The utility of BRAF V600E mutation-specific antibody VE1 for the diagnosis of hairy cell leukemia.

Abstract:

OBJECTIVES:BRAF V600E mutation is characteristic of hairy cell leukemia (HCL). A V600E mutation-specific antibody, VE1, has been recently developed. We studied the diagnostic utility of this antibody in HCL and compared it with other B-cell neoplasms. METHODS:VE1 activity was assessed using immunohistochemistry in 90 mature B-cell neoplasms, including HCL (n = 17), HCL variant (n = 6), chronic lymphocytic leukemia (CLL) (n = 20), and 47 other B-cell lymphomas. Most (87/90) specimens were formalin-fixed, paraffin-embedded bone marrow (BM) biopsy specimens decalcified in either hydrochloric acid or formic acid. RESULTS:VE1 was positive in 15 (88%) cases of HCL and two (10%) cases of CLL and was negative in all other tumors assessed. The VE1-positive HCL cases showed uniform staining in all tumor cells, but intensity was variable. The two VE1-negative HCL cases had BRAF V600 mutations proven by molecular analysis. The two CLL cases positive with VE1 showed an atypical staining pattern with expression in a minority of lymphoma cells. Immunohistochemistry using the VE1 antibody had a sensitivity of 88% and a specificity of 97% for HCL. CONCLUSIONS:VE1 immunohistochemistry is a useful and convenient surrogate for detecting BRAF V600E mutation in BM biopsy specimens decalcified with hydrochloric or formic acid-based solutions.

journal_name

Am J Clin Pathol

authors

Uppal G,Ly V,Wang ZX,Bajaj R,Solomides CC,Banks PM,Medeiros LJ,Peiper SC,Gong JZ

doi

10.1309/AJCPQLQ89VXTVWKN

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

120-5

issue

1

eissn

0002-9173

issn

1943-7722

pii

143/1/120

journal_volume

143

pub_type

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