Double immunostaining of lymph node sections by monoclonal antibodies using phycoerythrin labeling and haptenated reagents.

abstract：:A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...

journal_title：American journal of clinical pathology

authors： Ruebush TK 2nd,Goodman JS

更新日期：1975-10-01 00:00:00

abstract：:In today's environment, many laboratories and pathology practices are challenged to maintain or increase their quality while simultaneously lowering their overall costs. The cost of improving specimen processes is related to quality, and we demonstrate that actual costs can be reduced by designing "quality at the sour...

journal_title：American journal of clinical pathology

authors： Carlson RO,Amirahmadi F,Hernandez JS

更新日期：2012-09-01 00:00:00

abstract：:Pseudomonas aeruginosa is a frequent and virulent pulmonary pathogen in patients with cystic fibrosis. If colonization is not prevented, P aeruginosa becomes permanently established and nearly always mutates into a mucoid strain. The alginate-containing matrix of the mucoid strain is thought to allow the formation of ...

journal_title：American journal of clinical pathology

authors： Pritt B,O'Brien L,Winn W

更新日期：2007-07-01 00:00:00

abstract：:This is apparently the second report of a case of human meningoencephalitis caused by the nematode Micronema deletrix. The patient had severe granulomatous meningoencephalitis with numerous foci of hemorrhage and encephalomalacia. Numerous adult female and larval M. deletrix were in the brain. The organisms probably i...

journal_title：American journal of clinical pathology

authors： Shadduck JA,Ubelaker J,Telford VQ

更新日期：1979-10-01 00:00:00

abstract：:Heterogeneous expression or amplification is a challenge to HER2 diagnostics. A guideline defines heterogeneity as the presence of between 5% and 50% cells with HER2/CEP17 ratios of more than 2.20. We audited the frequency of such cells and their clinical impact in the results from 2 pathology laboratories combined wi...

journal_title：American journal of clinical pathology

authors： Bartlett AI,Starcyznski J,Robson T,Maclellan A,Campbell FM,van de Velde CJ,Hasenburg A,Markopoulos C,Seynaeve C,Rea D,Bartlett JM

更新日期：2011-08-01 00:00:00

abstract：OBJECTIVES:α1-Antitrypsin (AAT) deficiency is associated with an increased risk for lung and liver disease. Identification of AAT deficiency as the underlying cause of these diseases is important in correct patient management. METHODS:AAT deficiency is commonly diagnosed by demonstrating low concentrations of AAT foll...

journal_title：American journal of clinical pathology

authors： Suh-Lailam BB,Procter M,Krautscheid P,Haas J,Kumar S,Mao R,Grenache DG

更新日期：2014-05-01 00:00:00

abstract：:The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...

journal_title：American journal of clinical pathology

authors： Barry AL,Gavan TL,Badal RE,Telenson MJ

更新日期：1982-10-01 00:00:00

abstract：:Giant lymph node hyperplasia is uncommon in children and is exceptionally rare in infancy. The authors report a case of giant lymph node hyperplasia that presented as a right supraclavicular mass in an asymptomatic two-month-old female infant. Clinically, the mass was thought to be either a lipoma or a cystic hygroma,...

journal_title：American journal of clinical pathology

authors： Hunt SJ,Anderson WD

更新日期：1989-03-01 00:00:00

abstract：:Subsequent to recent disclosures that low serum vitamin B12 results can appear normal using many of the current radioassay methods because of the presence of cobalamin (vitamin B12) analogues in human serum, a number of radioassay kits have become available with modified intrinsic factor preparations and claims of mea...

journal_title：American journal of clinical pathology

authors： LeFebvre RJ,Virji AS,Mertens BF

更新日期：1980-08-01 00:00:00

abstract：:Location-guided screening in cervical cytology offers a potentially significant advance over routine manual screening. A prospective, 2-armed, masked clinical trial of the BD FocalPoint GS Imaging System using SurePath slides (BD Diagnostics-TriPath, Burlington, NC) compared routine manual screening and quality contro...

journal_title：American journal of clinical pathology

authors： Wilbur DC,Black-Schaffer WS,Luff RD,Abraham KP,Kemper C,Molina JT,Tench WD

更新日期：2009-11-01 00:00:00

abstract：:Immunohistochemistry occasionally is used to determine the lineage of entirely necrotic tumors. However, the sensitivity and specificity of antibodies on necrotic tissue are unknown. To determine the usefulness of immunohistochemistry with necrotic lesions, a series of 24 known tumors consisting of 14 carcinomas, 2 ly...

journal_title：American journal of clinical pathology

authors： Judkins AR,Montone KT,LiVolsi VA,van de Rijn M

更新日期：1998-11-01 00:00:00

abstract：:Three stillborn fetuses are reported in which an abdominal wall defect was associated with defects in the urogenital and anal region. Autopsy of these fetuses provided clues indicative of how and where the embryonic development went wrong. The malformation involved a disturbance of the cell deposition process, occurri...

journal_title：American journal of clinical pathology

authors： Hartwig NG,Steffelaar JW,Van de Kaa C,Schueler JA,Vermeij-Keers C

更新日期：1991-11-01 00:00:00

abstract：:This study focused on women who had an abnormal Papanicolaou smear and a subsequent colposcopic examination negative for a cervical squamous intraepithelial lesion. Cervical swabs obtained during the initial colposcopic examination were analyzed for human papillomavirus (HPV) DNA, and these results were correlated wit...

journal_title：American journal of clinical pathology

authors： Nuovo GJ,Moritz J,Walsh LL,MacConnell P,Koulos J

更新日期：1992-11-01 00:00:00

abstract：:Gc allele frequencies were determined in Haitian immigrants, a group at high risk for AIDS. Gc2, an allele associated with disease resistance, is infrequent in this group; Gc1f is frequent. ...

journal_title：American journal of clinical pathology

authors： Wenk RE,Russo F

更新日期：1988-09-01 00:00:00

abstract：:Three cases of pulmonary squamous cell carcinoma (SCC) are described displaying a prominent microcystic pattern closely resembling microcystic adnexal carcinoma of the skin (MAC). The patients were 2 women and 1 man aged 72 to 83 years. Histologically, in addition to conventional SCC, all tumors showed striking microc...

journal_title：American journal of clinical pathology

authors： Weissferdt A,Moran CA

更新日期：2011-09-01 00:00:00

abstract：:A new blood culture medium (16B) containing adsorbent and cationic exchange resins has become available for use with the BACTEC instrument (Johnston Laboratories, Towson, MD). Its purpose is to enhance the detection of bacteremia through binding of antimicrobials. The performance of the BACTEC 16B resin medium was com...

journal_title：American journal of clinical pathology

authors： DeGirolami PC,Eichelberger K,Siegel J

更新日期：1984-05-01 00:00:00

abstract：:To facilitate the differential diagnosis of poorly differentiated metastatic carcinomas of unknown primary site, we evaluated p63 and cytokeratin (CK) 5/6 as immunohistochemical markers for squamous cell carcinomas. The study cases were as follows: squamous cell carcinoma of the lungs, head/neck, esophagus, cervix ute...

journal_title：American journal of clinical pathology

authors： Kaufmann O,Fietze E,Mengs J,Dietel M

更新日期：2001-12-01 00:00:00

abstract：:A variant hemoglobin fraction may be an incidental finding during HbA(1c) analysis using the G8 Tosoh HPLC analyzer, but it is unclear if the retention times and fraction patterns can reliably predict the findings of a high-performance liquid chromatography (HPLC) β-thalassemia program (Bio Rad Variant II analyzer). W...

journal_title：American journal of clinical pathology

authors： Froom P,Henig C,Zalman L,Barak M

更新日期：2012-09-01 00:00:00

abstract：:Functional assays for heparin cofactor II (HC-II) are based on the inactivation of thrombin by HC-II in the presence of dermatan sulfate (DS). Residual thrombin is measured in a chromogenic assay. Interference by the antithrombin-III (AT-III)/heparin complex, which also rapidly inactivates thrombin, must be eliminated...

journal_title：American journal of clinical pathology

authors： Nakhleh R,Vogt JM,Edson JR

更新日期：1988-03-01 00:00:00

abstract：:Platelet volume measurements have been used to differentiate consumptive and hypoplastic thrombocytopenia. Since thrombocytopenia is a frequent complication of neonatal sepsis, the authors explored the utility of correlating mean platelet volume (MVP) and platelet distribution width (PDW) with bacteremia. In a sample ...

journal_title：American journal of clinical pathology

authors： Patrick CH,Lazarchick J

更新日期：1990-03-01 00:00:00

abstract：:A case of microcystic adenoma (glycogen-rich cystadenoma) of the exocrine pancreas in a 62-year-old white female presenting with adult-onset diabetes mellitus is reported. Clinical, histopathologic, and ultrastructural findings are discussed with particular emphasis on the presence of myoepithelial cells, a morphologi...

journal_title：American journal of clinical pathology

authors： Nyongo A,Huntrakoon M

更新日期：1985-07-01 00:00:00

abstract：:A murine monoclonal antibody (MAb 12C3) that is specific to human ovarian carcinomas was generated by immunizing mice with a human ovarian germinoma cell line (JOHYC-2). The antigen distribution that was defined by MAb 12C3 in normal and malignant human tissues was analyzed by immunohistochemistry on paraffin-embedded...

journal_title：American journal of clinical pathology

authors： Yamada K,Ohkawa K,Joh K

更新日期：1995-03-01 00:00:00

abstract：:A teenage male, with Goodpasture's syndrome and serum antiglomerular basement membrane (anti-GBM) antibodies, had a focal proliferative glomerulonephritis with crescents. Immunofluorescence microscopy of his glomeruli using anti-IgG antibodies demonstrated both intense linear GBM staining, and granular subepithelial s...

journal_title：American journal of clinical pathology

authors： Jennette JC,Lamanna RW,Burnette JP,Wilkman AS,Iskander SS

更新日期：1982-09-01 00:00:00

abstract：:Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...

journal_title：American journal of clinical pathology

authors： Elghetany MT,Kurec AS,Schuehler K,Forbes BA,Duggan DB,Davey FR

更新日期：1991-04-01 00:00:00

abstract：:Physicians are often forced to make decisions about the use of laboratory resources without adequate access to earlier results and related supporting information. Less than optimal use of the laboratory may result. The authors developed and deployed a clinical workstation meant to provide ready access to laboratory in...

journal_title：American journal of clinical pathology

authors： Connelly DP,Sielaff BH,Willard KE

更新日期：1995-09-01 00:00:00

abstract：:Coronary artery disease (CAD) is the leading cause of death in the United States. Increasing evidence suggests involvement of inflammation in the atherosclerotic process. We examined cytokines and other inflammatory markers in 865 patients with chest pain in whom coronary angiography revealed no evidence of CAD or CAD...

journal_title：American journal of clinical pathology

authors： Martins TB,Anderson JL,Muhlestein JB,Horne BD,Carlquist JF,Roberts WL,Carlquist JF

更新日期：2006-06-01 00:00:00

abstract：:Evaluation of blood cells to determine immunologic status is becoming an important clinical application of flow cytometric analysis. For a wider use of immunophenotyping technology in clinical laboratories, the authors developed a rapid method to detect monoclonal antibody-labeled cells using forward light scatter/abs...

journal_title：American journal of clinical pathology

authors： Kim YR,Paseltiner L,Kling G,Yeh CK

更新日期：1992-03-01 00:00:00

abstract：:Mean nuclear areas (MNA) were calculated morphometrically in ten cases of well-differentiated adenocarcinoma, ten cases of atypical alveolar cuboidal cell hyperplasia (AAH), and five cases of alveolar cuboidal cell hyperplasia (AH) and were compared with each other. In cases of adenocarcinoma, the MNA were significant...

journal_title：American journal of clinical pathology

authors： Kodama T,Biyajima S,Watanabe S,Shimosato Y

更新日期：1986-02-01 00:00:00

abstract：:A flow cytometric technic was developed to detect platelet surface-bound immunoglobulin in patients with thrombocytopenia. Elevated platelet surface IgG and/or IgM was detected in 90.9% of patients with immune thrombocytopenia purpura (ITP). False positive results occurred in 9.3% of patients with nonimmune thrombocyt...

journal_title：American journal of clinical pathology

authors： Rosenfeld CS,Nichols G,Bodensteiner DC

更新日期：1987-04-01 00:00:00

abstract：:Fine-needle aspiration (FNA) of thyroid bed (TB) lesions is a common diagnostic modality in monitoring patients for recurrent cancer after a thyroidectomy. To elucidate the value of TB FNA, we reviewed our experience at The Johns Hopkins Hospital, Baltimore, MD. We identified 57 TB FNA specimens from 50 patients. Of t...

journal_title：American journal of clinical pathology

authors： Bishop JA,Owens CL,Shum CH,Ali SZ

更新日期：2010-08-01 00:00:00