Comparison of three methods for measuring factor VIII levels in plasma.

Abstract:

:We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage clot-based and chromogenic factor VIII assays (r2 = 0.934), and the lowest correlation was between the 1-stage clot-based and antigenic factor VIII assays (r2 = 0.821). The presence of heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors in the sample resulted in major interference in the 1-stage clot-based assay but not the chromogenic or antigenic factor VIII assays. Overall, the chromogenic factor VIII activity assay was the optimal method, showing good precision, the best overall correlation with other assays, and no interference from heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors.

journal_name

Am J Clin Pathol

authors

Chandler WL,Ferrell C,Lee J,Tun T,Kha H

doi

10.1309/C8T8-YNB4-G3W4-5PRF

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

34-9

issue

1

eissn

0002-9173

issn

1943-7722

journal_volume

120

pub_type

杂志文章
  • Duplicate prealbumin bands in urine. Association with hepatorenal failure.

    abstract::By electrophoresis, prealbumin is seen as a discrete band anodic to the albumin fraction. Cases of duplicate bands in the prealbumin region have been described previously in serum, cerebrospinal fluid, and urine, and have had an association with varied clinical entities. The authors describe a novel association of sev...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.4.475

    authors: Castañeda L,Ruiz P

    更新日期:1994-04-01 00:00:00

  • Detection of viral hepatitis type A.

    abstract::Several technics are currently being used to detect hepatitis type A antigen or its antibody. These include immunoelectronmicroscopy, immune adherence, and complement fixation. This paper describes another promising technic, a microtiter solid-phase immunoradiometric assay, in which hepatitis A antigen and antibody ar...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Hollinger FB,Bradley DW,Dreesman GR,Melnick JL

    更新日期:1976-05-01 00:00:00

  • Morphologic and Immunocytochemical Features of High-Grade Serous Carcinoma of Ovary in Ascitic Fluid Effusion and Fine-Needle Aspiration Cytology.

    abstract:OBJECTIVES:High-grade serous carcinoma (HGSC) is the most common ovarian malignancy. The role of cytopathology in obtaining tissue diagnosis before institution of neoadjuvant chemotherapy (NACT) was evaluated. METHODS:All histopathology-proven HGSC specimens between 2015 and 2018 with prior cytopathologic diagnosis by...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa028

    authors: Bansal A,Srinivasan R,Rohilla M,Sundaram A,Rai B,Rajwanshi A,Suri V,Saha SC,Gupta N,Gupta P,Dey P

    更新日期:2020-06-08 00:00:00

  • False positive indirect fluorescent antibody (IFA) tests for antibody to herpes simplex virus 1. Comparison of four commercially available methods.

    abstract::Four commercially available herpes simplex virus 1 (HSV-1) indirect fluorescent antibody (IFA) kits were compared with the use of sera selected because they were negative for HSV antibody by complement fixation (CF less than 1:8) and by ELISA (less than 1:100). However, 14 of 24 (58.3%) of these HSV-1 antibody-negativ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.6.765

    authors: Rand KH,Houck HJ,Dickinson M

    更新日期:1986-12-01 00:00:00

  • Solar elastosis in cutaneous melanoma.

    abstract::By studying more than 1,200 patients with cutaneous melanoma and long-term follow-up, I examined the relationship between solar elastosis and age at diagnosis of melanoma, key features of melanoma, and the outcome of overall survival in melanoma. I found that melanomas with elastosis were diagnosed significantly later...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/7MHX96XH3DTY32TQ

    authors: Vollmer RT

    更新日期:2007-08-01 00:00:00

  • Hematologic and iron-related measurements in rheumatoid arthritis.

    abstract::The hematologic status of 265 patients with rheumatoid arthritis was assessed. In the group as a whole, a mild depression in the hemoglobin concentration and mean cell volume (MCV) was associated with an increase in the red blood cell distribution width (RDW), erythrocyte sedimentation rate (ESR), and platelet count. ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/87.2.196

    authors: Baynes RD,Bothwell TH,Bezwoda WR,Gear AJ,Atkinson P

    更新日期:1987-02-01 00:00:00

  • Lupus anticoagulant associated with specific inhibition of factor VII in a patient with AIDS.

    abstract::A 37-year-old intravenous drug abuser with acquired immune deficiency syndrome showed elevated activated partial thromboplastin time (APTT) and prothrombin time, normal thrombin time and fibrinogen, and borderline low platelet counts. The patient subsequently had a fracture of the left zygomatic arch, which did not pr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.4.491

    authors: Ndimbie OK,Raman BK,Saeed SM

    更新日期:1989-04-01 00:00:00

  • A comparative marker study of large cell lymphoma, Hodgkin's disease, and true histiocytic lymphoma in paraffin-embedded tissue.

    abstract::A comparative study of large cell lymphoma (LCL) (ten B and ten T), Hodgkin's disease (15 cases), and true histiocytic lymphoma (two cases) was undertaken, using formalin-fixed paraffin-embedded tissue sections, a panel of eight antibodies, and one lectin to determine if any particular antibody or immunologic profile ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.5.591

    authors: Meis JM,Osborne BM,Butler JJ

    更新日期:1986-11-01 00:00:00

  • Pathologic findings from the National Surgical Adjuvant Breast Project (protocol no. 4). VI. Invasive papillary cancer.

    abstract::Although papillary forms of intraductal cancer are a common component of amny mammary cancers, invasive papillary cancer of the breast is relatively rare. Only 35 examples were encountered in the specimens obtained from 1603 women participating in a prospective randomized study of invasive mammary cancer. Contingency-...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1093/ajcp/73.3.313

    authors: Fisher ER,Palekar AS,Redmond C,Barton B,Fisher B

    更新日期:1980-03-01 00:00:00

  • Aberrations of chromosomes 9 and 17 in bilharzial bladder cancer as detected by fluorescence in situ hybridization.

    abstract::Fluorescence in situ hybridization (FISH), using repetitive alpha satellite probes to chromosomes 9 and 17, was performed on 27 paraffinembedded bladder specimens from 18 Egyptian patients affected with bilharzial carcinoma. Fluorescence in situ hybridization results in the carcinomas and benign mucosa of patients wit...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.2.234

    authors: Ghaleb AH,Pizzolo JG,Melamed MR

    更新日期:1996-08-01 00:00:00

  • Advantages of a new anticoagulant in routine hematology on the Coulter Counter S-Plus STKR analyzer.

    abstract::The authors report the advantages of a new anticoagulant-antiaggregant mixture that avoids the deleterious effects of ethylenediaminotetraacetic acid (EDTA) on mean platelet volume and also prevents EDTA-induced platelet clumping. It is suitable for routine cell counting and sizing with the Coulter Counter S-Plus STKR...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/93.6.760

    authors: Lippi U,Schinella M,Modena N,Nicoli M,Lippi G

    更新日期:1990-06-01 00:00:00

  • Effects of cigarette smoking on intramyocardial arteries and arterioles in man.

    abstract::Proliferative lesions, which include collagen, elastic fibers and abnormally oriented muscle fibers, were found to develop in intramyocardial arteries more rapidly with age in cigarette smokers than in nonsmokers. The study attempted to determine whether these lesions reduce perfusion pressures in the subendocardial z...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/68.4.493

    authors: Naeye RL,Truong LD

    更新日期:1977-10-01 00:00:00

  • Steroid hormone receptor and COX-2 expression in chordoma.

    abstract::Reports of sex steroid receptor expression in chordoma suggest that these tumors may be responsive to hormone manipulation therapy. Immunohistochemical stains for estrogen receptor (ER)-alpha, ER-beta, progesterone receptor (PR), androgen receptor (AR), and cyclooxygenase 2 (COX-2), were performed on a tissue microarr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/8T2NPHLK5X5WQ3E7

    authors: Fasig JH,Dupont WD,Olson SJ,Lafleur BJ,Cates JM

    更新日期:2007-09-01 00:00:00

  • A rapid paper-disc test for penicillinase.

    abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.557

    authors: Tu KK,Jorgensen JH,Stratton CW

    更新日期:1981-04-01 00:00:00

  • Sensitivity, specificity, and reproducibility of four measures of laboratory turnaround time.

    abstract::The authors studied the performance of four measures of laboratory turnaround time: the mean, median, 90th percentile, and proportion of tests reported within a predetermined cut-off interval (proportion of acceptable tests [PAT]). Measures were examined with the use of turnaround time data from 11,070 stat partial th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.4.452

    authors: Valenstein PN,Emancipator K

    更新日期:1989-04-01 00:00:00

  • Expression of transketolase-like 1 and activation of Akt in grade IV glioblastomas compared with grades II and III astrocytic gliomas.

    abstract::Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/6H9844AMMET82DBJ

    authors: Völker HU,Hagemann C,Coy J,Wittig R,Sommer S,Stojic J,Haubitz I,Vince GH,Kämmerer U,Monoranu CM

    更新日期:2008-07-01 00:00:00

  • Blast-like cells in the cerebrospinal fluid of young infants: further characterization of clinical setting, morphology and origin.

    abstract::Blast-like cells in the cerebrospinal fluid (CSF) of neonates have been identified and previously suggested to be of germinal matrix origin. Twelve additional CSF specimens with blast-like cells collected at the University of Texas Medical Branch, Galveston, between 1985-1992 were analyzed. The cytological features of...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/105.5.544

    authors: Jaffey PB,Varma SK,DeMay RM,McLucas EJ,Campbell GA

    更新日期:1996-05-01 00:00:00

  • Inherited giant platelet disorders. Classification and literature review.

    abstract::Inherited giant platelet disorders are extremely rare. The aim of this article is to review the clinical and laboratory features of this heterogeneous group and to arrive at a working classification. We conducted our literature search using the National Library of Medicine database. A total of 12 clinical entities wer...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/FC4H-LM5V-VCW8-DNJU

    authors: Mhawech P,Saleem A

    更新日期:2000-02-01 00:00:00

  • Critical values in pediatric surgical pathology: definition, implementation, and reporting in a children's hospital.

    abstract::Timely communication of significant or unexpected findings in surgical pathology can significantly improve patient care. Although surgical pathology critical values have been published, no systematic assessment in pediatric surgical pathology has been published. We surveyed pediatric pathologists and pediatric subspec...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/Y5K829U67GVYDWTU

    authors: Coffin CM,Spilker K,Lowichik A,Zhou H,Nielson K,Erickson L,Pysher TJ

    更新日期:2007-12-01 00:00:00

  • Serum prostate-specific antigen and prostate pathology in men having simple prostatectomy.

    abstract::Prostate-specific antigen (PSA) is a sensitive and specific serum marker for monitoring disease activity in men with prostatic carcinoma. Despite reports of elevation of levels of this analyte in men with benign prostatic hyperplasia, no information is available correlating the serum levels with the actual prostatic a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/92.6.760

    authors: Brawer MK,Rennels MA,Nagle RB,Schifman R,Gaines JA

    更新日期:1989-12-01 00:00:00

  • Intrauterine infarction of a pulmonary segment in a full-term newborn. A case report.

    abstract::Intrauterine pulmonary infarction is extremely rare. In a series of 775 stillborn and newborn infants, only a few cases of acute pulmonary infarction were reported. The authors present the findings from a full-term infant who died at 9 hours of age of a chronic pulmonary infarction. Several pathogenic mechanisms, incl...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/100.6.701

    authors: Mirkin LD,Wong RC

    更新日期:1993-12-01 00:00:00

  • An improved method for evaluation of blood coagulation in heparinized blood.

    abstract::A simple method designed to allow coagulation studies in heparinized blood was evaluated. Citrated plasma containing heparin was incubated with an anion-exchange resin commercially available in tablet form (Heparsorb). After centrifugation of the mixture, coagulation studies were carried out on the supernatant plasma....

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.1.60

    authors: Cowan JF,Khan MB,Vargo J,Joist JH

    更新日期:1981-01-01 00:00:00

  • Cost minimization analysis of telepathology.

    abstract::Telepathology is gaining acceptance as a mode of providing pathology services to remote sites, but its economic feasibility is unknown. A dynamic robotic telepathology service between the Veterans Affairs Medical Center, Iron Mountain, MI, and the Veterans Affairs Medical Center, Milwaukee, WI, provides diagnostic ser...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/112.4.470

    authors: Agha Z,Weinstein RS,Dunn BE

    更新日期:1999-10-01 00:00:00

  • Osteoblastic osteogenic sarcoma in a 35-month-old girl. Report of a case.

    abstract::A girl, aged 2 years, 11 months, who had pain and swelling of the proximal humerus, was found to have osteoblastic osteogenic sarcoma of the humerus. Among 937 consecutive patients with osteogenic sarcoma examined at the Mayo Clinic, she is the youngest child seen, as well as only the sixth child younger than 6 years...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/63.6.886

    authors: Siegal GP,Dahlin DC,Sim FH

    更新日期:1975-06-01 00:00:00

  • Clinical chemistry determinations for a biracial cohort of 1,605 normal and hyperlipidemic schoolchildren aged 6 to 17.

    abstract::Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.4.371

    authors: Laskarzewski P,Kelly KA,Mellies MJ,Morrison JA,Khoury P,Tillett S,Glueck CJ

    更新日期:1980-10-01 00:00:00

  • Molecular Genetic Landscape of Sclerosing Pneumocytomas.

    abstract:OBJECTIVES:Sclerosing pneumocytomas are rare pulmonary neoplasms that are typically benign. However, rare patients experience progressive disease, and therapy targeting specific genetic underpinnings could be an attractive therapeutic option. Recent studies have found recurrent AKT 1 mutations in sclerosing pneumocytom...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa136

    authors: Boland JM,Lee HE,Barr Fritcher EG,Voss JS,Jessen E,Davila JI,Kipp BR,Graham RP,Maleszewski JJ,Yi ES

    更新日期:2020-11-04 00:00:00

  • Cytologic/histologic correlation for quality control in cervicovaginal cytology. Experience with 1,582 paired cases.

    abstract::For quality-control purposes, federal regulations require cytology laboratories to compare Papanicolaou smear and cervical biopsy reports, if available, and determine the cause of any discrepancies. The authors reviewed 56,497 cervicovaginal smears, of which 1,582 (2.8%) had a subsequent cervical biopsy or endocervica...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/103.1.32

    authors: Joste NE,Crum CP,Cibas ES

    更新日期:1995-01-01 00:00:00

  • Histologic processing of microbiopsies from cervical smears is diagnostically useful in selected cases.

    abstract::We analyzed the value in cervical cytology of a recently developed technique by which it is possible to remove thick tissue specimens, called microbiopsies, from cervical smears and to process them for histologic examination. In 12 (48%) of 25 cervical smears in which microbiopsies were found, the histologic sections ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/108.2.191

    authors: Mravunac M,Verbeek DH,Sanders-Eras M,Besseling R,Wijnen-Dubbers C,Smedts F

    更新日期:1997-08-01 00:00:00

  • Spurious hemoglobin Barts caused by bilirubin: a common interference mimicking an uncommon hemoglobinopathy.

    abstract::High-performance liquid chromatography (HPLC) is replacing electrophoresis for identification of hemoglobin variants. Our objective was to identify unknown tall peaks with elution times and shapes of hemoglobin Barts found on hemoglobin chromatograms that could not be confirmed by alkaline and acid gel electrophoresis...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/73WJ-V2E0-RTVQ-H0BB

    authors: Howanitz PJ,Kozarski TB,Howanitz JH,Chauhan YS

    更新日期:2006-04-01 00:00:00

  • Application of adrenocorticotropin assays in a routine clinical laboratory.

    abstract::The radioimmunoassay of ACTH was used in a routine laboratory to localize the site of the lesion in 20 patients with Cushing's syndrome. Eight of the patients had no detectable circulating ACTH and had adrenal tumors removed, 12 had high levels and were diagnosed as having pituitary Cushing's syndrome. Very high level...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.5.618

    authors: Broughton A

    更新日期:1975-11-01 00:00:00