Determination of proliferation index in advanced ovarian cancer using quantitative image analysis.

Abstract:

:It has been shown that the monoclonal antibody Ki-67 reacts with a nuclear antigen that is expressed only by proliferating cells. The feasibility of using image analysis to quantitate Ki-67 staining (proliferation index [PI]) of epithelial ovarian cancers was investigated. The PI was determined in 50 advanced-stage primary ovarian cancers. Frozen sections were immunostained with the Ki-67 monoclonal antibody, and the PI was calculated using static image analysis. Among 35 stage III ovarian carcinomas, the median PI was 8.9%, compared with 17.7% in 15 stage IV cancers (P = 0.06). There was no relationship between PI and histologic grade. The median survival time of 32 patients whose cancers had a high Ki-67 expression (> or = 7.5%) was 16.8 months, which differed significantly (P < 0.01) from the median survival of 31.5 months observed in patients whose tumors demonstrated low Ki-67 expression (< 7.5%). Quantitative image analysis of Ki-67-stained fresh-frozen ovarian cancers may provide useful prognostic information. Further studies are warranted to investigate the relationship between Ki-67 expression and other known clinicopathologic and genetic features of ovarian cancer.

journal_name

Am J Clin Pathol

authors

Jordan PA,Kerns BJ,Pence JC,Kohler MF,Bast RC Jr,Kinney RB,Berchuck A

doi

10.1093/ajcp/99.6.736

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

736-40

issue

6

eissn

0002-9173

issn

1943-7722

journal_volume

99

pub_type

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