Abstract:
:The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples and 17 primary antibodies were used. The sensitivity of immunocytochemical studies in alcohol-fixed specimens was similar and correlated to that of formalin-fixed specimens, suggesting that alcohol-fixed cytocentrifuged preparations are useful in effusion cytology. Pretreatment with or without heat-induced antigen retrieval revealed that antigen retrieval was unnecessary for immunocytochemical studies with most primary antibodies in alcohol-fixed cytocentrifuged preparations. The present study describes the use of immunocytochemical studies with alcohol-fixed cytocentrifuged preparations for diagnosis in routine effusion cytology.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Ikeda K,Tate G,Suzuki T,Mitsuya Tdoi
10.1309/AJCPAH5WR2LFTUSIsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
934-42issue
6eissn
0002-9173issn
1943-7722pii
136/6/934journal_volume
136pub_type
杂志文章abstract::Pseudomonas alcaligenes is a common soil and water inhabitant that has rarely been proven a human pathogen. We describe a fatal case of Pseudomonas alcaligenes endocarditis. The need for accurate identification of unusual organisms isolated in a clinical setting are discussed. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.2.245
更新日期:1983-02-01 00:00:00
abstract::We evaluated the immunohistochemical profile and specificity of CD138 reactivity in 238 specimens from hematopoietic and nonhematopoietic neoplasms. In 91 bone marrow biopsies, CD138 reactivity was observed for nonneoplastic plasma cells, neoplastic plasma cells in multiple myeloma cases (43/43), and the plasmacytic c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
abstract::A method combining alizarin red and a modified silver stain for reticulum fibers in tissue sections is described. The method allows precise localization of calcium deposits and crystals in relation to epithelial and interstitial connective tissue elements. The deposits and their spatial relationships are clearly disce...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1981-03-01 00:00:00
abstract::Clq precipitins were assayed in a model system by absorption nephelometry. Polyethylene glycol was used to extract immune complexes so that sufficient sensitivity with minimal interference could be achieved. Using a double beam spectrophotometer, we found that the reaction of immune complexes with Clq occurred rapidly...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
abstract::Upon hospital admission a patient was found to have severe anemia and a strongly positive direct antiglobulin test (DAT). The patient was taking probenecid periodically for gout. An antibody was detected in the patient's serum that only reacted with red blood cells (RBCs) when probenecid was added. Eluates from the pa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.3.391
更新日期:1985-09-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is one of the most potent mitogenic, highly specific tumor angiogenic factors, which acts via binding to 2 specific tyrosine kinase receptors. There are few studies analyzing VEGF receptor expression in prostate cancer cells, and results are contradictory. In an immunohistoche...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2001-07-01 00:00:00
abstract::To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Immunocytochemical assays for progesterone receptor (PR) using monoclonal antirabbit PR antibodies (PR-ICA) and for estrogen receptor (ER) (ER-ICA) were compared with radioligand binding (dextran-coated charcoal [DCC]) methods for receptor determination in patients with breast cancer. Immunocytochemical staining for P...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::We compared the anti-estrogen receptors (ER) SP1, 6F11, and 1D5 antibodies in breast carcinoma cases with different ranges of positive cells to evaluate whether this could generate different therapies for patients. We selected 66 cases of breast cancer, each of which was immunostained with the 3 antibodies. 1D5 was le...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPLX0QJROV2IJG
更新日期:2012-11-01 00:00:00
abstract::The immunohistologic distribution of the feto-acinar pancreatic protein (FAP), detected by the monoclonal antibody (MoAb) J28 using an indirect immunoperoxidase technique, is described. Tests were carried out on normal adult pancreas (n = 10), chronic pancreatitis (n = 14), pancreatic adenocarcinoma (n = 17), intraabd...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/93.1.14
更新日期:1990-01-01 00:00:00
abstract::In patients infected with HIV, high-grade B-cell non-Hodgkin's lymphomas (NHL), including the small noncleaved cell (SNCC) category, exhibit pleomorphic features, which makes precise definition difficult. Sixty-nine pathologic specimens with HIV-related systemic lymphomas, including 42 SNCC, 20 immunoblastic lymphomas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.5.561
更新日期:1995-05-01 00:00:00
abstract::The prehospital environment has not been studied for point-of-care testing. Therefore, the authors' helicopter program evaluated the performance of the i-STAT Portable Clinical Analyzer, a rapid point-of-care, hand-held instrument. The primary aim of the study was to determine if the i-STAT Portable Clinical Analyzer ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::A test for the detection of antibodies to HTLV-III is available and will be widely used to screen donated blood to prevent transfusion-associated acquired immunodeficiency syndrome (AIDS). Based upon the sensitivity and specificity, the authors calculated the expected predictive values for different groups of asymptom...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.6.700
更新日期:1986-06-01 00:00:00
abstract::The overall performances of several enzyme reagent kits for alkaline phosphatase, creatine kinase, lactic dehydrogenase, and aspartate aminotransferase were evaluated using an ABA-100 Bichromatic Analyzer. Interassay precision using this instrument with commercial reagents compared well with published data for similar...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/72.2.151
更新日期:1979-08-01 00:00:00
abstract::The development of a precise, mathematical relationship between blood pressure and renal microvascular abnormalities would be highly desirable. Such a relationship would require that abnormalities be quantitative on a rational scale. The dominant abnormality in nephrosclerosis occurs in arcuate and cortical arteries o...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.3.312
更新日期:1986-03-01 00:00:00
abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.15
更新日期:1991-07-01 00:00:00
abstract::Although red blood cell (RBC) antigen typing by agglutination is generally useful, several situations exist where this approach is difficult or impossible. For example, following a massive transfusion, a patient's residual RBCs are mixed with transfused normal donor RBCs. In this case, typing by hemagglutination prima...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.3.353
更新日期:1995-03-01 00:00:00
abstract::The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.3.343
更新日期:1978-09-01 00:00:00
abstract::This study evaluates the sensitivity and detection time of a new radiometric broth (BACTEC 13A medium, Johnston Laboratories, Towson, MD) designed for direct blood culturing for Mycobacteria. A total of 1,848 blood specimens were cultured in parallel by a new method in which blood was directly inoculated into 13A medi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.3.316
更新日期:1989-03-01 00:00:00
abstract::The authors describe the case of a 33-year-old man with a solitary cystic lesion of the left distal radius with central radiodensities that radiographically suggested a chondroid matrix. Curettage of the lesion revealed numerous calcospherites with a lamellar structure. This lamellar pattern is thought to be a result ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/92.6.831
更新日期:1989-12-01 00:00:00
abstract::Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP m...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.2.237
更新日期:1984-02-01 00:00:00
abstract::An American black family in whom hemoglobin J Baltimore and beta (+)-thalassemia genes coexisted is described. The proposita is a 23-year-old woman with a hemoglobin (Hb) level of 11.5 g/dl, microcytic, hypochromic indices, increased values of Hbs A2 and F, and alpha/non-alpha synthetic ratio of 1.52. Hbs A and J Balt...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.6.843
更新日期:1981-06-01 00:00:00
abstract::We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract:Objectives:To determine the impact of systemwide charge display on laboratory utilization. Methods:This was a randomized controlled trial with a baseline period and an intervention period. Tests were randomized to a control arm or an active arm. The maximum allowable Medicare reimbursement rate was displayed for tests...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,随机对照试验
doi:10.1093/ajcp/aqx110
更新日期:2017-11-20 00:00:00
abstract::Peripheral blood mononuclear cells from pemphigus vulgaris and bullous pemphigoid patients produced antiepithelial antibodies in a pokeweed mitogen-stimulated in vitro cell culture system. Antiintercellular substance (ICS) antibodies were detected in supernatants of cell cultures from four of four pemphigus vulgaris p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.5.586
更新日期:1984-05-01 00:00:00
abstract:Objectives:Hemolyzed blood samples commonly occur in hospital emergency departments (EDs). Our objective was to determine whether replacing standard large-volume/high-vacuum sample tubes with low-volume/low-vacuum tubes would significantly affect ED hemolysis. Methods:This was a prospective intervention of the use of ...
journal_title:American journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1093/ajcp/aqx082
更新日期:2017-10-01 00:00:00
abstract::The evaluation of quality control (QC) practices from the College of American Pathologists (CAP) Microbiology Surveys demonstrates a trend away from daily controls similar to that first noted in 1980. Previous recommendations for more relevant cost-containment oriented weekly QC procedures are discussed, and the 1982 ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1983-10-01 00:00:00
abstract::Basaloid squamous cell carcinoma (BSCC) and carcinosarcoma of the esophagus are rare entities, making up fewer than 2% of esophageal malignancies. Comparative genomic hybridization (CGH) in 1 case of BSCC and 2 cases of carcinosarcoma and subsequent array CGH in 1 case each of BSCC and carcinosarcoma revealed common c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPZ1O7UUUISPNR
更新日期:2011-04-01 00:00:00
abstract::The role of viruses in several renal diseases is not documented clearly. The authors attempted to localize envelope glycoproteins of Hantaan virus in biopsy specimens from patients with Korean hemorrhagic fever (KHF) as evidence of direct viral invasion of renal tissues. The authors studied sequential sections of kidn...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/100.4.398
更新日期:1993-10-01 00:00:00
abstract:OBJECTIVES:To assess the ability of flow cytometry (FC) to detect putative neoplastic T-cell subsets on skin shave biopsy (SSB) specimens from patients with mycosis fungoides (MF) and to study the immunophenotype of skin-infiltrating tumor cells in MF. METHODS:SSB specimens from patients with suspected MF were bisecte...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPWE2HBFCGDIDS
更新日期:2015-06-01 00:00:00