Abstract:
:This study assessed the value of argyrophilic nucleolar organizer region (AgNOR) staining as a potential technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Using a silver staining technique and immunohistochemistry, the authors correlated the numbers of argyrophilic nucleolar organizer regions (AgNORs) and Ki67 scores in 70 breast carcinomas and 27 benign breast lesions. Epithelial cells in fibrocystic disease and fibroadenomas contained a mean of 2.65-6.8 small uniform AgNORs per cell, whereas malignant cells contained 4.6-26.9 frequently highly irregular AgNORs. In benign tissue, Ki67 scores ranged from 0 to 4%; in malignant tumors, Ki67 scores ranged from 3.0 to 98%. The correlation between AgNOR counts and Ki67 scores was highly significant (P less than 0.001). The authors concluded that AgNOR counts performed on routine formalin-fixed paraffin sections furnish significant kinetic information. Furthermore, the difference in AgNOR counts between benign and malignant tumors is such that they may be of diagnostic value.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Dervan PA,Gilmartin LG,Loftus BM,Carney DNdoi
10.1093/ajcp/92.4.401subject
Has Abstractpub_date
1989-10-01 00:00:00pages
401-7issue
4eissn
0002-9173issn
1943-7722journal_volume
92pub_type
杂志文章abstract:OBJECTIVES:High-grade serous carcinoma (HGSC) is the most common ovarian malignancy. The role of cytopathology in obtaining tissue diagnosis before institution of neoadjuvant chemotherapy (NACT) was evaluated. METHODS:All histopathology-proven HGSC specimens between 2015 and 2018 with prior cytopathologic diagnosis by...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa028
更新日期:2020-06-08 00:00:00
abstract::A markedly elevated prothrombin time (PT) and activated partial thromboplastin time (APTT) were observed in a 24-year-old man who was admitted with a history of ethylene glycol ingestion. Further laboratory evaluation suggested that the coagulopathy was related to acquired factor deficiencies. The PT and APTT improved...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.6.663
更新日期:1995-12-01 00:00:00
abstract::This study was undertaken to evaluate the precision and sensitivity of three different commercial APTT reagents containing the activators kaolin, micronized silica, or ellagic acid. These reagents varied greatly in their ability to detect mild coagulopathies. The ellagic acid reagent was able to detect the mildest def...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.4.436
更新日期:1984-10-01 00:00:00
abstract::This study sought to compare Multiplate impedance platelet aggregometry (IPA) with light transmission aggregometry (LTA) and the PFA-100 for determining the prevalence of residual platelet reactivity (RPR) by the Multiplate IPA in 297 patients with acute coronary syndrome receiving dual antiplatelet therapy. Aggregati...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPTE3K1SGAPOIZ
更新日期:2009-06-01 00:00:00
abstract:OBJECTIVES:We report a case of a patient with diabetes mellitus and unexpectedly low hemoglobin A1c results associated with paroxysmal nocturnal hemoglobinuria (PNH). We review the impact of shortened RBC half-life on the interpretation of hemoglobin A1c levels. METHODS:Patient history and laboratory test results were...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCPTK1HPR2KULJU
更新日期:2014-08-01 00:00:00
abstract::The "floral" variant of follicle center lymphoma (FCL) may be confused with progressive transformation of germinal centers or lymphocyte predominance Hodgkin lymphoma. Immunohistochemistry and gene rearrangement studies are usually sufficient to differentiate among these entities. We present 11 cases of floral FCL tha...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/V6PJ-BDAP-F0LU-CB6T
更新日期:2000-12-01 00:00:00
abstract::Little information has been reported describing antigen stability in plasma cell myeloma. In this study, the expression frequency and stability of 2 potential therapeutic targets, CD20 and CD52, along with the frequently aberrantly expressed CD56 antigen, were evaluated by flow cytometric analyses in 56 patients with ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/8UVF7YQ1D4D4ETQV
更新日期:2008-06-01 00:00:00
abstract::Monoclonal antibody (MoAb) 44-3A6 recognizes a glandular differentiation-associated antigen and has been used to identify exocrine differentiation in pulmonary carcinomas. The authors assessed its value in the diagnosis of lung carcinomas metastatic to lung/pleura and pleural malignant mesothelioma (MM), using cell bl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.3.322
更新日期:1991-03-01 00:00:00
abstract::Medicare policies have gradually restricted the scope of pathologist services payable under the rules applicable to physician services. Recent changes applying relative value scales has provided an opportunity for national standardization of pathology codes, and has permitted the introduction of clinical pathology int...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::The authors investigated assays for free protein S (ProS) antigen, total ProS antigen, and ProS crossed immunoelectrophoresis (CIEP) in the diagnosis of type 1 inherited ProS deficiency. Accurate measurement of the hemostatically important free ProS required showing that, on each specimen, precipitation of the C4b-bin...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.2.176
更新日期:1990-08-01 00:00:00
abstract::Adenocarcinomas of the sigmoid colon and rectum were studied for the presence of blood group substances of the A, B, H system using the method of specific erythrocytic adherence. Of 17 adenocarcinomas studied, eight were found to have reactivity for blood group substances. All eight tumours showing reactivity for bloo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/69.6.594
更新日期:1978-06-01 00:00:00
abstract::Allergic adenoiditis is defined as the presence of numerous brightly fluorescent IgE mast cells (FIEMC) demonstrated in formalin-fixed adenoid tissue by the trypsin-immunofluorescent method. A prospective study of 606 routinely accessioned cases seen during a 39-month period revealed 98 positive cases. Correlation wit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.2.170
更新日期:1984-02-01 00:00:00
abstract:OBJECTIVES:To compare next-generation sequencing (NGS) platforms with mutation-specific analysis platforms in a clinical setting, in terms of sensitivity, mutation specificity, costs, capacity, and ease of use. METHODS:We analyzed 25 formalin-fixed, paraffin-embedded lung cancer samples of different size and tumor per...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP40XETVYAMJPY
更新日期:2015-04-01 00:00:00
abstract::A fatal case of an apparently congenital form of hemophagocytic reticulosis is reported. The onset was manifested by hyperbilirubinemia and hepatosplenomegaly which were present at birth and persisted throughout life. Fever, anemia and pancytopenia developed at 1 month of age and became progressively worse. A splenect...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.4.495
更新日期:1976-04-01 00:00:00
abstract::Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.3.512
更新日期:1976-09-01 00:00:00
abstract::Characteristically, mantle cell lymphoma (MCL) expresses surface immunoglobulin (sIg), CD19, CD20, and CD5 and lacks CD10 and CD23. Rare CD5-MCL variants have been described. This report describes a case of leukemic MCL with morphologically and immunophenotypically distinct classic MCL and blastoid-variant MCL (BV-MCL...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/UD2C-6JVP-WHXQ-Q217
更新日期:2004-07-01 00:00:00
abstract::A 65-year-old man with lower back pain was found to have multiple myeloma. Unique findings in the marrow aspirate smears of this patient were tadpolelike plasma cells and cellular rosettes simulating those seen in neuroblastoma. This case documents yet another facet of the broad morphologic spectrum of neoplastic plas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/98.6.630
更新日期:1992-12-01 00:00:00
abstract::A number of reports have highlighted discrepancies between the DuPont aca and fibrometer-based methods for quantitating plasma fibrinogen levels. Although many authors have suggested that the presence of fibrin(ogen) degradation products are in some way responsible for the discrepancies, no direct test of this hypothe...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.4.490
更新日期:1987-10-01 00:00:00
abstract::Heterogeneous expression or amplification is a challenge to HER2 diagnostics. A guideline defines heterogeneity as the presence of between 5% and 50% cells with HER2/CEP17 ratios of more than 2.20. We audited the frequency of such cells and their clinical impact in the results from 2 pathology laboratories combined wi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP0EN6AQMWETZZ
更新日期:2011-08-01 00:00:00
abstract::We assessed cytologic specimens from 11 mantle cell lymphomas (MCLs) and 32 other B-cell non-Hodgkin lymphomas (NHLs) for 11q13 breakpoints using a 2-color fluorescence in situ hybridization (FISH) assay that uses an 11q13 probe centered on the CCND1 gene and a centromeric chromosome 11 probe (CEP11). The number of nu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/69EJ-RFM5-E976-BUTP
更新日期:2000-08-01 00:00:00
abstract::Adenocarcinomas expressing gastric mucins often are accompanied by ectopic gastric mucosa or pyloric metaplasia. We have previously described mucinous bronchioloalveolar carcinoma (mucinous BAC) producing gastric mucins. In the lung tissue, mucous cells seemingly similar to gastric mucous cells are present in congenit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/110.4.450
更新日期:1998-10-01 00:00:00
abstract:OBJECTIVES:To describe consecutive vulvar biopsy cases and to create an educational template for pathology trainees and practicing pathologists. METHODS:We reviewed 189 consecutive biopsies from the female genital area skin and mucosa. We classified them based on etiologies and examined limited clinical information. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa135
更新日期:2020-09-11 00:00:00
abstract::We describe a method of spectrophotometric detection of BCR/ABL chimeric sequences amplified by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), enabling the use of archival hematologic slides as RNA sources. Multiplex PCR amplified b3a2, b2a2, and e1a2 break points of the BCR/ABL translocation and ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/108.4.383
更新日期:1997-10-01 00:00:00
abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.15
更新日期:1991-07-01 00:00:00
abstract:OBJECTIVES:Special AT-rich binding protein 2 (SATB2) immunohistochemistry (IHC) has high sensitivity and specificity for colorectal adenocarcinoma (CRC), but data on its expression in specific subsets of pulmonary, gastric, small bowel, and pancreatobiliary adenocarcinomas (ADCAs) are relatively limited or discordant. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa118
更新日期:2021-01-04 00:00:00
abstract::Cytopathologic smears and/or imprints of human adrenal cortex (9 cases) and its disorders were examined, including adrenocortical nodule (3 cases), adrenocortical adenoma (23 cases), carcinoma (8 cases), and renal cell carcinoma (6 cases). Immunocytochemistry directed against 3 beta-hydroxysteroid dehydrogenase and ad...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.2.161
更新日期:1995-08-01 00:00:00
abstract::The p63 gene, a member of the p53 family, is an epithelial marker expressed in embryonic ectoderm, breast myoepithelium, prostate, oral epithelium, epidermis, and urothelium. The DeltaN-p63 isoforms of p63, which are believed to behave as oncogenes, are expressed in squamous cell carcinoma, basal cell carcinoma, and t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/2GAYKPDDM85P2VEW
更新日期:2007-03-01 00:00:00
abstract::Increasingly, all automated blood counts are not accompanied by a microscopic white blood cell differential. A popular strategy is to obtain a manual differential if any part of the automated blood count and differential is outside specified limits (the "diff-if" strategy). The authors compared two sets of criteria to...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/90.2.163
更新日期:1988-08-01 00:00:00
abstract::A patient with prolymphocytic leukemia was immunophenotyped by whole blood and Ficoll-Hypaque methods. Discordance was noted between the two methods, especially for the HLE1, B1, I2, and T1B monoclonal markers. The disparity in results reflected the use of 15 X 10(6) cells versus 1 X 10(6) cells per reaction tube. Thi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.2.227
更新日期:1989-02-01 00:00:00
abstract::Seven monoclonal antibodies of rat origin were generated against human epidermal keratins and were assayed by immunoblot and by immunoperoxidase staining of frozen sections from various human epithelial tissues. By immunoblotting, the antibodies recognized two different subsets of high molecular weight cytokeratin pol...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/89.3.315
更新日期:1988-03-01 00:00:00