Pemphigus and pemphigoid antibody production by pokeweed mitogen-stimulated peripheral blood mononuclear cell cultures in vitro.

Abstract:

:Peripheral blood mononuclear cells from pemphigus vulgaris and bullous pemphigoid patients produced antiepithelial antibodies in a pokeweed mitogen-stimulated in vitro cell culture system. Antiintercellular substance (ICS) antibodies were detected in supernatants of cell cultures from four of four pemphigus vulgaris patients by indirect immunofluorescence. Similarly, antibasement membrane (BM) antibodies were detected in supernatants of cell cultures from three of four bullous pemphigoid patients. No anti-ICS or anti-BM antibodies were detected in supernatants of cell cultures of six normal controls, although antinuclear antibodies were detected occasionally. This pokeweed mitogen-stimulated in vitro cell culture system is suggested as a model to study the role of defects in immunoregulation in the pathogenesis of pemphigus vulgaris and bullous pemphigoid.

journal_name

Am J Clin Pathol

authors

Fitzmaurice M,Deodhar SD

doi

10.1093/ajcp/81.5.586

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

586-90

issue

5

eissn

0002-9173

issn

1943-7722

journal_volume

81

pub_type

杂志文章
  • CDX-2 expression is a common event in primary intestinal-type endocervical adenocarcinoma.

    abstract::We studied the expression of cytokeratin (CK) 7, CK20, CDX-2, and p16 in 119 cervical adenocarcinomas (65 usual type [50 invasive; 15 in situ], 37 intestinal type [21 invasive; 16 in situ], 10 endometrioid, 5 adenosquamous, and 2 signet-ring carcinomas) in comparison with 55 cases of rectal adenocarcinomas. The percen...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP7E5ASGOENPFP

    authors: Saad RS,Ismiil N,Dubé V,Nofech-Mozes S,Khalifa MA

    更新日期:2009-10-01 00:00:00

  • Peliosis of the spleen.

    abstract::Peliosis of the spleen was found in 10 out of 1,200 cases autopsied from 1977 through 1980 in our laboratory. Eight such cases had no peliosis of the liver. Histologically, it was noticed that the parafollicular areas were the most common sites of the lesions, and this feature seemed to be important for the histologic...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.6.708

    authors: Tada T,Wakabayashi T,Kishimoto H

    更新日期:1983-06-01 00:00:00

  • Sensitivity of HER-2/neu antibodies in archival tissue samples of invasive breast carcinomas. Correlation with oncogene amplification in 160 cases.

    abstract::Overexpression and amplification of the HER-2 oncogene in patients with breast cancer has correlated with early onset of metastasis, resistance to hormonal therapy and some forms of chemotherapy, and shortened survival. Therefore, evaluation of this putative prognostic or predictive factor seems critical. Because diff...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/0F58-0GRX-FK4R-A6VA

    authors: Gancberg D,Lespagnard L,Rouas G,Paesmans M,Piccart M,Di Leo A,Nogaret JM,Hertens D,Verhest A,Larsimont D

    更新日期:2000-05-01 00:00:00

  • A civilian case of fatal meningococcemia due to Neisseria meningitidis, group Y.

    abstract::The present report is that of a civilian episode of fatal, fulminant group Y meningococcemia in a previously healthy adolescent, who denied prior vaccination against group C meningococcus. The patient suffered abrupt onset of purpura, hypotension and cardiopulmonary arrest. A detailed clinical and pathologic report is...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/68.2.296

    authors: Bernad PG,Snyder DA,Levey M

    更新日期:1977-08-01 00:00:00

  • Workload recording by microcomputer.

    abstract::Workload recording as designed by the College of American Pathologists has proven to be an invaluable aid in the assessment of overall laboratory operation. The administrative information derived from the compilation of these workload statistics is utilized to provide effective and these workload statistics is utilize...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.4.469

    authors: Norbut AM,Foulis PR,Mendelow H

    更新日期:1980-10-01 00:00:00

  • Preventing Genetic Testing Order Errors With a Laboratory Utilization Management Program.

    abstract:OBJECTIVES:To characterize error rates for genetic test orders between medical specialties and in different settings by examining detailed order information. METHODS:We performed a retrospective analysis of a detailed utilization management case database, comprising 2.5 years of data and almost 1,400 genetic test orde...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqw105

    authors: Mathias PC,Conta JH,Konnick EQ,Sternen DL,Stasi SM,Cole BL,Astion ML,Dickerson JA

    更新日期:2016-08-01 00:00:00

  • Influence of blood collection systems on the prothrombin time and international sensitivity index determined with human and rabbit thromboplastin reagents.

    abstract::Three brands of blood collection tubes were studied for their influence on the prothrombin time (PT) and international sensitivity index (ISI) for 5 commercial thromboplastin reagents. With all reagents, PTs were shorter in Vacutainer (Becton Dickinson Vacutainer Systems, Plymouth, England) samples than in S-Monovette...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/NW194EAMD4WMEHJD

    authors: van den Besselaar AM,Hoekstra MM,Witteveen E,Didden JH,van der Meer FJ

    更新日期:2007-05-01 00:00:00

  • Postchemotherapy histiocyte-rich pseudotumor involving the spleen.

    abstract::We report 2 cases of splenic postchemotherapy histiocyte-rich pseudotumor. Each patient had a history of diffuse large B-cell lymphoma, treated with multiagent chemotherapy. Computed tomography scans performed on both patients showed splenic masses. A positron emission tomography scan performed on 1 patient showed inc...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPC67QOGJXCEZB

    authors: Chandra P,Wen YH,Tuli S,Raphael BG,Amorosi EL,Medeiros LJ,Ibrahim S

    更新日期:2009-09-01 00:00:00

  • Bone marrow involvement by marginal zone B-cell lymphomas of different types.

    abstract::We compared the morphologic findings of different types of marginal zone B-cell lymphoma (MZL) involving the bone marrow (BM), including 18 splenic (SMZL), 6 extranodal (mucosa-associated lymphoid tissue lymphoma), and 6 nodal cases. The median percentage of BM involvement was 15%, and multiple overlapping patterns of...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/HRHQFBFTR8B4LXT4

    authors: Inamdar KV,Medeiros LJ,Jorgensen JL,Amin HM,Schlette EJ

    更新日期:2008-05-01 00:00:00

  • Computer-assisted diagnostic surgical pathology reporting.

    abstract::A very simple method of computer-assisted reporting of surgical pathology (including gross description, microscopic description, and diagnosis) has been developed. This method is based on a modification of the existing microbiology program and files in the comprehensive MedLab Company Pathlab computer system, with the...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.3.337

    authors: Davey PW,Vaughan KD

    更新日期:1982-09-01 00:00:00

  • Pseudo-Pelger-Huët anomaly induced by medications: a clinicopathologic study in comparison with myelodysplastic syndrome-related pseudo-Pelger-Huët anomaly.

    abstract::Pseudo-Pelger-Huët anomaly (PPHA) has been documented in association with transplant medications and other drugs. This iatrogenic neutrophilic dysplasia is reversible with cessation or adjustment of medications but is frequently confused with myelodysplastic syndrome (MDS) based on the conventional concept that PPHA i...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPVFY95MAOBKRS

    authors: Wang E,Boswell E,Siddiqi I,Lu CM,Sebastian S,Rehder C,Huang Q

    更新日期:2011-02-01 00:00:00

  • Hidden mastocytosis in acute myeloid leukemia with t(8;21)(q22;q22).

    abstract:OBJECTIVES:To assess the frequency of systemic mastocytosis (SM) in a large series of acute myeloid leukemia (AML) with t(8;21)(q22;q22). METHODS:We retrospectively characterized 40 bone marrow aspirate smears and biopsy specimens from patients with AML with t(8;21) for the presence of SM. Cases were assessed for mast...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP1Q0YSXEAHNKK

    authors: Johnson RC,Savage NM,Chiang T,Gotlib JR,Cherry AM,Arber DA,George TI

    更新日期:2013-10-01 00:00:00

  • Immune hemolytic anemia associated with probenecid.

    abstract::Upon hospital admission a patient was found to have severe anemia and a strongly positive direct antiglobulin test (DAT). The patient was taking probenecid periodically for gout. An antibody was detected in the patient's serum that only reacted with red blood cells (RBCs) when probenecid was added. Eluates from the pa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.3.391

    authors: Sosler SD,Behzad O,Garratty G,Lee CL,Postoway N,Khomo O

    更新日期:1985-09-01 00:00:00

  • Detection of BRAF mutations in thyroid nodules by allele-specific PCR using a dual priming oligonucleotide system.

    abstract::With various methods, BRAF mutations have been detected in 73.4% to 87.1% of papillary thyroid carcinomas (PTCs) in Korea. We assessed the ability of allele-specific polymerase chain reaction using a dual priming oligonucleotide system, compared with direct sequencing and pyrosequencing, to detect BRAF mutations in fi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPO3F2ENKMDTUS

    authors: Lee HJ,Choi J,Hwang TS,Shong YK,Hong SJ,Gong G

    更新日期:2010-05-01 00:00:00

  • Consultative (expert) second opinions in soft tissue pathology. Analysis of problem-prone diagnostic situations.

    abstract::We reviewed 500 consecutive soft tissue lesions referred for expert consultation to determine types of lesions and/or situations in which major discrepancies occur. Of 266 cases (53.2%) accompanied by a diagnosis, essential agreement with the second opinion was noted in 68%, minor discrepancy in 7%, and major discrepa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/425H-NW4W-XC9A-005H

    authors: Arbiser ZK,Folpe AL,Weiss SW

    更新日期:2001-10-01 00:00:00

  • bcl-2 expression by multicolor flow cytometric analysis assists in the diagnosis of follicular lymphoma in lymph node and bone marrow.

    abstract::The expression of bcl-2, CD10, and CD20 was examined by multicolor flow cytometry in 78 samples including lymph node or other tissue biopsy specimens containing follicular lymphoma (FL; n = 17), reactive hyperplasia (RH; n = 28), or other malignant lymphomas (n = 20), as well as bone marrow aspirates (n = 13). The pre...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/820R-476K-Y9G0-3L9A

    authors: Cook JR,Craig FE,Swerdlow SH

    更新日期:2003-01-01 00:00:00

  • Cytodifferentiated renal tumors occurring with Wilms' tumors of the opposite kidneys: report of two cases.

    abstract::The most common differentiated renal tumor of early childhood is the congenital mesoblastic neophroma. Well-differentiated forms of Wilms' tumor may present a difficult differential diagnosis from these benign neoplasms. The cases of two patients found to have well-differentiated renal neoplasms after being treated fo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/66.2.376

    authors: Hughson MD,Hennigar GR,Othersen HB Jr

    更新日期:1976-08-01 00:00:00

  • Clinical impact of point-of-care vs laboratory measurement of anticoagulation.

    abstract::Patients using anticoagulation point-of-care (POC) monitors are advised to periodically test these systems against laboratory methods to monitor performance. The international normalized ratio (INR), however, can vary between test systems owing to different instrument-reagent combinations. In a randomized study evalua...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Sunderji R,Gin K,Shalansky K,Carter C,Chambers K,Davies C,Schwartz L,Fung A

    更新日期:2005-02-01 00:00:00

  • Nephrogenic metaplasia of the ureter.

    abstract::We present what, to our knowledge, is the first reported case of nephrogenic metaplasia of the ureter. Nephrogenic metaplasia involves the transitional epithelium of the urinary tract and results in the formation of epithelial tubules, which are histologically similar to renal tubules. Special stains demonstrated both...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/80.1.92

    authors: Lugo M,Petersen RO,Elfenbein IB,Stein BS,Duker NJ

    更新日期:1983-07-01 00:00:00

  • Tumor-Infiltrating Lymphocytes in Breast Cancer: Evaluating Interobserver Variability, Heterogeneity, and Fidelity of Scoring Core Biopsies.

    abstract:Objectives:The aims were to evaluate breast cancer (BC) tumor-infiltrating lymphocytes (TILs) interobserver variability, heterogeneity, and the fidelity of scoring TILs in core needle biopsy (CNB). Methods:Matching CNB and two full-face sections (FFSs) of BC cases (n = 100) were independently reviewed by two pathologi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqy069

    authors: Khoury T,Peng X,Yan L,Wang D,Nagrale V

    更新日期:2018-10-01 00:00:00

  • Evaluation of a commercial exoantigen test system for the rapid identification of systemic fungal pathogens.

    abstract::Seventy-nine mycelial-form stock cultures of Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, and morphologically similar fungi were extracted and tested by using commercial macroimmunodiffusion exoantigen test kits and the Centers for Disease Control (CDC) reference system for identifying funga...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.3.379

    authors: Denys GA,Newman MA,Standard PG

    更新日期:1983-03-01 00:00:00

  • Quality assurance measures for critical diagnoses in anatomic pathology.

    abstract::We sought to characterize how well computerized "case flags" performed in evaluating critical diagnoses in anatomic pathology. All cases identified by a pathologist at sign-out and flagged as a critical diagnosis in anatomic pathology in 2 hospital laboratories during a 3-year period were reviewed. A subset of all cri...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPL1N5KBAFFDHV

    authors: Renshaw A,Gould EW

    更新日期:2012-03-01 00:00:00

  • Phenotypic and genotypic characterization of carcinomas of the papilla of Vater has prognostic and putative therapeutic implications.

    abstract::We further characterize the heterogeneous carcinomas of the papilla of Vater (CPVs) in relation to various clinicopathologic patient characteristics and patient survival. Of the 71 reevaluated CPVs, 32 were intestinal, 26 were pancreatobiliary, 6 were mixed, 4 were mucinous, and 3 were poorly differentiated carcinomas...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPCTCUQSYI89YT

    authors: Kohler I,Jacob D,Budzies J,Lehmann A,Weichert W,Schulz S,Neuhaus P,Röcken C

    更新日期:2011-02-01 00:00:00

  • High percentage of JAK2 exon 12 mutation in Asian patients with polycythemia vera.

    abstract::We examined the occurrence of JAK2(V617F) and JAK2 exon 12 mutations in a clinical cohort of polycythemia vera (PV) in Taiwan. Of 22 patients with PV, 17 (77%) had the V617F mutation, and all 5 V617F-negative patients (23%) had the exon 12 mutation. We found 3 different exon 12 mutations: 3 N542-E543del, 1 F537-K539de...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPK7KGOWPHYWM0

    authors: Yeh YM,Chen YL,Cheng HY,Su WC,Chow NH,Chen TY,Ho CL

    更新日期:2010-08-01 00:00:00

  • The New Equivocal: Changes to HER2 FISH Results When Applying the 2013 ASCO/CAP Guidelines.

    abstract:OBJECTIVES:Human epidermal growth factor receptor 2 (HER2, ERBB2) testing is an important prognostic/predictive marker in breast cancer management, especially in selecting HER2-targeted treatment. American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines address HER2 status and were...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP3Q9WFOQTKUVV

    authors: Long TH,Lawce H,Durum C,Moore SR,Olson SB,Gatter K,Troxell ML

    更新日期:2015-08-01 00:00:00

  • The effect of the platelet count on the aggregation response and adenosine triphosphate release in an impedance lumi-aggregometer.

    abstract::Platelet aggregation studies are usually performed in either electrical impedance or optical systems and the release reaction assessed by quantitating adenosine triphosphate (ATP) from luminesence produced by the firefly luciferin-luciferase system. In evaluating the results of such studies, attention is paid to a var...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.5.655

    authors: Sweeney JD,Labuzetta JW,Fitzpatrick JE

    更新日期:1988-05-01 00:00:00

  • Elastosis and elastofibromatous change in the gastrointestinal tract: a clinicopathologic study of 13 cases and a review of the literature.

    abstract::We describe 13 cases in which the submucosa and muscularis mucosae of the gastrointestinal tract exhibited a focal or diffuse increase of elastin fibers. This elastosis or elastofibromatous change most commonly manifested as a colonic polyp and usually was found during screening colonoscopy. Gastric and small intestin...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/LBGT-CG1Q-CD96-M3NF

    authors: Hobbs CM,Burch DM,Sobin LH

    更新日期:2004-08-01 00:00:00

  • Effectiveness of multiple initiatives to reduce blood component wastage.

    abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP42WMHSSTPHXI

    authors: Collins RA,Wisniewski MK,Waters JH,Triulzi DJ,Yazer MH

    更新日期:2015-03-01 00:00:00

  • Immunohistochemical staining for terminal deoxynucleotidyl transferase (TDT). An enhanced method in routinely processed formalin-fixed tissue sections.

    abstract::This report describes an improved technique for sensitive and specific localization of terminal deoxynucleotidyl transferase (TdT) in routinely processed paraffin-embedded, formalin-fixed tissue sections using DNAse pretreatment and the avidin-biotin complex (ABC) technique. This method is useful in identifying lympho...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.5.649

    authors: Said JW,Shintaku IP,Pinkus GS

    更新日期:1988-05-01 00:00:00

  • Predictive value of a screening test for antibodies to HTLV-III.

    abstract::A test for the detection of antibodies to HTLV-III is available and will be widely used to screen donated blood to prevent transfusion-associated acquired immunodeficiency syndrome (AIDS). Based upon the sensitivity and specificity, the authors calculated the expected predictive values for different groups of asymptom...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/85.6.700

    authors: Sivak SL,Wormser GP

    更新日期:1986-06-01 00:00:00