Evaluation of a commercial exoantigen test system for the rapid identification of systemic fungal pathogens.

Abstract:

:Seventy-nine mycelial-form stock cultures of Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, and morphologically similar fungi were extracted and tested by using commercial macroimmunodiffusion exoantigen test kits and the Centers for Disease Control (CDC) reference system for identifying fungal isolates. Results showed 100% correlation between the two systems. Specific exoantigens of C. immitis and H. capsulatum extracted from agar slant cultures (slant extraction method) readily were identified. In eight of 26 cultures of B. dermatitidis, broth culture filtrates (broth-shake-flask method) were required to demonstrate the specific bands of identity. No false-negative reactions or cross-reactivity among the pathogens and other fungi were observed. The commercial test kits provided a rapid and specific method for identifying or confirming suspected fungal pathogens.

journal_name

Am J Clin Pathol

authors

Denys GA,Newman MA,Standard PG

doi

10.1093/ajcp/79.3.379

subject

Has Abstract

pub_date

1983-03-01 00:00:00

pages

379-81

issue

3

eissn

0002-9173

issn

1943-7722

journal_volume

79

pub_type

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