Abstract：
OBJECTIVES:Nucleic acid amplification tests on formalin-fixed, paraffin-embedded (FFPE) tissue specimens enable Mycobacterium tuberculosis complex (MTB) detection and rapid tuberculosis diagnosis in the absence of microbiologic culture tests. We aimed to evaluate the efficacy of different polymerase chain reaction (PCR) methods for detecting Mycobacterium species in FFPE tissues. METHODS:We examined 110 FFPE specimens (56 nonmycobacterial cases, 32 MTB, and 22 nontuberculous mycobacteria [NTM] determined by acid-fast bacilli [AFB] culture) to assess five PCR methods: nested PCR (N-PCR) (Seeplex MTB Nested ACE Detection; Seegene, Seoul, South Korea), an in-house real-time PCR (RT-PCR) method, and three commercial RT-PCR methods (AccuPower MTB RT-PCR [Bioneer, Seoul, Korea], artus M tuberculosis TM PCR [Qiagen, Hilden, Germany], and AdvanSure tuberculosis/NTM RT-PCR [LG Life Sciences, Seoul, Korea]). RESULTS:The results of N-PCR, in-house RT-PCR, and AdvanSure RT-PCR correlated well with AFB culture results (concordance rates, 94.3%, 87.5%, and 89.5%, respectively). The sensitivity of N-PCR (87.5%) was higher than that of the RT-PCR methods, although these differences were not statistically significant between N-PCR and the in-house and AdvanSure RT-PCR methods (68.8% and 80.0%, respectively). All the PCR methods had high specificities, ranging from 98.2% to 100%. Only two NTM cases were detected by AdvanSure RT-PCR, implying a very low sensitivity. CONCLUSIONS:Well-designed RT-PCR and N-PCR can effectively identify MTB in FFPE specimens.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Seo AN,Park HJ,Lee HS,Park JO,Chang HE,Nam KH,Choe G,Park KUdoi
10.1309/AJCP2QZRH4ZNPRDDsubject
Has Abstractpub_date
2014-09-01 00:00:00pages
384-90issue
3eissn
0002-9173issn
1943-7722pii
142/3/384journal_volume
142pub_type
杂志文章abstract：:The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.4.462
更新日期：1982-10-01 00:00:00
abstract：:A patient receiving antihypertensive therapy developed acute intravascular hemolysis and died. Hemolysis was due to an immune process associated with antibody to thiazide. Only two other cases have been reported. Thiazide-induced hemolysis appears to be confined to those patients treated concommitantly with methyldopa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.6.791
更新日期：1984-06-01 00:00:00
abstract：OBJECTIVES:Fundic gland polyps (FGPs) occur in two clinical settings, sporadic and syndromic. Epithelial dysplasia is rare in sporadic FGPs, and progression data from large series are lacking. The aim of this study was to evaluate the clinical, morphologic, and immunohistochemical features in a large series of sporadic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPGK8QTYPUQJYL
更新日期：2015-10-01 00:00:00
abstract：:Interest in the role of mononuclear phagocytes in glomerulonephritis (GN) and in defining markers of renal neoplasms led the authors to study alpha-naphthyl acetate/butyrate esterase (ANAE/ANBE), alkaline phosphatase (AlkP), acid phosphatase (AcP), 5'-nucleotidase (5'N), and ATPase (ATP) activity in paraformaldehyde-f...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/83.5.605
更新日期：1985-05-01 00:00:00
abstract：:This study evaluates the sensitivity and detection time of a new radiometric broth (BACTEC 13A medium, Johnston Laboratories, Towson, MD) designed for direct blood culturing for Mycobacteria. A total of 1,848 blood specimens were cultured in parallel by a new method in which blood was directly inoculated into 13A medi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.3.316
更新日期：1989-03-01 00:00:00
abstract：:Immunoperoxidase strains for prostate-specific antigen (PSA), prostatic acid phosphatase (PAcP), epithelial membrane antigen (EMA), and cytokeratins (MAK 6 and CK-KES) were performed on 1 case of squamous cell carcinoma of the prostate and on 13 cases of squamous metaplasia of prostatic epithelium in an effort to demo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.5.597
更新日期：1988-11-01 00:00:00
abstract：:The measurement of beta-thromboglobulin (BTG) and platelet lactic dehydrogenase (LDH) as markers for alpha-granule release and platelet lysis, respectively, has been reported to correlate with the amount of damage incurred during the preparation and storage of platelet concentrates. We compared the concentrations of t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.6.719
更新日期：1983-06-01 00:00:00
abstract：:Although prednisolone is the most common immunosuppressive agent used in renal transplantation, an accurate formulation of the optimal dose regimen remains to be established. A sensitive and precise radioimmunoassay was developed for this purpose. Fifty microliters of 1:20 dilutions of serum or 1:200 dilutions of urin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.6.804
更新日期：1980-06-01 00:00:00
abstract：:Evaluation of blood cells to determine immunologic status is becoming an important clinical application of flow cytometric analysis. For a wider use of immunophenotyping technology in clinical laboratories, the authors developed a rapid method to detect monoclonal antibody-labeled cells using forward light scatter/abs...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/97.3.331
更新日期：1992-03-01 00:00:00
abstract：:Three policies for decreasing unnecessary cerebrospinal fluid (CSF) management Venereal Disease Research Laboratory (VDRL) tests were compared. The first policy attempted to educate physicians about the use of serologic tests for diagnosing neurosyphilis but allowed the CSF VDRL to be performed either as a screening t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.3.397
更新日期：1991-03-01 00:00:00
abstract：OBJECTIVES:B-lymphoblastic leukemia (B-LBL) arising in patients with chronic lymphocytic leukemia (CLL) is exceedingly rare and poorly characterized. METHODS:We describe four patients with CLL and concurrent or subsequent B-LBL diagnosed by morphologic, immunophenotypic, cytogenetic, and molecular analysis and reviewe...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPXE5VMONMVLZ0
更新日期：2015-08-01 00:00:00
abstract：:Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.1.35
更新日期：1991-01-01 00:00:00
abstract：:Although the relationship between pathologic features and clinical outcome is well established in adult adrenocortical neoplasms, the prognostic value of these features in pediatric adrenocortical neoplasms (PACN) is unclear. In a series of PACNs from 54 Brazilian children, the authors retrospectively investigated the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.5.625
更新日期：1994-05-01 00:00:00
abstract：:Remarkable improvements have been made in blood safety since the onset of the HIV epidemic. However, the current paradigm does not prevent all transfusion-transmitted infections and is reactive to new agents, thus accepting that some patients may be harmed before preventive measures are introduced. Several methods are...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/RAPQ3NXG3MV9AL94
更新日期：2007-12-01 00:00:00
abstract：:We performed a retrospective clinicopathologic study on sequential biopsy specimens from 90 patients with Philadelphia chromosome-positive chronic myelogenous leukemia to study therapy-specific effects of busulfan (28 patients), hydroxyurea (32 patients), and interferon-alfa (IFN-alfa; 30 patients). Bone marrow specim...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/XMGX-7HQ8-7PLU-LQ9M
更新日期：2000-07-01 00:00:00
abstract：:Establishment of a procedure termed cerebrospinal fluid (CSF) TRAP ("Transport and Rapid Accessioning for Additional Procedures") allows clinicians to appropriately store, at -75 degrees C, and rapidly access CSF specimens. The CSF TRAP enhances patient care by decreasing the need for repeat lumbar punctures and provi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.6.707
更新日期：1988-12-01 00:00:00
abstract：:Histologic follow-up of patients with the Bethesda system cervical-vaginal diagnosis of atypical glandular cells of undetermined significance (AGUS), "favor endocervical origin", or "not otherwise specified" (NOS) shows a high percentage of clinically significant (neoplastic or preneoplastic) lesions. Using the criter...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.3.299
更新日期：1997-03-01 00:00:00
abstract：:Transbronchoscopic biopsies of lung (transbronchial) or bronchus (endobronchial) have a high diagnostic of yield when performed by a single expert bronchoscopist or a small group of expert bronchoscopists. This procedure's diagnostic yield was evaluated in a general hospital where biopsies are performed by a diverse g...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.6.926
更新日期：1979-12-01 00:00:00
abstract：:This study investigates the dispensing and inoculating characteristics of the MIC-2000 micro-broth dilution system and compares its ability to determine minimum inhibitory concentrations with that of the reference macro-broth dilution method. Micro-well filling showed a coefficient of variation of 6.8%, and micro-well...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.3.374
更新日期：1980-03-01 00:00:00
abstract：:Invasion of the visceral pleura is an important component of lung carcinoma staging, and in some studies is an independent prognostic indicator. Evaluation of invasion by H&E may be indeterminate. Elastic stains can be helpful but are performed rarely. We reviewed all lung carcinoma resections from 1993 for 13 histolo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/112.6.777
更新日期：1999-12-01 00:00:00
abstract：:Sex cord tumor with annular tubules (SCTAT) is a distinctive ovarian sex cord-stromal tumor. The authors describe a case of SCTAT arising outside the ovaries, in the fallopian tube. The authors are aware of only one other case of extra-ovarian SCTAT, in an umbilical hernia sac in an adult woman. The case reported was ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/96.2.259
更新日期：1991-08-01 00:00:00
abstract：:Urine myoglobin concentrations are measured clinically to assess rhabdomyolysis and the related risk of renal damage. We studied urine myoglobin concentrations in vitro to explore the factors affecting stability. Myoglobin was very unstable in urine specimens, especially below pH 6.5, and its immunoreactivity deterior...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/9AQ6-2FR2-65ER-3E2W
更新日期：2005-03-01 00:00:00
abstract：Objectives:The aims were to evaluate breast cancer (BC) tumor-infiltrating lymphocytes (TILs) interobserver variability, heterogeneity, and the fidelity of scoring TILs in core needle biopsy (CNB). Methods:Matching CNB and two full-face sections (FFSs) of BC cases (n = 100) were independently reviewed by two pathologi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqy069
更新日期：2018-10-01 00:00:00
abstract：:Nineteen patients with acute nonlymphocytic leukemia were found to have a pericentric inversion of chromosome 16. All were classified as having acute myelomonocytic leukemia (M4) according to French-American-British Cooperative Group (FAB) criteria. Consistent abnormalities of marrow eosinophils were present. In 15 of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.6.733
更新日期：1984-06-01 00:00:00
abstract：:A markedly prolonged activated partial thromboplastin time (APTT) was observed in a 61-year-old woman with bruising and a decreasing hematocrit. Coagulation laboratory evaluation was sought to determine the cause of the prolonged APTT and bleeding. Evaluation demonstrated that, rather than identifying a coagulopathy, ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.2.108
更新日期：1993-08-01 00:00:00
abstract：:A murine monoclonal antibody (MAb 12C3) that is specific to human ovarian carcinomas was generated by immunizing mice with a human ovarian germinoma cell line (JOHYC-2). The antigen distribution that was defined by MAb 12C3 in normal and malignant human tissues was analyzed by immunohistochemistry on paraffin-embedded...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.3.288
更新日期：1995-03-01 00:00:00
abstract：:B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, includi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/29DA-CY9K-BHNW-4BG6
更新日期：2005-08-01 00:00:00
abstract：:The frequency with which blood gas measurements should be obtained is controversial because laboratory-based blood gas analyzers impose blood loss, delay, and cost on each measurement. Point-of-care analyzers eliminate the delay factor, but blood loss and cost factors remain. Optode microsensing can be adopted readily...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1995-10-01 00:00:00
abstract：:Statin is a 57 kDa nuclear protein exclusively found in noncycling cells. Its expression in hematolymphoid cells had not been examined previously. The authors studied statin immunoreactivity in nonneoplastic lymphoid tissues from 26 lymph nodes, 3 tonsils, and 2 spleens. Statin was detected primarily in small lymphoid...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.4.421
更新日期：1994-04-01 00:00:00
abstract：:The most common differentiated renal tumor of early childhood is the congenital mesoblastic neophroma. Well-differentiated forms of Wilms' tumor may present a difficult differential diagnosis from these benign neoplasms. The cases of two patients found to have well-differentiated renal neoplasms after being treated fo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/66.2.376
更新日期：1976-08-01 00:00:00
