Abstract：
:Estrogen receptor (ER) status of breast carcinomas determines prognosis and treatment. Biochemical ER assays are expensive and time-consuming and require fresh tumor. Immunohistochemical ER was assessed in 68 breast carcinomas, by an automated method using routinely processed formalin-fixed paraffin-embedded tissues, and manually with the use of snap-frozen tissues with a monoclonal anti-ER and peroxidase-antiperoxidase technique. The paraffin sections were digested with DNase to enhance development of signal. Positive nuclear ER was obtained in 9 (13%) fixed tissues and 36 (53%) frozen tissues. The sensitivity, specificity, and predictive value of a positive test result, as compared with the biochemical assay, were 25%, 100%, and 100% for the paraffin section technique, and 89%, 88%, and 89% for the frozen sections. Although it is specific, lack of sensitivity, resulting from loss of ER with fixation and room temperature handling, renders this immunohistochemical technique unacceptable on fixed tissues. However, ER immunostain on frozen tissue is an acceptable alternative to biochemical assay.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Cohen C,Unger ER,Sgoutas D,Bradley N,Chenggis Mdoi
10.1093/ajcp/92.5.669subject
Has Abstractpub_date
1989-11-01 00:00:00pages
669-72issue
5eissn
0002-9173issn
1943-7722journal_volume
92pub_type
杂志文章abstract：:The characteristics of the normal adult thymus in both sexes were determined in 50 cases of accidental death by a simplified quantitative histologic technic. A table of normal values derived from these findings was used for making comparisons with thymus from autopsies of 50 additional patients suffering terminal illn...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.5.657
更新日期：1981-11-01 00:00:00
abstract：OBJECTIVES:In 2001, the University of New Mexico Hospitals implemented a rapid screening tool for the triage of suspected hantavirus cardiopulmonary syndrome based on peripheral blood smear morphology. Five criteria guided clinical decisions: thrombocytopenia, hemoconcentration, granulocytic left shift, absence of toxi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPNFVWG46NUHED
更新日期：2014-08-01 00:00:00
abstract：:A 65-year-old man with lower back pain was found to have multiple myeloma. Unique findings in the marrow aspirate smears of this patient were tadpolelike plasma cells and cellular rosettes simulating those seen in neuroblastoma. This case documents yet another facet of the broad morphologic spectrum of neoplastic plas...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/98.6.630
更新日期：1992-12-01 00:00:00
abstract：:Cytogenetic studies of bone marrow were performed in 230 consecutive cases of patients with chronic granulocytic leukemia (CGL) admitted to the National Cancer Institute since 1961. Twenty patients lacked the Philadelphia (Ph1) chromosome. All were previously untreated. When compared with Ph1-positive patients, CGL pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/65.4.467
更新日期：1976-04-01 00:00:00
abstract：:Two monoclonal antibodies, UCD/AB 6.11 and UCD/PR 10.11, were evaluated for their patterns of immunohistochemical reactivity in a survey of paraffin-embedded human tissues by the avidin-biotin-immunoperoxidase technique. By two-dimensional immunoblotting, UCD/AB 6.11 reacts with keratin number 18 and UCD/PR 10.11 iden...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.4.472
更新日期：1988-04-01 00:00:00
abstract：OBJECTIVES:Peritoneal mesothelial cysts have been reported under various terms, including benign cystic mesothelioma, usually in the form of case reports/series, whereas extraperitoneal cases are rarely reported. Our objective was to report the detailed characteristics of cystic lesions of the serosal cavities. METHOD...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa189
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:The objective of this research was to determine test intervals between intraoperator case reviews to minimize the impact of recall. METHODS:Three pathologists were presented with a group of 120 slides and subsequently challenged with a study set of 120 slides after 2-week and 4-week intervals. The challenge...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPUC3TYMS3QOBM
更新日期：2015-03-01 00:00:00
abstract：:Mechanical release of single cell suspensions from solid tumors for DNA analysis by flow cytometry has been shown to be optimal for adenocarcinomas in general. In the breast, many adenocarcinomas are fibrotic or scirrhous, creating difficulty in separating the neoplastic cells from the stroma. The authors have conduct...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.1.8
更新日期：1995-01-01 00:00:00
abstract：:Little information has been reported describing antigen stability in plasma cell myeloma. In this study, the expression frequency and stability of 2 potential therapeutic targets, CD20 and CD52, along with the frequently aberrantly expressed CD56 antigen, were evaluated by flow cytometric analyses in 56 patients with ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/8UVF7YQ1D4D4ETQV
更新日期：2008-06-01 00:00:00
abstract：:CD163, a hemoglobin scavenger receptor, is expressed in monocytes and macrophages. Recent work has shown that this marker is specific for neoplasms of histiocytic differentiation. Our aim was to test the ability of CD163 to separate cutaneous histiocytomas from their morphologic mimics. We tested the expression of CD1...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP88IRBPXQZYIR
更新日期：2010-06-01 00:00:00
abstract：:Riedel thyroiditis (RT) is an uncommon form of chronic thyroiditis in which the thyroid gland is replaced by fibrous tissue. The etiologic mechanisms underlying RT are unclear: the prevailing view is that it is part of a generalized fibroinflammatory process also involving other organs. The clinical manifestations of ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/NUU88VAFR9YEHKNA
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVES:We describe the morphologic and immunohistochemical features of 17 endometrial stromal neoplasms, 16 sarcomas, and one stromal nodule. METHODS:We reviewed 35 cases interpreted as endometrial stromal neoplasms, but 17 high-grade endometrial stromal sarcomas (ESS) and one case of mixed endometrial sarcoma and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPS88CMJRXZBWA
更新日期：2014-06-01 00:00:00
abstract：:A survey of adult patients admitted to a general hospital showed that 1.5% had hepatitis B surface antigen in the blood and 22% had hepatitis antibody. The majority of patients with hepatitis B did not have clinical hepatitis and would not have been recognized without screening all hospital admissions for hepatitis B ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/67.3.257
更新日期：1977-03-01 00:00:00
abstract：:Tissue specimens from a series of 46 hepatic tumors occurring in young female oral contraceptive users were tested for alpha1-antitrypsin deposition, utilizing immunocytochemical and histochemical methods. In two instances serum alpha1-antitrypsin phenotyping was also performed. Immunoreactive alpha1-antitrypsin depos...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.6.736
更新日期：1977-12-01 00:00:00
abstract：:Primary gastric non-Hodgkin lymphoma of mucosa-associated lymphoid tissue (MALT-NHL) is strongly related to Helicobacter pylori (H pylori) infection. CagA positive H pylori strains appear to be more aggressive, as reflected by a greater degree of gastric inflammation and higher levels of Il-8 production. Using a polym...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/106.5.670
更新日期：1996-11-01 00:00:00
abstract：:Using digital cell image analysis performed on Feulgen-stained nuclei, the nuclear characteristics of prostatic neoplasia, ranging from benign (benign prostatic hyperplasia [BPH]), through dysplastic (prostatic intraepithelial neoplasia [PIN] 1-3), to carcinoma were studied. Four histopathologic groups were studied: g...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.5.628
更新日期：1991-11-01 00:00:00
abstract：:Proliferative lesions, which include collagen, elastic fibers and abnormally oriented muscle fibers, were found to develop in intramyocardial arteries more rapidly with age in cigarette smokers than in nonsmokers. The study attempted to determine whether these lesions reduce perfusion pressures in the subendocardial z...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.4.493
更新日期：1977-10-01 00:00:00
abstract：:We obtained data on laboratory turnaround time (TAT) and emergency department (ED) length of stay (LOS). We correlated potassium test TAT outlier percentage (TAT-OP) with ED LOS and found that for each outlier percentage (potassium result > 40 minutes), a projected impact on ED LOS was approximately 2.8 additional min...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/3J5P-9VJR-UP4U-5RU5
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to identify laboratory parameters representing erythrocyte engraftment to be used as an indicator to change the recipient to donor ABO group and Rh type following an ABO-incompatible hematopoietic stem cell transplant (HSCT). Studies have shown that ABO incompatibility does not ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa006
更新日期：2020-05-05 00:00:00
abstract：:The prehospital environment has not been studied for point-of-care testing. Therefore, the authors' helicopter program evaluated the performance of the i-STAT Portable Clinical Analyzer, a rapid point-of-care, hand-held instrument. The primary aim of the study was to determine if the i-STAT Portable Clinical Analyzer ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Clq precipitins were assayed in a model system by absorption nephelometry. Polyethylene glycol was used to extract immune complexes so that sufficient sensitivity with minimal interference could be achieved. Using a double beam spectrophotometer, we found that the reaction of immune complexes with Clq occurred rapidly...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.4.451
更新日期：1983-04-01 00:00:00
abstract：:Apocrine carcinoma (AC) is an uncommon, poorly characterized type of breast tumor. In this review, 55 patients with intraductal (ID) AC and 17 patients with infiltrating (IF) AC were analyzed retrospectively to define the histologic features and clinical course of this neoplasm. Recurrences in the breast occurred in 3...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.4.371
更新日期：1990-10-01 00:00:00
abstract：:This article describes the seventh known case of blue nevus of the lymph node capsule and reviews the previously reported cases. The migration arrest hypothesis as an explanation for the occurrence of this lesion is favored rather than the "benign metastasis" hypothesis. The importance of the recognition of this pheno...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.3.367
更新日期：1984-03-01 00:00:00
abstract：Objectives:Diagnosis of B-cell acute lymphoblastic leukemia (B-ALL) requires immunophenotypic evidence of B-lineage and absence of specific myeloid or T-lineage markers. Rare cases of otherwise typical B-ALL express myeloperoxidase (MPO) detectable by flow cytometry with an absence of other myeloid markers, but the cli...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqx021
更新日期：2017-04-01 00:00:00
abstract：Objectives:To evaluate the prevalence of potentially unnecessary repeat testing (PURT) and the associated economic burden for an inpatient population at a large academic medical facility. Methods:We evaluated all inpatient test orders during 2016 for PURT by comparing the intertest times to published recommendations. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqy020
更新日期：2018-05-31 00:00:00
abstract：:The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polymarker (LDLR, GYPA, ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/KAWA-QHBU-6D1J-XY3G
更新日期：2002-09-01 00:00:00
abstract：:Fatty acid synthase is an enzyme that catalyzes the synthesis of long-chain fatty acids. The enzyme expression is minimal in adult tissues and very high in many cancers. Ulcerative colitis is a chronic inflammatory bowel disease that, when long-standing, is associated with an increased risk of colon cancer. The aim of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/pubvqndnvqkjvc8m
更新日期：2006-07-01 00:00:00
abstract：:Poor core laboratory performance that causes delays in diagnosis and treatment is an impediment to optimal patient care, particularly in high-volume patient care areas such as the emergency department (ED). To evaluate the impact of laboratory performance on patient care outcomes, we obtained data from 11 hospitals re...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/E9QP-VQ6G-2FBV-MJ3B
更新日期：2005-11-01 00:00:00
abstract：:A patient with prolymphocytic leukemia was immunophenotyped by whole blood and Ficoll-Hypaque methods. Discordance was noted between the two methods, especially for the HLE1, B1, I2, and T1B monoclonal markers. The disparity in results reflected the use of 15 X 10(6) cells versus 1 X 10(6) cells per reaction tube. Thi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.2.227
更新日期：1989-02-01 00:00:00
abstract：:Fresh open lung biopsy material from 57 patients was incubated with fluoresceinated complement and immunoglobulin antisera. An indirect immunofluorescent assay using neonatal lung as substrate was conducted as well. Direct immunofluorescent patterns could be categorized into interalveolar septal capillary deposition, ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/EHUQ-HW6K-0G41-E0DX
更新日期：2003-02-01 00:00:00
