Incidental findings of variant hemoglobin during hemoglobin A(1c) testing.

Abstract:

:A variant hemoglobin fraction may be an incidental finding during HbA(1c) analysis using the G8 Tosoh HPLC analyzer, but it is unclear if the retention times and fraction patterns can reliably predict the findings of a high-performance liquid chromatography (HPLC) β-thalassemia program (Bio Rad Variant II analyzer). We chose 100 samples sent for HbA(1c) determinations (G8 Tosoh) with an incidental finding of variant hemoglobin and did a reflex test using the Bio Rad Variant II analyzer (β-thalassemia program). Two observers attempted to predict the results with that analyzer from fraction patterns and retention times of the hemoglobin variants detected with the G8. They independently identified all hemoglobin variants (HbS, Hb Setif, HbC, and HbD) by their patterns and retention times. We conclude that HPLC confirmation of certain variant hemoglobin fractions found incidentally during HbA(1c) testing on the G8 Tosoh is not necessary.

journal_name

Am J Clin Pathol

authors

Froom P,Henig C,Zalman L,Barak M

doi

10.1309/AJCPJ0GSRWF0UBYT

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

425-8

issue

3

eissn

0002-9173

issn

1943-7722

pii

138/3/425

journal_volume

138

pub_type

杂志文章
  • Distribution of LDH-1 in normal, ischemic, and necrotic myocardium. An immunoperoxidase study.

    abstract::To study the distribution of lactate dehydrogenase (LDH-1) (H4) in normal, ischemic, and necrotic myocardium using the peroxidase-antiperoxidase technic, the authors studied formalin-fixed paraffin-embedded sections of human (n = 11) and canine (n = 28) myocardium. All normal control myocardium showed positive immunos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.2.198

    authors: Herscher LL,Siegel RJ,Said JW,Edwalds GM,Moran MM,Fishbein MC

    更新日期:1984-02-01 00:00:00

  • Analysis of human T-cell lymphotropic virus in CD25+ anaplastic large cell lymphoma in children.

    abstract::Anaplastic large cell lymphoma (ALCL) is recognized as 2 distinct diseases: anaplastic lymphoma kinase (ALK)+ ALCL and ALK- ALCL. ALK+ ALCL occurs in younger patients and has a better prognosis. Human T-cell lymphotropic virus (HTLV-1) is linked to the development of adult T-cell leukemia/lymphoma (ATLL), which freque...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP6Q7QMUVGMVMF

    authors: Gualco G,Chioato L,Weiss LM,Harrington WJ Jr,Bacchi CE

    更新日期:2009-07-01 00:00:00

  • The relationship between MIB-1 proliferation index and outcome in pancreatic neuroendocrine tumors.

    abstract::The expression of the cell-cycle-associated Ki-67 antigen by MIB-1 monoclonal antibody was retrospectively assessed in 35 surgically resected neuroendocrine tumor specimens of the pancreas embedded in paraffin. The MIB-1 proliferation index was correlated with the classification of the neuroendocrine tumors of the pan...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/109.3.286

    authors: Gentil Perret A,Mosnier JF,Buono JP,Berthelot P,Chipponi J,Balique JG,Cuilleret J,Dechelotte P,Boucheron S

    更新日期:1998-03-01 00:00:00

  • Pathology consultation on anticoagulation monitoring: factor X-related assays.

    abstract:OBJECTIVES:To review various anticoagulation therapies and related laboratory monitoring issues, with a focus on factor X-related chromogenic assays. METHODS:A case-based approach is used to review pertinent published literatures and product inserts of anticoagulation drugs and to look back on clinical use of factor X...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/AJCPR3JTOK7NKDBJ

    authors: Wool GD,Lu CM,Education Committee of the Academy of Clinical Laboratory Physicians and Scientists.

    更新日期:2013-11-01 00:00:00

  • True histiocytic lymphoma of the thymus. Report of a case and a study of the distribution of histiocytic cells in the fetal and adult thymus.

    abstract::The thymus was the site of a true histiocytic lymphoma. Immunohistochemical demonstration of lysozyme and alpha-1-antitrypsin in the tumor cells indicated the histiocytic nature of the tumor. Five fetal and five adult thymus glands were studied for the presence and distribution of true histiocytic cells. Histiocytes w...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/82.6.734

    authors: Szporn AH,Dikman S,Jagirdar J

    更新日期:1984-12-01 00:00:00

  • Use of viral cultures and serologic tests for cytomegalovirus infection. Rational or random?

    abstract::To assess their applied clinical utility, viral cultures and serological tests for cytomegalovirus (CMV) were reviewed at Duke University Medical Center (DUMC), a 1,125-bed tertiary-care hospital. Less than 1% (3 of 1,216) of CMV cultures were positive, and 8% of serum samples (45 of 587) were positive by single sera ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.2.180

    authors: Mangano WE,Gruninger RP

    更新日期:1996-08-01 00:00:00

  • Prevalence of non-A,non-B hepatitis/hepatitis C virus antibody in human immunoglobulins.

    abstract::Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/97.1.108

    authors: Dodd LG,McBride JH,Gitnick GL,Howanitz PJ,Rodgerson DO

    更新日期:1992-01-01 00:00:00

  • Statin--a novel marker of nonproliferation. Expression in nonneoplastic lymphoid tissues and follicular lymphomas.

    abstract::Statin is a 57 kDa nuclear protein exclusively found in noncycling cells. Its expression in hematolymphoid cells had not been examined previously. The authors studied statin immunoreactivity in nonneoplastic lymphoid tissues from 26 lymph nodes, 3 tonsils, and 2 spleens. Statin was detected primarily in small lymphoid...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.4.421

    authors: Trudel MA,Oligny L,Caplan S,Caplan C,Schipper HM,Wang E

    更新日期:1994-04-01 00:00:00

  • Penicillin susceptibility of Streptococcus pneumoniae in 1978. Screening for resistance by disk testing.

    abstract::Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.2.177

    authors: Maki DG,Helstad AG,Kimball JL

    更新日期:1980-02-01 00:00:00

  • Diagnostic utility of carboxyl-terminal and intact parathyroid hormone immunoassays in hospitalized patients.

    abstract::In a university-affiliated community hospital, medical records of 58 patients on whom the intact parathyroid hormone immunoassay (I-PTH) and 29 patients on whom both the carboxyl terminal PTH(C-PTH) and I-PTH ordered by physicians were reviewed to determine the reasons for requesting these tests. Reasons for ordering ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/77.6.720

    authors: Juan D,Wehrmeister J

    更新日期:1982-06-01 00:00:00

  • Detection of chlamydial cervicitis by Papanicolaou stained smears and culture.

    abstract::The ability of the cervical Papanicolaou (Pap) smear to detect cervicitis associated with Chlamydia trachomatis was investigated. Cultures and cervical cytology samples were obtained from 487 women seen at the Mayo Clinic Department of Obstetrics and Gynecology and at the Sexually Transmitted Disease clinic of the Olm...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.4.421

    authors: Dorman SA,Danos LM,Wilson DJ,Noller KL,Malkasian GD,Goellner JR,Smith TF

    更新日期:1983-04-01 00:00:00

  • Measuring direct thrombin inhibitors with routine and dedicated coagulation assays: which assay is helpful?

    abstract::The use of direct thrombin inhibitors (DTIs) for prophylactic or therapeutic anticoagulation is increasing because of the predictable bioavailability and short half-life of these DTIs. However, in certain situations, indication of the concentration is warranted. We investigated the effects of 3 DTIs (lepirudin, argatr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPQOD9WFPEYY0H

    authors: Curvers J,van de Kerkhof D,Stroobants AK,van den Dool EJ,Scharnhorst V

    更新日期:2012-10-01 00:00:00

  • Lymphomatoid papulosis. Characterization of skin infiltrates by monoclonal antibodies.

    abstract::Cryostat sections from fully developed papular lesions of lymphomatoid papulosis (histologic subtype A or B) have been examined by immunoenzymatic staining with 24 monoclonal antibodies against lymphoid cells and their subsets. The lesions demonstrated essentially identical cellular compositions and consisted of T-lym...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.5.587

    authors: Ralfkiaer E,Stein H,Wantzin GL,Thomsen K,Ralfkiaer N,Mason DY

    更新日期:1985-11-01 00:00:00

  • High-density lipoprotein cholesterol. Significant bias between methods.

    abstract::Cholesterol content of the high-density lipoproteins has become of interest in evaluating the risk of development of coronary heart disease. There is an inverse relationship between high-density lipoprotein cholesterol and risk. The authors found a significant bias (11.1 mg/dl; 0.289 mmol/l) in a comparison of a rapid...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/71.5.564

    authors: Gerson B,Dean L,Copeland BE

    更新日期:1979-05-01 00:00:00

  • Direct comparison of two mechanized systems for identification of gram-negative bacilli. Autobac ID system versus the auto microbic system (with EBC plus).

    abstract::The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.4.462

    authors: Barry AL,Gavan TL,Badal RE,Telenson MJ

    更新日期:1982-10-01 00:00:00

  • Secondary massive ovarian edema with Meig's syndrome.

    abstract::Primary massive ovarian edema is uncommon, particularly when it involves both ovaries before puberty. Ovarian edema secondary to a preexisting ovarian lesion is one of the postulated mechanisms. A prepubertal girl presented with bilateral massive ovarian edema with stromal hyperthecosis, which may have predisposed bot...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.5.597

    authors: Lacson AG,Alrabeeah A,Gillis DA,Salisbury S,Grantmyre EB

    更新日期:1989-05-01 00:00:00

  • Serum enzyme alterations in polymyositis. Possible pitfalls in diagnosis.

    abstract::We report serum enzyme patterns in three patients with polymyositis or dermatomyositis whose cases posed potential problems in distinguishing malignancy, hepatocellular damage, and myocardial infarction from myositis. The alanine aminotransferase showed five- to 16-fold elevations. The creatine kinase MB isoenzyme and...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.4.556

    authors: Morton BD 3rd,Statland BE

    更新日期:1980-04-01 00:00:00

  • Immunohistochemical analysis of oral lichen-planus-like eruption in graft-versus-host disease after allogeneic bone marrow transplantation.

    abstract::Immunohistochemical analysis of oral lichen-planus-like eruption (LPLE) in graft-versus-host disease (GVHD) using monoclonal antibodies (MoAbs) was performed on five patients after allogeneic bone marrow transplantation (BMT) for leukemia. In the mucosal lesions of LPLE in GVHD, the major population of infiltrated lym...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.2.177

    authors: Fujii H,Ohashi M,Nagura H

    更新日期:1988-02-01 00:00:00

  • Toward Improving Practices for Submission of Diagnostic Tissue Blocks for National Cancer Institute Clinical Trials.

    abstract:OBJECTIVES:The National Cancer Institute (NCI) National Clinical Trials Network performs phase II and III clinical trials, which increasingly rely on the submission of diagnostic formalin-fixed, paraffin-embedded tissue blocks for biomarker assessment. Simultaneously, advances in precision oncology require that clinica...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqz141

    authors: Makhlouf H,Watson MA,Lankes HA,Weil C,Dickler M,Birrer M,Moskaluk C,Ramirez NC,Okby N,Alonsozana E,Barnes M,Goldman EB,Enos R,Lubensky I

    更新日期:2020-01-02 00:00:00

  • Comparison of a standard and a sensitive thromboplastin in monitoring low intensity oral anticoagulant therapy.

    abstract::The greater precision in prothrombin time monitoring obtained using thromboplastins with low international sensitivity index (ISI) values are believed to result in improved patient care. The authors conducted a blinded prospective study of 84 random patients on low-intensity warfarin therapy who were monitored with ei...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1093/ajcp/102.1.134

    authors: Brophy MT,Fiore LD,Lau J,Goodwin R,Lopez A,Deykin D

    更新日期:1994-07-01 00:00:00

  • Performance characteristics of nested polymerase chain reaction vs real-time polymerase chain reaction methods for detecting Mycobacterium tuberculosis complex in paraffin-embedded human tissues.

    abstract:OBJECTIVES:Nucleic acid amplification tests on formalin-fixed, paraffin-embedded (FFPE) tissue specimens enable Mycobacterium tuberculosis complex (MTB) detection and rapid tuberculosis diagnosis in the absence of microbiologic culture tests. We aimed to evaluate the efficacy of different polymerase chain reaction (PCR...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP2QZRH4ZNPRDD

    authors: Seo AN,Park HJ,Lee HS,Park JO,Chang HE,Nam KH,Choe G,Park KU

    更新日期:2014-09-01 00:00:00

  • Pre-instrumental variables in coagulation testing.

    abstract::A number of variables thought to affect measurement of prothrombin time (PT) and partial thromboplastin time (PTT) were examined in an effort to determine more precisely their effects on these measurements. On the basis of these studies, it is proposed that blood specimens be anticoagulated with one part 3.8% (w./v.) ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.5.591

    authors: Koepke JA,Rodgers JL,Ollivier MJ

    更新日期:1975-11-01 00:00:00

  • Comparison of anti-estrogen receptor antibodies SP1, 6F11, and 1D5 in breast cancer: lower 1D5 sensitivity but questionable clinical implications.

    abstract::We compared the anti-estrogen receptors (ER) SP1, 6F11, and 1D5 antibodies in breast carcinoma cases with different ranges of positive cells to evaluate whether this could generate different therapies for patients. We selected 66 cases of breast cancer, each of which was immunostained with the 3 antibodies. 1D5 was le...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPLX0QJROV2IJG

    authors: Bogina G,Zamboni G,Sapino A,Bortesi L,Marconi M,Lunardi G,Coati F,Massocco A,Molinaro L,Pegoraro C,Venturini M

    更新日期:2012-11-01 00:00:00

  • The Prognosis of Stage IA Mixed Endometrial Carcinoma.

    abstract:OBJECTIVES:To explore the survival and definition of stage IA mixed endometrial carcinoma. METHODS:From June 1, 2010, to June 1, 2017, cases with stage IA endometrial cancer were included in this study. The survival outcomes were compared among patients with endometrioid (group A), nonendometrioid (group B), and mixed...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqz083

    authors: Li W,Li L,Wu M,Lang J,Bi Y

    更新日期:2019-10-07 00:00:00

  • Squamous metaplasia of the prostate. An immunohistochemical study.

    abstract::Immunoperoxidase strains for prostate-specific antigen (PSA), prostatic acid phosphatase (PAcP), epithelial membrane antigen (EMA), and cytokeratins (MAK 6 and CK-KES) were performed on 1 case of squamous cell carcinoma of the prostate and on 13 cases of squamous metaplasia of prostatic epithelium in an effort to demo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/90.5.597

    authors: Lager DJ,Goeken JA,Kemp JD,Robinson RA

    更新日期:1988-11-01 00:00:00

  • Rapid diagnosis of actinomycosis by thin-needle aspiration biopsy.

    abstract::Actinomycosis was diagnosed in three cases by the use of thin-needle aspiration biopsy technic. Aspiration was utilized for morphologic studies and collection of material for microbiologic isolation. The critical histologic features of sulfur granules remain intact with aspiration technic. Thin-needle aspiration biops...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.1.27

    authors: Pollock PG,Meyers DS,Frable WJ,Valicenti JF Jr,Koontz FP,Beavert CS

    更新日期:1978-07-01 00:00:00

  • Small cell carcinoma of the endometrium in a 23-year-old woman: light microscopic and ultrastructural study.

    abstract::The case of a 23-year-old woman with a primary small cell carcinoma of the endometrium is reported. The light microscopic features were similar to those of small cell carcinoma of the lung and those of the uterine cervix. Electron microscopy revealed cytoplasmic neurosecretory type granules and indistinct cell junctio...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.1.98

    authors: Kumar NB

    更新日期:1984-01-01 00:00:00

  • Solar elastosis in cutaneous melanoma.

    abstract::By studying more than 1,200 patients with cutaneous melanoma and long-term follow-up, I examined the relationship between solar elastosis and age at diagnosis of melanoma, key features of melanoma, and the outcome of overall survival in melanoma. I found that melanomas with elastosis were diagnosed significantly later...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/7MHX96XH3DTY32TQ

    authors: Vollmer RT

    更新日期:2007-08-01 00:00:00

  • Marrow biopsy and survival in multiple myeloma.

    abstract::Pretreatment posterior iliac crest bone marrow biopsies were obtained from 26 multiple myeloma patients. Biopsies were processed in methacrylate. The percentage of myeloma tissue, myeloid tissue, and fat was estimated in each biopsy. In 20 patients who died there was a positive correlation between the percent of myelo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.3.363

    authors: Mahmoud LA,Block MH,Franks JJ,Sayed NM

    更新日期:1983-09-01 00:00:00

  • Oxalosis. An unusual cause of myelophthisis in childhood.

    abstract::In a child with renal failure and oliguria due to hyperoxaluria myelophthisis developed as a result of extensive bone-marrow replacement with calcium oxalate crystals and an accompanying fibrous proliferations. The histopathology associated with this metabolic disorder was demonstrated in posterior iliac crest bone-ma...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/66.6.991

    authors: McKenna RW,Dehner LP

    更新日期:1976-12-01 00:00:00