Abstract:
:A murine monoclonal antibody (MAb 12C3) that is specific to human ovarian carcinomas was generated by immunizing mice with a human ovarian germinoma cell line (JOHYC-2). The antigen distribution that was defined by MAb 12C3 in normal and malignant human tissues was analyzed by immunohistochemistry on paraffin-embedded and frozen sections. The antibody reacted with 67.7% (21 of 31 cases) of epithelial ovarian carcinomas (6 of 12 cases of serous cystadenocarcinoma, 5 of 7 cases of mucinous cystadenocarcinoma, 7 of 9 cases of clear cell carcinoma, 3 of 3 cases of endometrioid adenocarcinoma), but did not react with any of the benign epithelial ovarian adenomas tested. Partial regions of borderline ovarian malignancies that exhibited marked papillary projection of the lining cells with cellular atypia reacted positively with MAb 12C3 in 14 of 25 cases (56.0%). The histologic features of regional early malignant change corresponded to the expression of the MAb 12C3 epitope in the borderline malignant tumor cells. There was a low frequency of reaction (4.3%) between the antibody and other gynecologic and nongynecologic malignancies (46 cases of 12 tissues). In normal tissues, the antibody reacted positively with only three tissues, including corpora lutein cells, excretory ducts in the submandibular gland, and basal cells of the sebaceous glands. The antigen epitope defined by MAb 12C3 was present on a glycoprotein with a molecular mass of 200 kDa and did not exhibit any cross-reactivity with other well-known tumor markers. These data suggest that MAb 12C3 may be a useful tool for the immunohistologic detection of early malignant changes in epithelial ovarian tumors. In addition, MAb 12C3 also may facilitate a differential diagnosis between benign and borderline malignancies.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Yamada K,Ohkawa K,Joh Kdoi
10.1093/ajcp/103.3.288subject
Has Abstractpub_date
1995-03-01 00:00:00pages
288-94issue
3eissn
0002-9173issn
1943-7722journal_volume
103pub_type
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journal_title:American journal of clinical pathology
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abstract::An unusual case of acute myelomonocytic leukemia (AMML) in an 81-year-old man is described. Ultrastructural examination revealed the presence of distinct Auer bodies, which commonly are found in acute promyelocytic leukemia (APL). The Auer bodies had a splinterlike appearance with a distinct tubular substructure. The ...
journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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更新日期:2007-07-01 00:00:00
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journal_title:American journal of clinical pathology
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abstract::Four commercially available herpes simplex virus 1 (HSV-1) indirect fluorescent antibody (IFA) kits were compared with the use of sera selected because they were negative for HSV antibody by complement fixation (CF less than 1:8) and by ELISA (less than 1:100). However, 14 of 24 (58.3%) of these HSV-1 antibody-negativ...
journal_title:American journal of clinical pathology
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1981-03-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1983-12-01 00:00:00
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pub_type: 杂志文章,多中心研究
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journal_title:American journal of clinical pathology
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更新日期:1995-09-01 00:00:00
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更新日期:1981-03-01 00:00:00
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更新日期:1986-04-01 00:00:00
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更新日期:1998-02-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1983-11-01 00:00:00
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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