Abstract:
:Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique with the use of the following MoAbs: leukocyte common antigen (CD45), Mac 387, L26, 4KB5, MB1, MB2, LN2, UCHL1, MT1, and MT2. The lineage of the neoplastic cells was determined in all cases by gene rearrangement studies for immunoglobulin heavy chain and for the T-cell receptor beta-chain. Genotypic results showed B-cell lineage in 33 cases (75%), T-cell lineage in 6 cases (14%), and mixed or undetermined lineage in 5 cases (11%). A concordance of lineage assignment by paraffin section immunophenotyping with gene rearrangement studies was observed in 37 of 39 (95%) lymphomas with an unequivocally defined genotype. MoAb L26 was the most sensitive in detecting B-cell genotype; MoAbs MT1 and UCHL1 were the most sensitive and specific, respectively, in detecting T-cell genotype. The authors conclude that lineage assignment of NHLs in paraffin sections is reflective of the corresponding genotype when an appropriate panel of MoAbs is used.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Elghetany MT,Kurec AS,Schuehler K,Forbes BA,Duggan DB,Davey FRdoi
10.1093/ajcp/95.4.517subject
Has Abstractpub_date
1991-04-01 00:00:00pages
517-25issue
4eissn
0002-9173issn
1943-7722journal_volume
95pub_type
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doi:
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更新日期:1975-11-01 00:00:00
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更新日期:1992-06-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2010-11-01 00:00:00
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