Abstract:
OBJECTIVES:Cytogenetics defines disease entities and predicts prognosis in acute myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of the genome, while fluorescence in situ hybridization (FISH) detects targeted abnormalities. The aim of this study was to compare the utility of karyotyping and FISH in adult AML. METHODS:We studied 250 adult AML cases with concurrent karyotyping and FISH testing. Karyotyping was considered adequate when 20 or more metaphases were analyzed. RESULTS:In total, 220 cases had adequate karyotyping and were classified as normal karyotype/normal FISH (n = 92), normal karyotype/abnormal FISH (n = 4), abnormal karyotype/normal FISH (n = 8), and abnormal karyotype/abnormal FISH (n = 116). The overall karyotype/FISH concordance rate was 97.7% with five discordant cases identified, four from the normal karyotype/abnormal FISH group and one from the abnormal karyotype/abnormal FISH group. No karyotype/FISH discordance was seen in the abnormal karyotype/normal FISH group for the FISH probes evaluated. FISH lent prognostic information in one (0.5%) of 220 cases with normal karyotype/abnormal FISH: CBFB-MYH11 fusion, indicating favorable prognosis. CONCLUSIONS:In adult AML, FISH rarely provides additional information when karyotyping is adequate. We therefore propose an evidence-based, cost-effective algorithmic approach for routine conventional karyotype and FISH testing in adult AML workup.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
He R,Wiktor AE,Hanson CA,Ketterling RP,Kurtin PJ,Van Dyke DL,Litzow MR,Howard MT,Reichard KKdoi
10.1309/AJCPP6LVMQG4LNCKsubject
Has Abstractpub_date
2015-06-01 00:00:00pages
873-8issue
6eissn
0002-9173issn
1943-7722pii
143/6/873journal_volume
143pub_type
杂志文章abstract::Myofibromatosis generally is regarded as a fibrous proliferative disease of neonates and children that, even when multicentric, has an excellent prognosis and a propensity for spontaneous involution. Reported is a case of solitary, locally recurrent myofibromatosis in the ear canal of an adult patient. It was initiall...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/97.6.810
更新日期:1992-06-01 00:00:00
abstract::Utilizing a bacterial-agar overlay technic incorporating the methionine-requiring bacterium Leukonostoc mesenteroides, little or no bacterial growth was seen surrounding the megaloblasts and proerythroblasts of eight patients who had severe untreated pernicious anemia. Similarly, scant bacterial growth was observed in...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/65.4.504
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journal_title:American journal of clinical pathology
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abstract:OBJECTIVES:To evaluate the clinical utility of and demand for the creatine kinase (CK)-MB assay. METHODS:We examined the number of CK-MB tests from 2007 through 2013 while we progressively deemphasized their use. We first removed CK-MB from the acute coronary syndrome (ACS) panel and then from the main menu and observ...
journal_title:American journal of clinical pathology
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abstract::The serum concentrations of alpha-fetoprotein of rats following experimental galactosamine-induced liver-cell necrosis accurately reflect the severity of preceding liver-cell damage as determined by elevation of serum transaminases. There is a very close correlation between the highest SGOT or SGPT serum activity foun...
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pub_type: 杂志文章
doi:10.1309/AJCP7ZC9YQERSKGX
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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更新日期:2011-08-01 00:00:00
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journal_title:American journal of clinical pathology
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doi:10.1309/AJCPH2A4XTXJUKDZ
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journal_title:American journal of clinical pathology
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更新日期:1998-05-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.5.655
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journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
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journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
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journal_title:American journal of clinical pathology
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doi:
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journal_title:American journal of clinical pathology
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更新日期:2012-09-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1991-03-01 00:00:00
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更新日期:2011-05-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2016-09-01 00:00:00
abstract::The lupus anticoagulant was identified in three patients. Laboratory studies gave evidence of inhibitory activity directed against phospholipid and prekallikrein. Inhibition of prekallikrein has not been reported previously. When exposed to kaolin, all three patients' plasmas failed to develop the level of fibrinolyti...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/77.3.275
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/88.1.102
更新日期:1987-07-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPZRRL2DGC2ODA
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