Ultraviolet spectrophotometric analysis of barbiturates. Current assessment as an emergency screening procedure.

Abstract:

:Several laboratory methods are currently available for the detection of barbiturates, but few are suitable for emergency use, The authors evaluated use of an ultraviolet spectrophotometric method as a screening procedure and compared the results with results obtained by other barbiturate methods (colorimetric, gas-liquid chromatographic, and immunochemical), Procedure time was approximately 40 minutes, sensitivity 0.2 mg/dl, precision (coefficient of variation) 5.4%, recovery 97.8% for phenobarbital, and there was good correlation with results obtained by gas-liquid chromatography and the EMIT system for phenobarbital. The authors recommend ultraviolet spectrophotometry as an excellent screening procedure for assessing the severity of barbiturate intoxication.

journal_name

Am J Clin Pathol

authors

Schumann GB,Lauenstein K,LeFever D,Henry JB

doi

10.1093/ajcp/66.5.823

subject

Has Abstract

pub_date

1976-11-01 00:00:00

pages

823-30

issue

5

eissn

0002-9173

issn

1943-7722

journal_volume

66

pub_type

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