The effects of under 6 hours of formalin fixation on hormone receptor and HER2 expression in invasive breast cancer: a systematic review.

Abstract:

OBJECTIVES:A systematic review of the literature was performed to identify whether minimum formalin fixation time may be reduced for reliable immunohistochemical assessment of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). METHODS:PubMed, EMBASE, and the Cochrane Library were systematically searched for studies addressing effects of brief tissue fixation (<6 hours) on the analysis of ER, PR, or HER2 expression in patients with breast cancer. RESULTS:Five publications reported effects of brief fixation on ER, PR, or HER2 expression. Four studies showed similar receptor expression of short fixation compared with recommended fixation time (6-72 hours). One publication found that a minimum fixation time of 6 to 8 hours is necessary for reliable ER results. CONCLUSIONS:Available data on the effect of brief fixation on receptor status are limited. However, brief fixation of very highly expressing breast cancers does not seem to alter ER, PR, and HER2 status. Nevertheless, scoring inconsistencies have been observed. Further research is required in larger study populations with more low-expressing cases for future validation.

journal_name

Am J Clin Pathol

authors

Kalkman S,Barentsz MW,van Diest PJ

doi

10.1309/AJCP96YDQSTYBXWU

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

16-22

issue

1

eissn

0002-9173

issn

1943-7722

pii

142/1/16

journal_volume

142

pub_type

杂志文章,评审
  • Adamantinoma of the pretibial soft tissue. Clinicopathologic features, differential diagnosis, and possible relationship to intraosseous disease.

    abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.1.108

    authors: Mills SE,Rosai J

    更新日期:1985-01-01 00:00:00

  • Hepatosplenic gamma/delta T-cell lymphoma in bone marrow. A sinusoidal neoplasm with blastic cytologic features.

    abstract::We report 8 cases of hepatosplenic T-cell lymphoma (HSTCL) involving bone marrow and correlate histologic findings with disease progression. Immunophenotypic analysis demonstrated mature, aberrant gamma/delta T-cell immunophenotypes. Isochromosome 7q was identified in 4 cases; 1 case showed the t(7;14)(q34;q13). Seven...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/BM40-YM6J-9T3X-MH8H

    authors: Vega F,Medeiros LJ,Bueso-Ramos C,Jones D,Lai R,Luthra R,Abruzzo LV

    更新日期:2001-09-01 00:00:00

  • Babesiosis: problems in diagnosis using autoanalyzers.

    abstract::A 76-year-old white man previous diagnosed as having Waldenstrom's macroglobulinemia continued with persistent fevers and sweats for two and a half years. Recently, repeated automated differentials during 11 days of hospitalization failed to note any intracellular inclusions in the RBCs. Blood sent to the Microbiology...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.4.520

    authors: Bruckner DA,Garcia LS,Shimizu RY,Goldstein EJ,Murray PM,Lazar GS

    更新日期:1985-04-01 00:00:00

  • beta-Catenin is expressed aberrantly in tumors expressing shadow cells. Pilomatricoma, craniopharyngioma, and calcifying odontogenic cyst.

    abstract::We studied the beta-catenin immunohistochemical profile in tumors expressing shadow cells: pilomatricoma, 10 cases; calcifying odontogenic cyst, 6 cases; and craniopharyngioma, 9 cases. There was strong membranous, cytoplasmic, and nuclear staining of the immature basaloid cells in all of these tumors. Shadow cells we...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/EALE-G7LD-6W71-67PX

    authors: Hassanein AM,Glanz SM,Kessler HP,Eskin TA,Liu C

    更新日期:2003-11-01 00:00:00

  • Antithrombin and heparin antithrombin patterns in pre-thrombosis and thrombosis.

    abstract::An antithrombin assay (AA) and an antithrombin assay modified by the addition of heparin (H-AA) were performed using sera from healthy subjects, patients predisposed to thrombosis, and patients with thromboembolic disease. Characteristic AA, and H-AA patterns were found in each group. Normal controls and four of 39 "p...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/65.3.384

    authors: Innerfield I,Stone ML,Mersheimer W,Clauss RD,Greenberg J

    更新日期:1976-03-01 00:00:00

  • The defined antigen substrate sphere system with direct immunohistoperoxidase for detection of soluble dengue antigen in sera of patients with dengue hemorrhagic fever.

    abstract::The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.3.343

    authors: Boonpucknavig S,Bunnag C,Vuttiviroj O,Nimmannitya S

    更新日期:1978-09-01 00:00:00

  • Evaluation of the DCL Syphilis-G enzyme immunoassay test kit for the serologic diagnosis of syphilis.

    abstract::Sera have been submitted to the authors' laboratory for syphilis testing at a dramatically increased rate since the start of the human immunodeficiency virus type 1 epidemic. Thus they were interested in evaluating a new enzyme immunoassay, the DCL Syphilis-G test kit (DCL-G, Diagnostic Chemicals Ltd., Oxford, CT) for...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/99.3.282

    authors: Nayar R,Campos JM

    更新日期:1993-03-01 00:00:00

  • Preventing Genetic Testing Order Errors With a Laboratory Utilization Management Program.

    abstract:OBJECTIVES:To characterize error rates for genetic test orders between medical specialties and in different settings by examining detailed order information. METHODS:We performed a retrospective analysis of a detailed utilization management case database, comprising 2.5 years of data and almost 1,400 genetic test orde...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqw105

    authors: Mathias PC,Conta JH,Konnick EQ,Sternen DL,Stasi SM,Cole BL,Astion ML,Dickerson JA

    更新日期:2016-08-01 00:00:00

  • Xanthogranulomatous pseudotumor of the vagina: evidence of a local response to an unusual bacterium (mucoid Escherichia coli).

    abstract::Utilization of nonconventional bacterial strains and electron microscopy occasionally may aid in the recognition of unusual microorganisms which fail to be demonstrated by more conventional bacterial stains. We report an unusual case of recurrent vaginal polyps, initially thought by physical examination to represent a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.5.637

    authors: Strate SM,Taylor WE,Forney JP,Silva FG

    更新日期:1983-05-01 00:00:00

  • Paravaginal wolffian duct (mesonephros) adenocarcinoma: a light and electron microscopic study.

    abstract::This is a report of an adenocarcinoma of mesonephric origin studied by light microscopy, electron microscopy, and immunocytochemistry. Unlike previous reports, our lesion was located paravaginally and not in the leaves of the broad ligament or in the cervix. The light microscopic features are similar to those of previ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.4.539

    authors: Hinchey WW,Silva EG,Guarda LA,Ordonez NG,Wharton JT

    更新日期:1983-10-01 00:00:00

  • Use of a probe to repeat sequence of the Y chromosome for detection of host cells in peripheral blood of bone marrow transplant recipients.

    abstract::In situ hybridization for the Y chromosome (Y-ISH) was used to identify residual host cells in the peripheral blood of 51 recipients of sex-mismatched allogeneic marrow not depleted of T cells following conditioning with high-dose cyclophosphamide and total body irradiation (TBI). One patient who rejected the graft sh...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.2.201

    authors: Przepiorka D,Thomas ED,Durnam DM,Fisher L

    更新日期:1991-02-01 00:00:00

  • Diagnostic Testing Approaches for Activated Protein C Resistance and Factor V Leiden: A Comparison of Institutional and National Provider Practices.

    abstract:Objectives:To analyze the economic impact of testing for activated protein C resistance (APC-R) due to factor V Leiden (FVL) mutation with APC-R with reflexive FVL genotyping (algorithmic approach) or genotyping alone. Methods:OptumLabs Data Warehouse (OLDW) data were used to assess testing approaches. Insurance claim...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqx033

    authors: Perez Botero J,Majerus JA,Strege AK,Johnson RD,Chen D,Pruthi RK

    更新日期:2017-06-01 00:00:00

  • Determination of the oxygen dissociation curve and P50 of whole blood. An evaluation of the Hem-O-Scan and instrumentation of laboratory systems.

    abstract::The Travenol Hem-O-Scan and the Instrumentation Laboratory (IL) system for determination of the oxygen dissociation curve and P50 values have been evaluated. Using the Hem-O-Scan, an oxygen dissociation curve and P50 value may be obtained on 2 microliter of blood in 40 min, though the pH is not measured. In the IL sys...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.3.395

    authors: Nelson MG,Savage GA,Cooke PJ,Lappin TR

    更新日期:1981-03-01 00:00:00

  • Bacteremia and postmortem microbiology in burned children.

    abstract::During a three-year period, Staphylococcus aureus and Pseudomonas aeruginosa were the organisms most commonly isolated from blood cultures of burned children. Microorganisms were considered to contribute to the cause of death in 17 of 20 patients who died from various complications of thermal injuries. Pseudomonas aer...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/63.4.502

    authors: Smith RF,Linares HA,Jorgensen JH

    更新日期:1975-04-01 00:00:00

  • Evaluation of two commercial tests for human immunodeficiency virus antigens in culture supernatant fluid.

    abstract::Two commercial enzyme-linked immunosorbent assays (EIAs) for human immunodeficiency virus (HIV) antigens were evaluated for sensitivity and reproducibility by use of supernatant fluid from cell cultures of peripheral mononuclear cells of 18 patients with acquired immunodeficiency syndrome (AIDS) and 12 asymptomatic HI...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.6.788

    authors: Jackson JB,Sannerud K,Rhame FS,Balfour HH Jr

    更新日期:1988-06-01 00:00:00

  • Evolution of reactive lymphadenopathy into lymphoma over a nine-year period. A clinicopathologic study.

    abstract::Reported is a case of lymphoma which was preceded for nine years by an apparently reactive lymphadenopathy. Original slides of the multiple lymphoid tissue samples are reviewed, with emphasis on the gradually increasing numbers of mitoses, atypical histiocytes, and eosinophils, suggestive of malignant transformation. ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/68.5.606

    authors: Hyland CH,Murad TM,Dismukes WE

    更新日期:1977-11-01 00:00:00

  • Use of viral cultures and serologic tests for cytomegalovirus infection. Rational or random?

    abstract::To assess their applied clinical utility, viral cultures and serological tests for cytomegalovirus (CMV) were reviewed at Duke University Medical Center (DUMC), a 1,125-bed tertiary-care hospital. Less than 1% (3 of 1,216) of CMV cultures were positive, and 8% of serum samples (45 of 587) were positive by single sera ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.2.180

    authors: Mangano WE,Gruninger RP

    更新日期:1996-08-01 00:00:00

  • Practical use of computerized hospital information systems to improve blood transfusion.

    abstract::Data collection can be enhanced with the use of computers. Care must be taken, however, to ensure that the data collected have a purpose and meet either the quality assessment needs or business functions of the transfusion service. Required data elements are frequently in several data repositories and must be merged t...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Butch SH

    更新日期:1997-04-01 00:00:00

  • Clinical and economic performance criteria for intraarterial and extraarterial blood gas monitors, with comparison with in vitro testing.

    abstract::The frequency with which blood gas measurements should be obtained is controversial because laboratory-based blood gas analyzers impose blood loss, delay, and cost on each measurement. Point-of-care analyzers eliminate the delay factor, but blood loss and cost factors remain. Optode microsensing can be adopted readily...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Shapiro BA

    更新日期:1995-10-01 00:00:00

  • Effectiveness of multiple initiatives to reduce blood component wastage.

    abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP42WMHSSTPHXI

    authors: Collins RA,Wisniewski MK,Waters JH,Triulzi DJ,Yazer MH

    更新日期:2015-03-01 00:00:00

  • Does the size matter?: Prostate weight does not predict PSA recurrence after radical prostatectomy.

    abstract::Previous studies suggest that low prostate weight is a significant negative prognostic factor for prostate cancer. In the current study, the data for 431 men who underwent radical retropubic prostatectomy between 1990 and 1998 were analyzed for association between prostate weight and various clinical and pathologic pa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPPHGXDI94SGAC

    authors: Davidson DD,Koch MO,Lin H,Jones TD,Biermann K,Cheng L

    更新日期:2010-04-01 00:00:00

  • Incidental coenurosis: larval cestode presenting as an axillary mass.

    abstract::This article describes the occurrence of a coenurus, the encysted larval stage of a cestode of the genus Multiceps, within the axilla of a 40-year-old Nigerian woman. The patient had a history of intraductal carcinoma of the breast two years prior to the finding of an ipsilateral axillary mass. Pathologic evaluation o...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.5.735

    authors: Kurtycz DF,Alt B,Mack E

    更新日期:1983-11-01 00:00:00

  • Expression of the Leu-8 antigen by B-cell lymphomas.

    abstract::The Leu-8 antigen is found on the surface of many hematologic cells, including many T- and B-lymphocytes. With the use of a frozen-section immunoperoxidase technic, 152 B-cell non-Hodgkin's lymphomas were examined for Leu-8 expression. Of these lymphomas, 53% expressed Leu-8. Subclassification of the lymphomas with th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/88.4.486

    authors: Michie SA,Garcia CF,Strickler JG,Dailey MO,Rouse RV,Warnke RA

    更新日期:1987-10-01 00:00:00

  • Primary osteomyelitis due to an anaerobic microorganism.

    abstract::Primary osteomyelitis in a teen-aged boy that mimicked Ewing's tumor radiologically showed small Gram-negative rods on the original smear. The organism isolated was an obligately anaerobic bacterium, finally identified as Clostridium sphenoides. This finding underlines the need for microbiologic analysis of orthopedic...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.3.385

    authors: Isenberg HD,Lavine LS,Painter BG,Rubins WH,Berkman JI

    更新日期:1975-09-01 00:00:00

  • Serum prostate-specific antigen and prostate pathology in men having simple prostatectomy.

    abstract::Prostate-specific antigen (PSA) is a sensitive and specific serum marker for monitoring disease activity in men with prostatic carcinoma. Despite reports of elevation of levels of this analyte in men with benign prostatic hyperplasia, no information is available correlating the serum levels with the actual prostatic a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/92.6.760

    authors: Brawer MK,Rennels MA,Nagle RB,Schifman R,Gaines JA

    更新日期:1989-12-01 00:00:00

  • Prognostic features in patients with stage T1 breast carcinoma and a 0.5-cm or less lymph node metastasis. Significance of lymph node hilar tissue invasion.

    abstract::Trends in surgical practice suggest that pathologists will encounter increased numbers of patients with small invasive ductal adenocarcinomas; small, if any, metastatic deposits in axillary lymph nodes (ALNs); and possibly fewer ALN specimens to examine. New prognostic histologic features may be needed in this environ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/111.1.21

    authors: Goldstein NS,Mani A,Vicini F,Ingold J

    更新日期:1999-01-01 00:00:00

  • Lupus anticoagulant associated with specific inhibition of factor VII in a patient with AIDS.

    abstract::A 37-year-old intravenous drug abuser with acquired immune deficiency syndrome showed elevated activated partial thromboplastin time (APTT) and prothrombin time, normal thrombin time and fibrinogen, and borderline low platelet counts. The patient subsequently had a fracture of the left zygomatic arch, which did not pr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.4.491

    authors: Ndimbie OK,Raman BK,Saeed SM

    更新日期:1989-04-01 00:00:00

  • Malakoplakia of palatine tonsil.

    abstract::This paper's purpose is to document a case of unilateral tonsillar malakoplakia. Escherichia coli was repeatedly cultured from the lesion, and the isolate was studied by expert microbiologists who found no abnormalities in its biologic properties. The course of the lesion was followed by repeated punch biopsies before...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.3.390

    authors: Kalfayan B,Seager GM

    更新日期:1982-09-01 00:00:00

  • An improved method for evaluation of blood coagulation in heparinized blood.

    abstract::A simple method designed to allow coagulation studies in heparinized blood was evaluated. Citrated plasma containing heparin was incubated with an anion-exchange resin commercially available in tablet form (Heparsorb). After centrifugation of the mixture, coagulation studies were carried out on the supernatant plasma....

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.1.60

    authors: Cowan JF,Khan MB,Vargo J,Joist JH

    更新日期:1981-01-01 00:00:00

  • Expression of vascular endothelial growth factor (VEGF) and VEGF receptor Flk-1 in benign, premalignant, and malignant prostate tissue.

    abstract::Vascular endothelial growth factor (VEGF) is one of the most potent mitogenic, highly specific tumor angiogenic factors, which acts via binding to 2 specific tyrosine kinase receptors. There are few studies analyzing VEGF receptor expression in prostate cancer cells, and results are contradictory. In an immunohistoche...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/1LBM-6X32-JH6W-ENUD

    authors: Köllermann J,Helpap B

    更新日期:2001-07-01 00:00:00