Surfactant protein A expression in human normal and neoplastic breast epithelium.

Abstract:

:We studied the presence of surfactant protein A (Sp-A) immunoreactivity and messenger RNA in 62 normal and abnormal breast samples. Sections were immunostained with polyclonal anti-Sp-A antibody. The association between Sp-A immunoreactivity and histologic grade of 32 invasive ductal carcinomas was assessed by 3 pathologists who scored the intensity of Sp-A immunoreactivity times the percentage of tumor immunostained; individual scores were averaged, and the final scores were correlated with tumor grade, proliferative index, and expression of estrogen and progesterone receptors. Strong Sp-A immunoreactivity was present at the luminal surface of ductal epithelial cells in normal breast samples and in benign lesions; carcinomas displayed variable immunoreactivity, inversely proportional to the degree of differentiation. Sp-A messenger RNA was detected by reverse transcriptase-polymerase chain reaction in 3 of 3 normal breast samples and 9 of 9 carcinomas. The significance of Sp-A expression in breast epithelium requires further study; possibly it has a role in native host defense or epithelial differentiation.

journal_name

Am J Clin Pathol

authors

Braidotti P,Cigala C,Graziani D,Del Curto B,Dessy E,Coggi G,Bosari S,Pietra GG

doi

10.1309/DU7F-XYP8-1DF6-WM9E

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

721-8

issue

5

eissn

0002-9173

issn

1943-7722

journal_volume

116

pub_type

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