Abstract:
Objectives:According to current guidelines, either immunohistochemistry (IHC) or in situ hybridization (ISH) can be used to determine human epidermal growth factor receptor 2 (Her2/neu) status in breast carcinoma. While the guidelines explicitly delineate result interpretation, there is no consensus on the most appropriate testing algorithm. Methods:The Her2/neu statuses of 369 consecutive cases of invasive breast cancer (from 351 patients) were assessed in a dual-testing algorithm that uses both IHC and fluorescence ISH (FISH). FISH was performed using dual-color HER2/ chromosome enumeration probe 17 ( CEP17 ) probes, and if equivocal results were obtained, reflex testing using HER2/lissencephaly gene 1 ( LIS1 ) probes was used. Results from both modalities were scored and reported using American Society of Clinical Oncology/College of American Pathologists 2013 criteria. Results:Sixty-one (16.5%) of the 369 tumors were found to be Her2/neu positive by at least one modality. The overall concordance between IHC and FISH results was 97.6%. Six of the 369 tumors were reclassified as Her2/neu positive after a negative IHC result. FISH was also able to identify significantly more Her2/neu-positive cases than IHC. Conclusions:The commonly used reflex strategy based on IHC results may deny potentially beneficial targeted therapy for a small cohort of patients, which should be considered as testing guidelines are formulated and the cost-benefit analyses of various testing algorithms are assessed.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Solomon JP,Dell'Aquila M,Fadare O,Hasteh Fdoi
10.1093/ajcp/aqw224subject
Has Abstractpub_date
2017-04-01 00:00:00pages
432-437issue
4eissn
0002-9173issn
1943-7722pii
3066377journal_volume
147pub_type
杂志文章abstract::Fine-needle aspiration (FNA) is the standard of care for the initial workup of thyroid nodules, but there is no consensus algorithm to manage patients with benign results. We examined performance characteristics of initial and repeat satisfactory FNAs for all 402 patients who underwent thyroid surgery during a recent ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4AXL-DMN1-JRPM-TX5P
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVES:To determine the current state of pathology resident training in genomic and molecular pathology. METHODS:The Training Residents in Genomics (TRIG) Working Group developed survey and knowledge questions for the 2013 pathology Resident In-Service Examination (RISE). Sixteen demographic questions related to a...
journal_title:American journal of clinical pathology
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更新日期:2014-10-01 00:00:00
abstract:OBJECTIVES:The 2014 Bethesda System (TBS 2014) guidelines for reporting cervical cytology revised the age for reporting benign endometrial cells (BECs) from 40 years or older to age 45 years or older. We evaluated this change and further investigated if extending the reporting age to 50 years or older may be acceptable...
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abstract::The authors studied the effect of averaging replicated assays of Factor IX coagulation activity and Factor IX antigen on each plasma specimen in improving the ability to detect carriers of hemophilia B. The improvement resulting from this procedure is particularly marked in tests depending on the linear regression of ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/72.6.1000
更新日期:1979-12-01 00:00:00
abstract::Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...
journal_title:American journal of clinical pathology
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更新日期:2008-07-01 00:00:00
abstract::Hemizygous TP53 gene deletion is the most important adverse risk factor in chronic lymphocytic leukemia (CLL), but its relationship with p53 protein expression is unclear. We investigated 110 CLL cases and correlated nuclear p53 protein immunoreactivity with TP53 gene deletion status and other CLL-associated genetic r...
journal_title:American journal of clinical pathology
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更新日期:2010-01-01 00:00:00
abstract::A 73-year-old man with myelodysplastic syndrome developed during the course of his illness auto-immune hemolytic anemia. The association between auto-immune hemolysis and myeloproliferative disease is extremely rare. An even more unique finding is the nature of the antibody which demonstrated single specificity agains...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/77.3.356
更新日期:1982-03-01 00:00:00
abstract:OBJECTIVES:To differentiate the leprosy agents Mycobacterium leprae and Mycobacterium lepromatosis and correlate them with geographic distribution and clinicopathologic features. METHODS:Species-specific polymerase chain reactions were used to detect each bacillus in archived skin biopsy specimens from patients with l...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP1GLCBE5CDZRM
更新日期:2014-10-01 00:00:00
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更新日期:1980-10-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1991-05-01 00:00:00
abstract::The expression of the cell-cycle-associated Ki-67 antigen by MIB-1 monoclonal antibody was retrospectively assessed in 35 surgically resected neuroendocrine tumor specimens of the pancreas embedded in paraffin. The MIB-1 proliferation index was correlated with the classification of the neuroendocrine tumors of the pan...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
abstract::While plasmacytosis is a common occurrence in early post-bone marrow transplantation biopsy specimens, the significance of plasma cells in such specimens from patients with multiple myeloma (MM) is unknown. We attempted to retrospectively determine, by morphologic assessment of plasma cell percentage, immunohistologic...
journal_title:American journal of clinical pathology
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更新日期:1997-06-01 00:00:00
abstract::Fungal organisms morphologically resembling Candida were found in one third of 72 consecutive surgically resected gastric ulcers. In over half of these cases the organisms were present in the mycelial form. Large numbers of fungi forming clusters were found in 13 cases (18%) and were associated with a surprisingly hig...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
abstract::Two commercial enzyme-linked immunosorbent assays (ELISA) for detection of herpes simplex virus (HSV) antigen in direct clinical specimens were compared with cell culture in primary rabbit kidney and MRC-5. With a total of 238 specimens, the sensitivity and specificity of the Dako ELISA method were determined to be 70...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.2.229
更新日期:1986-02-01 00:00:00
abstract::A fatal case of an apparently congenital form of hemophagocytic reticulosis is reported. The onset was manifested by hyperbilirubinemia and hepatosplenomegaly which were present at birth and persisted throughout life. Fever, anemia and pancytopenia developed at 1 month of age and became progressively worse. A splenect...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.4.495
更新日期:1976-04-01 00:00:00
abstract::The authors have examined the cellular distribution of lipocortin-1 (L-1) in the normal and diseased central nervous system (CNS) using the peroxidase-antiperoxidase (PAP) technique with a polyclonal antibody specific for L-1. L-1 immunoreactivity was evaluated in the frontal cortex, parahippocampal gyrus/lateral vent...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/92.4.424
更新日期:1989-10-01 00:00:00
abstract::The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.6.687
更新日期:1990-12-01 00:00:00
abstract::Acute exacerbations of idiopathic pulmonary fibrosis/cryptogenic fibrosing alveolitis (IPF/CFA) are rare and typically terminal events, but their relationship to underlying patterns of idiopathic interstitial pneumonias is unknown. We reviewed autopsy material from patients who died of diffuse alveolar damage in the c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/UVAR-MDY8-FE9F-JDKU
更新日期:2003-05-01 00:00:00
abstract:OBJECTIVES:To evaluate the impact of implementing the dual interpretation of atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and low-grade squamous intraepithelial lesion (LSIL) after the Bethesda System 2014 and to compare it with other indeterminate interpretations. METHODS...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa069
更新日期:2020-09-08 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2012-05-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1987-01-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
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更新日期:2011-12-01 00:00:00
abstract::Hepatocytes with orcein-positive ground-glass cytoplasm have been shown to contain abundant hepatitis B surface antigen in the cisternae of excess smooth endoplasmic reticulum. Hepatocytes with similar cytoplasmic changes that did not react with orcein were observed in 26.5% of 49 cases of cirrhosis. These cells exhib...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.4.498
更新日期:1981-04-01 00:00:00
abstract::The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1990-04-01 00:00:00
abstract::A simple assay based on molecular light scattering was developed to detect soluble immune complexes. In this assay the percentage of relative light scattering (%RLS) caused by complexes in buffer containing polyethylene glycol 6000 (PEG) was measured with a laser nephelometer. In low concentrations of PEG (3%-5%), IgG...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.6.804
更新日期:1980-12-01 00:00:00
abstract:OBJECTIVES:Many nonpathologists and some pathologists consider utilization review essential to laboratory quality improvement, but (1) confusion surrounding the definition of "appropriate" laboratory utilization, (2) the reliance on manual chart review, and (3) a lack of leadership have contributed to its unstandardize...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
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更新日期:2014-06-01 00:00:00
abstract::Mycobacteriology results of the CAP proficiency testing programs are reviewed for the years 1975-1981. Survey participants have shown a continuing and improving ability to isolate and identify Mycobacterium tuberculosis, nontuberculous mycobacteria, and to identify acid-fast bacteria in stain preparations. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Tissue repositories maintained by pathology departments represent an abundant resource of clinically annotated human specimens. The storage expenses associated with pathology archives are known to administrators of most pathology departments. However, such basic repository characteristics as the quality of stored mate...
journal_title:American journal of clinical pathology
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更新日期:2011-05-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
abstract::Venous thromboembolism (VTE) results from the interaction of the Virchow triad (venous stasis, endothelial injury, and hypercoagulability). Risk factors for increased hypercoagulability, or thrombophilia, include activated protein C resistance/factor V Leiden, the prothrombin G20210A mutation, deficiencies of the natu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP5SQT3ZKYQFBM
更新日期:2012-04-01 00:00:00