Abstract：
:This report describes the selective staining of lysosomes in cells of neutrophilic granulocytic origin by the azo textile dye basic blue 93. Used as a single agent aqueous stain after fixation, the dye stained lysosomes black. Stained in this way, lysosomes were sharply delimited and easily visualized. Large numbers of black-stained lysosomes occurred in immature granulocytes like promyelocytes and myelocytes. In more mature granulocytes like metamyelocytes, bands, and neutrophils, substantially fewer numbers of lysosomes were detected. In leukemic myeloblasts and promyelocytes stained with basic blue 93, variable numbers of black-colored lysosomes could be detected and usually paralleled the reactions for myeloperoxidase and Sudan black B in the same cells. As an alternative to the traditional stains for myeloperoxidase, basic blue 93 has several advantages, including simplicity of use, ability to stain aged specimens, and stability of the reaction product.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Kass Ldoi
10.1093/ajcp/89.2.195subject
Has Abstractpub_date
1988-02-01 00:00:00pages
195-202issue
2eissn
0002-9173issn
1943-7722journal_volume
89pub_type
杂志文章abstract：:Reduction mammaplasty (RM) is a common surgical procedure that yields a variable amount of tissue for pathologic examination. Occult breast carcinomas are detected rarely in these specimens. We evaluated the pathologic findings in RM specimens performed in our institution during an 11.5-year period (July 1989 to Decem...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/4kd652hn739xtlm3
更新日期：2003-09-01 00:00:00
abstract：:The objective of the study was to evaluate the current standard practice of using volume and total nucleated cell (TNC) count for the selection of cord blood (CB) units for cryopreservation and further transplantation. Data on 794 CB units whose CD34+ cell content was determined by flow cytometry were analyzed by usin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPFB6EXO7BJVLR
更新日期：2011-11-01 00:00:00
abstract：:Data from the 1977 College of American Pathologists (CAP) Comprehensive Chemistry Survey and other programs have been analyzed in an interlaboratory comparison of the measurements of serum uric acid. The majority of clinical laboratories utilize some form of phosphotungstic acid (PTA) oxidation-reduction technic for t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Acute paraplegia developed in a 53-year-old man with pulmonary aspergilloma because of contiguous extension of Aspergillus infection to the epidural and subdural spaces and spinal cord. Histopathologic findings of the spinal cord showed Aspergillus hyphae penetrating the myelin sheath and myelomalacia, predominantly i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.6.763
更新日期：1985-12-01 00:00:00
abstract：:The radioimmunoassay of ACTH was used in a routine laboratory to localize the site of the lesion in 20 patients with Cushing's syndrome. Eight of the patients had no detectable circulating ACTH and had adrenal tumors removed, 12 had high levels and were diagnosed as having pituitary Cushing's syndrome. Very high level...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.5.618
更新日期：1975-11-01 00:00:00
abstract：:Counterimmunoelectrophoresis was used to detect Cryptococcus neoformans capsular polysaccharide in the body fluids of infected patients. Using rabbit anti-C. neoformans A15 globulin, counterimmunoelectrophoresis detected 1.25 microgram of purified cryptococcal polysaccharide per ml of cerebrospinal fluid (CSF) or urin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.1.39
更新日期：1977-07-01 00:00:00
abstract：:Antibody to hepatitis B core antigen (anti-HBc) has previously been recognized to be a sensitive marker of hepatitis B virus (HBV) infection. In addition, anti-HBc has recently been suggested to be a surrogate marker for non-A, non-B hepatitis agents in donated blood. The authors studied prospectively the HBV antigen ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.1.79
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVES:An intraoperative consultation (IOC) checklist was developed and implemented aimed at standardizing slide labeling and monitoring metrics central to quality and safety in surgical pathology. DESIGN:Data were collected for all IOC cases over a 9-month period. Slide labeling defect rates and IOC turnaround ti...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz009
更新日期：2019-05-03 00:00:00
abstract：OBJECTIVES:Our aim was to investigate the frequency of steatohepatitic morphology in hepatocellular adenoma (HCA) and correlate with its clinical parameters and risk factors underlying nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH). METHODS:We examined a series of 41 liver resection sp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa075
更新日期：2020-09-08 00:00:00
abstract：:We report serum enzyme patterns in three patients with polymyositis or dermatomyositis whose cases posed potential problems in distinguishing malignancy, hepatocellular damage, and myocardial infarction from myositis. The alanine aminotransferase showed five- to 16-fold elevations. The creatine kinase MB isoenzyme and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.4.556
更新日期：1980-04-01 00:00:00
abstract：:Cytokeratin (CK) expression was studied in 22 sinonasal inverted papillomas. Columnar (respiratory) epithelium in inverted papillomas abundantly expressed CK7, CK8, CK18, and CK19. Immunoreactivity for CK5/14 and CK17 was found in basal and parabasal/suprabasal cells. Transitional (cuboidal) and squamous epithelium in...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/TH5J-T1Q6-9QJR-8CDK
更新日期：2001-05-01 00:00:00
abstract：:Typical yeast-phase cells of Blastomyces dermatitidis have a characteristic appearance in tissue sections. Fungal morphologic variation occurs infrequently in the lesions of blastomycosis, yet it can complicate the differential diagnosis, particularly if fresh tissue is not available for microbiologic culture. The aut...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.4.575
更新日期：1990-04-01 00:00:00
abstract：:Histologic diagnosis of a nonpalpable breast lesion requires the following: (1) mammographically directed excision of the suspect lesion, (2) recovery of the target lesion from the tissue specimen, and (3) demonstration of the lesion in histologic section. Recovery of lesional tissue is a function of localization with...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/92.4.452
更新日期：1989-10-01 00:00:00
abstract：:Data from 2.5 million glucose analyses on pools of lyophilized human quality control serum were used to evaluate analyte stability in the prereconstitution phase. Input information was from laboratories in Regional Quality Control Programs that use CAP Quality Assurance Service (QAS) data processing. Of 31 pools in us...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:Although cytology laboratories are mandated to rescreen at least 10% of cervicovaginal smears, there is no uniform national rescreening practice. Follow-up data for 16,188 rescreened cervicovaginal smears were studied and decision analysis was performed to determine an optimal rescreening strategy. High-grade dysplasi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/111.5.601
更新日期：1999-05-01 00:00:00
abstract：:Blood glucose results for 2,601 specimens obtained at multiphasic health examination, divided into three age groups, were analyzed using probability plot paper. Two straight lines could be fitted to each distribution. The assumption was made that the lower lines correspond to "normal-healthy" persons, whereas the uppe...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.2.211
更新日期：1981-08-01 00:00:00
abstract：:In lung transplant recipients, bronchoalveolar lavage (BAL) is mainly performed to detect infectious agents. However, in addition to microorganisms, epithelial cell atypia may be identified, and determination of its significance is necessary. Specimens obtained at BAL in lung and heart-lung transplant recipients (LTRs...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/112.2.204
更新日期：1999-08-01 00:00:00
abstract：:Twenty-eight resected primary non-small cell lung carcinomas were studied for intratumoral DNA variability by flow cytometry (FCM). Three separate tissue specimens from each resected tumor were divided equally for FCM analysis and histologic evaluation. FCM analysis also was performed on fine-needle aspirates (FNAs) f...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.3.311
更新日期：1991-09-01 00:00:00
abstract：:Adenomatous polyps are common neoplastic lesions of the large intestine. The risk of carcinoma increases with polyp size. Small polyps are typically totally embedded for histologic examination, but no standard method for sampling large, grossly benign polyps has been established. We reviewed grossly noninvasive adenom...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/804U-LHU1-TJ00-UGAU
更新日期：2001-09-01 00:00:00
abstract：:The authors evaluated the Yellow IRIS automated urinalysis instrument (International Remote Imaging Systems, Chatsworth, CA 91311) in terms of its analytic performance as well as its ease of use. The Yellow IRIS is a system using automated intelligence microscopy (AIM), coupled with a dipstick reader and specific grav...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.5.661
更新日期：1986-11-01 00:00:00
abstract：:Proper collection, handling, and storage of the blood alcohol specimen are essential in medicolegal cases involving the question of sobriety. A standard operating procedure is necessary to ensure maximum reliability. Comments are offered on the advantages of using blood specimens in preference to urine or tissue speci...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/74.5.743
更新日期：1980-11-01 00:00:00
abstract：:The purpose of this study was to correlate p53 gene alterations and their expression in 85 head and neck squamous cell carcinomas. Genomic p53 was amplified with the polymerase chain reaction (PCR) from formalin-fixed, paraffin-embedded tissues. Exons 5 through 8 were screened for mutations by means of non-isotopic si...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.1.7
更新日期：1997-01-01 00:00:00
abstract：:The authors report two cases of vernix caseosa peritonitis, an infrequent complication of cesarean section with distinctive histopathologic findings. Both patients underwent exploratory laparotomy for unexplained abdominal pain after cesarean section. Histopathologic evaluation of surgically removed tissue revealed an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.6.681
更新日期：1995-06-01 00:00:00
abstract：:S-100 protein was detected in a small number of human peripheral T-lymphocytes by a direct immunoperoxidase method with the use of monospecific antibody to S-100 protein. Complemented-mediated lysis using monoclonal antibodies revealed that the S-100+ T-lymphocytes bore OKT3, OKT8, and OKT11 antigens but not OKT4, OKM...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/83.1.69
更新日期：1985-01-01 00:00:00
abstract：:Fixed lymphoma tissues from 11 patients seropositive for human T-cell leukemia virus type I (HTLV-I) antibodies were analyzed for proviral sequences by the polymerase chain reaction. Typical adult T-cell leukemia/lymphomas (ATLLs) from nine patients were positive for HTLV-I sequences. In contrast, one of two lymphomas...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.4.536
更新日期：1991-04-01 00:00:00
abstract：:Two hundred serum samples were tested with two assays for rapid detection of cytomegalovirus (CMV) antibodies. The assays were CMV Scan, a two-step latex particle agglutination assay for CMV IgG and IgM antibodies, and TranSTAT-CMV, a newly available six-step solid-phase enzyme immunoassay for CMV IgG antibodies. Comp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.5.539
更新日期：1993-05-01 00:00:00
abstract：:Specific esterase activity, a property generally ascribed to cells of granulocytic origin, was found in abnormal erythroid precursors obtained from the bone marrows of six patients with untreated erythroleukemia. These erythroblasts also showed nonspecific esterase activity. Neither specific nor nonspecific esterase a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/67.4.368
更新日期：1977-04-01 00:00:00
abstract：:RhD determination in pregnant women is critical to facilitate Rh immune globulin prophylaxis for RhD-negative women. A single amino acid change in the RhD antigen can cause epitope loss, giving rise to "partial D" variants. Women with some partial D variants may develop anti-D against the missing epitope after pregnan...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPSXN9HQ4DELJE
更新日期：2010-09-01 00:00:00
abstract：:We sought to characterize how well computerized "case flags" performed in evaluating critical diagnoses in anatomic pathology. All cases identified by a pathologist at sign-out and flagged as a critical diagnosis in anatomic pathology in 2 hospital laboratories during a 3-year period were reviewed. A subset of all cri...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPL1N5KBAFFDHV
更新日期：2012-03-01 00:00:00
abstract：:This paper's purpose is to document a case of unilateral tonsillar malakoplakia. Escherichia coli was repeatedly cultured from the lesion, and the isolate was studied by expert microbiologists who found no abnormalities in its biologic properties. The course of the lesion was followed by repeated punch biopsies before...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.3.390
更新日期：1982-09-01 00:00:00
