Early use of indirect immunofluorescence for the detection of respiratory syncytial virus in HEp-2 cell culture.

Abstract:

:Respiratory syncytial virus is detected in cell culture by the presence of cytopathic effect. To detect RSV before cytopathic effect is usually seen, slides were evaluated retrospectively from 482 HEp-2 cell cultures on days 2-4 after inoculation. Indirect immunofluorescent staining detected RSV in 57 of 94 cultures that eventually were found positive by cytopathic effect. In an additional 19 cases that ultimately showed no cytopathic effect, RSV also was detected. In 15 of the latter cases, the presence of RSV was confirmed in the original specimen. Use of indirect immunofluorescence can be used to augment the sensitivity of cell culture for the detection of RSV because cytopathic effect may not always be evident.

journal_name

Am J Clin Pathol

authors

Bromberg K,Tannis G,Daidone B

doi

10.1093/ajcp/96.1.127

subject

Has Abstract

pub_date

1991-07-01 00:00:00

pages

127-9

issue

1

eissn

0002-9173

issn

1943-7722

journal_volume

96

pub_type

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