Abstract:
:This report describes an improved technique for sensitive and specific localization of terminal deoxynucleotidyl transferase (TdT) in routinely processed paraffin-embedded, formalin-fixed tissue sections using DNAse pretreatment and the avidin-biotin complex (ABC) technique. This method is useful in identifying lymphoblastic lymphomas (14/15 cases positive), with all other B- and T-cell lymphomas tested negative for the reaction. Used in conjunction with monoclonal antibodies immunoreactive for T- and B-cells in paraffin sections this technique should prove helpful in immunophenotyping malignant lymphomas where fresh tissue is unavailable for study.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Said JW,Shintaku IP,Pinkus GSdoi
10.1093/ajcp/89.5.649subject
Has Abstractpub_date
1988-05-01 00:00:00pages
649-52issue
5eissn
0002-9173issn
1943-7722journal_volume
89pub_type
杂志文章abstract::Epithelial hyperplasia, a component of proliferative breast disease (PBD), is a known risk factor for the development of breast carcinoma. To determine if criteria established for tissue biopsy could be used to more precisely define cytologic criteria for diagnosis of PBD, 37 breast fine-needle aspirates with biopsy d...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.4.438
更新日期:1995-04-01 00:00:00
abstract::An anti-serum against human neuron-specific enolase (NSE), raised in sheep, has been characterized and used for immunocytochemical localization of NSE in paraffin sections of normal tissues and lung cancers. Of the small cell carcinomas (SCC), 69 out of 99 (70%) cases stained with the anti-serum. Maltreated biopsies s...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.1.1
更新日期:1985-07-01 00:00:00
abstract::Abbreviated pretransfusion testing, although permitted by American Association of Blood Banks Standards for unimmunized patients, is not widely practiced. Concerns remain about optimal antibody screening methods, antibodies missed by deleting the antiglobulin crossmatch, and cost-effectiveness. The authors prospective...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.4.428
更新日期:1990-10-01 00:00:00
abstract::Statin is a 57 kDa nuclear protein exclusively found in noncycling cells. Its expression in hematolymphoid cells had not been examined previously. The authors studied statin immunoreactivity in nonneoplastic lymphoid tissues from 26 lymph nodes, 3 tonsils, and 2 spleens. Statin was detected primarily in small lymphoid...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/101.4.421
更新日期:1994-04-01 00:00:00
abstract::Loss of intercellular adhesion facilitates tumor invasion. To clarify the relation between altered expression of cell adhesion molecules and progression of T1 superficial bladder tumors, 101 cases (71 T1 tumors, 30 T2/T3 tumors) were examined immunohistochemically for E-cadherin and alpha-, beta-, and gamma-catenins. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::In this study, I have estimated the percentage of tumor by visual estimate in 447 prostatectomy specimens from American Veterans Affairs patients and related this measurement to overall survival. Although percentage of tumor was significantly related to the serum prostate-specific antigen level, tumor stage, and Gleas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPX5MAMNMFE6FQ
更新日期:2009-01-01 00:00:00
abstract::Acute myeloid leukemia (AML) with isolated trisomy 4 is rare. Associations with KIT mutations on chromosome 4q12 have been documented. The clinicopathologic features and mutational status of KIT, FLT3, NPM1, CEBPA, and RAS were assessed in 13 AML cases with isolated trisomy 4. There were 9 men and 4 women with a media...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP7ZC9YQERSKGX
更新日期:2012-03-01 00:00:00
abstract::The Sysmex R-3000 (TOA Medical Electronics, Kobe, Japan) evaluates maturation of reticulocytes by quantitating the fraction of reticulocytes within low-, middle-, and high-fluorescence intensity regions. We defined the immature reticulocyte fraction (IRF) as the sum of the fraction of high-fluorescence intensity regio...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Current methods for testing stool samples for hemoglobin utilize peroxidase oxidation of chemical indicators such as guaiac or benzidine. These tests have frequent false-positive and false-negative results, complicating random screening for occult gastrointestinal bleeding. The authors have developed an immunochemical...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/69.1.342
更新日期:1978-03-01 00:00:00
abstract::To evaluate the diagnostic utility of flow cytometry (FCM) as compared to cytology, DNA and RNA content of cells were measured in 233 samples of cerebrospinal fluid (CSF) from 147 patients with solid tumors or lymphomas and 17 controls with nonmalignant disorders. All control specimens were normal. Twenty-eight sample...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.5.570
更新日期:1987-11-01 00:00:00
abstract::Remarkable improvements have been made in blood safety since the onset of the HIV epidemic. However, the current paradigm does not prevent all transfusion-transmitted infections and is reactive to new agents, thus accepting that some patients may be harmed before preventive measures are introduced. Several methods are...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/RAPQ3NXG3MV9AL94
更新日期:2007-12-01 00:00:00
abstract::This study describes results of fresh-frozen immunohistochemistry applied to two typical Warthin's tumors. Polyclonal follicular center cell staining and an apparent reduction in the number of T-lymphocytes were found. These observations tend to support the concept that the lymphoid infiltrate in Warthin's tumor repre...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.6.795
更新日期:1980-12-01 00:00:00
abstract:OBJECTIVES:To determine the impact of implementing p16 Lower Anogenital Squamous Terminology Standardization Project for HPV-Associated Lesions (LAST) guidelines, we compared p16 use and follow-up data before and after implementation of the guidelines. METHODS:We reviewed all cervical biopsy specimens diagnosed by two...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPUXLP7XD8OQYY
更新日期:2015-12-01 00:00:00
abstract::The present immunoperoxidase study attempted to localize aldosterone and cortisol in formalin-fixed paraffin-embedded adrenal glands or tumors. Apparently, steroid substances are solubilized during routine tissue processing, and the cytoplasm of adrenal cortical cells is not stained by either antialdosterone or antico...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.1.92
更新日期:1981-01-01 00:00:00
abstract::Descriptive reports of liver histologic features in celiac disease (CD) are sparse, and the effect of a gluten-free diet (GFD) on the course of liver injury is poorly understood. We reviewed liver biopsy specimens in 30 patients with CD and performed immunostains for IgG, IgG4, IgM, and IgA. Subsequent liver biochemic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPDOMY5RI5TPMN
更新日期:2011-07-01 00:00:00
abstract::We studied methylation of 2 tumor suppressor genes (p14, p16) and 4 MINT (methylated in tumor) clones (MINT1, MINT2, MINT25, MINT31) among 51 fundic gland polyps (FGPs) and 27 normal gastric body biopsy samples using bisulfite treatment of genomic DNA followed by methylation-specific polymerase chain reaction. Thirty-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4QUN-J4F2-7QK7-RR0G
更新日期:2004-11-01 00:00:00
abstract::Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.4.371
更新日期:1980-10-01 00:00:00
abstract::A patient with an acute burn was noticed to have spuriously elevated platelet counts on an automated system. The major factor accounting for this discrepancy was the presence of numerous microspherocytes which were in the same size range of platelets. It is thus important to add microspherocytosis as an additional cau...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.2.220
更新日期:1982-02-01 00:00:00
abstract::Coagulase-negative staphylococci are the most common cause of late-onset septicemia in neonates in intensive care nurseries. Clinical and laboratory diagnosis of infection with coagulase-negative staphylococci can be difficult. The authors reviewed serial mean platelet volumes of 18 infants in whom coagulase-negative ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/99.1.69
更新日期:1993-01-01 00:00:00
abstract::The levels of thymus-derived lymphocytes (T-cells) in samples of peripheral blood from 47 patients with carcinoma of the breast, 36 patients with benign mammary lesions, and 33 matched normal controls were determined. Results were analyzed with three statistical tests. It was found that the mean percentages of T-lymph...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.4.445
更新日期:1976-04-01 00:00:00
abstract::By studying more than 1,200 patients with cutaneous melanoma and long-term follow-up, I examined the relationship between solar elastosis and age at diagnosis of melanoma, key features of melanoma, and the outcome of overall survival in melanoma. I found that melanomas with elastosis were diagnosed significantly later...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/7MHX96XH3DTY32TQ
更新日期:2007-08-01 00:00:00
abstract:Objectives:To examine the relationship between the recently defined Gleason grade groups and prostate cancer-specific mortality. Methods:If the probability of prostate cancer-specific death is symbolized as P(PSD), the probability of biochemical failure is symbolized as P(BF), and the probability of prostate cancer-sp...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw212
更新日期:2017-03-01 00:00:00
abstract::CD45/side scatter (SS) gating is widely used for isolating blasts by flow cytometry (FC). However, other cells contaminate the "blast gate" (BG); CD45/SS gating is thus imprecise, particularly when there are few blasts. We studied the BG contents in 21 myelodysplastic syndromes (MDSs), 14 myeloproliferative neoplasms ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPN4G1IZPABRLH
更新日期:2012-05-01 00:00:00
abstract::The serum concentrations of alpha-fetoprotein of rats following experimental galactosamine-induced liver-cell necrosis accurately reflect the severity of preceding liver-cell damage as determined by elevation of serum transaminases. There is a very close correlation between the highest SGOT or SGPT serum activity foun...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/66.5.847
更新日期:1976-11-01 00:00:00
abstract::We assessed the Sysmex UF-50 for reproducibility of results and carryover rate by performing between- and within-run precision analyses on 315 urine samples, evaluated the feasibility of using the UF-50 to measure urinary cellular and noncellular components by comparing results from the UF-50 with results of manual ur...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/RT7X-EMGF-G8AV-TGJ8
更新日期:2001-04-01 00:00:00
abstract::We undertook a retrospective analysis of colonic spirochetosis in 14 cases: females, 3; males, 11; children, 4; adults, 10. Two men had HIV infections. All children and both HIV-infected men had abdominal complaints, diarrhea, or both. Most other adults underwent colonoscopy for polyp screening (n = 4) or follow-up of...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/G7U6-BD85-W4G3-WJ0J
更新日期:2003-12-01 00:00:00
abstract:OBJECTIVES:To calculate the incidence of nondiagnostic (ND) colorectal (CR) polyp cases in which deeper tissue sectioning rendered new diagnostic information--particularly adenomas--in 2 laboratories staffed by the same pathologists. METHODS:After initial diagnosis, 100 ND CR polyps from each laboratory were reexamine...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPXU66QZAZNYN
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to determine if the diagnosis of nephrogenic adenoma (NA) can be made on cytologic criteria alone and if pair box gene transcription factor 8 (PAX8) is useful in the diagnosis of NA in daily cytology practice. METHODS:Cytologic features of NA previously described in a literature wer...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw006
更新日期:2016-03-01 00:00:00
abstract:Objectives:To determine the impact of systemwide charge display on laboratory utilization. Methods:This was a randomized controlled trial with a baseline period and an intervention period. Tests were randomized to a control arm or an active arm. The maximum allowable Medicare reimbursement rate was displayed for tests...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,随机对照试验
doi:10.1093/ajcp/aqx110
更新日期:2017-11-20 00:00:00
abstract::Estrogen receptor (ER), progesterone receptor (PR), and c-ERBB2 (HER2/neu) are therapeutically and prognostically important markers in the management of breast carcinoma. They are not always analyzed in distant metastatic and locally recurrent breast cancers. We compared immunohistochemical expression in a series of p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPJ57FLLJRXKPV
更新日期:2010-03-01 00:00:00