue.

    abstract::Allergic adenoiditis is defined as the presence of numerous brightly fluorescent IgE mast cells (FIEMC) demonstrated in formalin-fixed adenoid tissue by the trypsin-immunofluorescent method. A prospective study of 606 routinely accessioned cases seen during a 39-month period revealed 98 positive cases. Correlation wit...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.2.170

    authors: Loesel LS

    更新日期:1984-02-01 00:00:00

  • Comparison of evaluations for hormone receptors in breast carcinoma using two manual and three automated immunohistochemical assays.

    abstract::The aims of this study were to compare the quality of immunohistochemical assays of estrogen receptor (ER) and progesterone receptor (PR) and to compare the intermethod variability of assays from different manufacturers. immunohistochemical staining was entrusted to the following laboratories in Japan: Kyowa Medex, de...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/4D1A04NCDK96WFY7

    authors: Arihiro K,Umemura S,Kurosumi M,Moriya T,Oyama T,Yamashita H,Umekita Y,Komoike Y,Shimizu C,Fukushima H,Kajiwara H,Akiyama F

    更新日期:2007-03-01 00:00:00

  • The development and testing of a laboratory information system-driven tool for pre-sign-out quality assurance of random surgical pathology reports.

    abstract::We describe the development and testing of a novel pre-sign-out quality assurance tool for case diagnoses that allows for the random review of a percentage of cases by a second pathologist before case verification and release of the final report. The tool incorporates the ability to record and report levels of diagnos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPLN9DU9LNXSXA

    authors: Owens SR,Dhir R,Yousem SA,Kelly SM,Piccoli A,Wiehagen L,Lassige K,Parwani AV

    更新日期:2010-06-01 00:00:00

  • Adamantinoma of the pretibial soft tissue. Clinicopathologic features, differential diagnosis, and possible relationship to intraosseous disease.

    abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.1.108

    authors: Mills SE,Rosai J

    更新日期:1985-01-01 00:00:00

  • Inflammatory myofibroblastic tumor (plasma cell granuloma). Clinicopathologic study of 20 cases with immunohistochemical and ultrastructural observations.

    abstract::Twenty cases of inflammatory myofibroblastic tumor (IMT) were studied; 19 involved the lung and 1 the esophagus only. The patients' ages ranged from 3 to 72 years. There were 9 males and 11 females. Involvement of a bronchus was seen in one case and of mediastinal structures in four. Chest pain and dyspnea were common...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.5.538

    authors: Pettinato G,Manivel JC,De Rosa N,Dehner LP

    更新日期:1990-11-01 00:00:00

  • Hepatic oncocytes. Incidence, staining characteristics, and ultrastructural features.

    abstract::Hepatocytes with orcein-positive ground-glass cytoplasm have been shown to contain abundant hepatitis B surface antigen in the cisternae of excess smooth endoplasmic reticulum. Hepatocytes with similar cytoplasmic changes that did not react with orcein were observed in 26.5% of 49 cases of cirrhosis. These cells exhib...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.498

    authors: Gerber MA,Thung SN

    更新日期:1981-04-01 00:00:00

  • Use of a probe to repeat sequence of the Y chromosome for detection of host cells in peripheral blood of bone marrow transplant recipients.

    abstract::In situ hybridization for the Y chromosome (Y-ISH) was used to identify residual host cells in the peripheral blood of 51 recipients of sex-mismatched allogeneic marrow not depleted of T cells following conditioning with high-dose cyclophosphamide and total body irradiation (TBI). One patient who rejected the graft sh...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.2.201

    authors: Przepiorka D,Thomas ED,Durnam DM,Fisher L

    更新日期:1991-02-01 00:00:00

  • Community-acquired bacteremic Achromobacter xylosoxidans type IIIa pneumonia in a patient with idiopathic IgM deficiency.

    abstract::A man was hospitalized with bacteremic Achromobacter xylosoxidans type IIIa pneumonia. The authors are aware of no previously reported similar infections caused by this bacterium. A clinical cure was achieved with a combination of carbenicillin and kanamycin therapy. Microtiter susceptibility testing revealed that car...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.4.712

    authors: Dworzack DL,Murray CM,Hodges GR,Barnes WG

    更新日期:1978-10-01 00:00:00

  • Prostaglandins in histiocytosis-X. PG synthesis by histiocytosis-X cells.

    abstract::Histiocytosis-X cells were obtained at autopsy from the lungs and lymph nodes of a patient who died of the disseminated infantile form of this disease (Letterer-Siwe disease). The ability of these cells to synthesize and release prostaglandins was investigated in culture, by prelabeling the cell lipids with [14C] arac...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.2.243

    authors: Gonzalez-Crussi F,Hsueh W,Wiederhold MD

    更新日期:1981-02-01 00:00:00

  • Intratumoral genetic heterogeneity in Barrett adenocarcinoma.

    abstract::In 10 cases of Barrett adenocarcinoma, samples from 8 tumor areas (including superficial and deep from peripheral and central areas) and a regional lymph node metastasis were studied for amplification of c-myc, c-erbB-2, and EGFR. We analyzed loss of heterozygosity (LOH) at 3 loci (APC, MCC, and RB) and 2 anonymous mi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/6XY8-FMM5-VVCJ-T3N0

    authors: Owonikoko T,Rees M,Gabbert HE,Sarbia M

    更新日期:2002-04-01 00:00:00

  • Reflex UroVysion testing of bladder cancer surveillance patients with equivocal or negative urine cytology: a prospective study with focus on the natural history of anticipatory positive findings.

    abstract::A proportion of patients under surveillance for recurrent bladder carcinoma with no immediate evidence of bladder tumor recurrence have positive multitarget fluorescence in situ hybridization (FISH; UroVysion, Vysis, Downers Grove, IL) results. The course of these "anticipatory positive" cases and the time to bladder ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/ADJL7E810U1H42BJ

    authors: Yoder BJ,Skacel M,Hedgepeth R,Babineau D,Ulchaker JC,Liou LS,Brainard JA,Biscotti CV,Jones JS,Tubbs RR

    更新日期:2007-02-01 00:00:00

  • Spectrophotometric detection of RT-PCR-amplified BCR/ABL fusion transcripts. A survey performed on archival hematologic slides.

    abstract::We describe a method of spectrophotometric detection of BCR/ABL chimeric sequences amplified by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), enabling the use of archival hematologic slides as RNA sources. Multiplex PCR amplified b3a2, b2a2, and e1a2 break points of the BCR/ABL translocation and ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/108.4.383

    authors: Attadia V,Alosi M,Improta S,Zagonel V,Martinazzo G,Carbone A,Pinto A

    更新日期:1997-10-01 00:00:00

  • Histologic processing of microbiopsies from cervical smears is diagnostically useful in selected cases.

    abstract::We analyzed the value in cervical cytology of a recently developed technique by which it is possible to remove thick tissue specimens, called microbiopsies, from cervical smears and to process them for histologic examination. In 12 (48%) of 25 cervical smears in which microbiopsies were found, the histologic sections ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/108.2.191

    authors: Mravunac M,Verbeek DH,Sanders-Eras M,Besseling R,Wijnen-Dubbers C,Smedts F

    更新日期:1997-08-01 00:00:00

  • Biclonal chronic lymphocytic leukemia.

    abstract::Chronic lymphocytic leukemia (CLL) is well characterized clinically and immunophenotypically. Demonstration of a monotypic CD19+, CD5+ B-cell population is central to the diagnosis. We report 2 cases of biclonal CLL. Two elderly men were encountered with an absolute lymphocytosis consisting of the typical CD5+, CD19+,...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/V8AN-A2XP-7TDV-HR0T

    authors: Hsi ED,Hoeltge G,Tubbs RR

    更新日期:2000-06-01 00:00:00

  • An immunocytochemical study of p53 and bcl-2 protein expression in Hodgkin's disease.

    abstract::In view of their reported reciprocal effects on apoptosis, the expression of p53 and bcl-2 proteins was studied in 46 cases of Hodgkin's disease by immunocytochemical labeling. We found p53 protein in Reed-Sternberg cells and their mononuclear variants in 16 of the 46 cases (34.7%) of Hodgkin's disease, mainly in a nu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/99.6.663

    authors: Doussis IA,Pezzella F,Lane DP,Gatter KC,Mason DY

    更新日期:1993-06-01 00:00:00

  • Usefulness of DNA ploidy measurement on liquid-based smears showing conflicting results between cytology and high-risk human papillomavirus typing.

    abstract::To improve the positive predictive value (PPV) for high-risk human papillomavirus (HR-HPV) in primary screening, DNA ploidy was measured on the same liquid-based sample by image cytometry in 984 cases showing discrepancies between cytology and HR-HPV testing. Of the conflicting results, 14.5% corresponded to a cytolog...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/6NXC-V9XD-YM87-8FAE

    authors: Lorenzato M,Bory JP,Cucherousset J,Nou JM,Bouttens D,Thil C,Dez F,Evrard G,Quereux C,Birembaut P,Clavel C

    更新日期:2002-11-01 00:00:00

  • Peroxidase activity in circulating mast cells in blast crisis of chronic granulocytic leukemia. Comparative studies with basophils and cutaneous mast cells.

    abstract::Although the hematopoietic origin of mast cells is very probable, the cell from which they originate is still a matter of speculation. The description of "transitional basophil/mast cells" in myeloproliferative disorders has suggested a common origin for basophils and mast cells. In a case of mast cell transformation ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.2.212

    authors: Gabriel LC,Escribano LM,Marie JP,Zittoun R,Navarro JL

    更新日期:1986-08-01 00:00:00

  • Increased sensitivity and specificity of Borrelia burgdorferi 16S ribosomal DNA detection.

    abstract::The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sens...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPI72YAXRHYHEE

    authors: Lee SH,Vigliotti VS,Vigliotti JS,Jones W,Pappu S

    更新日期:2010-04-01 00:00:00

  • Differential expression of superoxide dismutases in lung cancer.

    abstract::Oxidant-antioxidant balance is known to regulate growth factors and invasion of tumor cells. Manganese superoxide dismutase (MnSOD), copper zinc SOD (CuZnSOD), and extracellular SOD (ECSOD), the first-line antioxidant defenses, were studied in lung carcinomas by immunohistochemical analysis (n = 139, 56, and 37, respe...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/A45Q-HB0Q-RRX6-CT9A

    authors: Svensk AM,Soini Y,Pääkkö P,Hiravikoski P,Kinnula VL

    更新日期:2004-09-01 00:00:00

  • A cost-effective algorithm for hereditary nonpolyposis colorectal cancer detection.

    abstract::Colorectal cancer with microsatellite instability (MSI) may occur sporadically or be inherited in cases of hereditary nonpolyposis colorectal cancer (HNPCC) syndrome. However, there is no consensus as to which patients must be tested and how to test MSI. In this study, MSI was tested by immunohistochemical analysis an...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/B0AF-DT52-ETMK-EJBE

    authors: Bouzourene H,Taminelli L,Chaubert P,Monnerat C,Seelentag W,Sandmeier D,Andrejevic S,Matter M,Bosman F,Benhattar J

    更新日期:2006-06-01 00:00:00

  • Acetic acid-zinc-formalin: a safe alternative to B-5 fixative.

    abstract::B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, includi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/29DA-CY9K-BHNW-4BG6

    authors: Bonds LA,Barnes P,Foucar K,Sever CE

    更新日期:2005-08-01 00:00:00

  • CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms.

    abstract::We evaluated the immunohistochemical profile and specificity of CD138 reactivity in 238 specimens from hematopoietic and nonhematopoietic neoplasms. In 91 bone marrow biopsies, CD138 reactivity was observed for nonneoplastic plasma cells, neoplastic plasma cells in multiple myeloma cases (43/43), and the plasmacytic c...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/617D-WB5G-NFWX-HW4L

    authors: O'Connell FP,Pinkus JL,Pinkus GS

    更新日期:2004-02-01 00:00:00