Abstract:
:Two monoclonal antibodies, UCD/AB 6.11 and UCD/PR 10.11, were evaluated for their patterns of immunohistochemical reactivity in a survey of paraffin-embedded human tissues by the avidin-biotin-immunoperoxidase technique. By two-dimensional immunoblotting, UCD/AB 6.11 reacts with keratin number 18 and UCD/PR 10.11 identifies both keratin numbers 8 and 18, the major keratins found in human simple epithelial cells. Both antibodies have excellent signal-to-noise ratios, and specifically react with simple epithelia, transitional epithelia, mesothelia and tumors derived from such tissues, making them superior immunological reagents. They do not react with neural, muscle, hematopoietic, connective or most epidermal tissues. However, within a given tissue or cell type, differences in the distributions of the antigens recognized by these two antibodies can be observed, raising the possibility of differential expression, modification, or masking of the keratin epitopes revealed by UCD/AB 6.11 and UCD/PR 10.11.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Chan R,Edwards BF,Hu R,Rossitto PV,Min BH,Lund JK,Cardiff RDdoi
10.1093/ajcp/89.4.472subject
Has Abstractpub_date
1988-04-01 00:00:00pages
472-80issue
4eissn
0002-9173issn
1943-7722journal_volume
89pub_type
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journal_title:American journal of clinical pathology
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更新日期:2005-06-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1986-11-01 00:00:00
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更新日期:2001-11-01 00:00:00
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更新日期:1992-04-01 00:00:00
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更新日期:1988-09-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 临床试验,杂志文章
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更新日期:1986-05-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1993-05-01 00:00:00
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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更新日期:1989-10-01 00:00:00
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更新日期:1993-01-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1978-09-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2006-08-01 00:00:00