Abstract：
:Pathologists are expected to accurately diagnose increasingly smaller breast carcinomas. Correct classification (ie, lobular vs ductal or in situ vs invasive) directly affects subsequent management, especially when the focus is near a surgical margin or present in a needle core biopsy and is further challenging if the lesion is morphologically ambiguous. We assessed the diagnostic utility of a multiplex, trichromogen immunostain of 3 commonly employed antibodies (CK7, p63, and E-cadherin) developed in our laboratory to evaluate these small lesions. Of the 147 specimens containing minimal (defined as ≤3 mm in size) invasive carcinoma, 81 also contained in situ carcinoma. In each case, the Minimal Carcinoma Triple Stain was prepared with a parallel H&E-stained slide. Observations of staining characteristics in the focus of interest were recorded. The Minimal Carcinoma Triple Stain was diagnostically useful in all but 1 case. In a case of invasive lobular carcinoma in an excisional biopsy, the Minimal Carcinoma Triple Stain stained only the surrounding breast tissue (appropriately) and not the focus of interest. Also, a subset of 29 of 81 excisional biopsies had minimal invasive carcinoma located 2 mm or less from the inked surgical margin, in which in all cases the Minimal Carcinoma Triple Stain was fully interpretable despite morphologic distortion due to concomitant cautery artifact and tissue disruption in some cases. The Minimal Carcinoma Triple Stain offers an accurate and tissue-conserving method to diagnose small, morphologically problematic foci of breast carcinoma while ideally leaving more tissue for additional adjunctive studies.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Ross DS,Liu YF,Pipa J,Shin SJdoi
10.1309/AJCPF4O0ADKFMGRJsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
62-70issue
1eissn
0002-9173issn
1943-7722pii
139/1/62journal_volume
139pub_type
杂志文章abstract：:Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP m...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.2.237
更新日期：1984-02-01 00:00:00
abstract：:Cervical cone biopsy has become an important surgical procedure for endocervical adenocarcinoma in situ (AIS), especially for patients who desire to retain their fertility. Establishing the usefulness of the endocervical margin status in cone biopsy specimens as a predictor of residual AIS is paramount. We examined th...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/109.6.727
更新日期：1998-06-01 00:00:00
abstract：OBJECTIVES:Overuse of laboratory tests is a persistent issue. We examined the use and overuse of serum immunofixation electrophoresis and serum free light chain assays to develop an algorithm for optimizing utilization. METHODS:A retrospective review of all tests, for investigation of monoclonal gammopathies, for all ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw026
更新日期：2016-04-01 00:00:00
abstract：:An evaluation of the performance of duplicate prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays was undertaken to develop analytical duplicate performance criteria in order to quantitate the risks associated with singlet versus duplicate procedures. Data were retrospectively collected from ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.4.456
更新日期：1986-04-01 00:00:00
abstract：OBJECTIVES:Nucleic acid amplification tests on formalin-fixed, paraffin-embedded (FFPE) tissue specimens enable Mycobacterium tuberculosis complex (MTB) detection and rapid tuberculosis diagnosis in the absence of microbiologic culture tests. We aimed to evaluate the efficacy of different polymerase chain reaction (PCR...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP2QZRH4ZNPRDD
更新日期：2014-09-01 00:00:00
abstract：:A case of non-Hodgkin's lymphoma of T-cell type occurring in a renal transplant recipient is described. This lymphoma was classified as large cell, immunoblastic type and presented as a mediastinal mass, although it was demonstrated to be disseminated at autopsy two weeks later. Lymphoma cells expressed the immunologi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.2.280
更新日期：1990-02-01 00:00:00
abstract：OBJECTIVES:BCOR-CCNB3 sarcoma (BCS) is an undifferentiated tumor that has some clinical and morphologic similarity to classic Ewing sarcoma, but it is characterized by a distinct BCOR-CCNB3 gene fusion. There are no reports describing its cytomorphologic findings. METHODS:We describe cytologic findings of five molecul...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz159
更新日期：2020-02-08 00:00:00
abstract：:The authors describe an unusual variant of alkaline phosphatase (ALP) discovered in a patient with a 90-fold increase in serum ALP. The variant ALP was indistinguishable from the bone isoenzyme when subjected to chemical inhibition, heat inactivation, lectin precipitation, and routine electrophoresis. However, treatme...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.5.749
更新日期：1991-05-01 00:00:00
abstract：:Utilization of nonconventional bacterial strains and electron microscopy occasionally may aid in the recognition of unusual microorganisms which fail to be demonstrated by more conventional bacterial stains. We report an unusual case of recurrent vaginal polyps, initially thought by physical examination to represent a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.5.637
更新日期：1983-05-01 00:00:00
abstract：:Cultures from the gallbladder and blood of a 60-year-old man with acute cholecystitis grew Haemophilus aphrophilus. This organism, an unusual isolate in clinical specimens, is most frequently seen in patients with either endocarditis or brain abscesses. Haemophilus aphrophilus may be distinguished from Eikenella corro...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.1.361
更新日期：1978-03-01 00:00:00
abstract：:The Society for Hematopathology and European Association for Haematopathology workshop, from October 27 to 29, 2011, in Los Angeles, CA, exhibited many exemplary skin biopsy specimens with interesting inflammatory changes mimicking features of cutaneous lymphoma. This article reviews features observed in cutaneous lym...
journal_title：American journal of clinical pathology
pub_type： 共识发展会议,杂志文章
doi：10.1309/AJCPX4BXTP2QBRKO
更新日期：2013-04-01 00:00:00
abstract：:This study investigates the dispensing and inoculating characteristics of the MIC-2000 micro-broth dilution system and compares its ability to determine minimum inhibitory concentrations with that of the reference macro-broth dilution method. Micro-well filling showed a coefficient of variation of 6.8%, and micro-well...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.3.374
更新日期：1980-03-01 00:00:00
abstract：:The authors describe the case of a 33-year-old man with a solitary cystic lesion of the left distal radius with central radiodensities that radiographically suggested a chondroid matrix. Curettage of the lesion revealed numerous calcospherites with a lamellar structure. This lamellar pattern is thought to be a result ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/92.6.831
更新日期：1989-12-01 00:00:00
abstract：:Two cases of papillary cystic tumor (PCT) of the pancreas were investigated for the presence of estrogen receptors (ERs) and progesterone receptors (PgRs). Both PCT and normal pancreas are able to specifically bind 3H-estradiol. This binding almost exclusively results from the presence of high levels of type II ER, wh...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/92.5.572
更新日期：1989-11-01 00:00:00
abstract：:Cytology frequently has some role in preoperatively distinguishing pancreatic mucus-producing neoplasia (intraductal papillary mucinous neoplasms [IPMNs] and mucinous cystic neoplasms [MCNs]) from other pancreatic cysts. We evaluated all cytologic specimens at our institutions from resected pancreatic cystic lesions f...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/Y16QCXE3YGMXX19Q
更新日期：2008-01-01 00:00:00
abstract：:Trends in surgical practice suggest that pathologists will encounter increased numbers of patients with small invasive ductal adenocarcinomas; small, if any, metastatic deposits in axillary lymph nodes (ALNs); and possibly fewer ALN specimens to examine. New prognostic histologic features may be needed in this environ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/111.1.21
更新日期：1999-01-01 00:00:00
abstract：:The effect of blood transfusion on DNA profiles of an individual is a significant issue in the forensic context. In the present study, the effects of blood transfusion in 5 recipients were studied by performing restriction fragment length polymorphism and polymerase chain reaction HLA-DQA1 and Polymarker (LDLR, GYPA, ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/KAWA-QHBU-6D1J-XY3G
更新日期：2002-09-01 00:00:00
abstract：:Intrauterine pulmonary infarction is extremely rare. In a series of 775 stillborn and newborn infants, only a few cases of acute pulmonary infarction were reported. The authors present the findings from a full-term infant who died at 9 hours of age of a chronic pulmonary infarction. Several pathogenic mechanisms, incl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.6.701
更新日期：1993-12-01 00:00:00
abstract：:This article describes the seventh known case of blue nevus of the lymph node capsule and reviews the previously reported cases. The migration arrest hypothesis as an explanation for the occurrence of this lesion is favored rather than the "benign metastasis" hypothesis. The importance of the recognition of this pheno...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.3.367
更新日期：1984-03-01 00:00:00
abstract：:Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/6H9844AMMET82DBJ
更新日期：2008-07-01 00:00:00
abstract：:The use of rapid, simple tests to detect antibodies to human immunodeficiency virus (HIV) in urine could be valuable for several testing situations, such as in private offices, for epidemiologic surveys, and in developing countries. The authors evaluated the performance of the SUDS HIV type 1 test to detect antibody t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.2.157
更新日期：1994-02-01 00:00:00
abstract：Objectives:Diagnosis of B-cell acute lymphoblastic leukemia (B-ALL) requires immunophenotypic evidence of B-lineage and absence of specific myeloid or T-lineage markers. Rare cases of otherwise typical B-ALL express myeloperoxidase (MPO) detectable by flow cytometry with an absence of other myeloid markers, but the cli...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqx021
更新日期：2017-04-01 00:00:00
abstract：OBJECTIVES:Sentinel lymph node (SLN)-based diagnosis in gastric cancers has shown varied sensitivities and false-negative rates in several studies. Application of the reverse transcription-polymerase chain reaction (RT-PCR) in SLN diagnosis has recently been proposed. METHODS:A total of 155 SLNs from 65 patients with ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw055
更新日期：2016-05-01 00:00:00
abstract：:A case of florid proliferative sialometaplasia arising in salivary ductal inclusions in an intraparotid lymph node is described. This lesion probably represents an exuberant response to sialadenitis. Other entities that enter into its differential diagnosis in this particular location are discussed. Except for Warthin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.1.116
更新日期：1986-07-01 00:00:00
abstract：:While plasmacytosis is a common occurrence in early post-bone marrow transplantation biopsy specimens, the significance of plasma cells in such specimens from patients with multiple myeloma (MM) is unknown. We attempted to retrospectively determine, by morphologic assessment of plasma cell percentage, immunohistologic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.6.643
更新日期：1997-06-01 00:00:00
abstract：:A nodule of the liver was incidentally noted during left colectomy for a large villous adenoma in a 74-year-old man. Microscopic examination disclosed a granuloma of the liver containing numerous Enterobius vermicularis ova and a cross-section of the nematode. This is the fourth reported case of hepatic oxyuriasis and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.1.97
更新日期：1989-01-01 00:00:00
abstract：:Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.4.517
更新日期：1991-04-01 00:00:00
abstract：:Because T-regulatory cells (Tregs) can harmfully impair HIV-specific responses or beneficially limit immune activation, we compared the number of Tregs in lymph nodes from 48 HIV+ patients and 106 HIV- subjects. By using a microscope counting grid, we found that the mean ± SD number of Tregs in lymph nodes was 3 times...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPAR39BFWNHWRO
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVES:Edoxaban, an oral direct factor Xa inhibitor, does not require routine monitoring. However, assessment of the anticoagulant effects may be required in certain situations. METHODS:We investigated the effects of edoxaban on prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin gene...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPQ2NJD3PXFTUG
更新日期：2015-02-01 00:00:00
abstract：:We previously reported 24 cases of marrow fibrin-ring granuloma (FRG) encountered in 1 institution and concluded that, contrary to previous studies showing marrow FRG as a diagnostic marker for Q fever, Epstein-Barr virus (EBV) was the most common proven cause of such FRG. The present study characterized patients with...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPB7SX7QXASPSK
更新日期：2010-02-01 00:00:00
