Abstract:
:Terminal transferase (TdT) activity and antigen have been measured in 267 specimens of human bone marrow and peripheral blood by using a biochemical assay for enzymatic activity and an immunofluorescence test for antigen. Oligo p(dA)50 and dGTP were used as reagents in the biochemical assay and either rabbit anti-calf TdT or rabbit anti-human TdT was used as the primary antibody for immunofluorescence. Because both false-positive and false-negative detection of TdT antigen occurs, the biochemical assay of TdT activity is considered the standard against which immunofluorescence assays must be measured. If specimens of cells contained TdT activity, then the immunofluorescence detected antigen in 91% of cases (rabbit anti-calf TdT) and 95% of cases (rabbit anti-human TdT). When no TdT activity was detected, the immunofluorescence test was positive in 7.8% of cases (rabbit anti-calf TdT) and 5.2% of cases (rabbit anti-human TdT). When air-dried slices were shipped by air mail to a distant location before being stained for immunofluorescence, TdT antigen was detected in only 33% of matched pair cases which contained TdT activity. From this study, the authors conclude that with current methodology, immunofluorescence tests for TdT antigen must be carried out on slides prepared in the testing laboratory and that such tests are reliable in more than 90% of cases. However, because a small percentage of results are false positives and false negatives, the authors suggest that if a patient's clinical response is not consistent with the immunofluorescence TdT result, an enzymatic assay for TdT activity be carried out.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Cibull ML,Coleman MS,Nelson O,Hutton JJ,Gordon D,Bollum FJdoi
10.1093/ajcp/77.4.420subject
Has Abstractpub_date
1982-04-01 00:00:00pages
420-3issue
4eissn
0002-9173issn
1943-7722journal_volume
77pub_type
杂志文章abstract::Immunostaining of cell cycle-related antigens, especially Ki-67, DNA polymerase alpha, and proliferating cell nuclear antigen, has become an important method to assess the proliferative activity of tumors. These three nuclear antigens were studied by immunohistochemical analysis of cytologic smears. These smears were ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/98.2.161
更新日期:1992-08-01 00:00:00
abstract::While plasmacytosis is a common occurrence in early post-bone marrow transplantation biopsy specimens, the significance of plasma cells in such specimens from patients with multiple myeloma (MM) is unknown. We attempted to retrospectively determine, by morphologic assessment of plasma cell percentage, immunohistologic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/107.6.643
更新日期:1997-06-01 00:00:00
abstract::This article explores 3 aspects of breast diagnosis that are currently under investigation and about which our thinking has recently undergone considerable reshaping. The trend toward more frequent evaluation of clinically subtle lesions has suggested that it might be necessary to understand thefine-needle aspiration ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/29FP-0QAU-CMJY-BK3U
更新日期:2000-05-01 00:00:00
abstract::An assessment of the infrequently performed protein-bound iodine determinations (PBI) and thyroxine (T4) determinations by column chromatography is presented after analysis of data contributed by participants in the 1976 College of American Pathologists Chemistry Survey Programs. The two technics yield comparable mean...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract:OBJECTIVES:To assess physicians' and nurses' satisfaction with the service provided by the laboratory at Gondar University Hospital. METHODS:We conducted a cross-sectional study involving 196 nurses and physicians. RESULTS:Overall level of satisfaction was 51.1% for nurses and 51.5% for physicians. Lack of consistenc...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPU1PLVOIN5JQI
更新日期:2013-09-01 00:00:00
abstract::We assessed cytologic specimens from 11 mantle cell lymphomas (MCLs) and 32 other B-cell non-Hodgkin lymphomas (NHLs) for 11q13 breakpoints using a 2-color fluorescence in situ hybridization (FISH) assay that uses an 11q13 probe centered on the CCND1 gene and a centromeric chromosome 11 probe (CEP11). The number of nu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/69EJ-RFM5-E976-BUTP
更新日期:2000-08-01 00:00:00
abstract::Terminal deoxynucleotidyl transferase (TdT) is a marker of lymphoblastic disorders. Previously, the technique for determining TdT on smears was by indirect immunofluorescence. An immunoperoxidase procedure for detecting TdT on smears is reported. Results are in a comparable range with the indirect immunofluorescent te...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.2.174
更新日期:1982-02-01 00:00:00
abstract::Previous reports have indicated that patients with severe acute respiratory syndrome (SARS)-associated coronavirus infection could develop atypical pneumonia with fulminant pulmonary edema. However, the target cells of SARS viral infection have not been characterized in detail. We report the pathologic findings of the...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/C0ED-U0RA-QBTX-BHCE
更新日期:2004-04-01 00:00:00
abstract::Extramammary Paget disease (EPD) is an uncommon cutaneous malignant neoplasm that arises in areas rich in apocrine glands (perineum, vulva, and axilla). Apocrine gland origin or apocrine differentiation of cells of EPD has been suggested. Estrongen, progesterone, and androgen hormone receptors have been reported to ex...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/P756-XXCB-TV71-U4XV
更新日期:2000-04-01 00:00:00
abstract::The nephrotic syndrome has long been recognized as a hypercoagulable state. Arterial thrombosis is a rare complication of the syndrome. Diuretics and steroids, standard treatment for exacerbations, have been implicated as contributing to the development of arterial thrombosis. The authors present the pathologic, clini...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/101.2.230
更新日期:1994-02-01 00:00:00
abstract::A test for the detection of antibodies to HTLV-III is available and will be widely used to screen donated blood to prevent transfusion-associated acquired immunodeficiency syndrome (AIDS). Based upon the sensitivity and specificity, the authors calculated the expected predictive values for different groups of asymptom...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.6.700
更新日期:1986-06-01 00:00:00
abstract::We previously reported 24 cases of marrow fibrin-ring granuloma (FRG) encountered in 1 institution and concluded that, contrary to previous studies showing marrow FRG as a diagnostic marker for Q fever, Epstein-Barr virus (EBV) was the most common proven cause of such FRG. The present study characterized patients with...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPB7SX7QXASPSK
更新日期:2010-02-01 00:00:00
abstract::Intrauterine pulmonary infarction is extremely rare. In a series of 775 stillborn and newborn infants, only a few cases of acute pulmonary infarction were reported. The authors present the findings from a full-term infant who died at 9 hours of age of a chronic pulmonary infarction. Several pathogenic mechanisms, incl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/100.6.701
更新日期:1993-12-01 00:00:00
abstract::Double immunostaining of tissue sections by monoclonal antibodies has been performed using, in immunofluorescence, combinations of reagents conjugated with different fluorochromes. These were monoclonal antibodies directly labeled with phycoerythrin (a new red fluorochrome) and haptenated monoclonal antibodies detecte...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.1.44
更新日期:1984-07-01 00:00:00
abstract::Several laboratory methods are currently available for the detection of barbiturates, but few are suitable for emergency use, The authors evaluated use of an ultraviolet spectrophotometric method as a screening procedure and compared the results with results obtained by other barbiturate methods (colorimetric, gas-li...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.5.823
更新日期:1976-11-01 00:00:00
abstract::The Philadelphia chromosome (Ph) is found in most chronic myelogenous leukemia (CML) patients. The bcr-abl oncoprotein (P210 or P185), the product of the fused bcr-abl gene produced by the Ph, is known to be the major factor in initiation and maintenance of the leukemic state in these types of leukemias. The authors h...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.4.442
更新日期:1996-10-01 00:00:00
abstract::Nitroglycerin has been reported to reduce activated partial thromboplastin time (aPTT) values in patients treated with concurrent heparin and nitroglycerin. However, in vivo studies have yielded conflicting results. In this in vitro evaluation, nitroglycerin was added to samples of pooled plasma from normal volunteers...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/105.3.298
更新日期:1996-03-01 00:00:00
abstract::Seventeen tubular carcinomas occurred among 211 consecutive mastectomies (7.6%). The relative frequencies of multicentric involvement in the ipsilateral breast (56%), history of bilateral mammary cancer (38%) (P less than .01), and family history of mammary cancer in a first-degree relative (40%) (P less than .05) wer...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.1.25
更新日期:1980-01-01 00:00:00
abstract::Reducing the rate of avoidable errors is crucial to patient safety. Telephone calls with misunderstood critical results constitute one area in which opportunities for improvement exist. The aviation industry has dealt with this issue by requiring pilots to repeat instructions received from the air traffic controller. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/9DYM-6R0T-M830-U95Q
更新日期:2004-06-01 00:00:00
abstract::Primary thymic epithelial neoplasms have been the subject of much controversy in recent years owing to the difficulties posed by these tumors for precise histopathologic typing and prognostication. A number of classification schemes using different terminology have been proposed, none of which has satisfactorily manag...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/111.6.826
更新日期:1999-06-01 00:00:00
abstract:OBJECTIVES:To determine the public health surveillance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing volume needed, both for acute infection and seroprevalence. METHODS:Required testing volumes were developed using standard statistical methods based on test analytical performance, disease preval...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa099
更新日期:2020-07-07 00:00:00
abstract:OBJECTIVES:Pure erythroid leukemia (PEL) is exceptionally rare in the pediatric setting. Four pediatric PEL cases with t(1;16)(p31;q24) NFIA-CBFA2T3 were reported previously. We present a case of an infant with PEL presenting with erythroblastic sarcoma and harboring a novel t(1;8)(p31.3;q21.3) NFIA-RUNX1T1 fusion dete...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa216
更新日期:2020-12-14 00:00:00
abstract::Mechanical release of single cell suspensions from solid tumors for DNA analysis by flow cytometry has been shown to be optimal for adenocarcinomas in general. In the breast, many adenocarcinomas are fibrotic or scirrhous, creating difficulty in separating the neoplastic cells from the stroma. The authors have conduct...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.1.8
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVES:To investigate metastasis associated in colon cancer 1 (MACC1) and MET expression in colorectal adenoma, Tis, early-stage invasive (T1 and T2), and advanced adenocarcinoma with liver metastasis using immunohistochemistry. METHODS:Ninety-three paraffin-embedded colorectal tumor specimens were immunohistochem...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPRH1H5RWWSXRB
更新日期:2013-11-01 00:00:00
abstract::Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.3.512
更新日期:1976-09-01 00:00:00
abstract::A case is presented of an anterior mediastinal mass arising in a 64-year-old woman that showed on histologic, immunopathologic, and ultra-structural examination features of a poorly differentiated (insular) thyroid carcinoma. The tumor in this patient most likely arose from ectopically displaced thyroid tissue on the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.4.408
更新日期:1995-10-01 00:00:00
abstract::Automation was introduced into the clinical microbiology laboratory in the 1960s but initially met with limited success. Today, instruments are an integral part of many clinical laboratories and are used for microbial detection, identification, and susceptibility testing; detection of positive blood cultures; screenin...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1992-10-01 00:00:00
abstract::We conducted this study to ascertain the prevalence of erb-b2 gene amplification in breast cancer specimens read as 2+ in immunohistochemical analysis. Slides from patients with metastatic or recurrent breast cancer were eligible for fluorescent in situ hybridization (FISH) study if they were read as 2+ immunohistoche...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/R2X4KK22QCL7PLME
更新日期:2005-07-01 00:00:00
abstract::Mean nuclear areas (MNA) were calculated morphometrically in ten cases of well-differentiated adenocarcinoma, ten cases of atypical alveolar cuboidal cell hyperplasia (AAH), and five cases of alveolar cuboidal cell hyperplasia (AH) and were compared with each other. In cases of adenocarcinoma, the MNA were significant...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.2.146
更新日期:1986-02-01 00:00:00
abstract::The Society for Hematopathology and European Association for Haematopathology workshop, from October 27 to 29, 2011, in Los Angeles, CA, exhibited many exemplary skin biopsy specimens with interesting inflammatory changes mimicking features of cutaneous lymphoma. This article reviews features observed in cutaneous lym...
journal_title:American journal of clinical pathology
pub_type: 共识发展会议,杂志文章
doi:10.1309/AJCPX4BXTP2QBRKO
更新日期:2013-04-01 00:00:00