Restoration of hemoglobin function in stored EDTA blood. Application in identification of hemoglobin variants with abnormal oxygen affinity.

Abstract:

:The incubation of stored blood in a mixture of inosine, pyruvate, glucose, and phosphate restores the O2 affinity of hemoglobin to physiologic levels, as measured by the configuration of the dissociation curve and the P50. This regeneration of normal hemoglobin function not only is consistent for samples anticoagulated with EDTA and stored eight days at 4 C but also is demonstrable for at least 19 days of 4 C storage of EDTA-anticoagulated whole blood. This regeneration procedure is simple to perform and makes it possible to measure reliably O2 affinity in blood samples transmitted by mail.

journal_name

Am J Clin Pathol

authors

Sumoza A,Fairbanks VF,Pineda AA

doi

10.1093/ajcp/68.1.53

subject

Has Abstract

pub_date

1977-07-01 00:00:00

pages

53-6

issue

1

eissn

0002-9173

issn

1943-7722

journal_volume

68

pub_type

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