Abstract:
:A multiplex polymerase chain reaction (PCR) assay using oligonucleotide primers to detect mecA and 16S ribosomal RNA gene was developed to aid in identification of methicillin-resistant staphylococci. Validation included 99 isolates of staphylococcus grouped into one of five categories: methicillin-susceptible coagulase-negative staphylococcus (MSCNS), methicillin-resistant coagulase-negative staphylococcus (MRCNS), methicillin-susceptible Staphylococcus aureus (MSSA), high beta-lactamase producing S aureus (HiBSA), and methicillin-resistant S aureus (MRSA). mecA was detected in MRSA (21/21), and in MRCNS (20/20), but not in MSSA (0/20). mecA was occasionally detected in HiBSA (1/19) and MSCNS (3/19). This multiplex PCR assay was also used to test 30 clinical isolates of coagulase-negative staphylococci with discrepancies between results of in vitro tests for susceptibility to oxacillin and was found to be valuable when a more definitive determination of intrinsic methicillin-resistance was desired.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Salisbury SM,Sabatini LM,Spiegel CAdoi
10.1093/ajcp/107.3.368subject
Has Abstractpub_date
1997-03-01 00:00:00pages
368-73issue
3eissn
0002-9173issn
1943-7722journal_volume
107pub_type
杂志文章abstract::Fresh frozen plasma (FFP) normally requires about 45 min to thaw in a 37 degrees C water bath when placed inside an additional plastic overbag. That relatively prolonged time may result in non-utilization or delays in delivery of the product, especially, during emergency surgery. One report recommends the use of a mic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/78.2.220
更新日期:1982-08-01 00:00:00
abstract::We examined the occurrence of JAK2(V617F) and JAK2 exon 12 mutations in a clinical cohort of polycythemia vera (PV) in Taiwan. Of 22 patients with PV, 17 (77%) had the V617F mutation, and all 5 V617F-negative patients (23%) had the exon 12 mutation. We found 3 different exon 12 mutations: 3 N542-E543del, 1 F537-K539de...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPK7KGOWPHYWM0
更新日期:2010-08-01 00:00:00
abstract::The Society for Hematopathology and European Association for Haematopathology workshop, from October 27 to 29, 2011, in Los Angeles, CA, exhibited many exemplary skin biopsy specimens with interesting inflammatory changes mimicking features of cutaneous lymphoma. This article reviews features observed in cutaneous lym...
journal_title:American journal of clinical pathology
pub_type: 共识发展会议,杂志文章
doi:10.1309/AJCPX4BXTP2QBRKO
更新日期:2013-04-01 00:00:00
abstract::Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.3.512
更新日期:1976-09-01 00:00:00
abstract::Giant lymph node hyperplasia is uncommon in children and is exceptionally rare in infancy. The authors report a case of giant lymph node hyperplasia that presented as a right supraclavicular mass in an asymptomatic two-month-old female infant. Clinically, the mass was thought to be either a lipoma or a cystic hygroma,...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.3.344
更新日期:1989-03-01 00:00:00
abstract::Management by Objectives (MBO) is results-oriented, participatory management, which is appropriate for the current era of keen competition for resources. MBO was installed in the author's institution in clinical laboratory divisions meeting prerequisites and fit the system to the local management environment. The resu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/86.6.738
更新日期:1986-12-01 00:00:00
abstract:OBJECTIVES:To evaluate the impact of implementing the dual interpretation of atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and low-grade squamous intraepithelial lesion (LSIL) after the Bethesda System 2014 and to compare it with other indeterminate interpretations. METHODS...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa069
更新日期:2020-09-08 00:00:00
abstract::Centerin (SERPINA9/GCET1) is a protease inhibitor with expression restricted to germinal center B cells and lymphoid malignancies with germinal center B-cell maturation. Expression of the centerin gene transcript, along with bcl-6 and GCET2/HGAL, constitutes a molecular signature associated with a good prognosis in di...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/9QKE68QU7B825A3U
更新日期:2008-07-01 00:00:00
abstract::The ATHENA (Addressing THE Need for Advanced HPV Diagnostics) HPV study evaluated the clinical usefulness of the cobas HPV Test (Roche Molecular Systems, Pleasanton, CA) for high-risk human papillomavirus (HR-HPV) testing (14 HR types) and individual HPV-16/HPV-18 genotyping in women undergoing routine cervical cytolo...
journal_title:American journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1309/AJCPTUS5EXAS6DKZ
更新日期:2011-10-01 00:00:00
abstract::To our knowledge, there are no universally accepted, evidence-based guidelines for how to resolve the HER2 status of tumors demonstrating equivocal amplification. The present study was based on 17 breast core biopsy specimens demonstrating invasive carcinoma with equivocal HER2 amplification, defined as an HER2/chromo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/KFKDNK8CENVN24VU
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to determine if the diagnosis of nephrogenic adenoma (NA) can be made on cytologic criteria alone and if pair box gene transcription factor 8 (PAX8) is useful in the diagnosis of NA in daily cytology practice. METHODS:Cytologic features of NA previously described in a literature wer...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw006
更新日期:2016-03-01 00:00:00
abstract::Because T-regulatory cells (Tregs) can harmfully impair HIV-specific responses or beneficially limit immune activation, we compared the number of Tregs in lymph nodes from 48 HIV+ patients and 106 HIV- subjects. By using a microscope counting grid, we found that the mean ± SD number of Tregs in lymph nodes was 3 times...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPAR39BFWNHWRO
更新日期:2011-07-01 00:00:00
abstract::To evaluate the diagnostic utility of flow cytometry (FCM) as compared to cytology, DNA and RNA content of cells were measured in 233 samples of cerebrospinal fluid (CSF) from 147 patients with solid tumors or lymphomas and 17 controls with nonmalignant disorders. All control specimens were normal. Twenty-eight sample...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.5.570
更新日期:1987-11-01 00:00:00
abstract::Three cases of microcystic adenomas of the pancreas with special reference to multicentric origin are described. The gross features and light microscopic findings were consistent with those described as being microcystic adenomas, but in two cases the gross examination and gelatin-embedded giant slices revealed multip...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.2.150
更新日期:1990-08-01 00:00:00
abstract::Current methods for testing stool samples for hemoglobin utilize peroxidase oxidation of chemical indicators such as guaiac or benzidine. These tests have frequent false-positive and false-negative results, complicating random screening for occult gastrointestinal bleeding. The authors have developed an immunochemical...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/69.1.342
更新日期:1978-03-01 00:00:00
abstract:OBJECTIVES:Mesothelin (MSLN) is a differentiation antigen found to be overexpressed in intraductal papillary mucinous neoplasms (IPMNs) and is a potential treatment target in pancreatic ductal adenocarcinoma. METHODS:From institutional archives, 114 cases of resected pancreatic mucinous cysts were identified, includin...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPDTTL2I5ECMFG
更新日期:2014-09-01 00:00:00
abstract::S-100 protein was detected in a small number of human peripheral T-lymphocytes by a direct immunoperoxidase method with the use of monospecific antibody to S-100 protein. Complemented-mediated lysis using monoclonal antibodies revealed that the S-100+ T-lymphocytes bore OKT3, OKT8, and OKT11 antigens but not OKT4, OKM...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.1.69
更新日期:1985-01-01 00:00:00
abstract::Two patients with sporadic primary hyperparathyroidism were found to harbor tiny medullary thyroid carcinomas. In addition, parafollicular-cell hyperplasia was recognized in one of these thyroids by the use of immunoperoxidase stain to localize calcitonin. The possible relationships between these lesions are discussed...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/71.5.595
更新日期:1979-05-01 00:00:00
abstract::Wild-type apolipoprotein A-I (apo A-I)-derived amyloid commonly occurs in atherosclerotic plaques. To clarify apo A-I amyloid formation, plasma levels of apo A-I and cholesterol were related to the presence of amyloid in atherosclerotic plaques in 15 patients with peripheral atherosclerosis, subjected to arterial reco...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/PJE6-X9E5-LX6K-NELY
更新日期:2001-02-01 00:00:00
abstract::Workload recording as designed by the College of American Pathologists has proven to be an invaluable aid in the assessment of overall laboratory operation. The administrative information derived from the compilation of these workload statistics is utilized to provide effective and these workload statistics is utilize...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.4.469
更新日期:1980-10-01 00:00:00
abstract::Specific radioimmunoassays, sensitive to plasma levels of less than 1% of normal, were developed for protein C and Factor X. In 31 normal subjects, mean plasma antigen levels were as follows: protein C, 3.23 +/- 0.79 microgram/mL (2 SD); Factor X, 7.74 +/- 1.81 microgram/mL. In patient son chronic warfarin therapy, pr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.5.573
更新日期:1984-11-01 00:00:00
abstract::The efficacy of a noninvasive hemoglobin monitoring device (Astrin, Sysmex, Kobe, Japan) was evaluated for healthy volunteers and for patients with hematologic disorders. At the same time, the effects of WBC counts on noninvasive monitoring were studied by clinical evaluation and in ex vivo experiments. The hemoglobin...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/61AN-J1XV-3MW8-78YP
更新日期:2004-01-01 00:00:00
abstract::A monoclonal antibody prepared against estrogen receptor has been shown to be specific and sensitive for the detection of estrogen receptor in human breast lesions by use of immunohistochemical methods. Two hundred selected cases of primary breast carcinoma were assayed for estrogen receptor content by biochemical and...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/90.3.233
更新日期:1988-09-01 00:00:00
abstract::The Leu-8 antigen is found on the surface of many hematologic cells, including many T- and B-lymphocytes. With the use of a frozen-section immunoperoxidase technic, 152 B-cell non-Hodgkin's lymphomas were examined for Leu-8 expression. Of these lymphomas, 53% expressed Leu-8. Subclassification of the lymphomas with th...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.4.486
更新日期:1987-10-01 00:00:00
abstract::We describe 2 male patients in whom hepatosplenic gamma/delta T-cell lymphoma (HSTL) developed 6 and 10 years after renal transplantation. The onset was abrupt with systemic symptoms, cytopenia, and hepatosplenomegaly. The histologic examination of the spleen (case 1), liver, and bone marrow revealed sinusoidal infilt...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/TC9U-FAV7-0QBW-6DFC
更新日期:2001-07-01 00:00:00
abstract::This study was undertaken to evaluate the precision and sensitivity of three different commercial APTT reagents containing the activators kaolin, micronized silica, or ellagic acid. These reagents varied greatly in their ability to detect mild coagulopathies. The ellagic acid reagent was able to detect the mildest def...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.4.436
更新日期:1984-10-01 00:00:00
abstract::Ewing family tumors (EFTs) and prostate carcinomas are characterized by rearrangement of ETS genes, most commonly FLI1 (EFTs) and ERG (prostate carcinomas). Previously, we characterized an antibody against ERG (EPR3864) for detecting ERG-rearranged prostate carcinoma. Because EPR3864 also cross-reacts with FLI1, we ev...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPN4L1BMRQPEIT
更新日期:2013-06-01 00:00:00
abstract::Serum ferritin, serum iron, and unsaturated iron binding capacity were studied in 64 patients with beta homozygous thalassemia (BHT), 120 patients with beta heterozygous thalassemia, and 46 normal subjects. Incidence of iron overload seen in 32 BHT cases was similar in untransfused and transfused cases. Among heterozy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.1.103
更新日期:1985-07-01 00:00:00
abstract::Noncardiogenic pulmonary edema caused by transfusion has been observed for almost 60 years. Today, we know this entity as transfusion-related acute lung injury (TRALI). TRALI is an uncommon but potentially fatal adverse reaction to transfusion of plasma-containing blood components. It is typified by dyspnea, cough, hy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/D3F7BXH466AE3G0P
更新日期:2008-02-01 00:00:00
abstract::In today's environment, many laboratories and pathology practices are challenged to maintain or increase their quality while simultaneously lowering their overall costs. The cost of improving specimen processes is related to quality, and we demonstrate that actual costs can be reduced by designing "quality at the sour...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCPSMQYAF6X1HUT
更新日期:2012-09-01 00:00:00