Abstract:
:Two hundred serum samples were tested with two assays for rapid detection of cytomegalovirus (CMV) antibodies. The assays were CMV Scan, a two-step latex particle agglutination assay for CMV IgG and IgM antibodies, and TranSTAT-CMV, a newly available six-step solid-phase enzyme immunoassay for CMV IgG antibodies. Comparison of initial results to reference results revealed a sensitivity/specificity of 97.2%/96.8% for CMV Scan and 94.3%/83.0% for TranSTAT-CMV. The same comparison after testing discrepant samples in duplicate increased the sensitivity/specificity to 99.1/100% for CMV Scan and 100%/93.6% for TranSTAT-CMV. Borderline-positive results were observed much more frequently with TranSTAT-CMV than CMV Scan (35 vs. 2), compromising easy interpretation of results. CMV Scan is superior to TranSTAT-CMV on the basis of performance characteristics, simplicity of test performance, and ease of interpretation of results.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Roseff SD,Campos JMdoi
10.1093/ajcp/99.5.539subject
Has Abstractpub_date
1993-05-01 00:00:00pages
539-41issue
5eissn
0002-9173issn
1943-7722journal_volume
99pub_type
杂志文章abstract::To assess the diagnostic accuracy of margin evaluation of melanocytic lesions using en face frozen sections compared with standard paraffin-embedded sections, we studied 2 sets of lesions in which en face frozen sections were used for analysis of surgical margins (13 from malignant melanomas [MMs] and 10 from nonmelan...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/J1Q0-V35E-UTMV-R193
更新日期:2003-08-01 00:00:00
abstract::Acute myeloid leukemia (AML) with normal cytogenetics represents approximately 40% to 50% of de novo AML. This heterogeneous AML subgroup constitutes the single largest cytogenetic group with an intermediate prognosis. Previous studies have suggested that the Fms-like tyrosine kinase-3 internal tandem duplication (FLT...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/NRTX9AKXHR5JBT93
更新日期:2008-04-01 00:00:00
abstract::The case of a 3-week-old infant with Coxsackie B3 meningoencephalitis is discussed. Hypoglycorrhachia and neutrophils in the cerebrospinal fluid characterized the first ten days of her illness. Coxsackie B3 should be added to the list of enteroviruses that can cause hypoglycorrhachia during infections of the central n...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.6.947
更新日期:1978-12-01 00:00:00
abstract::The authors have assessed the clinical value of an "accurate" band count as a correlate to infection in 33 patients with a culture proven bacterial infection and a normal total leukocyte count. Thirty hematologically normal outpatients, presumably with no infections, were selected for the control group. Manual 500 cel...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.5.646
更新日期:1994-11-01 00:00:00
abstract::The purpose of our study was to improve the quality of care in an emergency department (ED) as measured by length of stay (LOS), total turnaround time (TAT) for laboratory result reporting, and the blood culture contamination rate. Data were included for patients who had at least 1 of 5 laboratory tests performed as p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/DGXYTH0VNTTQRQHD
更新日期:2008-10-01 00:00:00
abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.15
更新日期:1991-07-01 00:00:00
abstract:OBJECTIVES:To determine the current state of pathology resident training in genomic and molecular pathology. METHODS:The Training Residents in Genomics (TRIG) Working Group developed survey and knowledge questions for the 2013 pathology Resident In-Service Examination (RISE). Sixteen demographic questions related to a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPH2A4XTXJUKDZ
更新日期:2014-10-01 00:00:00
abstract::Current methods for testing stool samples for hemoglobin utilize peroxidase oxidation of chemical indicators such as guaiac or benzidine. These tests have frequent false-positive and false-negative results, complicating random screening for occult gastrointestinal bleeding. The authors have developed an immunochemical...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/69.1.342
更新日期:1978-03-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH), using repetitive alpha satellite probes to chromosomes 9 and 17, was performed on 27 paraffinembedded bladder specimens from 18 Egyptian patients affected with bilharzial carcinoma. Fluorescence in situ hybridization results in the carcinomas and benign mucosa of patients wit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.2.234
更新日期:1996-08-01 00:00:00
abstract::An evaluation of the performance of duplicate prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays was undertaken to develop analytical duplicate performance criteria in order to quantitate the risks associated with singlet versus duplicate procedures. Data were retrospectively collected from ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.4.456
更新日期:1986-04-01 00:00:00
abstract::We examined the occurrence of JAK2(V617F) and JAK2 exon 12 mutations in a clinical cohort of polycythemia vera (PV) in Taiwan. Of 22 patients with PV, 17 (77%) had the V617F mutation, and all 5 V617F-negative patients (23%) had the exon 12 mutation. We found 3 different exon 12 mutations: 3 N542-E543del, 1 F537-K539de...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPK7KGOWPHYWM0
更新日期:2010-08-01 00:00:00
abstract::The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.3.343
更新日期:1978-09-01 00:00:00
abstract::To determine the performance and frequency of protein C reagents currently used by clinical laboratories, we analyzed North American Specialized Coagulation Laboratory Association (NASCOLA) protein C proficiency testing data from 6 surveys conducted in 2009 and 2010 (2009-1 to 2009-3 and 2010-1 to 2010-3). Interlabora...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP8MWU4QSTCLPU
更新日期:2012-06-01 00:00:00
abstract::Two cases of giant-cell arteritis of uterus were found incidentally in association with uterine prolapse. One similar case has been reported in the literature. Microscopic examination disclosed fragmentation of the elastic laminae of the myometrial arteries with a giant-cell and mononuclear infiltrate. The relationshi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.3.308
更新日期:1976-03-01 00:00:00
abstract:OBJECTIVES:Preanalytical factors, such as hemolysis, affect many components of a test panel. Machine learning can be used to recognize these patterns, alerting clinicians and laboratories to potentially erroneous results. In particular, machine learning might identify which cases of elevated potassium from a point-of-c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa039
更新日期:2020-07-07 00:00:00
abstract::The demonstration of carcinoembryonic antigen (CEA) in serum and in tissue sections may be of value in diagnosis and follow-up of several malignant tumors. Anti-CEA sera, however, cross-react with normal antigens, also present in tumors. The staining patterns of three commercially produced anti-CEA sera were investiga...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.1.25
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVES:Bladder cancers invading the muscularis mucosae (MM) are treated differently from those invading the muscularis propria (MP). However, it may be difficult to determine the type of smooth muscle in transurethral resection (TUR) or biopsy specimens. We aimed to investigate the clinicopathologic features of bla...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa030
更新日期:2020-07-07 00:00:00
abstract::Determination of breast cancer estrogen receptor (ER) status as a predictor of tumor response to adjuvant endocrine therapy remains a mainstay of breast cancer management. Recent second generation anti-ER antibodies and new epitope retrieval methods have produced paraffin-based immunohistochemical results that correla...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.3.332
更新日期:1996-09-01 00:00:00
abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.4.557
更新日期:1981-04-01 00:00:00
abstract:OBJECTIVES:Blood specimen hemolysis is a major cause of sample recollection in the neonatal intensive care unit. We aimed to reduce the hemolysis rate from 6.3% at baseline to less than 4% within the 9 months' duration of the study. METHODS:Intravenous infusion of lipid emulsion during sample collection, sample collec...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw086
更新日期:2016-07-01 00:00:00
abstract::A comparative evaluation of two commercial kits that detect antinuclear antibodies via indirect immunofluorescence was undertaken using an adult ambulatory population. The sensitivity and specificity of the kits were compared to determine which of the two was more efficient as a screening test for systemic lupus eryth...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.3.308
更新日期:1984-03-01 00:00:00
abstract::Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/7Y7N-M1WM-R0YK-M2VA
更新日期:2002-05-01 00:00:00
abstract::The examination of T-cell receptor (TCR) repertoires has an important role in the study of lymphoproliferative disorders and autoimmune diseases. Analysis of the complementarity-determining region 3 (CDR3) of the TCR beta chain is used to assess the clonality of T-cell populations. We developed a rapid fluorescence-ba...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/111.5.683
更新日期:1999-05-01 00:00:00
abstract::Reported is a case of a rare lymphoepithelial cyst of the tail of the pancreas that developed in a young man with no symptoms. To the authors' knowledge, it is the first case described to be entirely situated within the pancreas, thus confirming its pancreatic origin. Biologic behavior appears to be entirely benign, a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/98.2.188
更新日期:1992-08-01 00:00:00
abstract::The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polymarker (LDLR, GYPA, ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/KAWA-QHBU-6D1J-XY3G
更新日期:2002-09-01 00:00:00
abstract::The results of hepatitis B surface antigen (HBs-Ag) testing in a large volunteer blood donor population are described. Counterelectrophoresis and three versions of solid-phase radioimmunoassay technic are compared and evaluated. Initial results suggested that the radioimmunoassay technic are compared and evaluated. In...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/63.6.847
更新日期:1975-06-01 00:00:00
abstract::Previous studies suggest that low prostate weight is a significant negative prognostic factor for prostate cancer. In the current study, the data for 431 men who underwent radical retropubic prostatectomy between 1990 and 1998 were analyzed for association between prostate weight and various clinical and pathologic pa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPHGXDI94SGAC
更新日期:2010-04-01 00:00:00
abstract::A structure resembling a sulfur granule found in histologic sections of endometrium from patients with intrauterine contraceptive devices is described. It is distinguished from a true sulfur granule on morphologic grounds and is further characterized as a foreign substance, most probably fragments of the synthetic con...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.6.822
更新日期:1981-06-01 00:00:00
abstract::Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.3.512
更新日期:1976-09-01 00:00:00
abstract::The pattern of spurious red blood cell parameters caused by serum cold agglutinins has been well characterized for the Coulter counters but not the Ortho ELT-8. The pattern consists of a spurious increase in mean corpuscular volume (MCV), a discrepancy between the hemoglobin and the hematocrit, the latter falsely redu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.2.218
更新日期:1985-02-01 00:00:00