Abstract:
:This study investigates the dispensing and inoculating characteristics of the MIC-2000 micro-broth dilution system and compares its ability to determine minimum inhibitory concentrations with that of the reference macro-broth dilution method. Micro-well filling showed a coefficient of variation of 6.8%, and micro-well inoculating showed a coefficient of variation of 6.1%. The reference macro-broth dilution method showed a coefficient of variation of 2.9% for dispensing and a coefficient of variation of 2.9% for inoculating. Ninety-three and eight tenths per cent of macro-broth minimum inhibitory concentrations duplicated the macro-broth modal value, with 100% falling within +/-1 double-dilution step. Eighty-five per cent of micro-broth minimum inhibitory concentrations duplicated the micro-broth modal value, with 99.8% falling within +/-1 double-dilution step. When the micro-broth results were evaluated using the macro-broth modal minimum inhibitory concentration value as a reference point, 55.8% duplicated the macro-broth modal value, with 4.7% falling above it and the remainder being skewed below it, with 6.2% at -2 double-dilution steps. These findings indicate that minimum inhibitory concentrations measured by the MIC-2000 system are somewhat lower than those of the reference macro-broth method. As a result, the micro-broth minimum inhibitory concentrations show a wider dispersion when referenced to the macro-broth modal minimum inhibitory concentration than when referenced to their own modal value.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Woolfrey BF,Ramadei WA,Quall COdoi
10.1093/ajcp/73.3.374subject
Has Abstractpub_date
1980-03-01 00:00:00pages
374-9issue
3eissn
0002-9173issn
1943-7722journal_volume
73pub_type
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journal_title:American journal of clinical pathology
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更新日期:2007-05-01 00:00:00
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更新日期:1993-09-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1978-09-01 00:00:00
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更新日期:1982-10-01 00:00:00
abstract::The performance characteristics of the XE-2100 (Sysmex, Kobe, Japan) automated immature granulocyte (IG) count were studied. The automated IG count was compared with the manual morphology count and with a proposed reference flow cytometric count. The comparison data were analyzed by both least-squares and Passing-Babl...
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更新日期:2007-09-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2002-03-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2006-06-01 00:00:00
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更新日期:1986-05-01 00:00:00
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更新日期:2012-11-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1980-12-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2007-05-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.6.749
更新日期:1996-12-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2001-10-01 00:00:00
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更新日期:2011-01-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1983-10-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1976-07-01 00:00:00
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更新日期:2010-12-01 00:00:00
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更新日期:2004-09-01 00:00:00
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更新日期:1994-04-01 00:00:00
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更新日期:1977-12-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2003-07-01 00:00:00
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更新日期:1988-03-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
abstract::Soft-agar culture of transitional cell carcinoma colonies from urine, bladder irrigation fluid, and transurethral resection solid tumor specimens demonstrate generally better growth of tumor cell colonies from urine than from irrigation fluid. Growth of tumor colonies from urine was adequate to evaluate presence of tu...
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pub_type: 杂志文章
doi:10.1093/ajcp/78.5.690
更新日期:1982-11-01 00:00:00
abstract::To study the usefulness of calretinin as an immunohistochemistry marker in the diagnosis of cardiac myxoma (CM) and the origin of myxoma cells, we examined 24 CMs and 9 fetal hearts with immunohistochemical methods on formalin-fixed paraffin-embedded tissues. We compared 24 CMs with 10 mural thrombi, 6 jaw myxomas, an...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
abstract::An American black family in whom hemoglobin J Baltimore and beta (+)-thalassemia genes coexisted is described. The proposita is a 23-year-old woman with a hemoglobin (Hb) level of 11.5 g/dl, microcytic, hypochromic indices, increased values of Hbs A2 and F, and alpha/non-alpha synthetic ratio of 1.52. Hbs A and J Balt...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.6.843
更新日期:1981-06-01 00:00:00
abstract:OBJECTIVES:A systematic review of the literature was performed to identify whether minimum formalin fixation time may be reduced for reliable immunohistochemical assessment of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). METHODS:PubMed, EMBASE, and the Cochra...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCP96YDQSTYBXWU
更新日期:2014-07-01 00:00:00