Abstract:
OBJECTIVES:A systematic review of the literature was performed to identify whether minimum formalin fixation time may be reduced for reliable immunohistochemical assessment of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). METHODS:PubMed, EMBASE, and the Cochrane Library were systematically searched for studies addressing effects of brief tissue fixation (<6 hours) on the analysis of ER, PR, or HER2 expression in patients with breast cancer. RESULTS:Five publications reported effects of brief fixation on ER, PR, or HER2 expression. Four studies showed similar receptor expression of short fixation compared with recommended fixation time (6-72 hours). One publication found that a minimum fixation time of 6 to 8 hours is necessary for reliable ER results. CONCLUSIONS:Available data on the effect of brief fixation on receptor status are limited. However, brief fixation of very highly expressing breast cancers does not seem to alter ER, PR, and HER2 status. Nevertheless, scoring inconsistencies have been observed. Further research is required in larger study populations with more low-expressing cases for future validation.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Kalkman S,Barentsz MW,van Diest PJdoi
10.1309/AJCP96YDQSTYBXWUsubject
Has Abstractpub_date
2014-07-01 00:00:00pages
16-22issue
1eissn
0002-9173issn
1943-7722pii
142/1/16journal_volume
142pub_type
杂志文章,评审abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.1.108
更新日期:1985-01-01 00:00:00
abstract::We report 8 cases of hepatosplenic T-cell lymphoma (HSTCL) involving bone marrow and correlate histologic findings with disease progression. Immunophenotypic analysis demonstrated mature, aberrant gamma/delta T-cell immunophenotypes. Isochromosome 7q was identified in 4 cases; 1 case showed the t(7;14)(q34;q13). Seven...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/BM40-YM6J-9T3X-MH8H
更新日期:2001-09-01 00:00:00
abstract::A 76-year-old white man previous diagnosed as having Waldenstrom's macroglobulinemia continued with persistent fevers and sweats for two and a half years. Recently, repeated automated differentials during 11 days of hospitalization failed to note any intracellular inclusions in the RBCs. Blood sent to the Microbiology...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.4.520
更新日期:1985-04-01 00:00:00
abstract::We studied the beta-catenin immunohistochemical profile in tumors expressing shadow cells: pilomatricoma, 10 cases; calcifying odontogenic cyst, 6 cases; and craniopharyngioma, 9 cases. There was strong membranous, cytoplasmic, and nuclear staining of the immature basaloid cells in all of these tumors. Shadow cells we...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/EALE-G7LD-6W71-67PX
更新日期:2003-11-01 00:00:00
abstract::An antithrombin assay (AA) and an antithrombin assay modified by the addition of heparin (H-AA) were performed using sera from healthy subjects, patients predisposed to thrombosis, and patients with thromboembolic disease. Characteristic AA, and H-AA patterns were found in each group. Normal controls and four of 39 "p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.3.384
更新日期:1976-03-01 00:00:00
abstract::The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.3.343
更新日期:1978-09-01 00:00:00
abstract::Sera have been submitted to the authors' laboratory for syphilis testing at a dramatically increased rate since the start of the human immunodeficiency virus type 1 epidemic. Thus they were interested in evaluating a new enzyme immunoassay, the DCL Syphilis-G test kit (DCL-G, Diagnostic Chemicals Ltd., Oxford, CT) for...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/99.3.282
更新日期:1993-03-01 00:00:00
abstract:OBJECTIVES:To characterize error rates for genetic test orders between medical specialties and in different settings by examining detailed order information. METHODS:We performed a retrospective analysis of a detailed utilization management case database, comprising 2.5 years of data and almost 1,400 genetic test orde...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw105
更新日期:2016-08-01 00:00:00
abstract::Utilization of nonconventional bacterial strains and electron microscopy occasionally may aid in the recognition of unusual microorganisms which fail to be demonstrated by more conventional bacterial stains. We report an unusual case of recurrent vaginal polyps, initially thought by physical examination to represent a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.5.637
更新日期:1983-05-01 00:00:00
abstract::This is a report of an adenocarcinoma of mesonephric origin studied by light microscopy, electron microscopy, and immunocytochemistry. Unlike previous reports, our lesion was located paravaginally and not in the leaves of the broad ligament or in the cervix. The light microscopic features are similar to those of previ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.4.539
更新日期:1983-10-01 00:00:00
abstract::In situ hybridization for the Y chromosome (Y-ISH) was used to identify residual host cells in the peripheral blood of 51 recipients of sex-mismatched allogeneic marrow not depleted of T cells following conditioning with high-dose cyclophosphamide and total body irradiation (TBI). One patient who rejected the graft sh...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.2.201
更新日期:1991-02-01 00:00:00
abstract:Objectives:To analyze the economic impact of testing for activated protein C resistance (APC-R) due to factor V Leiden (FVL) mutation with APC-R with reflexive FVL genotyping (algorithmic approach) or genotyping alone. Methods:OptumLabs Data Warehouse (OLDW) data were used to assess testing approaches. Insurance claim...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqx033
更新日期:2017-06-01 00:00:00
abstract::The Travenol Hem-O-Scan and the Instrumentation Laboratory (IL) system for determination of the oxygen dissociation curve and P50 values have been evaluated. Using the Hem-O-Scan, an oxygen dissociation curve and P50 value may be obtained on 2 microliter of blood in 40 min, though the pH is not measured. In the IL sys...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.3.395
更新日期:1981-03-01 00:00:00
abstract::During a three-year period, Staphylococcus aureus and Pseudomonas aeruginosa were the organisms most commonly isolated from blood cultures of burned children. Microorganisms were considered to contribute to the cause of death in 17 of 20 patients who died from various complications of thermal injuries. Pseudomonas aer...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/63.4.502
更新日期:1975-04-01 00:00:00
abstract::Two commercial enzyme-linked immunosorbent assays (EIAs) for human immunodeficiency virus (HIV) antigens were evaluated for sensitivity and reproducibility by use of supernatant fluid from cell cultures of peripheral mononuclear cells of 18 patients with acquired immunodeficiency syndrome (AIDS) and 12 asymptomatic HI...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/89.6.788
更新日期:1988-06-01 00:00:00
abstract::Reported is a case of lymphoma which was preceded for nine years by an apparently reactive lymphadenopathy. Original slides of the multiple lymphoid tissue samples are reviewed, with emphasis on the gradually increasing numbers of mitoses, atypical histiocytes, and eosinophils, suggestive of malignant transformation. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/68.5.606
更新日期:1977-11-01 00:00:00
abstract::To assess their applied clinical utility, viral cultures and serological tests for cytomegalovirus (CMV) were reviewed at Duke University Medical Center (DUMC), a 1,125-bed tertiary-care hospital. Less than 1% (3 of 1,216) of CMV cultures were positive, and 8% of serum samples (45 of 587) were positive by single sera ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.2.180
更新日期:1996-08-01 00:00:00
abstract::Data collection can be enhanced with the use of computers. Care must be taken, however, to ensure that the data collected have a purpose and meet either the quality assessment needs or business functions of the transfusion service. Required data elements are frequently in several data repositories and must be merged t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1997-04-01 00:00:00
abstract::The frequency with which blood gas measurements should be obtained is controversial because laboratory-based blood gas analyzers impose blood loss, delay, and cost on each measurement. Point-of-care analyzers eliminate the delay factor, but blood loss and cost factors remain. Optode microsensing can be adopted readily...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1995-10-01 00:00:00
abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP42WMHSSTPHXI
更新日期:2015-03-01 00:00:00
abstract::Previous studies suggest that low prostate weight is a significant negative prognostic factor for prostate cancer. In the current study, the data for 431 men who underwent radical retropubic prostatectomy between 1990 and 1998 were analyzed for association between prostate weight and various clinical and pathologic pa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPHGXDI94SGAC
更新日期:2010-04-01 00:00:00
abstract::This article describes the occurrence of a coenurus, the encysted larval stage of a cestode of the genus Multiceps, within the axilla of a 40-year-old Nigerian woman. The patient had a history of intraductal carcinoma of the breast two years prior to the finding of an ipsilateral axillary mass. Pathologic evaluation o...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.5.735
更新日期:1983-11-01 00:00:00
abstract::The Leu-8 antigen is found on the surface of many hematologic cells, including many T- and B-lymphocytes. With the use of a frozen-section immunoperoxidase technic, 152 B-cell non-Hodgkin's lymphomas were examined for Leu-8 expression. Of these lymphomas, 53% expressed Leu-8. Subclassification of the lymphomas with th...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.4.486
更新日期:1987-10-01 00:00:00
abstract::Primary osteomyelitis in a teen-aged boy that mimicked Ewing's tumor radiologically showed small Gram-negative rods on the original smear. The organism isolated was an obligately anaerobic bacterium, finally identified as Clostridium sphenoides. This finding underlines the need for microbiologic analysis of orthopedic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.3.385
更新日期:1975-09-01 00:00:00
abstract::Prostate-specific antigen (PSA) is a sensitive and specific serum marker for monitoring disease activity in men with prostatic carcinoma. Despite reports of elevation of levels of this analyte in men with benign prostatic hyperplasia, no information is available correlating the serum levels with the actual prostatic a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/92.6.760
更新日期:1989-12-01 00:00:00
abstract::Trends in surgical practice suggest that pathologists will encounter increased numbers of patients with small invasive ductal adenocarcinomas; small, if any, metastatic deposits in axillary lymph nodes (ALNs); and possibly fewer ALN specimens to examine. New prognostic histologic features may be needed in this environ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/111.1.21
更新日期:1999-01-01 00:00:00
abstract::A 37-year-old intravenous drug abuser with acquired immune deficiency syndrome showed elevated activated partial thromboplastin time (APTT) and prothrombin time, normal thrombin time and fibrinogen, and borderline low platelet counts. The patient subsequently had a fracture of the left zygomatic arch, which did not pr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.4.491
更新日期:1989-04-01 00:00:00
abstract::This paper's purpose is to document a case of unilateral tonsillar malakoplakia. Escherichia coli was repeatedly cultured from the lesion, and the isolate was studied by expert microbiologists who found no abnormalities in its biologic properties. The course of the lesion was followed by repeated punch biopsies before...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/78.3.390
更新日期:1982-09-01 00:00:00
abstract::A simple method designed to allow coagulation studies in heparinized blood was evaluated. Citrated plasma containing heparin was incubated with an anion-exchange resin commercially available in tablet form (Heparsorb). After centrifugation of the mixture, coagulation studies were carried out on the supernatant plasma....
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.1.60
更新日期:1981-01-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is one of the most potent mitogenic, highly specific tumor angiogenic factors, which acts via binding to 2 specific tyrosine kinase receptors. There are few studies analyzing VEGF receptor expression in prostate cancer cells, and results are contradictory. In an immunohistoche...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/1LBM-6X32-JH6W-ENUD
更新日期:2001-07-01 00:00:00