Abstract：
OBJECTIVES:Sclerosing pneumocytomas are rare pulmonary neoplasms that are typically benign. However, rare patients experience progressive disease, and therapy targeting specific genetic underpinnings could be an attractive therapeutic option. Recent studies have found recurrent AKT 1 mutations in sclerosing pneumocytoma, but little is known about whether oncogenic fusion genes may also be present. METHODS:To better understand the genetic background, 10 sclerosing pneumocytomas were subjected to next-generation sequencing cancer mutation panel testing (n = 9) and/or RNA sequencing (n = 3). The patients were all women (average age, 47 years; range, 17-74 years). RESULTS:Eight patients had solitary sclerosing pneumocytomas, while one had two tumors, and one had many bilateral tumors. Recurrent mutations were noted in genes involved in the mTOR pathway, including AKT1, PIK3R1, and PTEN. AKT1 alterations were particularly common, present in 78%. No recurrent genetic fusions were identified. The patient in our study with multiple bilateral lesions was treated with the mammalian target of rapamycin (mTOR) inhibitor everolimus, with no objective radiographic evidence of treatment response after 4 months. CONCLUSIONS:Our data further support that abnormal activation of the mTOR pathway is a consistent genetic event in sclerosing pneumocytoma. This warrants further exploration to determine if mTOR pathway inhibitors may be effective in patients with metastatic or recurrent disease.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Boland JM,Lee HE,Barr Fritcher EG,Voss JS,Jessen E,Davila JI,Kipp BR,Graham RP,Maleszewski JJ,Yi ESdoi
10.1093/ajcp/aqaa136subject
Has Abstractpub_date
2020-11-04 00:00:00eissn
0002-9173issn
1943-7722pii
5955429pub_type
杂志文章abstract：:Urine cultures represent 40-50% of specimens processed by hospital clinical microbiology laboratories. An accurate rapid screening method to detect positive urine cultures could greatly improve laboratory efficiency. Three hundred eighty-two unselected urines were screened by nephelometry at 2, 4, and 5 hours of shaki...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/77.3.305
更新日期：1982-03-01 00:00:00
abstract：:Analysis of the data for the 1969 to 1973 CAP Surveys of proficiency in partial thromboplastin time (PTT) determination indicates more than desirable variability in this measurement. Non-activated procedures show greater interlaboratory variability than activated methods; therefore, they may be preferable for routine ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:Evaluation of blood cells to determine immunologic status is becoming an important clinical application of flow cytometric analysis. For a wider use of immunophenotyping technology in clinical laboratories, the authors developed a rapid method to detect monoclonal antibody-labeled cells using forward light scatter/abs...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/97.3.331
更新日期：1992-03-01 00:00:00
abstract：:Fine-needle aspiration (FNA) of thyroid bed (TB) lesions is a common diagnostic modality in monitoring patients for recurrent cancer after a thyroidectomy. To elucidate the value of TB FNA, we reviewed our experience at The Johns Hopkins Hospital, Baltimore, MD. We identified 57 TB FNA specimens from 50 patients. Of t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPD60AHRUCMDPF
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVES:Promotion of high-quality care at a lower cost requires educational initiatives across the continuum of medical education. A needs assessment was performed to inform the design of an educational tool with the goal of teaching laboratory stewardship to medical students. METHODS:The needs assessment consisted...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz102
更新日期：2020-01-01 00:00:00
abstract：:Although prednisolone is the most common immunosuppressive agent used in renal transplantation, an accurate formulation of the optimal dose regimen remains to be established. A sensitive and precise radioimmunoassay was developed for this purpose. Fifty microliters of 1:20 dilutions of serum or 1:200 dilutions of urin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.6.804
更新日期：1980-06-01 00:00:00
abstract：:This report describes the selective staining of lysosomes in cells of neutrophilic granulocytic origin by the azo textile dye basic blue 93. Used as a single agent aqueous stain after fixation, the dye stained lysosomes black. Stained in this way, lysosomes were sharply delimited and easily visualized. Large numbers o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.2.195
更新日期：1988-02-01 00:00:00
abstract：:Malignant mesothelioma is increasing in incidence worldwide, including in Japan. However, the accurate pathologic diagnosis of pleural or peritoneal mesothelioma (PM) is sometimes difficult if adequate histologic and immunohistochemical analyses are not undertaken. The aim of this study was to identify a useful antibo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPHSUJ15NEYMIP
更新日期：2008-11-01 00:00:00
abstract：:This report describes four siblings affected with familial intrahepatic cholestasis detected in early infancy. In the two male siblings, biliary cirrhosis and fatal hepatocellular carcinoma later developed, whereas the female siblings have had persistent hepatomegaly and recurrent episodes of cholestasis. Sequential b...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.2.172
更新日期：1981-08-01 00:00:00
abstract：:The measurement of beta-thromboglobulin (BTG) and platelet lactic dehydrogenase (LDH) as markers for alpha-granule release and platelet lysis, respectively, has been reported to correlate with the amount of damage incurred during the preparation and storage of platelet concentrates. We compared the concentrations of t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.6.719
更新日期：1983-06-01 00:00:00
abstract：Objectives:According to current guidelines, either immunohistochemistry (IHC) or in situ hybridization (ISH) can be used to determine human epidermal growth factor receptor 2 (Her2/neu) status in breast carcinoma. While the guidelines explicitly delineate result interpretation, there is no consensus on the most appropr...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw224
更新日期：2017-04-01 00:00:00
abstract：OBJECTIVES:To describe consecutive vulvar biopsy cases and to create an educational template for pathology trainees and practicing pathologists. METHODS:We reviewed 189 consecutive biopsies from the female genital area skin and mucosa. We classified them based on etiologies and examined limited clinical information. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa135
更新日期：2020-09-11 00:00:00
abstract：:The case of a 23-year-old woman with a primary small cell carcinoma of the endometrium is reported. The light microscopic features were similar to those of small cell carcinoma of the lung and those of the uterine cervix. Electron microscopy revealed cytoplasmic neurosecretory type granules and indistinct cell junctio...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.1.98
更新日期：1984-01-01 00:00:00
abstract：:Primary angiosarcomas of the uterus are exceedingly rare. The authors report a unique case of this entity arising in connection with uterine leiomyomas. The malignant tumor cells showed focally epithelioid features and were immunoreactive for keratin. Electron microscopy and immunohistochemistry confirmed their endoth...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.5.514
更新日期：1993-11-01 00:00:00
abstract：:Diarrheal diseases caused by bacterial pathogens are important causes of morbidity in the United States, and considerable laboratory resources are spent to detect these enteric pathogens. This article reviews recent developments in the detection and identification of Campylobacter spp, enterohemorrhagic Escherichia co...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1994-04-01 00:00:00
abstract：:Mechanical release of single cell suspensions from solid tumors for DNA analysis by flow cytometry has been shown to be optimal for adenocarcinomas in general. In the breast, many adenocarcinomas are fibrotic or scirrhous, creating difficulty in separating the neoplastic cells from the stroma. The authors have conduct...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.1.8
更新日期：1995-01-01 00:00:00
abstract：:A case of signet ring cell lymphoma, a rare variant of follicular center cell lymphoma, is presented with a review of 20 other such lymphomas previously reported in the English literature. The histologic, immunocytologic, and electron microscopic features are described with speculation as to the possible relationship ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.3.358
更新日期：1984-03-01 00:00:00
abstract：:This review summarizes our experience using blinded review as a method to measure quality in surgical pathology. It details the specifics about how the review is performed, the weaknesses in the method, and then summarizes our results so far. These results suggest that error rates correlate with the individual patholo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/5KF89P63F4F6EUHB
更新日期：2007-01-01 00:00:00
abstract：:A patient with acquired von Willebrand disease associated with multiple myeloma (IgG-lambda) is described. Mixture of his plasma or IgG fraction with washed control platelets resulted in the inhibition of aggregation with ristocetin, but mixture of control plasma or IgG fraction with washed patient platelets showed no...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/87.5.663
更新日期：1987-05-01 00:00:00
abstract：:The authors describe a patient with large granular lymphocytosis who presented with fever of unknown origin and jaundice. Immunophenotyping showed that most of the large granular lymphocytes (LGLs) were CD3-, CD16-, and NKH-1 (Leu-19)+ lymphocytes. Lymphocytosis of this subset of LGLs has not been reported. Analysis o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.2.211
更新日期：1990-08-01 00:00:00
abstract：OBJECTIVES:To describe the histopathologic and immunophenotypic features of a temporary splenic allograft exposed to massive donor-specific antibody (DSA) insult. METHODS:A human cadaveric donor splenic allograft was temporarily transplanted in a highly sensitized patient with the intention of removing DSA before inte...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa175
更新日期：2020-11-21 00:00:00
abstract：:This report describes the use of counterimmunoelectrophoresis (CIE) as a method of detecting activation of the complement system. With this technic small amounts of C3 split products (C3c/d) can be detected in plasma samples by specific precipitation with antiserum in less than two hours. The CIE technic is a highly s...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.4.440
更新日期：1978-04-01 00:00:00
abstract：:We compared the morphologic findings of different types of marginal zone B-cell lymphoma (MZL) involving the bone marrow (BM), including 18 splenic (SMZL), 6 extranodal (mucosa-associated lymphoid tissue lymphoma), and 6 nodal cases. The median percentage of BM involvement was 15%, and multiple overlapping patterns of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/HRHQFBFTR8B4LXT4
更新日期：2008-05-01 00:00:00
abstract：:Conventional cytogenetic studies have suggested that trisomy 12 may be a characteristic nonrandom numerical chromosome anomaly in benign ovarian tumors, particularly sex cord-stromal tumors. To confirm this finding, and to avoid possible culture artifact introduced during cytogenetic analysis, the authors performed fl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/102.6.775
更新日期：1994-12-01 00:00:00
abstract：:Screening sera for streptococcal antibodies is a useful clinical procedure when recent streptococcal infection requires verification. Presently there are two test kits available that detect five (or more) of the antibodies that may be present. These two screening tests were compared using antistreptolysin O (ASO) and ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.2.218
更新日期：1979-08-01 00:00:00
abstract：:It is said that HER2/neu expression by immunohistochemical analysis varies with the time of fixation. The purpose of this pilot study was to determine the impact of the length of fixation in 10% buffered formalin on the expression of HER2/neu by immunohistochemical analysis. We studied tissue samples from 10 invasive ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPAIJPSN4A9MJI
更新日期：2010-10-01 00:00:00
abstract：:The overall performances of several enzyme reagent kits for alkaline phosphatase, creatine kinase, lactic dehydrogenase, and aspartate aminotransferase were evaluated using an ABA-100 Bichromatic Analyzer. Interassay precision using this instrument with commercial reagents compared well with published data for similar...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.2.151
更新日期：1979-08-01 00:00:00
abstract：:The probabilities of low transferrin saturation, folic acid, or vitamin B12 levels in association with various erythrocyte indices was determined from the prevalences of these abnormalities and the distributions of the indices among 206 hospitalized and 1,000 ambulatory anemic patients. At mean corpuscular hemoglobin ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.5.748
更新日期：1978-11-01 00:00:00
abstract：OBJECTIVES:Our aim was to investigate the frequency of steatohepatitic morphology in hepatocellular adenoma (HCA) and correlate with its clinical parameters and risk factors underlying nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH). METHODS:We examined a series of 41 liver resection sp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa075
更新日期：2020-09-08 00:00:00
abstract：:This paper's purpose is to document a case of unilateral tonsillar malakoplakia. Escherichia coli was repeatedly cultured from the lesion, and the isolate was studied by expert microbiologists who found no abnormalities in its biologic properties. The course of the lesion was followed by repeated punch biopsies before...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.3.390
更新日期：1982-09-01 00:00:00
