Abstract:
:To investigate the specificity of terminal deoxynucleotidyl transferase (TdT) as a marker for lymphoblastic lymphoma in the non-Hodgkin's lymphomas, the authors assayed malignant cells from 142 patients with non-Hodgkin's malignant lymphoma, as well as cells from normal lymphoid tissue controls. Neoplastic cells from all 25 patients with lymphoblastic lymphoma were TdT positive. Of the remaining 117 patients with malignant lymphoma of other histologic subtypes, only one patient, with diffuse, large cell immunoblastic lymphoma, had TdT-positive cells. This patient's cells had positive results only by the enzyme assay and were TdT-negative by both immunofluorescence and immunoperoxidase assays. Of 56 patients whose cells were studied by both enzyme assay and an immunoassay method for TdT detection, a discordant result was obtained only on cells from the previously mentioned patient with large cell immunoblastic lymphoma. To determine the optimal immunoassay method, the authors studied malignant cells from 24 patients with non-Hodgkin's lymphoma for the presence of TdT by three different methods: immunofluorescence, peroxidase-antiperoxidase, and avidin-biotin-complex immunoperoxidase. Fifteen of the 24 patients had lymphoblastic lymphoma. The avidin-biotin-complex method produced superior staining, with no false-negative results. False-negative results were seen with both the immunofluorescence and peroxidase-antiperoxidase methods. Other advantages of the avidin-biotin-complex method included a marked decrease in background and non-specific staining and the ability to use higher dilutions of antisera.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Braziel RM,Keneklis T,Donlon JA,Hsu SM,Cossman J,Bollum FJ,Jaffe ESdoi
10.1093/ajcp/80.5.655subject
Has Abstractpub_date
1983-11-01 00:00:00pages
655-9issue
5eissn
0002-9173issn
1943-7722journal_volume
80pub_type
杂志文章abstract::In the differential diagnosis of Spitz nevus vs malignant melanoma, patient age provides a critical piece of clinical information, because Spitz nevi occur mostly in children and melanomas occur mostly in adults. Nevertheless, there is overlap in the age distributions of Spitz nevus and melanoma. The issue to consider...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/E14C-J6KR-D092-DP3M
更新日期:2004-06-01 00:00:00
abstract::During a 6-month period at Walter Reed Army Hospital the monthly attack rate of Staphylococcus aureus bacteremia increased to 3.8 +/- 0.5 (mean +/- SEM) from 2.5 +/- 0.2 cases per 1,000 dispositions for the previous 48 months (P less than 0.05). A predominant phage pattern, designated S, was found in 12 (39%) of 31 ba...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/79.5.598
更新日期:1983-05-01 00:00:00
abstract::Chicken pox, although common, is rarely associated with autoimmune hemolytic anemia. Reported here is the case of an 8-year-old boy who was found to have cold agglutinin disease and severe anemia several days after he contracted chicken pox. The cold agglutinin appeared to be a polyclonal immunoglobulin M antibody wit...
journal_title:American journal of clinical pathology
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更新日期:1992-01-01 00:00:00
abstract::Two hundred serum samples were tested with two assays for rapid detection of cytomegalovirus (CMV) antibodies. The assays were CMV Scan, a two-step latex particle agglutination assay for CMV IgG and IgM antibodies, and TranSTAT-CMV, a newly available six-step solid-phase enzyme immunoassay for CMV IgG antibodies. Comp...
journal_title:American journal of clinical pathology
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更新日期:1993-05-01 00:00:00
abstract::The present study evaluated the origin of endothelial and epithelial cells, as well as of lymphocytes and macrophages, after lung transplantation. Biopsy specimens from patients who underwent lung and heart-lung transplantation and received organs of sex-mismatched donors were studied by means of nonisotopic in situ h...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/GAFN-5MPA-LY8E-DTPQ
更新日期:2001-04-01 00:00:00
abstract::Possible increased activation of the coagulation pathway was measured in a group of patients with neoplastic diseases. In addition to standard tests, the thromboplastin generation test, thrombin generation test and immunologic and coagulant activities of both Factor VIII and antithrombin III were utilized in the evalu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/68.2.258
更新日期:1977-08-01 00:00:00
abstract::Vitamin E has been reported to increase serum high-density lipoprotein (HDL) cholesterol. Given the known inverse relationship between HDL-cholesterol and risk of developing coronary artery disease, these preliminary results require verification or refutation. In the present study, 39 volunteers were given 600 U of vi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.1.86
更新日期:1982-01-01 00:00:00
abstract::To establish the effect of a quality control failure on the performance of the LCx-GC nucleic-acid amplification assay for Neisseria gonorrhoeae (Abbott Laboratories, Abbott Park, IL) in the field, we conducted a retrospective analysis comparing the clinical and analytic performance of the recalled lots with those not...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/1LD8-LU7Y-FFW7-5WQA
更新日期:2005-06-01 00:00:00
abstract:OBJECTIVES:The incidence of lymphoproliferative disorders (LPDs) is increasing in sub-Saharan Africa (SSA) due to population growth, aging, and human immunodeficiency virus (HIV). Despite significant burden, resources for diagnosis and treatment of LPDs are limited, with little infrastructure to deliver modern patholog...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw118
更新日期:2016-10-01 00:00:00
abstract::Respiratory syncytial virus is detected in cell culture by the presence of cytopathic effect. To detect RSV before cytopathic effect is usually seen, slides were evaluated retrospectively from 482 HEp-2 cell cultures on days 2-4 after inoculation. Indirect immunofluorescent staining detected RSV in 57 of 94 cultures t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.127
更新日期:1991-07-01 00:00:00
abstract::Chemotherapy is a well-established therapeutic approach for several malignancies, but its clinical efficacy is often limited by its related cardiotoxicity, which leads to cardiomyopathy, possibly evolving into heart failure. To detect cardiac damage, the adopted diagnostic approach is the estimation of left ventricula...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
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更新日期:2008-11-01 00:00:00
abstract::The hematologic status of 265 patients with rheumatoid arthritis was assessed. In the group as a whole, a mild depression in the hemoglobin concentration and mean cell volume (MCV) was associated with an increase in the red blood cell distribution width (RDW), erythrocyte sedimentation rate (ESR), and platelet count. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/87.2.196
更新日期:1987-02-01 00:00:00
abstract::Twenty-five cases of tubal or tubo-endometrioid metaplasia that occurred in patients from 21 to 51 years of age (mean 39 years) are described. The majority of the cases were seen in consultation. They were referred because of diagnostic problems resulting from features that have received little emphasis. The metaplasi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.5.618
更新日期:1995-05-01 00:00:00
abstract::The probabilities of low transferrin saturation, folic acid, or vitamin B12 levels in association with various erythrocyte indices was determined from the prevalences of these abnormalities and the distributions of the indices among 206 hospitalized and 1,000 ambulatory anemic patients. At mean corpuscular hemoglobin ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.5.748
更新日期:1978-11-01 00:00:00
abstract::Determination of urinary homovanillic acid (HVA) is an important analysis to establish the diagnosis and prognosis of neuroblastoma. A method is presented for the determination of this metabolite of dopamine using its reaction with 1-nitroso-2-naphthol and nitrous acid. The method has shown a sensitivity, specificity,...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1987-05-01 00:00:00
abstract::A patient with prolymphocytic leukemia was immunophenotyped by whole blood and Ficoll-Hypaque methods. Discordance was noted between the two methods, especially for the HLE1, B1, I2, and T1B monoclonal markers. The disparity in results reflected the use of 15 X 10(6) cells versus 1 X 10(6) cells per reaction tube. Thi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.2.227
更新日期:1989-02-01 00:00:00
abstract::Mantle cell lymphoma (MCL) expresses pan-B-cell antigens and is usually CD5+/CD10-/CD23-/FMC7+. In this study, we evaluated 52 patients with confirmed diagnoses of MCL and identified variant immunophenotypes in 21 patients (19/48 classical and 2/4 variant MCLs), including CD5- in 6 (12%) of 52, CD10+ in 4 (8%) of 50, ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPV8LN5ENMZOVY
更新日期:2009-11-01 00:00:00
abstract::We further characterize the heterogeneous carcinomas of the papilla of Vater (CPVs) in relation to various clinicopathologic patient characteristics and patient survival. Of the 71 reevaluated CPVs, 32 were intestinal, 26 were pancreatobiliary, 6 were mixed, 4 were mucinous, and 3 were poorly differentiated carcinomas...
journal_title:American journal of clinical pathology
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doi:10.1309/AJCPCTCUQSYI89YT
更新日期:2011-02-01 00:00:00
abstract::A 65-year-old man with lower back pain was found to have multiple myeloma. Unique findings in the marrow aspirate smears of this patient were tadpolelike plasma cells and cellular rosettes simulating those seen in neuroblastoma. This case documents yet another facet of the broad morphologic spectrum of neoplastic plas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
abstract::Mucocele-like tumors of the breast originally were reported by Rosen in 1986 as benign lesions that histologically resembled colloid carcinoma of the breast. The authors document two cases of mucocele-like tumors to illustrate the difficulty in separating these lesions from colloid carcinoma on the basis of fine-needl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) and immunohistochemical analysis for assessment of HER-2 status in breast carcinomas are discordant in a significant proportion of cases with equivocal (2+) immunostaining. To evaluate the role of intratumoral heterogeneity and degree of amplification, we performed additional ...
journal_title:American journal of clinical pathology
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更新日期:2005-08-01 00:00:00
abstract::The incubation of stored blood in a mixture of inosine, pyruvate, glucose, and phosphate restores the O2 affinity of hemoglobin to physiologic levels, as measured by the configuration of the dissociation curve and the P50. This regeneration of normal hemoglobin function not only is consistent for samples anticoagulate...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/68.1.53
更新日期:1977-07-01 00:00:00
abstract::It has been suggested that kallikrein inhibition may predispose patients with the lupus inhibitor to thrombosis by interfering with the Factor XII-mediated activation of plasminogen. To further investigate this suggestion, the authors measured kallikrein inhibition in 19 patients with the lupus inhibitor. They found t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.2.223
更新日期:1987-08-01 00:00:00
abstract::We report 2 cases of splenic postchemotherapy histiocyte-rich pseudotumor. Each patient had a history of diffuse large B-cell lymphoma, treated with multiagent chemotherapy. Computed tomography scans performed on both patients showed splenic masses. A positron emission tomography scan performed on 1 patient showed inc...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPC67QOGJXCEZB
更新日期:2009-09-01 00:00:00
abstract::Mucocele-like lesion (MLL) is a rare mucinous lesion of the breast with highly variable upgrade rates to atypia or malignancy on excision. This spectrum of data has led to differing opinions on the need for surgical excision. We evaluated 50 core biopsy specimens diagnosed as having MLLs and correlated the findings wi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP1D8YLCFFTLOW
更新日期:2012-12-01 00:00:00
abstract::Little is known concerning the pathology of spleens removed for traumatic injury. The authors studied the gross and microscopic features of 44 spleens removed for trauma and received at the Surgical Pathology Division of Parkland Memorial Hospital and 10 normal control spleens from the Medical Examiner's Office, Dalla...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/105.4.474
更新日期:1996-04-01 00:00:00
abstract::Forty-five renal biopsies with amyloidosis were studied by light microscopy with Congo red staining and action of potassium permanganate and by immunofluorescence with antihuman tissue A component antiserum antilight and heavy chains of immunoglobulins antisera. The patients were classified on the basis of concordance...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/87.6.756
更新日期:1987-06-01 00:00:00
abstract:OBJECTIVES:The metaplastic intestinal epithelium in Barrett esophagus (BE) occasionally contains Paneth cells; however, little is known regarding the prevalence and significance of Paneth cell metaplasia (PCM) in BE. METHODS:We evaluated 757 esophageal biopsy specimens with intestinal metaplasia (IM) for PCM. Outcome ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPVUJMCVBC9PKM
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVES:Cytogenetics defines disease entities and predicts prognosis in acute myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of the genome, while fluorescence in situ hybridization (FISH) detects targeted abnormalities. The aim of this study was to compare the utility of karyotyping a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPP6LVMQG4LNCK
更新日期:2015-06-01 00:00:00
abstract::We describe 41 cases of myeloid neoplasms (MNs) secondary to plasma cell myeloma (PCM). The types of MN included myelodysplastic syndrome (MDS) in 34 (82.9%), acute myeloid leukemia (AML) in 4 (9.8%), and myeloproliferative neoplasm (MPN) or MDS/MPN in 3 (7.3%) cases. The latency from treatment to diagnosis of MN rang...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1309/AJCPOP7APGDT9JIU
更新日期:2012-12-01 00:00:00