Abstract：
:We examined the occurrence of JAK2(V617F) and JAK2 exon 12 mutations in a clinical cohort of polycythemia vera (PV) in Taiwan. Of 22 patients with PV, 17 (77%) had the V617F mutation, and all 5 V617F-negative patients (23%) had the exon 12 mutation. We found 3 different exon 12 mutations: 3 N542-E543del, 1 F537-K539delinsL, and 1 novel mutation, I540-E543delinsKK. Patients with V617F showed significantly higher WBC and platelet counts at diagnosis than patients with exon 12 mutations (P = .021 and P = .038, respectively). We report a surprisingly high incidence of exon 12 mutations in Taiwanese patients with PV, a result quite different from reports in the Western literature (P = .001). Our data suggest that exon 12 mutation of JAK2 in patients with PV may have an uneven geographic distribution. A clinical laboratory providing the V617F test alone may risk missing a substantial number of patients with PV in areas with a high incidence of exon 12 mutation.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Yeh YM,Chen YL,Cheng HY,Su WC,Chow NH,Chen TY,Ho CLdoi
10.1309/AJCPK7KGOWPHYWM0subject
Has Abstractpub_date
2010-08-01 00:00:00pages
266-70issue
2eissn
0002-9173issn
1943-7722pii
134/2/266journal_volume
134pub_type
杂志文章abstract：:A murine monoclonal antibody (MAb 12C3) that is specific to human ovarian carcinomas was generated by immunizing mice with a human ovarian germinoma cell line (JOHYC-2). The antigen distribution that was defined by MAb 12C3 in normal and malignant human tissues was analyzed by immunohistochemistry on paraffin-embedded...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.3.288
更新日期：1995-03-01 00:00:00
abstract：:Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP m...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.2.237
更新日期：1984-02-01 00:00:00
abstract：:A case of metastatic ameloblastoma of the maxilla with secondary hypercalcemia in a 54-year-old man is presented. After treatment with surgery, chemotherapy, and radiotherapy, the patient was found to have multiple metastases and severe hypercalcemia associated with high levels of parathormone-like substance in the pe...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.4.585
更新日期：1981-04-01 00:00:00
abstract：:A patient with prolymphocytic leukemia was immunophenotyped by whole blood and Ficoll-Hypaque methods. Discordance was noted between the two methods, especially for the HLE1, B1, I2, and T1B monoclonal markers. The disparity in results reflected the use of 15 X 10(6) cells versus 1 X 10(6) cells per reaction tube. Thi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.2.227
更新日期：1989-02-01 00:00:00
abstract：:The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.4.462
更新日期：1982-10-01 00:00:00
abstract：:From November 1985 to December 1990, 2,552 endomyocardial biopsy specimens from 209 heart transplant patients were studied. Forty-four (21%) patients developed 45 episodes of major human cytomegalovirus infection (HCMV). Human cytomegalovirus infection was primary in 13 of 44 patients. Thirty-one patients developed ep...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：OBJECTIVES:α1-Antitrypsin (AAT) deficiency is associated with an increased risk for lung and liver disease. Identification of AAT deficiency as the underlying cause of these diseases is important in correct patient management. METHODS:AAT deficiency is commonly diagnosed by demonstrating low concentrations of AAT foll...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPR7EIQS8PIMLV
更新日期：2014-05-01 00:00:00
abstract：:The demonstration of tartrate-resistant acid phosphatase (TRAP) activity has long been a cornerstone in the diagnosis of hairy cell leukemia (HCL). Recently a monoclonal antibody to this enzyme has been developed that can be used in an immunoperoxidase method on paraffin-embedded tissues. By using a peroxidase-labeled...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/108.3.308
更新日期：1997-09-01 00:00:00
abstract：:Four cases of acute myelofibrosis are contrasted to three cases of acute megakaryocytic leukemia. The cases were clinically indistinguishable. Light microscopic examination of hematoxylin and eosin-stained bone marrow biopsy specimens from the cases of acute myelofibrosis showed a trilinear proliferation of mature and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/88.5.578
更新日期：1987-11-01 00:00:00
abstract：:Fine-needle aspiration (FNA) is the standard of care for the initial workup of thyroid nodules, but there is no consensus algorithm to manage patients with benign results. We examined performance characteristics of initial and repeat satisfactory FNAs for all 402 patients who underwent thyroid surgery during a recent ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/4AXL-DMN1-JRPM-TX5P
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVES:The incidence of lymphoproliferative disorders (LPDs) is increasing in sub-Saharan Africa (SSA) due to population growth, aging, and human immunodeficiency virus (HIV). Despite significant burden, resources for diagnosis and treatment of LPDs are limited, with little infrastructure to deliver modern patholog...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw118
更新日期：2016-10-01 00:00:00
abstract：:Same-day turnaround of pathology specimens is desirable in this era of managed care, and rapid microwave tissue processing produces histologic features of a quality equivalent to overnight processing. We studied whether microwave-assisted rapid tissue processing adversely affects the quality of immunohistochemical sta...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/GN6Q-CBML-LEAT-LK2M
更新日期：2006-02-01 00:00:00
abstract：:A monoclonal antibody prepared against estrogen receptor has been shown to be specific and sensitive for the detection of estrogen receptor in human breast lesions by use of immunohistochemical methods. Two hundred selected cases of primary breast carcinoma were assayed for estrogen receptor content by biochemical and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.3.233
更新日期：1988-09-01 00:00:00
abstract：:A series of 127 renal specimens reflecting a wide spectrum of renal pathology was evaluated with a direct immunoperoxidase technic in which frozen tissue was first reacted with anti-Ig(G,A,M) and anti-C3-peroxidase conjugates and subsequently counterstained with periodic acid-Schiff-hematoxylin (IMPAS). This technic p...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.2.240
更新日期：1980-02-01 00:00:00
abstract：:Fine-needle cytology (FNC) of 292 palpable orbital and eyelid tumors was performed with a 25-gauge (0.5-mm), 3-cm needle and compared with the histopathologic findings in 286 cases. Among these 286 cases, a concordant diagnosis of malignancy and type was achieved in 249 cases (87%). False positive diagnoses were made ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.1.100
更新日期：1990-01-01 00:00:00
abstract：:Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.4.517
更新日期：1991-04-01 00:00:00
abstract：:An atypical form of macrocytosis termed volumetric macrocytosis is described. In contrast to the macrocyte associated with megaloblastic anemia and the pseudomacrocyte caused by viscoelastic defects, the volumetric macrocyte is characterized by an increased mean corpuscular volume and a normal cell diameter. The volum...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.3.337
更新日期：1986-03-01 00:00:00
abstract：:We evaluated a comprehensive deidentification engine at the University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, that uses a complex set of rules, dictionaries, pattern-matching algorithms, and the Unified Medical Language System to identify and replace identifying text in clinical reports while preserving ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/E6K3-3GBP-E5C2-7FYU
更新日期：2004-02-01 00:00:00
abstract：:Considerable progress has been achieved in our knowledge of the function of the chemokine system and in understanding its role in the pathophysiology of human diseases. This complex system, presently including approximately 50 cytokines and 20 receptors, coordinates leukocyte recruitment in a variety of human diseases...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/M6U4B8L6TNAK4G9L
更新日期：2005-06-01 00:00:00
abstract：:Cytokeratin (CK) expression was studied in 22 sinonasal inverted papillomas. Columnar (respiratory) epithelium in inverted papillomas abundantly expressed CK7, CK8, CK18, and CK19. Immunoreactivity for CK5/14 and CK17 was found in basal and parabasal/suprabasal cells. Transitional (cuboidal) and squamous epithelium in...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/TH5J-T1Q6-9QJR-8CDK
更新日期：2001-05-01 00:00:00
abstract：:The finding of monoclonal B-cell lymphocytosis (MBL) raises questions on the nature of clonal cell expansion and its risk of progression. We identified and characterized 7 cases of clinically benign clonal B-cell lymphocytosis. The clonal lymphocytes were clearly of CD5- and non-chronic lymphocytic leukemia (CLL) phen...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/WB56ET6AGM44TM5T
更新日期：2007-08-01 00:00:00
abstract：OBJECTIVES:Nucleic acid amplification tests on formalin-fixed, paraffin-embedded (FFPE) tissue specimens enable Mycobacterium tuberculosis complex (MTB) detection and rapid tuberculosis diagnosis in the absence of microbiologic culture tests. We aimed to evaluate the efficacy of different polymerase chain reaction (PCR...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP2QZRH4ZNPRDD
更新日期：2014-09-01 00:00:00
abstract：:Examination of kidneys of ten patients with uremia and severe hypertension treated by chronic intermittent hemodialysis revealed a deposition of glycogen within interstitial cells of the renal medulla. This is unlike any described renal distribution of glycogen. Electron microscopic studies performed in one case demon...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.3.400
更新日期：1979-09-01 00:00:00
abstract：:The Laboratory Proficiency Testing Program has been in effect for 20 years. During the past 6 years, samples were distributed for screening, investigation, and identification of hemoglobinopathies to test laboratory proficiency. Six samples for hemoglobin (Hb) S screening were distributed to from 37 to 163 laboratorie...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.5.567
更新日期：1997-05-01 00:00:00
abstract：:We optimized fluorogenic thrombin-generation assays with regard to sample volume, calibration, analytic corrections, and activation reagents. Lower sample volumes (40 vs 80 microL) were associated with better recovery of thrombin activity, lower interference due to absorbance of light, and higher total thrombin genera...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP6AY4HTRAAJFQ
更新日期：2009-08-01 00:00:00
abstract：:A unique case of intramural uterine tumor composed of malignant epithelium and benign smooth muscle, adipose tissue, and cartilage is reported. This tumor, which the authors designated "carcinomesenchymoma," apparently represents an example of a previously unreported variant of mixed müllerian tumor that is composed o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.5.746
更新日期：1981-05-01 00:00:00
abstract：:Fifteen cases of low-grade B-cell lymphoma involving unusual extranodal sites have been studied in comparison to cases reported or observed arising in typical mucosa-associated lymphoid tissues. In every case, histopathologic features conformed to those characteristic for lymphomas of mucosa-associated lymphoid tissue...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.6.738
更新日期：1991-12-01 00:00:00
abstract：:Data from 2.5 million glucose analyses on pools of lyophilized human quality control serum were used to evaluate analyte stability in the prereconstitution phase. Input information was from laboratories in Regional Quality Control Programs that use CAP Quality Assurance Service (QAS) data processing. Of 31 pools in us...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:An experimental computerized interactive morphometry system for the classification of lymphoid-rich effusions is presented. The relatively inexpensive microcomputer-based system was assembled in our laboratory with commercially available hardware and software. One hundred twenty-two effusions (86 benign lymphocytoses,...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.5.570
更新日期：1993-05-01 00:00:00
abstract：:In a computerized case registry, containing 220 cases of sinus histiocytosis with massive lymphadenopathy (SHML), 23 patients were identified with clinical or routine laboratory findings suggestive or diagnostic of immune dysfunction. We divided the abnormalities into hematologic autoantibodies (nine patients), glomer...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/82.5.515
更新日期：1984-11-01 00:00:00
