Detection of cytomegalovirus antibodies in serum using the TranSTAT-CMV and CMV Scan assays.

Abstract:

:Two hundred serum samples were tested with two assays for rapid detection of cytomegalovirus (CMV) antibodies. The assays were CMV Scan, a two-step latex particle agglutination assay for CMV IgG and IgM antibodies, and TranSTAT-CMV, a newly available six-step solid-phase enzyme immunoassay for CMV IgG antibodies. Comparison of initial results to reference results revealed a sensitivity/specificity of 97.2%/96.8% for CMV Scan and 94.3%/83.0% for TranSTAT-CMV. The same comparison after testing discrepant samples in duplicate increased the sensitivity/specificity to 99.1/100% for CMV Scan and 100%/93.6% for TranSTAT-CMV. Borderline-positive results were observed much more frequently with TranSTAT-CMV than CMV Scan (35 vs. 2), compromising easy interpretation of results. CMV Scan is superior to TranSTAT-CMV on the basis of performance characteristics, simplicity of test performance, and ease of interpretation of results.

journal_name

Am J Clin Pathol

authors

Roseff SD,Campos JM

doi

10.1093/ajcp/99.5.539

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

539-41

issue

5

eissn

0002-9173

issn

1943-7722

journal_volume

99

pub_type

杂志文章
  • Are en face frozen sections accurate for diagnosing margin status in melanocytic lesions?

    abstract::To assess the diagnostic accuracy of margin evaluation of melanocytic lesions using en face frozen sections compared with standard paraffin-embedded sections, we studied 2 sets of lesions in which en face frozen sections were used for analysis of surgical margins (13 from malignant melanomas [MMs] and 10 from nonmelan...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/J1Q0-V35E-UTMV-R193

    authors: Prieto VG,Argenyi ZB,Barnhill RL,Duray PH,Elenitsas R,From L,Guitart J,Horenstein MG,Ming ME,Piepkorn MW,Rabkin MS,Reed JA,Selim MA,Trotter MJ,Johnson MM,Shea CR

    更新日期:2003-08-01 00:00:00

  • Aberrant expression of CD7 in myeloblasts is highly associated with de novo acute myeloid leukemias with FLT3/ITD mutation.

    abstract::Acute myeloid leukemia (AML) with normal cytogenetics represents approximately 40% to 50% of de novo AML. This heterogeneous AML subgroup constitutes the single largest cytogenetic group with an intermediate prognosis. Previous studies have suggested that the Fms-like tyrosine kinase-3 internal tandem duplication (FLT...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/NRTX9AKXHR5JBT93

    authors: Rausei-Mills V,Chang KL,Gaal KK,Weiss LM,Huang Q

    更新日期:2008-04-01 00:00:00

  • Hypoglycorrhachia and coxsackie B3 meningoencephalitis.

    abstract::The case of a 3-week-old infant with Coxsackie B3 meningoencephalitis is discussed. Hypoglycorrhachia and neutrophils in the cerebrospinal fluid characterized the first ten days of her illness. Coxsackie B3 should be added to the list of enteroviruses that can cause hypoglycorrhachia during infections of the central n...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.6.947

    authors: Chesney PJ,Quennec P,Clark C

    更新日期:1978-12-01 00:00:00

  • Band neutrophil counts are unnecessary for the diagnosis of infection in patients with normal total leukocyte counts.

    abstract::The authors have assessed the clinical value of an "accurate" band count as a correlate to infection in 33 patients with a culture proven bacterial infection and a normal total leukocyte count. Thirty hematologically normal outpatients, presumably with no infections, were selected for the control group. Manual 500 cel...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/102.5.646

    authors: Ardron MJ,Westengard JC,Dutcher TF

    更新日期:1994-11-01 00:00:00

  • Improving quality of patient care in an emergency department: a laboratory perspective.

    abstract::The purpose of our study was to improve the quality of care in an emergency department (ED) as measured by length of stay (LOS), total turnaround time (TAT) for laboratory result reporting, and the blood culture contamination rate. Data were included for patients who had at least 1 of 5 laboratory tests performed as p...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/DGXYTH0VNTTQRQHD

    authors: Sheppard C,Franks N,Nolte F,Fantz C

    更新日期:2008-10-01 00:00:00

  • Anaplastic thyroid carcinoma. Immunocytochemical study of 32 cases.

    abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.1.15

    authors: Ordóñez NG,El-Naggar AK,Hickey RC,Samaan NA

    更新日期:1991-07-01 00:00:00

  • The current state of resident training in genomic pathology: a comprehensive analysis using the resident in-service examination.

    abstract:OBJECTIVES:To determine the current state of pathology resident training in genomic and molecular pathology. METHODS:The Training Residents in Genomics (TRIG) Working Group developed survey and knowledge questions for the 2013 pathology Resident In-Service Examination (RISE). Sixteen demographic questions related to a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPH2A4XTXJUKDZ

    authors: Haspel RL,Rinder HM,Frank KM,Wagner J,Ali AM,Fisher PB,Parks ER,Training Residents in Genomics (TRIG) Working Group.

    更新日期:2014-10-01 00:00:00

  • Immunochemical detection of human blood in feces.

    abstract::Current methods for testing stool samples for hemoglobin utilize peroxidase oxidation of chemical indicators such as guaiac or benzidine. These tests have frequent false-positive and false-negative results, complicating random screening for occult gastrointestinal bleeding. The authors have developed an immunochemical...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/69.1.342

    authors: Barrows GH,Burton RM,Jarrett DD,Russell GG,Alford MD,Songster CL

    更新日期:1978-03-01 00:00:00

  • Aberrations of chromosomes 9 and 17 in bilharzial bladder cancer as detected by fluorescence in situ hybridization.

    abstract::Fluorescence in situ hybridization (FISH), using repetitive alpha satellite probes to chromosomes 9 and 17, was performed on 27 paraffinembedded bladder specimens from 18 Egyptian patients affected with bilharzial carcinoma. Fluorescence in situ hybridization results in the carcinomas and benign mucosa of patients wit...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.2.234

    authors: Ghaleb AH,Pizzolo JG,Melamed MR

    更新日期:1996-08-01 00:00:00

  • A comprehensive evaluation of the performance of duplicate prothrombin time and activated partial thromboplastin time assays.

    abstract::An evaluation of the performance of duplicate prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays was undertaken to develop analytical duplicate performance criteria in order to quantitate the risks associated with singlet versus duplicate procedures. Data were retrospectively collected from ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/85.4.456

    authors: Scheer WD,Catrou PG,Lipscomb GE,Boudreau DA

    更新日期:1986-04-01 00:00:00

  • High percentage of JAK2 exon 12 mutation in Asian patients with polycythemia vera.

    abstract::We examined the occurrence of JAK2(V617F) and JAK2 exon 12 mutations in a clinical cohort of polycythemia vera (PV) in Taiwan. Of 22 patients with PV, 17 (77%) had the V617F mutation, and all 5 V617F-negative patients (23%) had the exon 12 mutation. We found 3 different exon 12 mutations: 3 N542-E543del, 1 F537-K539de...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPK7KGOWPHYWM0

    authors: Yeh YM,Chen YL,Cheng HY,Su WC,Chow NH,Chen TY,Ho CL

    更新日期:2010-08-01 00:00:00

  • The defined antigen substrate sphere system with direct immunohistoperoxidase for detection of soluble dengue antigen in sera of patients with dengue hemorrhagic fever.

    abstract::The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.3.343

    authors: Boonpucknavig S,Bunnag C,Vuttiviroj O,Nimmannitya S

    更新日期:1978-09-01 00:00:00

  • Protein C assay performance: an analysis of North American specialized coagulation laboratory association proficiency testing results.

    abstract::To determine the performance and frequency of protein C reagents currently used by clinical laboratories, we analyzed North American Specialized Coagulation Laboratory Association (NASCOLA) protein C proficiency testing data from 6 surveys conducted in 2009 and 2010 (2009-1 to 2009-3 and 2010-1 to 2010-3). Interlabora...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP8MWU4QSTCLPU

    authors: Baron JM,Johnson SM,Ledford-Kraemer MR,Hayward CP,Meijer P,Van Cott EM

    更新日期:2012-06-01 00:00:00

  • Giant-cell arteritis of the uterus. Report of two cases.

    abstract::Two cases of giant-cell arteritis of uterus were found incidentally in association with uterine prolapse. One similar case has been reported in the literature. Microscopic examination disclosed fragmentation of the elastic laminae of the myometrial arteries with a giant-cell and mononuclear infiltrate. The relationshi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/65.3.308

    authors: Pirozynski WJ

    更新日期:1976-03-01 00:00:00

  • Detection of Falsely Elevated Point-of-Care Potassium Results Due to Hemolysis Using Predictive Analytics.

    abstract:OBJECTIVES:Preanalytical factors, such as hemolysis, affect many components of a test panel. Machine learning can be used to recognize these patterns, alerting clinicians and laboratories to potentially erroneous results. In particular, machine learning might identify which cases of elevated potassium from a point-of-c...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa039

    authors: Benirschke RC,Gniadek TJ

    更新日期:2020-07-07 00:00:00

  • Cross-reactivity with normal antigens in commercial anti-CEA sera, used for immunohistology. The need for tissue controls and absorptions.

    abstract::The demonstration of carcinoembryonic antigen (CEA) in serum and in tissue sections may be of value in diagnosis and follow-up of several malignant tumors. Anti-CEA sera, however, cross-react with normal antigens, also present in tumors. The staining patterns of three commercially produced anti-CEA sera were investiga...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.1.25

    authors: Nap M,ten Hoor KA,Fleuren GJ

    更新日期:1983-01-01 00:00:00

  • Bladder Cancer Involving Smooth Muscle of Indeterminate Type or Muscularis Mucosae in Transurethral Biopsy Specimens.

    abstract:OBJECTIVES:Bladder cancers invading the muscularis mucosae (MM) are treated differently from those invading the muscularis propria (MP). However, it may be difficult to determine the type of smooth muscle in transurethral resection (TUR) or biopsy specimens. We aimed to investigate the clinicopathologic features of bla...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa030

    authors: Hwang MJ,Kamat AM,Dinney CP,Czerniak B,Guo CC

    更新日期:2020-07-07 00:00:00

  • Automated immunohistochemical assay for estrogen receptor status in breast cancer using monoclonal antibody CC4-5 on the Ventana ES.

    abstract::Determination of breast cancer estrogen receptor (ER) status as a predictor of tumor response to adjuvant endocrine therapy remains a mainstay of breast cancer management. Recent second generation anti-ER antibodies and new epitope retrieval methods have produced paraffin-based immunohistochemical results that correla...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.3.332

    authors: Nichols GE,Frierson HF Jr,Boyd JC,Hanigan MH

    更新日期:1996-09-01 00:00:00

  • A rapid paper-disc test for penicillinase.

    abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.557

    authors: Tu KK,Jorgensen JH,Stratton CW

    更新日期:1981-04-01 00:00:00

  • Neonatal Intensive Care Unit Quality Initiative:  Identifying Preanalytical Variables Contributing to Specimen Hemolysis and Measuring the Impact of Evidence-Based Practice Interventions.

    abstract:OBJECTIVES:Blood specimen hemolysis is a major cause of sample recollection in the neonatal intensive care unit. We aimed to reduce the hemolysis rate from 6.3% at baseline to less than 4% within the 9 months' duration of the study. METHODS:Intravenous infusion of lipid emulsion during sample collection, sample collec...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqw086

    authors: Tolan NV,Kaleta EJ,Fang JL,Colby CE,Carey WA,Karon BS,Baumann NA

    更新日期:2016-07-01 00:00:00

  • ANA testing: a comparison of two kits and a discussion of specificity and sensitivity as criteria for selection of screening procedures.

    abstract::A comparative evaluation of two commercial kits that detect antinuclear antibodies via indirect immunofluorescence was undertaken using an adult ambulatory population. The sensitivity and specificity of the kits were compared to determine which of the two was more efficient as a screening test for systemic lupus eryth...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.3.308

    authors: Valdiserri RO,Petronie JS

    更新日期:1984-03-01 00:00:00

  • MUC4 expression increases progressively in pancreatic intraepithelial neoplasia.

    abstract::Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/7Y7N-M1WM-R0YK-M2VA

    authors: Swartz MJ,Batra SK,Varshney GC,Hollingsworth MA,Yeo CJ,Cameron JL,Wilentz RE,Hruban RH,Argani P

    更新日期:2002-05-01 00:00:00

  • An automated method for the analysis of T-cell receptor repertoires. Rapid RT-PCR fragment length analysis of the T-cell receptor beta chain complementarity-determining region 3.

    abstract::The examination of T-cell receptor (TCR) repertoires has an important role in the study of lymphoproliferative disorders and autoimmune diseases. Analysis of the complementarity-determining region 3 (CDR3) of the TCR beta chain is used to assess the clonality of T-cell populations. We developed a rapid fluorescence-ba...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/111.5.683

    authors: Lue C,Mitani Y,Crew MD,George JF,Fink LM,Schichman SA

    更新日期:1999-05-01 00:00:00

  • A rare lymphoepithelial cyst of the pancreas.

    abstract::Reported is a case of a rare lymphoepithelial cyst of the tail of the pancreas that developed in a young man with no symptoms. To the authors' knowledge, it is the first case described to be entirely situated within the pancreas, thus confirming its pancreatic origin. Biologic behavior appears to be entirely benign, a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/98.2.188

    authors: DiCorato MP,Schned AR

    更新日期:1992-08-01 00:00:00

  • Restriction fragment length polymorphism and polymerase chain reaction: HLA-DQA1 and polymarker analysis of blood samples from transfusion recipients.

    abstract::The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polymarker (LDLR, GYPA, ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/KAWA-QHBU-6D1J-XY3G

    authors: Giroti RI,Biswas R,Mukherjee K

    更新日期:2002-09-01 00:00:00

  • Hepatitis B (surface) antigen testing by radioimmunoassaymexperience in a very large volunteer donor population.

    abstract::The results of hepatitis B surface antigen (HBs-Ag) testing in a large volunteer blood donor population are described. Counterelectrophoresis and three versions of solid-phase radioimmunoassay technic are compared and evaluated. Initial results suggested that the radioimmunoassay technic are compared and evaluated. In...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/63.6.847

    authors: Dodd RY,Ni LY,Mallin WS,Greenwalt TJ

    更新日期:1975-06-01 00:00:00

  • Does the size matter?: Prostate weight does not predict PSA recurrence after radical prostatectomy.

    abstract::Previous studies suggest that low prostate weight is a significant negative prognostic factor for prostate cancer. In the current study, the data for 431 men who underwent radical retropubic prostatectomy between 1990 and 1998 were analyzed for association between prostate weight and various clinical and pathologic pa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPPHGXDI94SGAC

    authors: Davidson DD,Koch MO,Lin H,Jones TD,Biermann K,Cheng L

    更新日期:2010-04-01 00:00:00

  • Pseudo-sulfur granules associated with intrauterine contraceptive devices.

    abstract::A structure resembling a sulfur granule found in histologic sections of endometrium from patients with intrauterine contraceptive devices is described. It is distinguished from a true sulfur granule on morphologic grounds and is further characterized as a foreign substance, most probably fragments of the synthetic con...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.6.822

    authors: O'Brien PK,Roth-Moyo LA,Davis BA

    更新日期:1981-06-01 00:00:00

  • Uterine tumors resembling ovarian sex-cord tumors. A clinicopathologic analysis of fourteen cases.

    abstract::Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/66.3.512

    authors: Clement PB,Scully RE

    更新日期:1976-09-01 00:00:00

  • Spurious red blood cell parameters due to serum cold agglutinins: observations on Ortho ELT-8 cell counter.

    abstract::The pattern of spurious red blood cell parameters caused by serum cold agglutinins has been well characterized for the Coulter counters but not the Ortho ELT-8. The pattern consists of a spurious increase in mean corpuscular volume (MCV), a discrepancy between the hemoglobin and the hematocrit, the latter falsely redu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.2.218

    authors: Solanki DL,Blackburn BC

    更新日期:1985-02-01 00:00:00