Abstract:
:The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of PR3 produced in the baculovirus expression system for the detection of PR3-ANCA in 114 patients with systemic vasculitis at diagnosis. We found that ELISA using recombinant PR3 produced in insect cells is a promising alternative for ELISA with native PR3. We found a correlation between tests using recombinant or native PR3, as well as correlation of the ELISA results with ANCA titers measured by the indirect immunofluorescence technique. However, the specificity for ANCA-associated vasculitis of ELISA with recombinant PR3 was lower than ELISA using native PR3. Compared with the direct assay, capture ELISA is a more sensitive method for PR3-ANCA detection, with both native and recombinant PR3, and its results depend on the monoclonal antibody used to capture the antigen.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Rarok AA,Huitema MG,van der Leij MJ,van der Geld YM,Berthold H,Schmitt J,Stegeman CA,Limburg PC,Kallenberg CGdoi
10.1309/YTU2-FUHB-EXJL-WMLDsubject
Has Abstractpub_date
2003-10-01 00:00:00pages
586-95issue
4eissn
0002-9173issn
1943-7722journal_volume
120pub_type
杂志文章abstract:OBJECTIVES:The National Cancer Institute (NCI) National Clinical Trials Network performs phase II and III clinical trials, which increasingly rely on the submission of diagnostic formalin-fixed, paraffin-embedded tissue blocks for biomarker assessment. Simultaneously, advances in precision oncology require that clinica...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz141
更新日期:2020-01-02 00:00:00
abstract::FNAB is the most important advance in the management of thyroid nodules in the past two decades. Dr Mazzaferri has said that finding a thyroid cancer in a thyroid nodule is like finding a needle in a haystack. Fine-needle aspiration biopsy is like applying a strong electromagnet to find that needle. It should be avail...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1997-10-01 00:00:00
abstract::Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.2.177
更新日期:1980-02-01 00:00:00
abstract::An automated fluorescent immunoassay system (FIAX) was compared with complement fixation (CF) for measuring antibodies to cytomegalovirus (CMV) and herpes simplex virus (HSV) in clinical specimens. Compared with CF the specificity of FIAX (ability to detect a negative titer) was 95% for CMV and 92% for HSV. The sensit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/76.3.305
更新日期:1981-09-01 00:00:00
abstract::Extramammary Paget disease (EPD) is an uncommon cutaneous malignant neoplasm that arises in areas rich in apocrine glands (perineum, vulva, and axilla). Apocrine gland origin or apocrine differentiation of cells of EPD has been suggested. Estrongen, progesterone, and androgen hormone receptors have been reported to ex...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/P756-XXCB-TV71-U4XV
更新日期:2000-04-01 00:00:00
abstract::A fatal case of an apparently congenital form of hemophagocytic reticulosis is reported. The onset was manifested by hyperbilirubinemia and hepatosplenomegaly which were present at birth and persisted throughout life. Fever, anemia and pancytopenia developed at 1 month of age and became progressively worse. A splenect...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.4.495
更新日期:1976-04-01 00:00:00
abstract::The reliability of "standard" plasmas used by clinical laboratories to assay factor VIII concentrations of patients' plasmas was studied by assessing the credibility of their reference values. Two of three commercial reference plasmas were considered to be unreliable. The third commercial reference plasma appeared to ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.2.197
更新日期:1981-02-01 00:00:00
abstract::This practice parameter, developed by the Practice Parameters and Outcomes Measurement Committee of the American Society of Clinical Pathologists (ASCP), is a guideline for the development of reasonable stat lists. Stat testing lists vary according to practice setting. Furthermore, although stat lists exist, extenuati...
journal_title:American journal of clinical pathology
pub_type: 指南,杂志文章
doi:10.1093/ajcp/105.6.671
更新日期:1996-06-01 00:00:00
abstract::In the differential diagnosis of Spitz nevus vs malignant melanoma, patient age provides a critical piece of clinical information, because Spitz nevi occur mostly in children and melanomas occur mostly in adults. Nevertheless, there is overlap in the age distributions of Spitz nevus and melanoma. The issue to consider...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/E14C-J6KR-D092-DP3M
更新日期:2004-06-01 00:00:00
abstract::Previous studies suggest that low prostate weight is a significant negative prognostic factor for prostate cancer. In the current study, the data for 431 men who underwent radical retropubic prostatectomy between 1990 and 1998 were analyzed for association between prostate weight and various clinical and pathologic pa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPHGXDI94SGAC
更新日期:2010-04-01 00:00:00
abstract::Histiocytosis X, multicentric reticulohistiocytosis, juvenile xanthogranuloma, the "fibrous" type of dermatofibroma, dermatofibrosarcoma protuberans, and malignant fibrous histiocytoma are all characterized by dermal and/or subcutaneous infiltrates composed at least partially of cells having morphologic features sugge...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.2.162
更新日期:1981-02-01 00:00:00
abstract::Possible increased activation of the coagulation pathway was measured in a group of patients with neoplastic diseases. In addition to standard tests, the thromboplastin generation test, thrombin generation test and immunologic and coagulant activities of both Factor VIII and antithrombin III were utilized in the evalu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/68.2.258
更新日期:1977-08-01 00:00:00
abstract::Previous studies of patients with lymphoid malignancies have suggested a relationship between survival and the presence of more than 40% hard-mirror cells in the bone marrow. This report represents the first detailed study of a patient with American Burkitt's lymphoma with numerous hand-mirror cells in the bone marrow...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.6.937
更新日期:1978-12-01 00:00:00
abstract::Screening for serum lipid disorders is recommended by numerous specialty societies to identify patients at risk for coronary heart disease (CHD). The best screening tests will identify patients at highest risk for CHD who would benefit from intervention. This report discusses an appropriate test panel to use as the in...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.4.380
更新日期:1995-04-01 00:00:00
abstract::The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.3.343
更新日期:1978-09-01 00:00:00
abstract::To assess the usefulness of the cytocentrifuge Gram stain as a urine screening test in the clinical microbiology laboratory for the elimination of culture for screen-negative specimens, we compared the results of the cytocentrifuge Gram stain to the results of culture for 1,171 urine specimens. The data were analyzed ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/108.5.515
更新日期:1997-11-01 00:00:00
abstract:OBJECTIVES:Bone marrows (BMs) with incidentally identified, small monotypic B-cell populations (MBPs) were evaluated. METHODS:BM aspirates with MBPs representing 5% or less of total events by flow cytometry, less than 5.0 × 10(9)/L B cells in blood, and no history of lymphoma or MBP with a different phenotype from pri...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPD5TOBDJU4GKO
更新日期:2014-05-01 00:00:00
abstract::A case of fetal demise and maternal recovery after acetaminophen overdose is presented, to our knowledge the first reported. Fetal liver and maternal serum concentrations indicate overdose to be the cause of fetal death. Maternal disseminated intravascular coagulation (DIC) may have been related to maternal acetaminop...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.2.240
更新日期:1984-08-01 00:00:00
abstract::In the thymus, the relationship between lymphofollicular hyperplasia and mucosa-associated lymphoid tissue (MALT)-type lymphoma is uncertain. We analyzed 14 cases with a diagnosis of thymic follicular hyperplasia in patients with connective tissue disease (n = 2), myasthenia gravis (n = 11), or both (n = 1). In 11 cas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/G3AC-P7K8-2JNK-8C3U
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVES:To differentiate the leprosy agents Mycobacterium leprae and Mycobacterium lepromatosis and correlate them with geographic distribution and clinicopathologic features. METHODS:Species-specific polymerase chain reactions were used to detect each bacillus in archived skin biopsy specimens from patients with l...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP1GLCBE5CDZRM
更新日期:2014-10-01 00:00:00
abstract::Tissue specimens from a series of 46 hepatic tumors occurring in young female oral contraceptive users were tested for alpha1-antitrypsin deposition, utilizing immunocytochemical and histochemical methods. In two instances serum alpha1-antitrypsin phenotyping was also performed. Immunoreactive alpha1-antitrypsin depos...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/68.6.736
更新日期:1977-12-01 00:00:00
abstract::Histologic follow-up of patients with the Bethesda system cervical-vaginal diagnosis of atypical glandular cells of undetermined significance (AGUS), "favor endocervical origin", or "not otherwise specified" (NOS) shows a high percentage of clinically significant (neoplastic or preneoplastic) lesions. Using the criter...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/107.3.299
更新日期:1997-03-01 00:00:00
abstract::Primary massive ovarian edema is uncommon, particularly when it involves both ovaries before puberty. Ovarian edema secondary to a preexisting ovarian lesion is one of the postulated mechanisms. A prepubertal girl presented with bilateral massive ovarian edema with stromal hyperthecosis, which may have predisposed bot...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.5.597
更新日期:1989-05-01 00:00:00
abstract::A 26-year-old woman who had undergone orthotopic heart transplantation because of dilative cardiomyopathy received a triple-drug immunosuppressive regimen (cyclosporine A, azathioprine, and prednisolone). During her relatively frequent episodes of acute rejection, she was treated with methylprednisolone and repeated a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.1.95
更新日期:1990-07-01 00:00:00
abstract::Twenty-four cases of a lesion characterized by unlined spaces in the colorectal lamina propria are presented. While lesions such as this have been reported previously as "lipomatosis" of the colon, histochemical and ultrastructural examination reveals that they are not, in fact, composed of adipocytes. It appears that...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.5.575
更新日期:1985-11-01 00:00:00
abstract::Conventional cytogenetic studies have suggested that trisomy 12 may be a characteristic nonrandom numerical chromosome anomaly in benign ovarian tumors, particularly sex cord-stromal tumors. To confirm this finding, and to avoid possible culture artifact introduced during cytogenetic analysis, the authors performed fl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.6.775
更新日期:1994-12-01 00:00:00
abstract::We studied the histologic and multiparameter flow cytometry (MFC) features of 12 cases of angioimmunoblastic T-cell lymphoma (AITL), 13 of mature T-cell lymphoma, and 25 control cases of reactive lymphoid hyperplasia to evaluate the role of CD10 in the differential diagnosis of peripheral T-cell lymphomas (PTCLs). A c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/MC7QRGPTV0LRR98X
更新日期:2007-11-01 00:00:00
abstract::Pretreatment posterior iliac crest bone marrow biopsies were obtained from 26 multiple myeloma patients. Biopsies were processed in methacrylate. The percentage of myeloma tissue, myeloid tissue, and fat was estimated in each biopsy. In 20 patients who died there was a positive correlation between the percent of myelo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.3.363
更新日期:1983-09-01 00:00:00
abstract:Objectives:Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of hematolymphoid proliferations arising in the context of chronic immunosuppression. The common and indolent B-cell lymphomas, including extranodal marginal zone lymphomas (ENMZLs) of mucosa-associated lymphoid tissue (MALT), are...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/aqx134
更新日期:2017-12-20 00:00:00
abstract::Bronchoalveolar lavage (BAL) is a minimally invasive method possibly representing a diagnostic tool in the evaluation of interstitial lung diseases (ILDs) of different causes. We first describe herein the morphologic, histochemical, and immunohistochemical features of previously unreported eosinophilic globular deposi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPJ6W9XOFEXIYS
更新日期:2008-12-01 00:00:00