Abstract:
Objectives:Free light chain (FLC) measurement gained a lot of interest for diagnostic workup of monoclonal gammopathy. Methods:We evaluated the performance of turbidimetric polyclonal Freelite (The Binding Site, Birmingham, UK) assays on Cobas 6000 (Roche Diagnostics, Rotkreuz, Switzerland) and nephelometric monoclonal N Latex (Siemens Healthcare Diagnostics, Marburg, Germany) assays on BN ProSpec (Dade Behring, Deerfield, IL) vs established nephelometric Freelite assays on BN ProSpec. Results:Analytical performance was acceptable. Method comparison (n = 118) showed significant proportional FLC differences for N Latex assays. However, good correlation and clinical concordance were shown. Recovery study in the low concentration range demonstrated consistent over- and underrecovery for Freelite reagents, hampering future research on prognostic value of suppressed noninvolved FLC. Antigen excess detection was successful for κ FLC in three-fourths of cases with Freelite reagents and in all cases with N Latex reagents. However, the latter resulted in underestimated κ FLC concentrations. Conclusions:FLC analysis requires continuous awareness of analytical limitations. Monitoring of disease response requires FLC analysis on the same platform using the same reagents.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Messiaen AS,De Sloovere MMW,Claus PE,Vercammen M,Van Hoovels L,Heylen O,Debrabandere J,Vanpoucke H,De Smet Ddoi
10.1093/ajcp/aqx037subject
Has Abstractpub_date
2017-06-01 00:00:00pages
611-622issue
6eissn
0002-9173issn
1943-7722pii
3860304journal_volume
147pub_type
杂志文章abstract::The primary role of the surgical pathologist is to diagnose, and then to transmit this information effectively so that it can be used to guide patient management; therefore, the dual roles of the surgical pathologist as a diagnostician and consultant are inextricable. The diagnostic pathologist determines the nature, ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::Worldwide, infection with hepatitis viruses remains a major clinical and public heath concern. The laboratory is an integral part of the diagnosis of these infections since many of the manifestations are nonspecific, and the various hepatitis viruses show overlapping symptomatology. In this article, we discuss the lab...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/MAUW-9059-DQA6-RMRJ
更新日期:2002-12-01 00:00:00
abstract::We reviewed the cytologic features of 20 adrenal cortical carcinomas (ACCs; 9 primary and 11 metastatic) from 19 patients and highlighted diagnostic pitfalls. The mean size of primary ACCs was 11.9 cm, and that of metastatic ACCs was 3.0 cm. The metastatic sites were liver, lung, lymph node, soft tissue, and bone. Pri...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AQFEB5WEMU41N9K1
更新日期:2006-09-01 00:00:00
abstract::Fine-needle aspiration (FNA) of thyroid bed (TB) lesions is a common diagnostic modality in monitoring patients for recurrent cancer after a thyroidectomy. To elucidate the value of TB FNA, we reviewed our experience at The Johns Hopkins Hospital, Baltimore, MD. We identified 57 TB FNA specimens from 50 patients. Of t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPD60AHRUCMDPF
更新日期:2010-08-01 00:00:00
abstract::This study examines the relationship between age and mitogen-induced lymphocyte blastogenesis. Lymphocytes were stimulated with PHA; Concanavalin A (Con A), and PWM in 156 normal, healthy subjects ranging in age from birth to 75 years. The findings indicate a gradual but significant decrease in PHA- and Con A-induced ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.2.159
更新日期:1983-08-01 00:00:00
abstract:OBJECTIVES:To investigate the prognostic utility of multifocal extraprostatic extension (EPE) on biochemical recurrence after radical prostatectomy. METHODS:We conducted retrospective analysis of biochemical recurrence and prognostic pathologic variables in 673 men with stage pT3a/pT3b prostate cancer from 2000 to 201...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz193
更新日期:2020-03-09 00:00:00
abstract::Tumor cells in the cerebrospinal fluid of an American child with Burkitt's lymphoma are described. The morphology of the cells was demonstrated better by use of the "millipore" technic and hematoxylin and eosin staining than by the Wright-stained smear. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.2.238
更新日期:1975-08-01 00:00:00
abstract::The participant performances on the College of American Pathologists (CAP) Urine Survey specimens distributed during 1979 and 1980 for pregnancy testing are reviewed. Almost all the reagent methods used by the participants performed well on the negative specimens. Prominent differences in results on the positive speci...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::The "floral" variant of follicle center lymphoma (FCL) may be confused with progressive transformation of germinal centers or lymphocyte predominance Hodgkin lymphoma. Immunohistochemistry and gene rearrangement studies are usually sufficient to differentiate among these entities. We present 11 cases of floral FCL tha...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/V6PJ-BDAP-F0LU-CB6T
更新日期:2000-12-01 00:00:00
abstract::Management by Objectives (MBO) is results-oriented, participatory management, which is appropriate for the current era of keen competition for resources. MBO was installed in the author's institution in clinical laboratory divisions meeting prerequisites and fit the system to the local management environment. The resu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/86.6.738
更新日期:1986-12-01 00:00:00
abstract::Lesions of the breast containing extravasated mucin span a continuum from benign mucoceles to invasive mucinous (colloid) carcinoma. It is well known that distinguishing benign from malignant mucinous lesions is difficult infine-needle aspiration material. Whether these lesions also are difficult to distinguish in cor...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/TP6Y-Q0QR-XXVQ-9MJ5
更新日期:2002-07-01 00:00:00
abstract:Objectives:To examine the relationship between the recently defined Gleason grade groups and prostate cancer-specific mortality. Methods:If the probability of prostate cancer-specific death is symbolized as P(PSD), the probability of biochemical failure is symbolized as P(BF), and the probability of prostate cancer-sp...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw212
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVES:α1-Antitrypsin (AAT) deficiency is associated with an increased risk for lung and liver disease. Identification of AAT deficiency as the underlying cause of these diseases is important in correct patient management. METHODS:AAT deficiency is commonly diagnosed by demonstrating low concentrations of AAT foll...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPR7EIQS8PIMLV
更新日期:2014-05-01 00:00:00
abstract::The Digene Hybrid Capture 2 (hc2) high-risk human papillomavirus (HPV) DNA test (Digene, Gaithersburg, MD) is widely used for triage of women with atypical squamous cells of undetermined significance. Results in a "retest zone" (weakly positive tests) are repeated up to 2 times according to the Digene-recommended algo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4WCPTUV506HLP06P
更新日期:2007-01-01 00:00:00
abstract::The overall performances of several enzyme reagent kits for alkaline phosphatase, creatine kinase, lactic dehydrogenase, and aspartate aminotransferase were evaluated using an ABA-100 Bichromatic Analyzer. Interassay precision using this instrument with commercial reagents compared well with published data for similar...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/72.2.151
更新日期:1979-08-01 00:00:00
abstract::The role of fine-needle aspiration (FNA) in the diagnosis of breast carcinoma is established. We evaluated whether the degree of cellular dyscohesion and the nuclear grade in FNA material of breast carcinomas are reliable prognostic predictors for ipsilateral axillary lymph node metastasis. FNA specimens from 98 women...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/PR6K-7RXQ-NJUD-443Q
更新日期:2001-02-01 00:00:00
abstract:Objectives:Diagnosis of B-cell acute lymphoblastic leukemia (B-ALL) requires immunophenotypic evidence of B-lineage and absence of specific myeloid or T-lineage markers. Rare cases of otherwise typical B-ALL express myeloperoxidase (MPO) detectable by flow cytometry with an absence of other myeloid markers, but the cli...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqx021
更新日期:2017-04-01 00:00:00
abstract::Patients with chronic renal failure (CRF) are at increased risk for myocardial events that are difficult to evaluate due to atypical symptoms and chronically elevated protein markers of cardiac damage. This study evaluated cardiac troponin T (cTnT), a sensitive marker of cardiac injury, in patients with CRF without my...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.1.118
更新日期:1996-07-01 00:00:00
abstract::The expression of bcl-2, CD10, and CD20 was examined by multicolor flow cytometry in 78 samples including lymph node or other tissue biopsy specimens containing follicular lymphoma (FL; n = 17), reactive hyperplasia (RH; n = 28), or other malignant lymphomas (n = 20), as well as bone marrow aspirates (n = 13). The pre...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/820R-476K-Y9G0-3L9A
更新日期:2003-01-01 00:00:00
abstract::S-100 protein was detected in a small number of human peripheral T-lymphocytes by a direct immunoperoxidase method with the use of monospecific antibody to S-100 protein. Complemented-mediated lysis using monoclonal antibodies revealed that the S-100+ T-lymphocytes bore OKT3, OKT8, and OKT11 antigens but not OKT4, OKM...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.1.69
更新日期:1985-01-01 00:00:00
abstract::Campylobacter fetus subspecies jejuni has recently been recognized as a significant cause of enteritis in humans. The organism has been previously postulated to cause illness by direct invasion of the mucosa of the gastrointestinal tract, but this has not been documented. Reported is a case of Campylobacter enteritis ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.5.706
更新日期:1980-05-01 00:00:00
abstract::Differential characterization of the "reticulum cell" in lymphoreticular neoplasms. Am J Clin Pathol. 64: 171-179, 1975. The term "reticulum cell" is confusing, having been applied to the cells involved in many hematopoietic neoplasms, such as reticulum-cell sarcoma, histiocytic medullary reticulosis, leukemic reticul...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.2.171
更新日期:1975-08-01 00:00:00
abstract::Immunophenotypic analysis of acute leukemias is time consuming and often requires flow cytometric analysis. A 1-hour alkaline phosphatase-labeled streptavidin-biotin immunocytochemical procedure was evaluated as an alternative. Seventeen cases of acute leukemia, including 10 acute lymphocytic (ALL) and 7 acute nonlymp...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.6.876
更新日期:1992-06-01 00:00:00
abstract::The authors studied the effect of averaging replicated assays of Factor IX coagulation activity and Factor IX antigen on each plasma specimen in improving the ability to detect carriers of hemophilia B. The improvement resulting from this procedure is particularly marked in tests depending on the linear regression of ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/72.6.1000
更新日期:1979-12-01 00:00:00
abstract::Absolute lymphocytosis after nonsurgical trauma was investigated in three patient groups at an acute-care tertiary referral hospital. The first group, with mild-to-moderate trauma, consisted of 64 patients who survived knife wounds to the chest and abdomen. Thirteen of the 64 patients had admission lymphocyte counts g...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/86.4.480
更新日期:1986-10-01 00:00:00
abstract::Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.1.35
更新日期:1991-01-01 00:00:00
abstract::The amount of trapped plasma in the microhematocrit red blood cell column of samples from 25 normal individuals and 102 patients was investigated. The mean value for the normal individuals was 1.53%, and the mean values for the samples from the patient groups ranged from 1.41% to 1.82%. These groups included patients ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/78.5.770
更新日期:1982-11-01 00:00:00
abstract::Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.3.512
更新日期:1976-09-01 00:00:00
abstract::Two monoclonal antibodies, UCD/AB 6.11 and UCD/PR 10.11, were evaluated for their patterns of immunohistochemical reactivity in a survey of paraffin-embedded human tissues by the avidin-biotin-immunoperoxidase technique. By two-dimensional immunoblotting, UCD/AB 6.11 reacts with keratin number 18 and UCD/PR 10.11 iden...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/89.4.472
更新日期:1988-04-01 00:00:00
abstract::Forty-five renal biopsies with amyloidosis were studied by light microscopy with Congo red staining and action of potassium permanganate and by immunofluorescence with antihuman tissue A component antiserum antilight and heavy chains of immunoglobulins antisera. The patients were classified on the basis of concordance...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/87.6.756
更新日期:1987-06-01 00:00:00