Abstract：
OBJECTIVES:In 2001, the University of New Mexico Hospitals implemented a rapid screening tool for the triage of suspected hantavirus cardiopulmonary syndrome based on peripheral blood smear morphology. Five criteria guided clinical decisions: thrombocytopenia, hemoconcentration, granulocytic left shift, absence of toxic changes, and more than 10% immunoblasts. Smears meeting four of five criteria were previously shown to have high predictive value for infection. Our retrospective study aimed to determine clinical performance of this test over the past decade. METHODS:Computerized records of 188 smear results were compared with serology. RESULTS:Receiver operator characteristic curve analysis confirmed that the four of five cutoff was the most clinically useful, with sensitivity and specificity of 89% and 93%, respectively. All patients meeting five of five criteria had confirmed infections. Fifteen discordant results were uncovered, explained by positive subsequent tests in the same patient or severe disease without further testing. CONCLUSIONS:Our findings confirm that peripheral smear analysis is clinically useful in this endemic region.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Dvorscak L,Czuchlewski DRdoi
10.1309/AJCPNFVWG46NUHEDsubject
Has Abstractpub_date
2014-08-01 00:00:00pages
196-201issue
2eissn
0002-9173issn
1943-7722pii
142/2/196journal_volume
142pub_type
杂志文章abstract：:The standard method for detecting antinuclear antibodies (ANAs) is by immunofluorescence assay (IFA), a method that is labor intensive and subjective. In an attempt to overcome these limitations, several commercial enzyme immunoassays (EIAs) have been developed. We report the results of our evaluation of the ANA Micro...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/111.4.503
更新日期：1999-04-01 00:00:00
abstract：:A monoclonal antibody prepared against estrogen receptor has been shown to be specific and sensitive for the detection of estrogen receptor in human breast lesions by use of immunohistochemical methods. Two hundred selected cases of primary breast carcinoma were assayed for estrogen receptor content by biochemical and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.3.233
更新日期：1988-09-01 00:00:00
abstract：:B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, includi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/29DA-CY9K-BHNW-4BG6
更新日期：2005-08-01 00:00:00
abstract：OBJECTIVES:Special AT-rich binding protein 2 (SATB2) immunohistochemistry (IHC) has high sensitivity and specificity for colorectal adenocarcinoma (CRC), but data on its expression in specific subsets of pulmonary, gastric, small bowel, and pancreatobiliary adenocarcinomas (ADCAs) are relatively limited or discordant. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa118
更新日期：2021-01-04 00:00:00
abstract：:Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/97.1.108
更新日期：1992-01-01 00:00:00
abstract：:Anti-glomerular basement membrane (anti-GBM) glomerulonephritis is a rare disease caused by IgG autoantibodies against the glomerular basement membrane. We describe clinical and pathologic findings in a series of renal biopsy specimens from 80 patients. The patients ranged in age from 16 to 87 years. The age distribut...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/nptp-4ukv-7ju3-elmq
更新日期：2006-03-01 00:00:00
abstract：OBJECTIVES:Nucleic acid amplification tests on formalin-fixed, paraffin-embedded (FFPE) tissue specimens enable Mycobacterium tuberculosis complex (MTB) detection and rapid tuberculosis diagnosis in the absence of microbiologic culture tests. We aimed to evaluate the efficacy of different polymerase chain reaction (PCR...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP2QZRH4ZNPRDD
更新日期：2014-09-01 00:00:00
abstract：:Factor V Leiden (FVR506Q) is a genetic defect in the factor V (FV) molecule that confers resistance to proteolysis by activated protein C (APC) and is the most common abnormality detected in patients studied for hereditary thrombophilia. The initial screening test for this abnormality was a comparison of the activated...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/109.4.387
更新日期：1998-04-01 00:00:00
abstract：:The thickness of eosinophilic band in collagenous colitis (CC) was assessed by 3 methods: histologic estimates (22 observers), conventional measurements using a calibrated micrometric scale (1 observer), and semiautomatic micrometric measurements (1 observer). By the histologic estimate technique, 7.4% of the results ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/K7MH8UCK3MR7Q77L
更新日期：2008-09-01 00:00:00
abstract：:High-volume (200 ml) platelet concentrates prepared by discontinuous-flow centrifugation, with counts as high as ca. 2.0 x 10(12)/l, were stored in either 600 ml-capacity polyvinylchloride bags or 800-ml capacity polyethylene bags. Both polyvinylchloride and polyethylene bags appeared adequate for 24-hour storage of p...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.3.397
更新日期：1980-03-01 00:00:00
abstract：:To verify their clinical usefulness in diagnosis and early response to therapy of sideropenic anemia, we compared the behavior of the reticulocyte parameter (RET-Y), a raw measure dependent on size and content of the cell, generated by the Sysmex XE 2100, with the mean reticulocyte volume (MCVr) and mean reticulocyte ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/W652-95DT-UWK7-U1HH
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVES:Luminex-based single-antigen bead human leukocyte antigen (HLA) antibody testing is widely used to define HLA antibodies for transplant compatibility. False-negative results can occur with complement-mediated prozone inhibition. This study assessed the effect of EDTA on the assay background reactivity and fl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw116
更新日期：2016-09-01 00:00:00
abstract：:Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/74.4.371
更新日期：1980-10-01 00:00:00
abstract：:Tumor cells in the cerebrospinal fluid of an American child with Burkitt's lymphoma are described. The morphology of the cells was demonstrated better by use of the "millipore" technic and hematoxylin and eosin staining than by the Wright-stained smear. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.2.238
更新日期：1975-08-01 00:00:00
abstract：:Determination of hemoglobin A1c (HbA1c) is one of the most important monitoring procedures for long-term control of diabetes mellitus. Several analytical methods have been developed for the measurement of glycohemoglobin (GHb). Those most frequently used are ion-exchange chromatography for HbA1c and affinity chromatog...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.1.89
更新日期：1995-07-01 00:00:00
abstract：:A simple method designed to allow coagulation studies in heparinized blood was evaluated. Citrated plasma containing heparin was incubated with an anion-exchange resin commercially available in tablet form (Heparsorb). After centrifugation of the mixture, coagulation studies were carried out on the supernatant plasma....
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.1.60
更新日期：1981-01-01 00:00:00
abstract：:Two hundred seven mucinous breast carcinomas were morphologically classified into two different groups: pure mucinous carcinomas consisting only of areas with small epithelial islands of solid tumor floating in abundant extracellular mucin, and mixed carcinomas where the tumor contains large areas of mucin, as well as...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/87.2.155
更新日期：1987-02-01 00:00:00
abstract：:Mycobacterium leprae causes leprosy. M leprae strains collected worldwide have been genetically clonal, which poorly explains the varying severity and clinical features of the disease. We discovered a new Mycobacterium species from 2 patients who died of diffuse lepromatous leprosy (DLL). The Mycobacterium was purifie...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPP72FJZZRRVMM
更新日期：2008-12-01 00:00:00
abstract：:Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/6H9844AMMET82DBJ
更新日期：2008-07-01 00:00:00
abstract：:We report serum enzyme patterns in three patients with polymyositis or dermatomyositis whose cases posed potential problems in distinguishing malignancy, hepatocellular damage, and myocardial infarction from myositis. The alanine aminotransferase showed five- to 16-fold elevations. The creatine kinase MB isoenzyme and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.4.556
更新日期：1980-04-01 00:00:00
abstract：:Two commercial enzyme-linked immunosorbent assays (ELISA) for detection of herpes simplex virus (HSV) antigen in direct clinical specimens were compared with cell culture in primary rabbit kidney and MRC-5. With a total of 238 specimens, the sensitivity and specificity of the Dako ELISA method were determined to be 70...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.2.229
更新日期：1986-02-01 00:00:00
abstract：:Laboratory Branch Complement Fixation and FIAX (fluorescence immunoassay) were compared for the detection of antibodies to herpes simplex virus and cytomegalovirus in human sera. Of 68 sera with no antibodies to herpes simplex virus by complement fixation, one specimen was positive by FIAX (specificity, 98.6%). Of 75 ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.3.387
更新日期：1981-03-01 00:00:00
abstract：OBJECTIVES:To evaluate the clinical utility of and demand for the creatine kinase (CK)-MB assay. METHODS:We examined the number of CK-MB tests from 2007 through 2013 while we progressively deemphasized their use. We first removed CK-MB from the acute coronary syndrome (ACS) panel and then from the main menu and observ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPBIK3G4BWEJKO
更新日期：2014-03-01 00:00:00
abstract：:A prospective double-blind trial was performed to investigate the safety of long-term oral anticoagulant control when dosaged adjustment depends on the prothrombin times assessed with one type of thromboplastin being calculated as if assayed by another type. The three main types of thromboplastin, i.e., bovine (Thromb...
journal_title：American journal of clinical pathology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/ajcp/75.3.297
更新日期：1981-03-01 00:00:00
abstract：:Clq precipitins were assayed in a model system by absorption nephelometry. Polyethylene glycol was used to extract immune complexes so that sufficient sensitivity with minimal interference could be achieved. Using a double beam spectrophotometer, we found that the reaction of immune complexes with Clq occurred rapidly...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.4.451
更新日期：1983-04-01 00:00:00
abstract：:Several authors have suggested that a plausible mechanism for variation in reported mean corpuscular volume (MCV) and hematocrit values for Hematology Survey Specimens may be due to the presence of hyperglycemia in the suspending matrix of the specimens, resulting in cell swelling due to an osmotic matrix effect. The ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:The major histocompatibility complex probably plays a crucial role in the efficacy of the cellular immune response against virally infected cervical diseases. Therefore, the allele-specific histocompatibility-related leukocyte antigens (HLA) class I and II expression on normal (n = 10), premalignant (n = 25), and mali...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.1.5
更新日期：1994-01-01 00:00:00
abstract：:To establish the effect of a quality control failure on the performance of the LCx-GC nucleic-acid amplification assay for Neisseria gonorrhoeae (Abbott Laboratories, Abbott Park, IL) in the field, we conducted a retrospective analysis comparing the clinical and analytic performance of the recalled lots with those not...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/1LD8-LU7Y-FFW7-5WQA
更新日期：2005-06-01 00:00:00
abstract：:This, to authors' knowledge, is the first report in the United States of Tinea pedis caused by Trichophyton violaceum in a recent Southeast Asian immigrant. Because of the recent entry into this country of refugees from an area of the world where T. violaceum is endemic, this anthropophilic dermatophyte may become a s...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.1.110
更新日期：1983-07-01 00:00:00
abstract：:Pseudo-Pelger-Huët anomaly (PPHA) has been documented in association with transplant medications and other drugs. This iatrogenic neutrophilic dysplasia is reversible with cessation or adjustment of medications but is frequently confused with myelodysplastic syndrome (MDS) based on the conventional concept that PPHA i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPVFY95MAOBKRS
更新日期：2011-02-01 00:00:00
