EDTA Treatment of Serum Unmasks Complement-Mediated Prozone Inhibition in Human Leukocyte Antigen Antibody Testing.

Abstract:

OBJECTIVES:Luminex-based single-antigen bead human leukocyte antigen (HLA) antibody testing is widely used to define HLA antibodies for transplant compatibility. False-negative results can occur with complement-mediated prozone inhibition. This study assessed the effect of EDTA on the assay background reactivity and fluctuations in antibody mean fluorescent intensity. METHODS:Serum specimens were retrospectively tested using Luminex-based single-antigen beads with and without EDTA. Treated and untreated serum samples were compared by two measures: changes in background reactivity and changes in HLA antibody strength after EDTA treatment. RESULTS:Ten pretransplant and 48 posttransplant specimens were identified: lung (22), heart (10), kidney (21), heart/lung (two), pancreas (one), small bowel (one), and liver (one). After EDTA treatment, weak antibodies (below 2,000 mean florescent intensity) demonstrated the largest fluctuations. Newly identified HLA antibodies were seen in 16% (8/49) of class I and 26% (15/57) of class II beads. EDTA treatment did not result in false-negative reactions compared with untreated serum. CONCLUSIONS:EDTA serum pretreatment mitigated complement-mediated prozone inhibition and improved accurate HLA antibody detection. The background reactivity and the false-negative rate of the assay appear unchanged.

journal_name

Am J Clin Pathol

authors

Anani WQ,Zeevi A,Lunz JG

doi

10.1093/ajcp/aqw116

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

346-52

issue

3

eissn

0002-9173

issn

1943-7722

pii

aqw116

journal_volume

146

pub_type

杂志文章
  • The reticulocyte. An approach to definition.

    abstract::Experiences of the CAP Survey Program in reticulocyte counts and morphologic identification of reticulocytes from 1971 to 1974 are reviewed. Problems of morphologic identification are reflected in an excessive variance of reticulocyte counts. Statistical sources of variations in counting are identified. The older, ori...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Gilmer PR Jr,Koepke JA

    更新日期:1976-07-01 00:00:00

  • Use of commercially prepared control sera as quality control materials for spectrophotometric bilirubin determinations in amniotic fluid.

    abstract::The visible absorption spectra of dilutions of commercially prepared chemistry control sera closely mimic those of jaundiced amniotic fluids in cases of fetal rhesus isoimmunization. The authors have assessed the use of these materials for the quality control of net absorbance measurements at 450 nm on amniotic fluids...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.4.513

    authors: McDonald OL,Watts MT

    更新日期:1985-10-01 00:00:00

  • Clinically relevant differences in prothrombin time and INR values related to blood sample collection in plastic vs glass tubes.

    abstract::We compared prothrombin times (PTs) and international normalized ratios (INRs) for blood samples drawn into plastic vs glass collection tubes. We collected 60 venous blood samples into 4.5-mL glass and 2 plastic tubes (2.7 and 3.5 mL). An additional 153 samples, including 63 from warfarin-anticoagulated patients, were...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Fiebig EW,Etzell JE,Ng VL

    更新日期:2005-12-01 00:00:00

  • The autopsy. Simple thoughts about the public needs and how to address them.

    abstract::Dialogue regarding the autopsy has been sustained largely by medical students and professionals, and by families of deceased patients. Exclusion of the general public may represent a significant reason for the inexorable decline of autopsy rates and politicoeconomic support. Sensitivity to public concerns regarding th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: McManus BM,Wood SM

    更新日期:1996-10-01 00:00:00

  • Immunophenotyping of non-Hodgkin's lymphomas in paraffin-embedded tissue sections. A comparison with genotypic analysis.

    abstract::Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.4.517

    authors: Elghetany MT,Kurec AS,Schuehler K,Forbes BA,Duggan DB,Davey FR

    更新日期:1991-04-01 00:00:00

  • Assessment of The Bethesda System for Reporting Thyroid Cytopathology.

    abstract:OBJECTIVES:Long-term follow-up is important for determining performance characteristics of thyroid fine-needle aspiration (FNA). METHODS:Histologic or 3 or more years of clinical follow-up was used to calculate performance characteristics of thyroid FNA before and after implementation of The Bethesda System for Report...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqz076

    authors: Sauter JL,Lehrke H,Zhang X,Al Badri OT,Rodriguez-Gutierrez R,Delivanis DA,Singh Ospina N,Donegan D,Hamidi O,Iñiguez-Ariza N,Sharma A,Kittah NEN,Tamhane SU,Hurtado Andrade MD,Kotwal A,Jenkins SM,Spears G,Rivera M,Dean

    更新日期:2019-09-09 00:00:00

  • Morphologic and Immunocytochemical Features of High-Grade Serous Carcinoma of Ovary in Ascitic Fluid Effusion and Fine-Needle Aspiration Cytology.

    abstract:OBJECTIVES:High-grade serous carcinoma (HGSC) is the most common ovarian malignancy. The role of cytopathology in obtaining tissue diagnosis before institution of neoadjuvant chemotherapy (NACT) was evaluated. METHODS:All histopathology-proven HGSC specimens between 2015 and 2018 with prior cytopathologic diagnosis by...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa028

    authors: Bansal A,Srinivasan R,Rohilla M,Sundaram A,Rai B,Rajwanshi A,Suri V,Saha SC,Gupta N,Gupta P,Dey P

    更新日期:2020-06-08 00:00:00

  • Genotypic and immunophenotypic characterization of two human light chain-only B-cell non-Hodgkin's lymphomas.

    abstract::Two atypical human non-Hodgkin's lymphomas (NHLs) that exhibited unusual genotypic and in situ immunophenotypic abnormalities are described. Immunophenotypically, both NHLs lacked surface Ig heavy chains. With the exception of the MB2 B-cell-associated antigen, no B- and T-cell differentiation antigen was detected in ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.4.390

    authors: De Re V,Carbone A,Dolcetti R,Tirelli U,Folegotto C,Boiocchi M

    更新日期:1990-10-01 00:00:00

  • Abdominal wall defect associated with persistent cloaca. The embryologic clues in autopsy.

    abstract::Three stillborn fetuses are reported in which an abdominal wall defect was associated with defects in the urogenital and anal region. Autopsy of these fetuses provided clues indicative of how and where the embryonic development went wrong. The malformation involved a disturbance of the cell deposition process, occurri...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.5.640

    authors: Hartwig NG,Steffelaar JW,Van de Kaa C,Schueler JA,Vermeij-Keers C

    更新日期:1991-11-01 00:00:00

  • Presence of t(8;21)(q22;q22) in myeloperoxidase-positive, myeloid surface antigen-negative acute myeloid leukemia.

    abstract::Although acute myeloid leukemias (AMLs) cytochemically negative for myeloperoxidase are now well recognized, myeloid surface antigen-negative AMLs are rare. The morphologic, cytochemical, immunologic, and cytogenetic or molecular features of such cases are described in four adults aged 19 to 60 years. All had AML with...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/107.1.68

    authors: Arber DA,Glackin C,Lowe G,Medeiros LJ,Slovak ML

    更新日期:1997-01-01 00:00:00

  • Assay of Factor XII clotting activity in heparinized plasma.

    abstract::Clotting tests based upon the activated partial thromboplastin time are disturbed by heparin. This brief communication shows that Factor XII clotting activity in plasma samples heparinized in vitro and in vivo can be measured accurately by means of an aPTT-test using congenitally deficient substrate plasma when the pl...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.4.474

    authors: Lämmle B,Tran TH,Duckert F

    更新日期:1983-10-01 00:00:00

  • Patient age in Spitz nevus and malignant melanoma: implication of Bayes rule for differential diagnosis.

    abstract::In the differential diagnosis of Spitz nevus vs malignant melanoma, patient age provides a critical piece of clinical information, because Spitz nevi occur mostly in children and melanomas occur mostly in adults. Nevertheless, there is overlap in the age distributions of Spitz nevus and melanoma. The issue to consider...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/E14C-J6KR-D092-DP3M

    authors: Vollmer RT

    更新日期:2004-06-01 00:00:00

  • Diversity of organ site involvement among malignant lymphomas of mucosa-associated tissues.

    abstract::Fifteen cases of low-grade B-cell lymphoma involving unusual extranodal sites have been studied in comparison to cases reported or observed arising in typical mucosa-associated lymphoid tissues. In every case, histopathologic features conformed to those characteristic for lymphomas of mucosa-associated lymphoid tissue...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.6.738

    authors: Pelstring RJ,Essell JH,Kurtin PJ,Cohen AR,Banks PM

    更新日期:1991-12-01 00:00:00

  • Improved laboratory confirmation of heparin-induced thrombocytopenia type II. Time course of antibodies and combination of antigen and biologic assays.

    abstract::We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Harenberg J,Wang L,Hoffmann U,Huhle G,Feuring M

    更新日期:2001-03-01 00:00:00

  • The effect of the platelet count on the aggregation response and adenosine triphosphate release in an impedance lumi-aggregometer.

    abstract::Platelet aggregation studies are usually performed in either electrical impedance or optical systems and the release reaction assessed by quantitating adenosine triphosphate (ATP) from luminesence produced by the firefly luciferin-luciferase system. In evaluating the results of such studies, attention is paid to a var...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.5.655

    authors: Sweeney JD,Labuzetta JW,Fitzpatrick JE

    更新日期:1988-05-01 00:00:00

  • A hospital-wide outbreak of septicemia due to a few strains of Staphylococcus aureus.

    abstract::During a 6-month period at Walter Reed Army Hospital the monthly attack rate of Staphylococcus aureus bacteremia increased to 3.8 +/- 0.5 (mean +/- SEM) from 2.5 +/- 0.2 cases per 1,000 dispositions for the previous 48 months (P less than 0.05). A predominant phage pattern, designated S, was found in 12 (39%) of 31 ba...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.5.598

    authors: Cross AS,Zierdt CH,Roup B,Almazan R,Swan JC

    更新日期:1983-05-01 00:00:00

  • Reliability and significance of increased creatine kinase MB isoenzyme in the serum of uremic patients.

    abstract::The authors quantified creatine kinase MB (CK-MB) isoenzyme activity and mass in the serum of 81 uremic and 20 nonuremic (control) patients who had no clinical or electrocardiographic evidence of acute myocardial infarction. CK-MB was quantified by three methods: electrophoresis, the QuiCK-MB (International Immunoassa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/87.1.103

    authors: Medeiros LJ,Schotte D,Gerson B

    更新日期:1987-01-01 00:00:00

  • Lymphoepithelioma-Like Carcinoma of the Uterine Cervix: A Pathologic Study of Eight Cases With Emphasis on the Association With Human Papillomavirus.

    abstract:Objectives:Lymphoepithelioma-like carcinoma (LELC) of the uterine cervix is a rare tumor. The goal of this study was to evaluate a series of cases of cervical LELC and to investigate possible association with human papillomavirus (HPV) and/or Epstein-Barr virus (EBV). Methods:Immunohistochemistry for p63, p16, human l...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqy130

    authors: Pinto A,Huang M,Nadji M

    更新日期:2019-01-07 00:00:00

  • Can We Identify Nephrogenic Adenoma in Urine Cytology Specimens? A Study Evaluating Previously Described Cytomorphologic Features in Correlation With PAX8 Immunohistochemical Staining Results.

    abstract:OBJECTIVES:The aim of this study is to determine if the diagnosis of nephrogenic adenoma (NA) can be made on cytologic criteria alone and if pair box gene transcription factor 8 (PAX8) is useful in the diagnosis of NA in daily cytology practice. METHODS:Cytologic features of NA previously described in a literature wer...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqw006

    authors: McCroskey Z,Pambuccian SE,Wojcik EM,Barkan GA

    更新日期:2016-03-01 00:00:00

  • Automated immunohistochemical estrogen receptor in fixed embedded breast carcinomas. Comparison with manual immunohistochemistry on frozen tissues.

    abstract::Estrogen receptor (ER) status of breast carcinomas determines prognosis and treatment. Biochemical ER assays are expensive and time-consuming and require fresh tumor. Immunohistochemical ER was assessed in 68 breast carcinomas, by an automated method using routinely processed formalin-fixed paraffin-embedded tissues, ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/92.5.669

    authors: Cohen C,Unger ER,Sgoutas D,Bradley N,Chenggis M

    更新日期:1989-11-01 00:00:00

  • Lymphocytic mastopathy. An autoimmune disease of the breast?

    abstract::Eight cases are reported of an unusual breast lesion consisting of dense intralobular, perilobular, and perivascular lymphocytic infiltrates associated with lobular atrophy and sclerosis. Immunologic studies on paraffin-embedded tissue (seven cases) and frozen tissue (two cases) demonstrated a predominance of B-cells ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/93.6.725

    authors: Schwartz IS,Strauchen JA

    更新日期:1990-06-01 00:00:00

  • Distribution and incidence of viridans streptococcal species in routine clinical specimens.

    abstract::Five hundred consecutive isolates of viridans streptococci were identified to the species level in an effort to determine their distribution and incidence in routine clinical specimens. Viridans streptococci accounted for significant percentages of streptococcal isolates from urine, wounds, body fluids, and blood. The...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.6.854

    authors: Ruoff KL,Fishman JA,Calderwood SB,Kunz LJ

    更新日期:1983-12-01 00:00:00

  • Immunohistochemistry of Warthin's tumor.

    abstract::This study describes results of fresh-frozen immunohistochemistry applied to two typical Warthin's tumors. Polyclonal follicular center cell staining and an apparent reduction in the number of T-lymphocytes were found. These observations tend to support the concept that the lymphoid infiltrate in Warthin's tumor repre...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.6.795

    authors: Tubbs RR,Sheibani K,Weiss RA,Lee V,Sebek BA,Valenzuela R

    更新日期:1980-12-01 00:00:00

  • The diagnostic role of claudins in serous effusions.

    abstract::We analyzed the diagnostic role of claudins in effusion cytology in 325 effusions, including 218 ovarian, 49 breast, 15 cervical or endometrial, 10 gastrointestinal, and 8 lung adenocarcinomas and 25 malignant mesotheliomas (MMs). Specimens were analyzed for claudin-1 and claudin-3 expression using immunohistochemical...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/V025QRN3R9CJGNPX

    authors: Kleinberg L,Holth A,Fridman E,Schwartz I,Shih IeM,Davidson B

    更新日期:2007-06-01 00:00:00

  • Detection of chromosome 11q13 breakpoints by interphase fluorescence in situ hybridization. A useful ancillary method for the diagnosis of mantle cell lymphoma.

    abstract::We assessed cytologic specimens from 11 mantle cell lymphomas (MCLs) and 32 other B-cell non-Hodgkin lymphomas (NHLs) for 11q13 breakpoints using a 2-color fluorescence in situ hybridization (FISH) assay that uses an 11q13 probe centered on the CCND1 gene and a centromeric chromosome 11 probe (CEP11). The number of nu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/69EJ-RFM5-E976-BUTP

    authors: Katz RL,Caraway NP,Gu J,Jiang F,Pasco-Miller LA,Glassman AB,Luthra R,Hayes KJ,Romaguera JE,Cabanillas FF,Medeiros LJ

    更新日期:2000-08-01 00:00:00

  • Prospects of cure in lymphocyte-predominant Hodgkin's disease.

    abstract::This report concerns a particular benign histologic variant of Hodgkin's disease, the lymphocyte-predominant category of the Rye classification. The group of 24 cases, all involving localized disease, was first reported in 1960, and is now reappraised, incorporating subsequent data. The interpretations of the original...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/67.6.507

    authors: Wright CJ

    更新日期:1977-06-01 00:00:00

  • Myelodysplasia in the acquired immune deficiency syndrome.

    abstract::Certain new hematologic findings in eight homosexual or bisexual patients with the acquired immune deficiency syndrome (AIDS) are presented. All eight patients manifested a normochromic, normocytic anemia, and six of eight had granulocytopenia during their hospitalization. The other two had low-normal granulocyte coun...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.2.144

    authors: Schneider DR,Picker LJ

    更新日期:1985-08-01 00:00:00

  • Ultrastructure of the gastric mucosa harboring Campylobacter-like organisms.

    abstract::The association between Campylobacter-like organisms (CLOs) and lesions of the gastric mucosa was studied in 59 consecutive biopsies. Hematoxylin and eosin and Warthin-Starry silver stains, as well as high-resolution light microscopy (HRLM) and transmission electron microscopy (TEM), were used. The organisms were foun...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.5.575

    authors: Chen XG,Correa P,Offerhaus J,Rodriguez E,Janney F,Hoffmann E,Fox J,Hunter F,Diavolitsis S

    更新日期:1986-11-01 00:00:00

  • Primary carcinoid tumor of the liver.

    abstract::A primary hepatic tumor with histologic features suggestive of carcinoid tumor is reported, and six previously reported cases reviewed. Tumor cells contained electron dense secretory granules with an average diameter 150-200 nm which demonstrated positive argyrophilic reactions. On immunohistochemistry, chromogranin A...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.4.561

    authors: Miura K,Shirasawa H

    更新日期:1988-04-01 00:00:00

  • Direct comparison of two mechanized systems for identification of gram-negative bacilli. Autobac ID system versus the auto microbic system (with EBC plus).

    abstract::The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.4.462

    authors: Barry AL,Gavan TL,Badal RE,Telenson MJ

    更新日期:1982-10-01 00:00:00