A rapid paper-disc test for penicillinase.

Abstract:

:A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow color within 1 to 30 min of incubation indicated penicillinase production. These discs were tested against Staphylococcus aureus, Haemophilus influenzae, and Neisseria gonorrhoeae, and the assay was found to be a simple, rapid, and accurate method for detection of penicillinase.

journal_name

Am J Clin Pathol

authors

Tu KK,Jorgensen JH,Stratton CW

doi

10.1093/ajcp/75.4.557

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

557-9

issue

4

eissn

0002-9173

issn

1943-7722

journal_volume

75

pub_type

杂志文章
  • Forty Postmortem Examinations in COVID-19 Patients.

    abstract:OBJECTIVES:Although diffuse alveolar damage, a subtype of acute lung injury (ALI), is the most common microscopic pattern in coronavirus disease 2019 (COVID-19), other pathologic patterns have been described. The aim of the study was to review autopsies from COVID-19 decedents to evaluate the spectrum of pathology and ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa156

    authors: De Michele S,Sun Y,Yilmaz MM,Katsyv I,Salvatore M,Dzierba AL,Marboe CC,Brodie D,Patel NM,Garcia CK,Saqi A

    更新日期:2020-11-04 00:00:00

  • An automated method for the analysis of T-cell receptor repertoires. Rapid RT-PCR fragment length analysis of the T-cell receptor beta chain complementarity-determining region 3.

    abstract::The examination of T-cell receptor (TCR) repertoires has an important role in the study of lymphoproliferative disorders and autoimmune diseases. Analysis of the complementarity-determining region 3 (CDR3) of the TCR beta chain is used to assess the clonality of T-cell populations. We developed a rapid fluorescence-ba...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/111.5.683

    authors: Lue C,Mitani Y,Crew MD,George JF,Fink LM,Schichman SA

    更新日期:1999-05-01 00:00:00

  • Clinical chemistry determinations for a biracial cohort of 1,605 normal and hyperlipidemic schoolchildren aged 6 to 17.

    abstract::Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.4.371

    authors: Laskarzewski P,Kelly KA,Mellies MJ,Morrison JA,Khoury P,Tillett S,Glueck CJ

    更新日期:1980-10-01 00:00:00

  • Congenital-infantile fibrosarcoma. A clinicopathologic study of 10 cases and molecular detection of the ETV6-NTRK3 fusion transcripts using paraffin-embedded tissues.

    abstract::Congenital-infantile fibrosarcoma (CIFS) is a relatively indolent sarcoma that should be distinguished from more aggressive spindle cell sarcomas of childhood. CIFSs have been found to have a novel recurrent reciprocal translocation t(12;15)(p13;q25) resulting in the gene fusion ETV6-NTRK3 (ETS variant gene 6; neurotr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/3H24-E7T7-V37G-AKKQ

    authors: Sheng WQ,Hisaoka M,Okamoto S,Tanaka A,Meis-Kindblom JM,Kindblom LG,Ishida T,Nojima T,Hashimoto H

    更新日期:2001-03-01 00:00:00

  • Endometrial stromal sarcomas: immunoprofile with emphasis on HMB45 reactivity.

    abstract:OBJECTIVES:We describe the morphologic and immunohistochemical features of 17 endometrial stromal neoplasms, 16 sarcomas, and one stromal nodule. METHODS:We reviewed 35 cases interpreted as endometrial stromal neoplasms, but 17 high-grade endometrial stromal sarcomas (ESS) and one case of mixed endometrial sarcoma and...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPS88CMJRXZBWA

    authors: Albores-Saavedra J,Dorantes-Heredia R,Chablé-Montero F,Chanona-Vilchis J,Pérez-Montiel D,Lino-Silva LS,González-Romo MA,Ramírez-Jaramillo JM,Henson DE

    更新日期:2014-06-01 00:00:00

  • Thymoma, atypical thymoma, and thymic carcinoma. A novel conceptual approach to the classification of thymic epithelial neoplasms.

    abstract::Primary thymic epithelial neoplasms have been the subject of much controversy in recent years owing to the difficulties posed by these tumors for precise histopathologic typing and prognostication. A number of classification schemes using different terminology have been proposed, none of which has satisfactorily manag...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/111.6.826

    authors: Suster S,Moran CA

    更新日期:1999-06-01 00:00:00

  • Quantitative urine culture in patients with urinary tract infection and bacteremia.

    abstract::A study was made of quantitative urine cultures in bacteremic patients with urinary tract infection as the only identifiable source. These patients represent the most serious form of urinary tract infection and should be recognized as significant by the criteria used for interpreting quantitative urine cultures. In 83...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/85.5.616

    authors: Roberts FJ

    更新日期:1986-05-01 00:00:00

  • Cytochemistry of acid hydrolases in chronic B- and T-cell leukemias.

    abstract::Four acid hydrolases, acid phosphatase (AP), alpha-naphthyl acetate esterase (ANAE), beta-glucuronidase, and N-acetyl-beta-glucosaminidase, were determined cytochemically in peripheral blood lymphocytes from 50 patients with B and T chronic lymphocytic and prolymphocytic leukemias (CLL, PLL) and related disorders. Str...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.4.437

    authors: Crockard A,Chalmers D,Matutes E,Catovsky D

    更新日期:1982-10-01 00:00:00

  • Evaluation of initial and deeper sections of esophageal biopsy specimens for detection of intestinal metaplasia.

    abstract::There are wide variations in the preparation of histologic sections from endoscopic esophageal biopsy specimens. We evaluated serial step sections from 261 esophageal or gastroesophageal junction biopsies at 4 levels to determine the first level at which goblet cell metaplasia (GCM) was detected. Deeper step sections ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/MGRX-LQG9-QVRV-VMCG

    authors: Chitkara YK,Eyre CL

    更新日期:2005-06-01 00:00:00

  • Duplicate prealbumin bands in urine. Association with hepatorenal failure.

    abstract::By electrophoresis, prealbumin is seen as a discrete band anodic to the albumin fraction. Cases of duplicate bands in the prealbumin region have been described previously in serum, cerebrospinal fluid, and urine, and have had an association with varied clinical entities. The authors describe a novel association of sev...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.4.475

    authors: Castañeda L,Ruiz P

    更新日期:1994-04-01 00:00:00

  • Platelet 5-hydroxytryptamine storage in the carcinoid syndrome.

    abstract::The uptake and storage of 5-hydroxytryptamine by human platelets from normal donors and patients with the carcinoid syndrome are reported. When platelets were incubated in the presence of 3H-5-hydroxytryptamine (50 microM), an initial phase of rapid uptake was observed until 5-hydroxytryptamine levels of 1--2 microgra...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.4.628

    authors: Lewis JC,Moertel CG

    更新日期:1978-10-01 00:00:00

  • Clinical and laboratory efficiency of rapid screening urine cultures by nephelometry.

    abstract::Urine cultures represent 40-50% of specimens processed by hospital clinical microbiology laboratories. An accurate rapid screening method to detect positive urine cultures could greatly improve laboratory efficiency. Three hundred eighty-two unselected urines were screened by nephelometry at 2, 4, and 5 hours of shaki...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/77.3.305

    authors: Heinz T,Pezzlo M,Thrupp L

    更新日期:1982-03-01 00:00:00

  • Recombinant factor VIIa in the treatment of bleeding.

    abstract::Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. It has become apparent that rFVIIa is useful in controlling bleeding in a variety of clinical situations. This review attempts to collate and summarize the nonhemophilia...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/D0G0-C96V-05CJ-5B4J

    authors: Midathada MV,Mehta P,Waner M,Fink LM

    更新日期:2004-01-01 00:00:00

  • Measuring HER-2 in breast cancer. Immunohistochemistry, FISH, or ELISA?

    abstract::Measuring HER-2 is important for selecting optimal therapy and predicting prognosis in breast cancer patients. Current methods for evaluating HER-2 include measuring protein overexpression by immunohistochemistry (IHC), measuring gene copy number by fluorescent in situ hybridization (FISH), and measuring shed antigen ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/D76M-QEGU-62BT-HKER

    authors: Yeh IT

    更新日期:2002-06-01 00:00:00

  • Simplified neutrophil hexose monophosphate shunt assay on whole blood.

    abstract::Neutrophil function was determined in resting and phagocytic cells by an assay of hexose monophosphate shunt activity performed on small volumes of whole blood. This new micromethod clearly differentiated between zymosan-stimulated and unstimulated hexose monophosphate shunt activity. Furthermore, the whole blood micr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.1.88

    authors: King JW,McCall CE

    更新日期:1980-07-01 00:00:00

  • Microscopic evaluation of sputum specimens submitted for Mycobacterium tuberculosis culture.

    abstract::Using microscopic screening criteria employed in the study of sputum in acute bacterial pneumonia, the authors evaluated 644 sputum samples from 347 patients submitted for mycobacterial bacterial culture. After microscopic examination, these specimens were processed using standard mycobacterial laboratory technics. Sa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.3.361

    authors: Isaac-Renton JL,Puselja BB,Allen EA,Grzybowski S,Black WA

    更新日期:1985-09-01 00:00:00

  • A column chromatographic method for the combined analysis of creatine kinase-MB (CPK-MB) and lactate dehydrogenase 1,2 (LDH 1,2) isoenzymes.

    abstract::A column chromatographic method for the simultaneous single-eluate isolation of CPK-MB and LDH 1,2 has been evaluated. The method is rapid in that the entire isolation is completed in less than an hour and subsequent analysis can be performed using presently available spectrophotometric methods and reagents without th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/66.2.425

    authors: Lederer WH,Gerstbrein HL,McClintock WC

    更新日期:1976-08-01 00:00:00

  • Cerebrospinal fluid (CSF) TRAP. A method to improve CSF laboratory efficiency.

    abstract::Establishment of a procedure termed cerebrospinal fluid (CSF) TRAP ("Transport and Rapid Accessioning for Additional Procedures") allows clinicians to appropriately store, at -75 degrees C, and rapidly access CSF specimens. The CSF TRAP enhances patient care by decreasing the need for repeat lumbar punctures and provi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/90.6.707

    authors: Albright RE Jr,Christenson RH,Habig RL,Mears TP,Schneider KA

    更新日期:1988-12-01 00:00:00

  • Immunohistochemical demonstration of acid phosphatase isoenzyme 5 (tartrate-resistant) in paraffin sections of hairy cell leukemia and other hematologic disorders.

    abstract::The demonstration of tartrate-resistant acid phosphatase (TRAP) activity has long been a cornerstone in the diagnosis of hairy cell leukemia (HCL). Recently a monoclonal antibody to this enzyme has been developed that can be used in an immunoperoxidase method on paraffin-embedded tissues. By using a peroxidase-labeled...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/108.3.308

    authors: Hoyer JD,Li CY,Yam LT,Hanson CA,Kurtin PJ

    更新日期:1997-09-01 00:00:00

  • Unilocular acinar cell cystadenoma of the pancreas an unusual acinar cell tumor.

    abstract::We report an unusual case of acinar cell cystadenoma of the pancreas in a 52-year-old man treated for pulmonary adenocarcinoma. The lesion, located in the body of the pancreas, was revealed incidentally by abdominal computed tomography during follow-up for a pulmonary neoplasm. A left pancreatectomy was performed. The...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/JQQ1-06NY-0F3J-PEU9

    authors: Chatelain D,Paye F,Mourra N,Scoazec JY,Baudrimont M,Parc R,Flejou JF

    更新日期:2002-08-01 00:00:00

  • Fluorescent cytochemical detection of estrogen and progesterone receptors in breast fine-needle aspirates.

    abstract::Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.1.35

    authors: Masood S

    更新日期:1991-01-01 00:00:00

  • Molecular Genetic Landscape of Sclerosing Pneumocytomas.

    abstract:OBJECTIVES:Sclerosing pneumocytomas are rare pulmonary neoplasms that are typically benign. However, rare patients experience progressive disease, and therapy targeting specific genetic underpinnings could be an attractive therapeutic option. Recent studies have found recurrent AKT 1 mutations in sclerosing pneumocytom...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa136

    authors: Boland JM,Lee HE,Barr Fritcher EG,Voss JS,Jessen E,Davila JI,Kipp BR,Graham RP,Maleszewski JJ,Yi ES

    更新日期:2020-11-04 00:00:00

  • The effects of nonclassic pediatric bacterial pathogens on the usefulness of the Directigen latex agglutination test.

    abstract::Haemophilus influenzae type b, Escherichia coli, Neisseria meningitis, Streptococcus agalactiae, and Streptococcus pneumoniae are classically the predominant meningeal pathogens of children. The Directigen latex agglutination test identifies these pathogens by detecting specific antigens in cerebrospinal fluid (CSF) a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.6.729

    authors: Hill RB,Adams S,Gunn BA,Eberly BJ

    更新日期:1994-06-01 00:00:00

  • Optimization of plasma fluorogenic thrombin-generation assays.

    abstract::We optimized fluorogenic thrombin-generation assays with regard to sample volume, calibration, analytic corrections, and activation reagents. Lower sample volumes (40 vs 80 microL) were associated with better recovery of thrombin activity, lower interference due to absorbance of light, and higher total thrombin genera...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP6AY4HTRAAJFQ

    authors: Chandler WL,Roshal M

    更新日期:2009-08-01 00:00:00

  • Expression of matrix metalloproteinase-2 related to lymph node metastasis of oral squamous cell carcinoma. A clinicopathologic study.

    abstract::To investigate the effect of matrix-degrading enzymes on the malignant potential of oral squamous cell carcinoma, the expression of matrix metalloproteinase-2 (MMP-2/72-kD gelatinase/type IV collagenase) in 46 patients who had neck surgery for oral cancer was studied immunohistochemically. In 20 of 26 patients (76.9%)...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/99.1.18

    authors: Kusukawa J,Sasaguri Y,Shima I,Kameyama T,Morimatsu M

    更新日期:1993-01-01 00:00:00

  • BRAF mutation status in gastrointestinal stromal tumors.

    abstract::Gastrointestinal stromal tumors (GISTs) are mesenchymal tumors characterized by mutations of KIT or PDGFRA. The objectives of this study were to evaluate BRAF mutations in GISTs and then to correlate BRAF mutational status in the tumor with clinical parameters, with B-raf expression, and with activation of some cellul...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPPCKGA2QGBJ1R

    authors: Hostein I,Faur N,Primois C,Boury F,Denard J,Emile JF,Bringuier PP,Scoazec JY,Coindre JM

    更新日期:2010-01-01 00:00:00

  • Pseudo-Pelger-Huët anomaly induced by medications: a clinicopathologic study in comparison with myelodysplastic syndrome-related pseudo-Pelger-Huët anomaly.

    abstract::Pseudo-Pelger-Huët anomaly (PPHA) has been documented in association with transplant medications and other drugs. This iatrogenic neutrophilic dysplasia is reversible with cessation or adjustment of medications but is frequently confused with myelodysplastic syndrome (MDS) based on the conventional concept that PPHA i...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPVFY95MAOBKRS

    authors: Wang E,Boswell E,Siddiqi I,Lu CM,Sebastian S,Rehder C,Huang Q

    更新日期:2011-02-01 00:00:00

  • Immunochemical detection of human blood in feces.

    abstract::Current methods for testing stool samples for hemoglobin utilize peroxidase oxidation of chemical indicators such as guaiac or benzidine. These tests have frequent false-positive and false-negative results, complicating random screening for occult gastrointestinal bleeding. The authors have developed an immunochemical...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/69.1.342

    authors: Barrows GH,Burton RM,Jarrett DD,Russell GG,Alford MD,Songster CL

    更新日期:1978-03-01 00:00:00

  • Elevation of myeloperoxidase in conjunction with cardiac-specific markers after marathon running.

    abstract::Cardiac-related death has been reported following strenuous exercise, and biochemical markers predicting adverse outcomes would be useful. Despite the fact the myeloperoxidase (MPO) release may precede myocardial injury and identify at-risk patients earlier than traditional markers, information on the effects of marat...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/1D62H6KRFTVQRJ0A

    authors: Melanson SE,Green SM,Wood MJ,Neilan TG,Lewandrowski EL

    更新日期:2006-12-01 00:00:00

  • A morphologic and cytochemical study of acute myelomonocytic leukemia with abnormal marrow eosinophils associated with inv(16)(p13q22).

    abstract::Nineteen patients with acute nonlymphocytic leukemia were found to have a pericentric inversion of chromosome 16. All were classified as having acute myelomonocytic leukemia (M4) according to French-American-British Cooperative Group (FAB) criteria. Consistent abnormalities of marrow eosinophils were present. In 15 of...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.6.733

    authors: Bitter MA,Le Beau MM,Larson RA,Rosner MC,Golomb HM,Rowley JD,Vardiman JW

    更新日期:1984-06-01 00:00:00