Abstract：
:Plasma biomarker analysis requires intact unbiased starting material. We analyzed the effects on plasma protein profiles of protease inhibitor cocktails and preprocessing storage. Plasma from 12 healthy subjects collected with and without protease inhibitors was prepared immediately and after 2 hours of room temperature storage. The samples were analyzed by a multiplexed enzyme-linked immunosorbent assay that captured 99 chemokines and cytokines. Unsupervised hierarchical analysis clustered the samples into 4 groups; one composed predominantly of samples processed immediately, another of samples processed after 2 hours, and the remaining two were a mix of immediate and 2-hour samples. The mixed and immediate group protein profiles were similar. However, among the immediate and 2-hour samples, the levels of 37 factors differed significantly: all were greater after 2 hours. The dramatic changes in protein levels during storage were independent of protease inhibitors and are likely due to cytokine production and/or release by leukocytes and platelets.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Ayache S,Panelli M,Marincola FM,Stroncek DFdoi
10.1309/3WM7-XJ7R-D8BC-LNKXsubject
Has Abstractpub_date
2006-08-01 00:00:00pages
174-84issue
2eissn
0002-9173issn
1943-7722pii
3WM7XJ7RD8BCLNKXjournal_volume
126pub_type
杂志文章abstract：:Fatty acid synthase is an enzyme that catalyzes the synthesis of long-chain fatty acids. The enzyme expression is minimal in adult tissues and very high in many cancers. Ulcerative colitis is a chronic inflammatory bowel disease that, when long-standing, is associated with an increased risk of colon cancer. The aim of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/pubvqndnvqkjvc8m
更新日期：2006-07-01 00:00:00
abstract：:Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/74.4.371
更新日期：1980-10-01 00:00:00
abstract：:The authors analyzed the frequency of immunophenotypic abnormalities in 1,474 cases of routinely fixed, paraffin-embedded B-lineage non-Hodgkin's lymphomas. B-lineage was determined by immunoreactivity for CD20 (L26, 92%); CD45RA (4KB5, an additional 3%) or immunoglobulin (Ig) light chain restriction (remaining 5%). C...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/102.6.825
更新日期：1994-12-01 00:00:00
abstract：:This study compared the ability to diagnose and accurately classify hepatic mass lesions by ultrasound-guided biopsy using two different needle types: a 20 gauge needle that yielded primarily cytologic material and an 18 gauge automated core biopsy needle. Slides prepared from each of the needle types were separately ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.5.583
更新日期：1995-11-01 00:00:00
abstract：:Antiphospholipid antibodies (aPLs) have been implicated in atherogenesis. We studied 344 patients with acute coronary syndromes; approximately 40% were aPL+ in 1 or more tests and 60% aPL-. In 215 patients, coronary artery disease (CAD) was angiographically documented, with 43.7% positive for aPL vs 34.9% of patients ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP2FJUT2YZGITK
更新日期：2009-10-01 00:00:00
abstract：:An atypical form of macrocytosis termed volumetric macrocytosis is described. In contrast to the macrocyte associated with megaloblastic anemia and the pseudomacrocyte caused by viscoelastic defects, the volumetric macrocyte is characterized by an increased mean corpuscular volume and a normal cell diameter. The volum...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.3.337
更新日期：1986-03-01 00:00:00
abstract：Objectives:Approximately 15% of breast cancers show equivocal human epidermal growth factor receptor 2 (HER2) results on HER2 immunohistochemistry (IHC) and are reflexed for fluorescence in situ hybridization (FISH). However, some cases remain equivocal. In this study, we evaluated these double-equivocal cases by using...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw211
更新日期：2017-01-01 00:00:00
abstract：:The results obtained with eight pregnancy kits on 735 urines were compared. Overall accuracies were similar and fairly high (93.4 to 95.37%), although only 78.28% of all pregnancies were simultaneously detected by all eight kits. It is suggested that the different performances of each kit could be accounted for not on...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.4.452
更新日期：1975-10-01 00:00:00
abstract：:Three activated partial thromboplastin time (APTT) reagent test systems, General Diagnostics Automated APTT, American Dade Actin FS, and Pacific Hemostasis (Thromboscreen KAPTT) reagent, containing different activators for the APTT assay, were evaluated for their precision and sensitivity to factor deficiencies in the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.1.43
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVES:High-grade serous carcinoma (HGSC) is the most common ovarian malignancy. The role of cytopathology in obtaining tissue diagnosis before institution of neoadjuvant chemotherapy (NACT) was evaluated. METHODS:All histopathology-proven HGSC specimens between 2015 and 2018 with prior cytopathologic diagnosis by...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa028
更新日期：2020-06-08 00:00:00
abstract：:We aimed to establish whether morphologic parameters were prognostically important in a large series of breast phyllodes tumors in Asian women. Of 335 phyllodes tumors diagnosed at the Department of Pathology, Singapore General Hospital, Singapore, between January 1992 and December 2002, 250 (74.6%) were benign, 54 (1...
journal_title：American journal of clinical pathology
pub_type： 历史文章,杂志文章
doi：10.1309/U6DV-BFM8-1MLJ-C1FN
更新日期：2005-04-01 00:00:00
abstract：:A multiplex polymerase chain reaction (PCR) assay using oligonucleotide primers to detect mecA and 16S ribosomal RNA gene was developed to aid in identification of methicillin-resistant staphylococci. Validation included 99 isolates of staphylococcus grouped into one of five categories: methicillin-susceptible coagula...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.3.368
更新日期：1997-03-01 00:00:00
abstract：:The cases of five patients who had sinus histiocytosis with massive lymphadenopathy accompanied by osseous involvement by the disease are presented. Four of the five patients were children. The bone lesions were multiple in four cases and solitary in one. The long bones were most commonly involved (three patients); ot...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.2.131
更新日期：1981-02-01 00:00:00
abstract：Objectives:To examine the relationship between the recently defined Gleason grade groups and prostate cancer-specific mortality. Methods:If the probability of prostate cancer-specific death is symbolized as P(PSD), the probability of biochemical failure is symbolized as P(BF), and the probability of prostate cancer-sp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw212
更新日期：2017-03-01 00:00:00
abstract：:Noncardiogenic pulmonary edema caused by transfusion has been observed for almost 60 years. Today, we know this entity as transfusion-related acute lung injury (TRALI). TRALI is an uncommon but potentially fatal adverse reaction to transfusion of plasma-containing blood components. It is typified by dyspnea, cough, hy...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/D3F7BXH466AE3G0P
更新日期：2008-02-01 00:00:00
abstract：:A case of a patient with otherwise typical multiple myeloma whose myeloma cells were phagocytic is described. Phagocytosis of erythrocytes, erythrocytic precursors, lymphocytes, and platelets was found. Ingestion of latex particles by myeloma cells was demonstrated in vitro. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.6.722
更新日期：1979-06-01 00:00:00
abstract：OBJECTIVES:The aim of this study is to determine if the diagnosis of nephrogenic adenoma (NA) can be made on cytologic criteria alone and if pair box gene transcription factor 8 (PAX8) is useful in the diagnosis of NA in daily cytology practice. METHODS:Cytologic features of NA previously described in a literature wer...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw006
更新日期：2016-03-01 00:00:00
abstract：:A structure resembling a sulfur granule found in histologic sections of endometrium from patients with intrauterine contraceptive devices is described. It is distinguished from a true sulfur granule on morphologic grounds and is further characterized as a foreign substance, most probably fragments of the synthetic con...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.6.822
更新日期：1981-06-01 00:00:00
abstract：:Two hundred forty-three patients who had pathologically verified primary osteosarcomas of bone, treated at The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute over a 24-year period, form the basis of this study. These cases provide a basis for comparison of those patients currentl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/67.5.427
更新日期：1977-05-01 00:00:00
abstract：:We compared prothrombin times (PTs) and international normalized ratios (INRs) for blood samples drawn into plastic vs glass collection tubes. We collected 60 venous blood samples into 4.5-mL glass and 2 plastic tubes (2.7 and 3.5 mL). An additional 153 samples, including 63 from warfarin-anticoagulated patients, were...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:The authors studied the performance of four measures of laboratory turnaround time: the mean, median, 90th percentile, and proportion of tests reported within a predetermined cut-off interval (proportion of acceptable tests [PAT]). Measures were examined with the use of turnaround time data from 11,070 stat partial th...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.4.452
更新日期：1989-04-01 00:00:00
abstract：:In a child with renal failure and oliguria due to hyperoxaluria myelophthisis developed as a result of extensive bone-marrow replacement with calcium oxalate crystals and an accompanying fibrous proliferations. The histopathology associated with this metabolic disorder was demonstrated in posterior iliac crest bone-ma...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/66.6.991
更新日期：1976-12-01 00:00:00
abstract：:The clinical, light microscopic, and immunohistochemical features of 14 sinonasal malignant melanomas were studied to show their diverse morphologic appearance and distinction from therapeutically more amenable neoplasms that occur in this region. The tumors arose in 6 men and 8 women (median age, 70 years). Eleven pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.6.689
更新日期：1991-12-01 00:00:00
abstract：:Our purpose was to evaluate the clinical, histologic, and histochemical staining characteristics of intestinal metaplasia (IM) at an endoscopically normal-appearing esophagogastric junction (IM-EGJ) compared with IM in a columnar-lined esophagus (IM-CLE). A prospective study included 253 patients referred for elective...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/N15U-FN5R-3M5D-PE0U
更新日期：2002-01-01 00:00:00
abstract：:In patients infected with HIV, high-grade B-cell non-Hodgkin's lymphomas (NHL), including the small noncleaved cell (SNCC) category, exhibit pleomorphic features, which makes precise definition difficult. Sixty-nine pathologic specimens with HIV-related systemic lymphomas, including 42 SNCC, 20 immunoblastic lymphomas...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.5.561
更新日期：1995-05-01 00:00:00
abstract：:The case of a 23-year-old woman with a primary small cell carcinoma of the endometrium is reported. The light microscopic features were similar to those of small cell carcinoma of the lung and those of the uterine cervix. Electron microscopy revealed cytoplasmic neurosecretory type granules and indistinct cell junctio...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.1.98
更新日期：1984-01-01 00:00:00
abstract：:Carumonam, a new monobactam, was found to have an anti-microbial spectrum similar to aztreonam. Its spectrum includes Enterobacteriaceae, Haemophilus influenzae, pathogenic Neisseria species, Pseudomonas aeruginosa, and some streptococci. Staphylococcus species, enterococci, and many other nonenteric gram-negative bac...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.5.608
更新日期：1986-11-01 00:00:00
abstract：:A 55-year-old man presented with nerve compression and examination of tissue removed by laminectomy, and bone marrow aspiration was diagnostic of multiple myeloma. Protein studies showed a total serum protein of 5.7 g/dL, with a M-component in the fast beta region. The abnormal protein reacted only with anti-lambda an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Serum creatine kinase (CK) was measured in 515 healthy white women and 28 obligate carriers for Duchenne muscular dystrophy. There was substantial overlap between the control and carrier populations. To analyze the impact of the degree of overlap, the predictive value of a CK result was determined by (1) using sensiti...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.5.655
更新日期：1985-11-01 00:00:00
abstract：:The immunochemical characterization of the light chains of the mesangial immunoglobulin A (IgA) deposits were studied in 45 patients with IgA nephropathy. Kappa and lambda light chains were detected with direct immunofluorescence (IF) method, using monospecific rabbit anti-human anti-kappa and anti-lambda anti-sera. T...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.5.548
更新日期：1986-05-01 00:00:00
