Abstract:
:A rapid, convenient immunologic assay technic for fibrinogen is reported. Basically a latex agglutination, the assay technic, called kinetic latex agglutinometry, quantitates the increase in light transmission that occurs in a stirred suspension of anti-fibrinogen-coated latex beads after the addition of a plasma or whole blood sample containing fibrinogen. In this system the time "delta t" required for a given transmission change to occur is inversely proportional in a log-log relationship to the quantity of fibrinogen in the plasma sample. The reaction is linear over a fibrinogen concentration of greater than 100-fold. The sensitivity can be adjusted over a wide range, and the assay can quantitate as little as 10 ng fibrinogen. The assay can be used with either plasma or whole blood. When compared to the thrombin clotting time method of Clauss, the correlation coefficient is 0.99 for the plasma assay and 0.95 for the whole blood assay. As a one-step assay employing stable reagents and requiring approximately two minutes per assay for normal plasma, the method is ideally suited for use in the clinical laboratory.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Babson AL,Opper CA,Crane LJdoi
10.1093/ajcp/77.4.424subject
Has Abstractpub_date
1982-04-01 00:00:00pages
424-9issue
4eissn
0002-9173issn
1943-7722journal_volume
77pub_type
杂志文章abstract::A 73-year-old man with myelodysplastic syndrome developed during the course of his illness auto-immune hemolytic anemia. The association between auto-immune hemolysis and myeloproliferative disease is extremely rare. An even more unique finding is the nature of the antibody which demonstrated single specificity agains...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.3.356
更新日期:1982-03-01 00:00:00
abstract::We evaluated a comprehensive deidentification engine at the University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, that uses a complex set of rules, dictionaries, pattern-matching algorithms, and the Unified Medical Language System to identify and replace identifying text in clinical reports while preserving ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/E6K3-3GBP-E5C2-7FYU
更新日期:2004-02-01 00:00:00
abstract::We compared the morphologic findings of different types of marginal zone B-cell lymphoma (MZL) involving the bone marrow (BM), including 18 splenic (SMZL), 6 extranodal (mucosa-associated lymphoid tissue lymphoma), and 6 nodal cases. The median percentage of BM involvement was 15%, and multiple overlapping patterns of...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/HRHQFBFTR8B4LXT4
更新日期:2008-05-01 00:00:00
abstract::Gastrointestinal stromal tumors (GISTs) are mesenchymal tumors characterized by mutations of KIT or PDGFRA. The objectives of this study were to evaluate BRAF mutations in GISTs and then to correlate BRAF mutational status in the tumor with clinical parameters, with B-raf expression, and with activation of some cellul...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPCKGA2QGBJ1R
更新日期:2010-01-01 00:00:00
abstract::Elevated T-cell leukemia-1 (TCL1) oncoprotein expression might promote human Burkitt lymphoma (BL) because increased TCL1 causes Burkitt-like lymphomas in TCL1 transgenic mice. Epstein-Barr virus (EBV) infection has been implicated as a cause of increased TCL1 expression in multiple BL cell lines, suggesting a critica...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/77V7U4E03V69QHRR
更新日期:2005-10-01 00:00:00
abstract::Hepatitis C virus (HCV) infection may be complicated by non-Hodgkin's lymphoma. We describe eight cases of B-cell extranodal non-Hodgkin's lymphoma occurring during the course of chronic HCV-related hepatic disease (low-grade of mucosa-associated lymphoid tissue [MALT]-type; diffuse large cell; Burkitt; diffuse small ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/109.5.600
更新日期:1998-05-01 00:00:00
abstract::The authors investigated assays for free protein S (ProS) antigen, total ProS antigen, and ProS crossed immunoelectrophoresis (CIEP) in the diagnosis of type 1 inherited ProS deficiency. Accurate measurement of the hemostatically important free ProS required showing that, on each specimen, precipitation of the C4b-bin...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.2.176
更新日期:1990-08-01 00:00:00
abstract::B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, includi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/29DA-CY9K-BHNW-4BG6
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVES:To investigate human epidermal growth factor receptor 2 (HER2)-positive nonpleomorphic invasive lobular carcinoma (ILC), which has rarely been addressed. METHODS:Clinicopathologic characteristics and follow-up of HER2-positive nonpleomorphic ILCs were collected and compared to those of HER2-negative counter...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz194
更新日期:2020-04-15 00:00:00
abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP42WMHSSTPHXI
更新日期:2015-03-01 00:00:00
abstract::We describe 2 male patients in whom hepatosplenic gamma/delta T-cell lymphoma (HSTL) developed 6 and 10 years after renal transplantation. The onset was abrupt with systemic symptoms, cytopenia, and hepatosplenomegaly. The histologic examination of the spleen (case 1), liver, and bone marrow revealed sinusoidal infilt...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/TC9U-FAV7-0QBW-6DFC
更新日期:2001-07-01 00:00:00
abstract:OBJECTIVES:To investigate the clinicopathologic features of extranodal natural killer/T-cell lymphoma (ENKTL) not preceded by another Epstein-Barr virus (EBV)-related disorder in children and adolescents. METHODS:A series of 17 cases of ENKTL not preceded by another EBV-related disorder in children and adolescents in ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqv010
更新日期:2016-01-01 00:00:00
abstract::The amount of trapped plasma in the microhematocrit red blood cell column of samples from 25 normal individuals and 102 patients was investigated. The mean value for the normal individuals was 1.53%, and the mean values for the samples from the patient groups ranged from 1.41% to 1.82%. These groups included patients ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/78.5.770
更新日期:1982-11-01 00:00:00
abstract::An atypical form of macrocytosis termed volumetric macrocytosis is described. In contrast to the macrocyte associated with megaloblastic anemia and the pseudomacrocyte caused by viscoelastic defects, the volumetric macrocyte is characterized by an increased mean corpuscular volume and a normal cell diameter. The volum...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.3.337
更新日期:1986-03-01 00:00:00
abstract::We previously reported 24 cases of marrow fibrin-ring granuloma (FRG) encountered in 1 institution and concluded that, contrary to previous studies showing marrow FRG as a diagnostic marker for Q fever, Epstein-Barr virus (EBV) was the most common proven cause of such FRG. The present study characterized patients with...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPB7SX7QXASPSK
更新日期:2010-02-01 00:00:00
abstract::Using Venn diagrams derived from set theory, a mathematic model is presented that allows triple-label immunophenotypic analysis of cells based upon double-label measurements. Practical applications of this method are illustrated and discussed. Using this model, triple-label data concerning Leu-8 and Leu-9 (CD7) antige...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/92.1.73
更新日期:1989-07-01 00:00:00
abstract::In a case of secondary syphilis with gastric involvement, the clinical history and roentgenographic and gastroscopic examiniations initially suggested an infiltrative malignant neoplasm. However, macroscopic and histologic examination of the partial gastrectomy specimen demonstrated an erosive, inflammatory lesion. Su...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/63.6.895
更新日期:1975-06-01 00:00:00
abstract::Three paired samples were distributed at three--six-month intervals in the Basic Ligand Survey during 1976. The stabilities of mean values obtained by widely used commercial kit procedures and by all methods for eight analytes are reported. Significant improvements in accuracy and long-term precision were identified i...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.1.35
更新日期:1991-01-01 00:00:00
abstract::Nineteen patients with acute nonlymphocytic leukemia were found to have a pericentric inversion of chromosome 16. All were classified as having acute myelomonocytic leukemia (M4) according to French-American-British Cooperative Group (FAB) criteria. Consistent abnormalities of marrow eosinophils were present. In 15 of...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.6.733
更新日期:1984-06-01 00:00:00
abstract:OBJECTIVES:Lymphoid enhancer binding factor 1 (LEF1) is expressed in most cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and has shown utility in distinguishing CLL/SLL from other small B-cell lymphomas. LEF1 expression has not been systematically studied in CD5-positive marginal zone lympho...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz207
更新日期:2020-04-15 00:00:00
abstract::The effects of physiologic temperature variations on antibiotic-induced killing of Escherichia coli, Pseudomonas aeruginosa, Streptococcus faecalis, and Streptococcus pneumoniae were studied. At concentrations less than or equal to four times the minimal bactericidal concentration, the activity of each of the antibiot...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/76.1.57
更新日期:1981-07-01 00:00:00
abstract::An automated fluorescent immunoassay system (FIAX) was compared with complement fixation (CF) for measuring antibodies to cytomegalovirus (CMV) and herpes simplex virus (HSV) in clinical specimens. Compared with CF the specificity of FIAX (ability to detect a negative titer) was 95% for CMV and 92% for HSV. The sensit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/76.3.305
更新日期:1981-09-01 00:00:00
abstract::Three cases of microcystic adenomas of the pancreas with special reference to multicentric origin are described. The gross features and light microscopic findings were consistent with those described as being microcystic adenomas, but in two cases the gross examination and gelatin-embedded giant slices revealed multip...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.2.150
更新日期:1990-08-01 00:00:00
abstract:OBJECTIVES:Bone marrows (BMs) with incidentally identified, small monotypic B-cell populations (MBPs) were evaluated. METHODS:BM aspirates with MBPs representing 5% or less of total events by flow cytometry, less than 5.0 × 10(9)/L B cells in blood, and no history of lymphoma or MBP with a different phenotype from pri...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPD5TOBDJU4GKO
更新日期:2014-05-01 00:00:00
abstract::The cytologic features of fine-needle aspiration specimens from 16 breast carcinomas that closely simulated benign lesions were analyzed and compared to smears from fibroadenomas and fibrocystic change. No combination of features was found that accurately separated all benign and malignant cases. Many nuclei with disc...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/98.2.155
更新日期:1992-08-01 00:00:00
abstract::Characteristically, mantle cell lymphoma (MCL) expresses surface immunoglobulin (sIg), CD19, CD20, and CD5 and lacks CD10 and CD23. Rare CD5-MCL variants have been described. This report describes a case of leukemic MCL with morphologically and immunophenotypically distinct classic MCL and blastoid-variant MCL (BV-MCL...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/UD2C-6JVP-WHXQ-Q217
更新日期:2004-07-01 00:00:00
abstract::Neutrophil function was determined in resting and phagocytic cells by an assay of hexose monophosphate shunt activity performed on small volumes of whole blood. This new micromethod clearly differentiated between zymosan-stimulated and unstimulated hexose monophosphate shunt activity. Furthermore, the whole blood micr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.1.88
更新日期:1980-07-01 00:00:00
abstract::Terminal deoxynucleotidyl transferase (TdT) is a marker of lymphoblastic disorders. Previously, the technique for determining TdT on smears was by indirect immunofluorescence. An immunoperoxidase procedure for detecting TdT on smears is reported. Results are in a comparable range with the indirect immunofluorescent te...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.2.174
更新日期:1982-02-01 00:00:00
abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.15
更新日期:1991-07-01 00:00:00