Abstract:
OBJECTIVES:BRAF V600E mutation is characteristic of hairy cell leukemia (HCL). A V600E mutation-specific antibody, VE1, has been recently developed. We studied the diagnostic utility of this antibody in HCL and compared it with other B-cell neoplasms. METHODS:VE1 activity was assessed using immunohistochemistry in 90 mature B-cell neoplasms, including HCL (n = 17), HCL variant (n = 6), chronic lymphocytic leukemia (CLL) (n = 20), and 47 other B-cell lymphomas. Most (87/90) specimens were formalin-fixed, paraffin-embedded bone marrow (BM) biopsy specimens decalcified in either hydrochloric acid or formic acid. RESULTS:VE1 was positive in 15 (88%) cases of HCL and two (10%) cases of CLL and was negative in all other tumors assessed. The VE1-positive HCL cases showed uniform staining in all tumor cells, but intensity was variable. The two VE1-negative HCL cases had BRAF V600 mutations proven by molecular analysis. The two CLL cases positive with VE1 showed an atypical staining pattern with expression in a minority of lymphoma cells. Immunohistochemistry using the VE1 antibody had a sensitivity of 88% and a specificity of 97% for HCL. CONCLUSIONS:VE1 immunohistochemistry is a useful and convenient surrogate for detecting BRAF V600E mutation in BM biopsy specimens decalcified with hydrochloric or formic acid-based solutions.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Uppal G,Ly V,Wang ZX,Bajaj R,Solomides CC,Banks PM,Medeiros LJ,Peiper SC,Gong JZdoi
10.1309/AJCPQLQ89VXTVWKNsubject
Has Abstractpub_date
2015-01-01 00:00:00pages
120-5issue
1eissn
0002-9173issn
1943-7722pii
143/1/120journal_volume
143pub_type
杂志文章abstract::Whipple disease is a rare, chronic multisystem disease associated with the recently characterized organism Tropheryma whippelii. Extraintestinal manifestation involving the central nervous system, heart, and joints occasionally occurs. Involvement of the abdominal lymph nodes, especially the mesenteric and periaortic ...
journal_title:American journal of clinical pathology
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doi:10.1309/7678-E2DW-HFJ5-QYUJ
更新日期:2001-12-01 00:00:00
abstract::A three-and-a-half-year old boy suffering from streptococcal pneumonia developed hemolytic-uremic syndrome and disseminated intravascular coagulation (DIC). His red blood cells (RBC) were shown to be T- and Tk-activated; serial testing showed his mature RBCs as well as neocytes remained T-activated at 40 days. Anti-T ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.2.244
更新日期:1985-08-01 00:00:00
abstract::Squamous cell carcinoma (SCC) is by far the most common malignancy of the upper aerodigestive tract. Most conventional-type SCCs do not present the surgical pathologist with diagnostic difficulty. Certain variants, however, can histologically mimic benign and other malignant diseases. In such cases, correct diagnosis ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
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更新日期:2005-12-01 00:00:00
abstract::This article describes the seventh known case of blue nevus of the lymph node capsule and reviews the previously reported cases. The migration arrest hypothesis as an explanation for the occurrence of this lesion is favored rather than the "benign metastasis" hypothesis. The importance of the recognition of this pheno...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.3.367
更新日期:1984-03-01 00:00:00
abstract::The authors analyzed the frequency of immunophenotypic abnormalities in 1,474 cases of routinely fixed, paraffin-embedded B-lineage non-Hodgkin's lymphomas. B-lineage was determined by immunoreactivity for CD20 (L26, 92%); CD45RA (4KB5, an additional 3%) or immunoglobulin (Ig) light chain restriction (remaining 5%). C...
journal_title:American journal of clinical pathology
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abstract::Monoclonal immunoradiometric assays (IRMA) for human chorionic gonadotropin (hCG) are available for either intact hCG (IhCG) or beta subunit hCG (beta hCG). The authors evaluated the clinical applications of both methods. Serum samples (N = 180) were divided into the following five clinical groups: Group 1: elevated l...
journal_title:American journal of clinical pathology
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abstract::Reducing the rate of avoidable errors is crucial to patient safety. Telephone calls with misunderstood critical results constitute one area in which opportunities for improvement exist. The aviation industry has dealt with this issue by requiring pilots to repeat instructions received from the air traffic controller. ...
journal_title:American journal of clinical pathology
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更新日期:2004-06-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1995-03-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1980-11-01 00:00:00
abstract::A case of unilateral gonadoblastoma in association with a ruptured ectopic tubal pregnancy is presented. The patient had normal menstrual history prior to a missed period followed by abdominal pain and the passage of clots. The surgical specimens, in addition to the ruptured tubal pregnancy, showed nodular foci of typ...
journal_title:American journal of clinical pathology
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pub_type: 杂志文章,随机对照试验
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更新日期:2017-11-20 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1995-09-01 00:00:00
abstract::During a 14-year period (1959-1972), 3,600 endometrial suction biopsies were performed in the office for specific indications in selected patients. Three hundred seventy-seven biopsies (10.5%) revealed abnormal findings. Of these, 233 showed endometritis, 96 associated with necrotic decidual or placental tissue, or bo...
journal_title:American journal of clinical pathology
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doi:10.1093/ajcp/63.3.9
更新日期:1975-01-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1999-02-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2006-08-01 00:00:00
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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更新日期:1990-03-01 00:00:00
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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更新日期:1993-09-01 00:00:00
abstract::We undertook a retrospective analysis of colonic spirochetosis in 14 cases: females, 3; males, 11; children, 4; adults, 10. Two men had HIV infections. All children and both HIV-infected men had abdominal complaints, diarrhea, or both. Most other adults underwent colonoscopy for polyp screening (n = 4) or follow-up of...
journal_title:American journal of clinical pathology
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更新日期:2003-12-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1984-05-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:2006-03-01 00:00:00
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journal_title:American journal of clinical pathology
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更新日期:1986-01-01 00:00:00
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