Abstract：
:Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP maintained viability of C. trachomatis to the greatest extent for all of the time intervals and temperatures examined. Therefore, in an attempt to further stabilize the viability of C. trachomatis, 2-SP was supplemented with various concentrations of bovine serum albumin (BSA) or fetal bovine serum (FBS). For the times and temperatures examined, 2-SP supplemented with 20% or 40% FBS or 5% BSA preserved infectivity to a greater extent than unsupplemented 2-SP. In some supplemented media, up to 65% of the infectivity was preserved after one week of storage at -176 degrees C, whereas only 0-3% of infectivity remained when stored in unsupplemented media at -20 degrees C and 4 degrees C, respectively. Therefore, supplementation of 2-SP with FBS or BSA can prolong the survival of chlamydia, which is critical in the transportation and storage of clinical specimens. In addition, storage for prolonged periods of time should be at -70 degrees C or lower temperatures.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Aarnaes SL,Peterson EM,De La Maza LMdoi
10.1093/ajcp/81.2.237subject
Has Abstractpub_date
1984-02-01 00:00:00pages
237-9issue
2eissn
0002-9173issn
1943-7722journal_volume
81pub_type
杂志文章abstract：:This study assessed the value of argyrophilic nucleolar organizer region (AgNOR) staining as a potential technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Using a silver staining technique and immunohistochemistry, the authors correlated the numbe...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/92.4.401
更新日期：1989-10-01 00:00:00
abstract：:The authors investigated the blast cells obtained from two patients with acute myelofibrosis with the use of recently developed immunochemical and ultrastructural markers. They intended to examine the expression of megakaryoblastic, erythroblastic, myeloblastic, and monoblastic properties in these cells. Respectively,...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/83.1.114
更新日期：1985-01-01 00:00:00
abstract：:Sera have been submitted to the authors' laboratory for syphilis testing at a dramatically increased rate since the start of the human immunodeficiency virus type 1 epidemic. Thus they were interested in evaluating a new enzyme immunoassay, the DCL Syphilis-G test kit (DCL-G, Diagnostic Chemicals Ltd., Oxford, CT) for...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.3.282
更新日期：1993-03-01 00:00:00
abstract：:The authors describe 11 patients with non-Hodgkin's lymphoma arising in the nasal cavity or paranasal sinuses. Seven were men and four were women. The median age of the women was 76 years; the median age of the men was 45 years. The most common presenting symptoms were nasal obstruction and unilateral facial swelling....
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.6.721
更新日期：1984-06-01 00:00:00
abstract：:Pulmonary alveolar proteinosis (PAP) is an uncommon disease in which alveoli are progressively filled with surfactant-related material. Although a definitive diagnosis is usually made by an open lung biopsy, bronchoalveolar lavage (BAL) cytology may play a decisive role in the clinical work-up of these patients, and, ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/106.4.504
更新日期：1996-10-01 00:00:00
abstract：:Increasingly, all automated blood counts are not accompanied by a microscopic white blood cell differential. A popular strategy is to obtain a manual differential if any part of the automated blood count and differential is outside specified limits (the "diff-if" strategy). The authors compared two sets of criteria to...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.2.163
更新日期：1988-08-01 00:00:00
abstract：:An evaluation of the validity in infants of standard computations for the correction of white cell counts in blood-contaminated cerebrospinal fluid (traumatic lumbar puncture) was undertaken. In 39 infants with significant blood contamination (greater than 10(4) red blood cells/microL) and no evidence of bacterial men...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/82.1.95
更新日期：1984-07-01 00:00:00
abstract：:An acquired circulating inhibitor to Factor VIII:C was found in a 27-year-old postpartum woman who presented with ecchymoses and hematomas. Postpartum Factor VIII:C inhibitors can clinically manifest with signs and symptoms not unlike those in a classic hemophiliac. The natural history of this inhibitor is typically o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.5.789
更新日期：1982-11-01 00:00:00
abstract：:A goat anti-serum was prepared against mucinous ovarian cyst fluid and absorbed with normal colon and a variety of normal tissues until the only residual immunoreactivity was directed against colon cancer and ovarian tumor mucin. The set of antigenic determinants defined by this anti-serum has been called COTA, standi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.1.1
更新日期：1986-07-01 00:00:00
abstract：:Twenty-eight resected primary non-small cell lung carcinomas were studied for intratumoral DNA variability by flow cytometry (FCM). Three separate tissue specimens from each resected tumor were divided equally for FCM analysis and histologic evaluation. FCM analysis also was performed on fine-needle aspirates (FNAs) f...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.3.311
更新日期：1991-09-01 00:00:00
abstract：:Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/74.4.371
更新日期：1980-10-01 00:00:00
abstract：:Remarkable improvements have been made in blood safety since the onset of the HIV epidemic. However, the current paradigm does not prevent all transfusion-transmitted infections and is reactive to new agents, thus accepting that some patients may be harmed before preventive measures are introduced. Several methods are...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/RAPQ3NXG3MV9AL94
更新日期：2007-12-01 00:00:00
abstract：:The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.6.687
更新日期：1990-12-01 00:00:00
abstract：:To verify their clinical usefulness in diagnosis and early response to therapy of sideropenic anemia, we compared the behavior of the reticulocyte parameter (RET-Y), a raw measure dependent on size and content of the cell, generated by the Sysmex XE 2100, with the mean reticulocyte volume (MCVr) and mean reticulocyte ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/W652-95DT-UWK7-U1HH
更新日期：2004-04-01 00:00:00
abstract：:Screening for serum lipid disorders is recommended by numerous specialty societies to identify patients at risk for coronary heart disease (CHD). The best screening tests will identify patients at highest risk for CHD who would benefit from intervention. This report discusses an appropriate test panel to use as the in...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.4.380
更新日期：1995-04-01 00:00:00
abstract：OBJECTIVES:Fundic gland polyps (FGPs) occur in two clinical settings, sporadic and syndromic. Epithelial dysplasia is rare in sporadic FGPs, and progression data from large series are lacking. The aim of this study was to evaluate the clinical, morphologic, and immunohistochemical features in a large series of sporadic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPGK8QTYPUQJYL
更新日期：2015-10-01 00:00:00
abstract：:Double immunostaining of tissue sections by monoclonal antibodies has been performed using, in immunofluorescence, combinations of reagents conjugated with different fluorochromes. These were monoclonal antibodies directly labeled with phycoerythrin (a new red fluorochrome) and haptenated monoclonal antibodies detecte...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/82.1.44
更新日期：1984-07-01 00:00:00
abstract：:Posttransplantation lymphoproliferative disorders (PT-LPDs) occurring in T-cell depleted (TCD) allogeneic bone marrow transplant recipients seem to be different from those that arise in solid organ recipients in their early development, the high incidence of extensive dissemination at presentation, and their aggressiv...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.4.419
更新日期：1997-04-01 00:00:00
abstract：:A primary hepatic tumor with histologic features suggestive of carcinoid tumor is reported, and six previously reported cases reviewed. Tumor cells contained electron dense secretory granules with an average diameter 150-200 nm which demonstrated positive argyrophilic reactions. On immunohistochemistry, chromogranin A...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.4.561
更新日期：1988-04-01 00:00:00
abstract：:Immunoperoxidase technics were used to examine the immunoglobulin content of sections of pulmonary tissue from two typical cases of lymphomatoid granulomatosis and two cases of pneumonic processes initially diagnosed as lymphomatoid granulomatosis but representing different processes on review. Both "typical" cases an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.1.41
更新日期：1980-01-01 00:00:00
abstract：:To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.1.15
更新日期：1991-07-01 00:00:00
abstract：:The cytologic features of fine-needle aspiration specimens from 16 breast carcinomas that closely simulated benign lesions were analyzed and compared to smears from fibroadenomas and fibrocystic change. No combination of features was found that accurately separated all benign and malignant cases. Many nuclei with disc...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/98.2.155
更新日期：1992-08-01 00:00:00
abstract：:A 63-year-old previously healthy woman presented with acute meningitis. Cultures of the cerebrospinal fluid yielded a serotype 1/2a isolate of Listeria monocytogenes that was biochemically typical in all respects, other than the reproducible lack of catalase production. During therapy, the patient developed oculomotor...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.1.130
更新日期：1991-07-01 00:00:00
abstract：:To investigate the association between the positive direct antiglobulin test (DAT) and hypergammaglobulinemia, the authors prospectively studied 154 patients, classified into three groups: Group 1, 52 patients with a positive DAT result in pretransfusion samples; Group 2, 52 patients with a negative DAT result; and Gr...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.2.197
更新日期：1988-08-01 00:00:00
abstract：:Because of the unusual presentation of pernicious anemia in many black women, a further characterization of this disease among the different racial groups was undertaken. The following were found. (1) Black women under the age of 50 years had a significantly lower prevalence of anti-parietal cell antibody than did "Eu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.3.291
更新日期：1981-03-01 00:00:00
abstract：:A monoclonal antibody prepared against estrogen receptor has been shown to be specific and sensitive for the detection of estrogen receptor in human breast lesions by use of immunohistochemical methods. Two hundred selected cases of primary breast carcinoma were assayed for estrogen receptor content by biochemical and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.3.233
更新日期：1988-09-01 00:00:00
abstract：:Eight cases are reported of an unusual breast lesion consisting of dense intralobular, perilobular, and perivascular lymphocytic infiltrates associated with lobular atrophy and sclerosis. Immunologic studies on paraffin-embedded tissue (seven cases) and frozen tissue (two cases) demonstrated a predominance of B-cells ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.6.725
更新日期：1990-06-01 00:00:00
abstract：OBJECTIVES:Promotion of high-quality care at a lower cost requires educational initiatives across the continuum of medical education. A needs assessment was performed to inform the design of an educational tool with the goal of teaching laboratory stewardship to medical students. METHODS:The needs assessment consisted...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz102
更新日期：2020-01-01 00:00:00
abstract：:The "floral" variant of follicle center lymphoma (FCL) may be confused with progressive transformation of germinal centers or lymphocyte predominance Hodgkin lymphoma. Immunohistochemistry and gene rearrangement studies are usually sufficient to differentiate among these entities. We present 11 cases of floral FCL tha...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/V6PJ-BDAP-F0LU-CB6T
更新日期：2000-12-01 00:00:00
abstract：:"Low-grade squamous intraepithelial lesion (LSIL), cannot exclude high-grade squamous intraepithelial lesion" (LSIL-H) is an increasingly used, equivocal interpretive category in gynecologic cytology. In an effort to evaluate its potential usefulness as a measure of quality assurance, we studied patterns of use of the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP8J4ROAVWOSHQ
更新日期：2012-08-01 00:00:00
