Abstract：
:The API-20 Enteric Strip has been proposed to have the capacity to differentiate the nonfermentative gram-negative bacilli. This multitest strip serves as the basis of an evolving system, which first provided a Register, then a Selector, and more recently a computer Index. Twenty-three strains of Pseudomonas aeruginosa and P. putida, 21 of P. fluorescens, 27 of P. maltophilia, and 33 of P. cepacia were investigated by duplicate test runs to determine reproducibility. The results were then evaluated at each of the three stages to determine accuracy of identification and the effect of the system evolution on the identification rate. Seventy-one of the 127 pseudomonads gave reproducible results in the multitest strips. Approximately half of them were correctly grouped or speciated by the Register, whereas 80 to 83% were similarly identified by both the Selector and Index. Of the clinically important pseudomonads, 61 to 65% of P. aeruginosa and 93% of P. maltophilia strains were correctly speciated; neither P. putida nor P. fluorescens strains were speciated, although most were correctly grouped. P. cepacia was speciated by the Index with only 55 to 58% accuracy. Although the present stage of the evolving system offers improvement over the Register, further modifications are needed to eliminate identification problems and to increase speciation.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Morris MJ,Young VM,Moody MRdoi
10.1093/ajcp/69.1.41subject
Has Abstractpub_date
1978-01-01 00:00:00pages
41-7issue
1eissn
0002-9173issn
1943-7722journal_volume
69pub_type
杂志文章abstract：:Anaplastic thyroid carcinomas usually pose no problems in histologic diagnosis because of the obvious invasive growth, high cellularity, and frank anaplasia. Two cases of a variant of anaplastic thyroid carcinoma with peculiar gross and histologic features closely mimicking those of Riedel's thyroiditis are described ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/105.4.388
更新日期：1996-04-01 00:00:00
abstract：:The ability of the cervical Papanicolaou (Pap) smear to detect cervicitis associated with Chlamydia trachomatis was investigated. Cultures and cervical cytology samples were obtained from 487 women seen at the Mayo Clinic Department of Obstetrics and Gynecology and at the Sexually Transmitted Disease clinic of the Olm...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.4.421
更新日期：1983-04-01 00:00:00
abstract：OBJECTIVES:To describe consecutive vulvar biopsy cases and to create an educational template for pathology trainees and practicing pathologists. METHODS:We reviewed 189 consecutive biopsies from the female genital area skin and mucosa. We classified them based on etiologies and examined limited clinical information. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa135
更新日期：2020-09-11 00:00:00
abstract：:An acquired circulating inhibitor to Factor VIII:C was found in a 27-year-old postpartum woman who presented with ecchymoses and hematomas. Postpartum Factor VIII:C inhibitors can clinically manifest with signs and symptoms not unlike those in a classic hemophiliac. The natural history of this inhibitor is typically o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.5.789
更新日期：1982-11-01 00:00:00
abstract：:To investigate the effect of matrix-degrading enzymes on the malignant potential of oral squamous cell carcinoma, the expression of matrix metalloproteinase-2 (MMP-2/72-kD gelatinase/type IV collagenase) in 46 patients who had neck surgery for oral cancer was studied immunohistochemically. In 20 of 26 patients (76.9%)...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.1.18
更新日期：1993-01-01 00:00:00
abstract：:Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.1.35
更新日期：1991-01-01 00:00:00
abstract：:An aggressive visceral form of Kaposi's sarcoma (KS) has been reported in immunosuppressed populations, most notably in patients with acquired immunodeficiency syndrome (AIDS). This presentation contrasts from the indolent skin lesions on the extremities of older males and is distinct enough in the young adult America...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.3.366
更新日期：1986-09-01 00:00:00
abstract：:To study the usefulness of calretinin as an immunohistochemistry marker in the diagnosis of cardiac myxoma (CM) and the origin of myxoma cells, we examined 24 CMs and 9 fetal hearts with immunohistochemical methods on formalin-fixed paraffin-embedded tissues. We compared 24 CMs with 10 mural thrombi, 6 jaw myxomas, an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/NR6G-T872-F090-LBRW
更新日期：2000-11-01 00:00:00
abstract：:Five hundred consecutive isolates of viridans streptococci were identified to the species level in an effort to determine their distribution and incidence in routine clinical specimens. Viridans streptococci accounted for significant percentages of streptococcal isolates from urine, wounds, body fluids, and blood. The...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.6.854
更新日期：1983-12-01 00:00:00
abstract：:The diagnostic value of glypican 3 (GPC3) immunostaining on needle biopsy specimens has not been well assessed. In this study, 120 liver needle biopsy specimens, including 46 from cirrhotic livers and 74 hepatocellular carcinomas (HCCs), were immunohistochemically examined for expression of GPC3. The results showed st...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/WMB5PX57Y4P8QCTY
更新日期：2008-08-01 00:00:00
abstract：:A 37-year-old intravenous drug abuser with acquired immune deficiency syndrome showed elevated activated partial thromboplastin time (APTT) and prothrombin time, normal thrombin time and fibrinogen, and borderline low platelet counts. The patient subsequently had a fracture of the left zygomatic arch, which did not pr...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.4.491
更新日期：1989-04-01 00:00:00
abstract：:The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/YTU2-FUHB-EXJL-WMLD
更新日期：2003-10-01 00:00:00
abstract：:Eight cases are reported of an unusual breast lesion consisting of dense intralobular, perilobular, and perivascular lymphocytic infiltrates associated with lobular atrophy and sclerosis. Immunologic studies on paraffin-embedded tissue (seven cases) and frozen tissue (two cases) demonstrated a predominance of B-cells ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.6.725
更新日期：1990-06-01 00:00:00
abstract：:The cell cycle consists of an initial growth phase (G1), DNA replication (S), a gap phase (G2), and mitosis (M), after which the cell may differentiate or enter the resting state (G0). The cycle is driven by a number of positive and negative regulatory phosphorylation and dephosphorylation events, involving protein ki...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1999-07-01 00:00:00
abstract：:Bleeding problems are symptomatic of platelet delta-storage pool diseases (SPDs) such as Hermansky-Pudlak syndrome. Although at present no cure is available for delta-SPD, early diagnosis is of great importance for prophylactic and supportive treatment. This study tested the usefulness of a flow cytometric assay for p...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/3KRYCPNAPDTVFWGY
更新日期：2007-04-01 00:00:00
abstract：:Terminal transferase (TdT) activity and antigen have been measured in 267 specimens of human bone marrow and peripheral blood by using a biochemical assay for enzymatic activity and an immunofluorescence test for antigen. Oligo p(dA)50 and dGTP were used as reagents in the biochemical assay and either rabbit anti-calf...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/77.4.420
更新日期：1982-04-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection may be complicated by non-Hodgkin's lymphoma. We describe eight cases of B-cell extranodal non-Hodgkin's lymphoma occurring during the course of chronic HCV-related hepatic disease (low-grade of mucosa-associated lymphoid tissue [MALT]-type; diffuse large cell; Burkitt; diffuse small ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/109.5.600
更新日期：1998-05-01 00:00:00
abstract：:We studied the antigen expression profiles of 19 anaplastic large cell lymphoma (ALCL) cases by multiparameter flow cytometry. The neoplastic cells expressed CD45, HLA-DR, and CD30 in all cases. At least 1 T cell-associated antigen was expressed in each case (CD2, 12/17 [71%]; CD4, 12/19 [63%]; CD3, 6/19 [32%]; CD7, 6...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/HEFL-7KC4-35KF-WEX8
更新日期：2003-02-01 00:00:00
abstract：:Over a four-month period, methanol was found in the blood of 18 patients among 687 sequential emergency room admissions screened for alcohols by gas chromatography. In the patients with positive results, blood ethanol ranged from 6 to 570 mg/dL (1.3-123.7 mmol/L), blood methanol from 2.3 to 4.0 mg/dL (0.72-1.25 mmol/L...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/87.6.773
更新日期：1987-06-01 00:00:00
abstract：:In 10 cases of Barrett adenocarcinoma, samples from 8 tumor areas (including superficial and deep from peripheral and central areas) and a regional lymph node metastasis were studied for amplification of c-myc, c-erbB-2, and EGFR. We analyzed loss of heterozygosity (LOH) at 3 loci (APC, MCC, and RB) and 2 anonymous mi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/6XY8-FMM5-VVCJ-T3N0
更新日期：2002-04-01 00:00:00
abstract：:To assess their applied clinical utility, viral cultures and serological tests for cytomegalovirus (CMV) were reviewed at Duke University Medical Center (DUMC), a 1,125-bed tertiary-care hospital. Less than 1% (3 of 1,216) of CMV cultures were positive, and 8% of serum samples (45 of 587) were positive by single sera ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/106.2.180
更新日期：1996-08-01 00:00:00
abstract：:Immunophenotypic analysis is useful in distinguishing reactive from neoplastic lymphoproliferations, particularly when tissue is limited or histologic findings are equivocal. Surface membrane immunoglobulin (SmIg) light chain restriction in B cells is especially helpful in documenting clonality, and the loss of SmIg b...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/5R76B01VCATQLBK6
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVES:BCOR-CCNB3 sarcoma (BCS) is an undifferentiated tumor that has some clinical and morphologic similarity to classic Ewing sarcoma, but it is characterized by a distinct BCOR-CCNB3 gene fusion. There are no reports describing its cytomorphologic findings. METHODS:We describe cytologic findings of five molecul...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz159
更新日期：2020-02-08 00:00:00
abstract：:Factor V Leiden (FVR506Q) is a genetic defect in the factor V (FV) molecule that confers resistance to proteolysis by activated protein C (APC) and is the most common abnormality detected in patients studied for hereditary thrombophilia. The initial screening test for this abnormality was a comparison of the activated...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/109.4.387
更新日期：1998-04-01 00:00:00
abstract：Objectives:To examine the relationship between the recently defined Gleason grade groups and prostate cancer-specific mortality. Methods:If the probability of prostate cancer-specific death is symbolized as P(PSD), the probability of biochemical failure is symbolized as P(BF), and the probability of prostate cancer-sp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw212
更新日期：2017-03-01 00:00:00
abstract：:We compared prothrombin times (PTs) and international normalized ratios (INRs) for blood samples drawn into plastic vs glass collection tubes. We collected 60 venous blood samples into 4.5-mL glass and 2 plastic tubes (2.7 and 3.5 mL). An additional 153 samples, including 63 from warfarin-anticoagulated patients, were...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:The ATHENA (Addressing THE Need for Advanced HPV Diagnostics) HPV study evaluated the clinical usefulness of the cobas HPV Test (Roche Molecular Systems, Pleasanton, CA) for high-risk human papillomavirus (HR-HPV) testing (14 HR types) and individual HPV-16/HPV-18 genotyping in women undergoing routine cervical cytolo...
journal_title：American journal of clinical pathology
pub_type： 临床试验,杂志文章
doi：10.1309/AJCPTUS5EXAS6DKZ
更新日期：2011-10-01 00:00:00
abstract：:Primary osteomyelitis in a teen-aged boy that mimicked Ewing's tumor radiologically showed small Gram-negative rods on the original smear. The organism isolated was an obligately anaerobic bacterium, finally identified as Clostridium sphenoides. This finding underlines the need for microbiologic analysis of orthopedic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.3.385
更新日期：1975-09-01 00:00:00
abstract：:The development of sensitive immunofluorescent technics for the detection of platelet antibodies has stimulated renewed interest in the field of platelet immunology. However, the analysis of multispecific sera containing both HLA and platelet-specific antibodies requires cumbersome absorption technics and relatively l...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.6.839
更新日期：1983-12-01 00:00:00
abstract：OBJECTIVES:To determine the current state of pathology resident training in genomic and molecular pathology. METHODS:The Training Residents in Genomics (TRIG) Working Group developed survey and knowledge questions for the 2013 pathology Resident In-Service Examination (RISE). Sixteen demographic questions related to a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPH2A4XTXJUKDZ
更新日期：2014-10-01 00:00:00
