Abstract：
:The purpose of this study was to correlate p53 gene alterations and their expression in 85 head and neck squamous cell carcinomas. Genomic p53 was amplified with the polymerase chain reaction (PCR) from formalin-fixed, paraffin-embedded tissues. Exons 5 through 8 were screened for mutations by means of non-isotopic single-strand conformation polymorphism (SSCP) analysis. p53 expression was detected by means of immunohistochemistry (IHC) with the p53 monoclonal antibody DO-7. Twenty-three lesions (27%) showed both SSCP variants and DO-7 immunostaining, whereas 37 (44%) demonstrated both SSCP normal patterns and negative staining. Twenty-five lesions (29%) were discordant, including 12 IHC-positive (14%) and 13 SSCP-positive (15%) lesions. However, discordant IHC-negative, SSCP-positive lesions could have been easily interpreted after sequencing of the abnormal samples. Had these been screened with IHC only, all nonsense or frameshift p53 mutations would have been missed. IHC-positive, SSCP-negative lesions were interpreted in light of current models of p53 immunodetection. Had these been screened with SSCP or sequencing only, a sizeable number of tumors expressing p53 protein abnormalities would have been undetected. Therefore, simultaneous use of both methods increases the number of p53 abnormalities detected, and is warranted.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Calzolari A,Chiarelli I,Bianchi S,Messerini L,Gallo O,Porfirio B,Mattiuz PLdoi
10.1093/ajcp/107.1.7subject
Has Abstractpub_date
1997-01-01 00:00:00pages
7-11issue
1eissn
0002-9173issn
1943-7722journal_volume
107pub_type
杂志文章abstract：:Screening sera for streptococcal antibodies is a useful clinical procedure when recent streptococcal infection requires verification. Presently there are two test kits available that detect five (or more) of the antibodies that may be present. These two screening tests were compared using antistreptolysin O (ASO) and ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.2.218
更新日期：1979-08-01 00:00:00
abstract：:The blood counts and lactic dehydrogenase values of eight patients with thrombotic thrombocytopenic purpura (TTP) were reviewed in relation to the clinical course. Three of the eight patients died. In these patients, the hemoglobin was significantly lower and the LDH higher at the time of presentation than that of the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.5.700
更新日期：1983-11-01 00:00:00
abstract：:FNAB is the most important advance in the management of thyroid nodules in the past two decades. Dr Mazzaferri has said that finding a thyroid cancer in a thyroid nodule is like finding a needle in a haystack. Fine-needle aspiration biopsy is like applying a strong electromagnet to find that needle. It should be avail...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1997-10-01 00:00:00
abstract：:Twenty cases of inflammatory myofibroblastic tumor (IMT) were studied; 19 involved the lung and 1 the esophagus only. The patients' ages ranged from 3 to 72 years. There were 9 males and 11 females. Involvement of a bronchus was seen in one case and of mediastinal structures in four. Chest pain and dyspnea were common...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.5.538
更新日期：1990-11-01 00:00:00
abstract：:Measuring HER-2 is important for selecting optimal therapy and predicting prognosis in breast cancer patients. Current methods for evaluating HER-2 include measuring protein overexpression by immunohistochemistry (IHC), measuring gene copy number by fluorescent in situ hybridization (FISH), and measuring shed antigen ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/D76M-QEGU-62BT-HKER
更新日期：2002-06-01 00:00:00
abstract：:For quality-control purposes, federal regulations require cytology laboratories to compare Papanicolaou smear and cervical biopsy reports, if available, and determine the cause of any discrepancies. The authors reviewed 56,497 cervicovaginal smears, of which 1,582 (2.8%) had a subsequent cervical biopsy or endocervica...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.1.32
更新日期：1995-01-01 00:00:00
abstract：:The association between Campylobacter-like organisms (CLOs) and lesions of the gastric mucosa was studied in 59 consecutive biopsies. Hematoxylin and eosin and Warthin-Starry silver stains, as well as high-resolution light microscopy (HRLM) and transmission electron microscopy (TEM), were used. The organisms were foun...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.5.575
更新日期：1986-11-01 00:00:00
abstract：:Utilization of nonconventional bacterial strains and electron microscopy occasionally may aid in the recognition of unusual microorganisms which fail to be demonstrated by more conventional bacterial stains. We report an unusual case of recurrent vaginal polyps, initially thought by physical examination to represent a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.5.637
更新日期：1983-05-01 00:00:00
abstract：:This article explores 3 aspects of breast diagnosis that are currently under investigation and about which our thinking has recently undergone considerable reshaping. The trend toward more frequent evaluation of clinically subtle lesions has suggested that it might be necessary to understand thefine-needle aspiration ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/29FP-0QAU-CMJY-BK3U
更新日期：2000-05-01 00:00:00
abstract：OBJECTIVES:Mucinous cystic neoplasm of the liver is characterized by neoplastic mucinous and/or biliary epithelium surrounded by ovarian-type stroma. Immunohistochemical studies have shown that the ovarian-type stroma expresses estrogen receptor, suggesting potential hormonal responsiveness. The molecular biology of mu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa115
更新日期：2020-11-04 00:00:00
abstract：:Primary gastric non-Hodgkin lymphoma of mucosa-associated lymphoid tissue (MALT-NHL) is strongly related to Helicobacter pylori (H pylori) infection. CagA positive H pylori strains appear to be more aggressive, as reflected by a greater degree of gastric inflammation and higher levels of Il-8 production. Using a polym...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/106.5.670
更新日期：1996-11-01 00:00:00
abstract：:A primary hepatic tumor with histologic features suggestive of carcinoid tumor is reported, and six previously reported cases reviewed. Tumor cells contained electron dense secretory granules with an average diameter 150-200 nm which demonstrated positive argyrophilic reactions. On immunohistochemistry, chromogranin A...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.4.561
更新日期：1988-04-01 00:00:00
abstract：:Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/6H9844AMMET82DBJ
更新日期：2008-07-01 00:00:00
abstract：:To assess the diagnostic accuracy of margin evaluation of melanocytic lesions using en face frozen sections compared with standard paraffin-embedded sections, we studied 2 sets of lesions in which en face frozen sections were used for analysis of surgical margins (13 from malignant melanomas [MMs] and 10 from nonmelan...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/J1Q0-V35E-UTMV-R193
更新日期：2003-08-01 00:00:00
abstract：:We determined whether there were additional diagnostic findings in additional level sections performed on polyps with no pathologic diagnosis (NPD) or those in which only lymphoid aggregates (LAs) were seen initially and determined the level at which findings were identified. All colorectal biopsy specimens submitted ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/27NQ-A1R2-DQ41-20Y7
更新日期：2002-03-01 00:00:00
abstract：:Thirty-five consecutive renal biopsy specimens were evaluated by light histology using paraffin and glycol methacrylate embedding. The clarity of the renal structures was found to be much better, and the renal lesions better defined, using glycol methyacrylate embedding. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.5.655
更新日期：1975-11-01 00:00:00
abstract：:Twenty-five cases of tubal or tubo-endometrioid metaplasia that occurred in patients from 21 to 51 years of age (mean 39 years) are described. The majority of the cases were seen in consultation. They were referred because of diagnostic problems resulting from features that have received little emphasis. The metaplasi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.5.618
更新日期：1995-05-01 00:00:00
abstract：:Estrogen receptor (ER), progesterone receptor (PR), and c-ERBB2 (HER2/neu) are therapeutically and prognostically important markers in the management of breast carcinoma. They are not always analyzed in distant metastatic and locally recurrent breast cancers. We compared immunohistochemical expression in a series of p...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPJ57FLLJRXKPV
更新日期：2010-03-01 00:00:00
abstract：:A patient with acquired von Willebrand disease associated with multiple myeloma (IgG-lambda) is described. Mixture of his plasma or IgG fraction with washed control platelets resulted in the inhibition of aggregation with ristocetin, but mixture of control plasma or IgG fraction with washed patient platelets showed no...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/87.5.663
更新日期：1987-05-01 00:00:00
abstract：:The development and general concepts of the Système International d'Unités (SI units) are discussed. The basic and derived quantities and units of the SI used for clinical laboratory data are reviewed. Ranges of normal values for a number of body fluid constituents are given in the units in current general use and in ...
journal_title：American journal of clinical pathology
pub_type： 历史文章,杂志文章
doi：10.1093/ajcp/65.1.2
更新日期：1976-01-01 00:00:00
abstract：:A murine monoclonal antibody (MAb 12C3) that is specific to human ovarian carcinomas was generated by immunizing mice with a human ovarian germinoma cell line (JOHYC-2). The antigen distribution that was defined by MAb 12C3 in normal and malignant human tissues was analyzed by immunohistochemistry on paraffin-embedded...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.3.288
更新日期：1995-03-01 00:00:00
abstract：Objectives:Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of hematolymphoid proliferations arising in the context of chronic immunosuppression. The common and indolent B-cell lymphomas, including extranodal marginal zone lymphomas (ENMZLs) of mucosa-associated lymphoid tissue (MALT), are...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/aqx134
更新日期：2017-12-20 00:00:00
abstract：:The immunochemical characterization of the light chains of the mesangial immunoglobulin A (IgA) deposits were studied in 45 patients with IgA nephropathy. Kappa and lambda light chains were detected with direct immunofluorescence (IF) method, using monospecific rabbit anti-human anti-kappa and anti-lambda anti-sera. T...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.5.548
更新日期：1986-05-01 00:00:00
abstract：:The recent clinical and commercial success of anticancer antibodies such as rituximab and trastuzumab has created great interest in antibody-based therapeutics for hematopoietic malignant neoplasms and solid tumors. Given the likelihood of lower toxic effects of antibodies that target tumor cells and have limited impa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/Y6LP-C0LR-726L-9DX9
更新日期：2003-04-01 00:00:00
abstract：:Cytopathologic smears and/or imprints of human adrenal cortex (9 cases) and its disorders were examined, including adrenocortical nodule (3 cases), adrenocortical adenoma (23 cases), carcinoma (8 cases), and renal cell carcinoma (6 cases). Immunocytochemistry directed against 3 beta-hydroxysteroid dehydrogenase and ad...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.2.161
更新日期：1995-08-01 00:00:00
abstract：:Three stillborn fetuses are reported in which an abdominal wall defect was associated with defects in the urogenital and anal region. Autopsy of these fetuses provided clues indicative of how and where the embryonic development went wrong. The malformation involved a disturbance of the cell deposition process, occurri...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.5.640
更新日期：1991-11-01 00:00:00
abstract：OBJECTIVES:GATA3 is a transcription factor regulating luminal cell differentiation in the mammary glands and has been implicated in the luminal types of breast carcinoma. The prognostic significance of GATA3 in breast cancer remains controversial. METHODS:In this study, we assessed the prognostic value of the molecule...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP5MMR1FJVVTPK
更新日期：2015-11-01 00:00:00
abstract：:A computer software package written in Dibol for a PDP 8/E computer is described that is designed for the needs of the cytology department of a moderate-sized private medical laboratory. Computerized accessioning of cases, report preparation and printing, historical filing, activity summary reports, and direct patient...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.4.421
更新日期：1979-04-01 00:00:00
abstract：:A unique case of intramural uterine tumor composed of malignant epithelium and benign smooth muscle, adipose tissue, and cartilage is reported. This tumor, which the authors designated "carcinomesenchymoma," apparently represents an example of a previously unreported variant of mixed müllerian tumor that is composed o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.5.746
更新日期：1981-05-01 00:00:00
abstract：:The performance characteristics of the XE-2100 (Sysmex, Kobe, Japan) automated immature granulocyte (IG) count were studied. The automated IG count was compared with the manual morphology count and with a proposed reference flow cytometric count. The comparison data were analyzed by both least-squares and Passing-Babl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/TVGKD5TVB7W9HHC7
更新日期：2007-09-01 00:00:00
