Abstract：
:Clq precipitins were assayed in a model system by absorption nephelometry. Polyethylene glycol was used to extract immune complexes so that sufficient sensitivity with minimal interference could be achieved. Using a double beam spectrophotometer, we found that the reaction of immune complexes with Clq occurred rapidly and plateaued between 5-20 minutes. The optimal concentration of sodium in the reaction mixture was between 0.155-0.165 M, while chloride and EDTA did not affect the reaction significantly. Standard curves generated from heated aggregated gamma globulin showed a high degree of linearity, between 12.5-400 mg/L. Need for conductivity meters, radioisotopes, and other special equipment was eliminated because highly purified Clq is not required for nephelometric assay, and all necessary parameters can be monitored by flame photometry and electrophoresis, which are available in most clinical laboratories. Day to day reproducibility showed CVs of 5.4, 9.5, and 11.4% at control levels of 210, 160, and 70 mg/ L of immune complexes, respectively. Twelve patients with elevated levels of immune complexes by other methods were differentiated from normals by the present method. Nephelometry may offer a simplified approach for testing immune complexes and may become more widespread in clinical laboratories.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Levinson SS,Goldman Jdoi
10.1093/ajcp/79.4.451subject
Has Abstractpub_date
1983-04-01 00:00:00pages
451-7issue
4eissn
0002-9173issn
1943-7722journal_volume
79pub_type
杂志文章abstract：:An aggressive visceral form of Kaposi's sarcoma (KS) has been reported in immunosuppressed populations, most notably in patients with acquired immunodeficiency syndrome (AIDS). This presentation contrasts from the indolent skin lesions on the extremities of older males and is distinct enough in the young adult America...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.3.366
更新日期：1986-09-01 00:00:00
abstract：:Complete hydatidiform moles (CHM) and partial hydatidiform moles (PHM) represent different clinicopathologic entities. To obtain prognostic and therapeutic information about both entities, it is important that pathologic classification be as accurate as possible. The distinction of molar pregnancy and an abortus with ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/110.6.776
更新日期：1998-12-01 00:00:00
abstract：:This study sought to compare Multiplate impedance platelet aggregometry (IPA) with light transmission aggregometry (LTA) and the PFA-100 for determining the prevalence of residual platelet reactivity (RPR) by the Multiplate IPA in 297 patients with acute coronary syndrome receiving dual antiplatelet therapy. Aggregati...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPTE3K1SGAPOIZ
更新日期：2009-06-01 00:00:00
abstract：:Reported is a case of lymphoma which was preceded for nine years by an apparently reactive lymphadenopathy. Original slides of the multiple lymphoid tissue samples are reviewed, with emphasis on the gradually increasing numbers of mitoses, atypical histiocytes, and eosinophils, suggestive of malignant transformation. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.5.606
更新日期：1977-11-01 00:00:00
abstract：:Upon hospital admission a patient was found to have severe anemia and a strongly positive direct antiglobulin test (DAT). The patient was taking probenecid periodically for gout. An antibody was detected in the patient's serum that only reacted with red blood cells (RBCs) when probenecid was added. Eluates from the pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.3.391
更新日期：1985-09-01 00:00:00
abstract：:In the differential diagnosis of Spitz nevus vs malignant melanoma, patient age provides a critical piece of clinical information, because Spitz nevi occur mostly in children and melanomas occur mostly in adults. Nevertheless, there is overlap in the age distributions of Spitz nevus and melanoma. The issue to consider...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/E14C-J6KR-D092-DP3M
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVES:Although diffuse alveolar damage, a subtype of acute lung injury (ALI), is the most common microscopic pattern in coronavirus disease 2019 (COVID-19), other pathologic patterns have been described. The aim of the study was to review autopsies from COVID-19 decedents to evaluate the spectrum of pathology and ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa156
更新日期：2020-11-04 00:00:00
abstract：:After the March 2004 implementation of American Association of Blood Banks standards regarding platelet bacterial detection, we began quantitative glucose screening of whole blood-derived platelets (WB-P). The glucose level was measured immediately before component release--often storage day 4 or 5--using the Glucomet...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPVN9OT4GRCSKO
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVES:To characterize the clinical and pathologic features of mantle cell lymphoma with mantle zone growth pattern (MCL-MZGP). METHODS:The clinicopathologic data from 35 cases of MCL-MZGP obtained in 12 centers were analyzed. RESULTS:The patients with MCL-MZGP typically sought treatment at high clinical stages (...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz043
更新日期：2019-07-05 00:00:00
abstract：OBJECTIVES:Method imprecision, error rates, and explanatory causes that were identified in the Institute for Quality Management in Healthcare point-of-care (POC) glucose proficiency testing (PT) program were assessed in comparison with results obtained from laboratory glucose PT surveys. METHODS:POC and laboratory glu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPP5YS2MVSKBYY
更新日期：2014-12-01 00:00:00
abstract：:Anaplastic large cell lymphoma (ALCL) is clinically and pathologically heterogeneous, leading to some difficulty in its diagnosis and acceptance as a specific entity. ALCL can be subdivided into different groups according to histologic features (pleomorphic, monomorphic, small cell predominant, Hodgkin's disease-relat...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:The authors describe 11 patients with non-Hodgkin's lymphoma arising in the nasal cavity or paranasal sinuses. Seven were men and four were women. The median age of the women was 76 years; the median age of the men was 45 years. The most common presenting symptoms were nasal obstruction and unilateral facial swelling....
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.6.721
更新日期：1984-06-01 00:00:00
abstract：:The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPAH5WR2LFTUSI
更新日期：2011-12-01 00:00:00
abstract：:Little is known concerning the pathology of spleens removed for traumatic injury. The authors studied the gross and microscopic features of 44 spleens removed for trauma and received at the Surgical Pathology Division of Parkland Memorial Hospital and 10 normal control spleens from the Medical Examiner's Office, Dalla...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/105.4.474
更新日期：1996-04-01 00:00:00
abstract：:A simple scheme for the speciation of clinical isolates of "viridans" streptococci based on nine physiological characteristics is presented. Use of this scheme will enable successful speciation of most "viridans" strep isolates in any laboratory and allow for the accumulation of data necessary for the definition of cl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.1.78
更新日期：1982-07-01 00:00:00
abstract：:Acute myeloid leukemia (AML) with isolated trisomy 4 is rare. Associations with KIT mutations on chromosome 4q12 have been documented. The clinicopathologic features and mutational status of KIT, FLT3, NPM1, CEBPA, and RAS were assessed in 13 AML cases with isolated trisomy 4. There were 9 men and 4 women with a media...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP7ZC9YQERSKGX
更新日期：2012-03-01 00:00:00
abstract：:HER2 overexpression and amplification have been studied as a therapeutic and prognostic target in a number of human cancers, including esophageal, gastric, and colorectal adenocarcinomas. However, HER2 status has not been well investigated in primary small intestinal adenocarcinoma, probably because of its rarity. In ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPK6QHNNOEMJIM
更新日期：2010-12-01 00:00:00
abstract：:Seventy-nine mycelial-form stock cultures of Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, and morphologically similar fungi were extracted and tested by using commercial macroimmunodiffusion exoantigen test kits and the Centers for Disease Control (CDC) reference system for identifying funga...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.3.379
更新日期：1983-03-01 00:00:00
abstract：OBJECTIVES:We report a case of a patient with diabetes mellitus and unexpectedly low hemoglobin A1c results associated with paroxysmal nocturnal hemoglobinuria (PNH). We review the impact of shortened RBC half-life on the interpretation of hemoglobin A1c levels. METHODS:Patient history and laboratory test results were...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPTK1HPR2KULJU
更新日期：2014-08-01 00:00:00
abstract：:A man was hospitalized with bacteremic Achromobacter xylosoxidans type IIIa pneumonia. The authors are aware of no previously reported similar infections caused by this bacterium. A clinical cure was achieved with a combination of carbenicillin and kanamycin therapy. Microtiter susceptibility testing revealed that car...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.4.712
更新日期：1978-10-01 00:00:00
abstract：:In 1985 Harvard Medical School initiated an experimental curriculum that incorporated many of the recommendations of the report on the General Professional Education of the Physician (GPEP). Key features are problem-based small group tutorials that emphasize active learning, with increased independent study time and a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Possible increased activation of the coagulation pathway was measured in a group of patients with neoplastic diseases. In addition to standard tests, the thromboplastin generation test, thrombin generation test and immunologic and coagulant activities of both Factor VIII and antithrombin III were utilized in the evalu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.2.258
更新日期：1977-08-01 00:00:00
abstract：:Intrauterine clostridial infections have historically been associated with puerperal sepsis, often subsequent to instrumented abortions. Isolated reports have been associated with a malignant neoplasm or degenerating leiomyoma, most often after instrumentation, radiation therapy, or chemotherapy. In these cases, well-...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.1.104
更新日期：1989-01-01 00:00:00
abstract：:The levels of thymus-derived lymphocytes (T-cells) in samples of peripheral blood from 47 patients with carcinoma of the breast, 36 patients with benign mammary lesions, and 33 matched normal controls were determined. Results were analyzed with three statistical tests. It was found that the mean percentages of T-lymph...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/65.4.445
更新日期：1976-04-01 00:00:00
abstract：:The authors investigated assays for free protein S (ProS) antigen, total ProS antigen, and ProS crossed immunoelectrophoresis (CIEP) in the diagnosis of type 1 inherited ProS deficiency. Accurate measurement of the hemostatically important free ProS required showing that, on each specimen, precipitation of the C4b-bin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.2.176
更新日期：1990-08-01 00:00:00
abstract：:One hundred fifty-two patients with amyloid in the tenosynovium who had carpal tunnel release were identified. Twenty-eight patients were excluded because of systemic amyloidosis: primary systemic amyloidosis (AL) in 24, secondary amyloidosis (AA) in 3, and familial amyloidosis (AF) in 1. The remaining 124 patients (8...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/91.4.393
更新日期：1989-04-01 00:00:00
abstract：:This study aimed to ascertain pathologic findings of surgical follow-up excision (FUE) on patients who had radiologic finding of calcifications and lobular neoplasia (LN) on core biopsy. Breast core biopsy specimens from 2006-2011 with a diagnosis of pure classic-type LN (lobular carcinoma in situ [LCIS] and atypical ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPYG48TUTFIBMR
更新日期：2012-07-01 00:00:00
abstract：:Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/7Y7N-M1WM-R0YK-M2VA
更新日期：2002-05-01 00:00:00
abstract：:The present report is that of a civilian episode of fatal, fulminant group Y meningococcemia in a previously healthy adolescent, who denied prior vaccination against group C meningococcus. The patient suffered abrupt onset of purpura, hypotension and cardiopulmonary arrest. A detailed clinical and pathologic report is...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.2.296
更新日期：1977-08-01 00:00:00
abstract：:Loss of intercellular adhesion facilitates tumor invasion. To clarify the relation between altered expression of cell adhesion molecules and progression of T1 superficial bladder tumors, 101 cases (71 T1 tumors, 30 T2/T3 tumors) were examined immunohistochemically for E-cadherin and alpha-, beta-, and gamma-catenins. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
