Abstract:
:We studied methylation of 2 tumor suppressor genes (p14, p16) and 4 MINT (methylated in tumor) clones (MINT1, MINT2, MINT25, MINT31) among 51 fundic gland polyps (FGPs) and 27 normal gastric body biopsy samples using bisulfite treatment of genomic DNA followed by methylation-specific polymerase chain reaction. Thirty-two FGPs were syndromic polyps from 14 patients with familial adenomatous polyposis (FAP); 19 were sporadic FGPs from 15 patients without FAP. Significantly higher mean methylation indices were found between FGPs and normal gastric mucosa (P = .012). FGPs arising in a background of proton pump inhibitor (PPI) effect had significantly higher mean methylation indices than those that did not (P = .023). Perhaps because sporadic FGPs were more likely to be associated with PPI effect than were FAP-associated FGPs, they also demonstrated higher mean methylation indices than syndromic polyps (P = .024). Among FAP-associated FGPs, there was no statistical difference in methylation indices between polyps that were dysplastic, indefinite for dysplasia, or nondysplastic (P = .87). Epigenetic alterations involving methylation of CpG islands might have a role in the development of some FGPs, particularly those with a PPI effect. They do not account for the presence or absence of a dysplastic phenotype in FGPs.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Abraham SC,Park SJ,Cruz-Correa M,Houlihan PS,Half EE,Lynch PM,Wu TTdoi
10.1309/4QUN-J4F2-7QK7-RR0Gsubject
Has Abstractpub_date
2004-11-01 00:00:00pages
740-6issue
5eissn
0002-9173issn
1943-7722pii
4QUNJ4F27QK7RR0Gjournal_volume
122pub_type
杂志文章abstract::Several authors have suggested that a plausible mechanism for variation in reported mean corpuscular volume (MCV) and hematocrit values for Hematology Survey Specimens may be due to the presence of hyperglycemia in the suspending matrix of the specimens, resulting in cell swelling due to an osmotic matrix effect. The ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1983-10-01 00:00:00
abstract::The performance characteristics of the XE-2100 (Sysmex, Kobe, Japan) automated immature granulocyte (IG) count were studied. The automated IG count was compared with the manual morphology count and with a proposed reference flow cytometric count. The comparison data were analyzed by both least-squares and Passing-Babl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/TVGKD5TVB7W9HHC7
更新日期:2007-09-01 00:00:00
abstract::A patient who had masses in the breasts clinically suggestive of neoplasia had fibrous mastopathy and striking deposition of ochronotic pigment within the soft tissue of the breast. The gross appearance and light microscopic features are illustrated. The histochemical features of ochronotic pigment and the difficulty ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/71.3.349
更新日期:1979-03-01 00:00:00
abstract::Neuroendocrine tumors comprise a family of neoplasms with a wide range of morphologic, functional, and behavioral characteristics. Their diagnosis depends on the recognition of characteristic morphologic features and on the presence of markers indicative of neuroendocrine differentiation. Neuroendocrine tumors can be ...
journal_title:American journal of clinical pathology
pub_type: 历史文章,杂志文章,评审
doi:10.1309/7GR5-L7YW-3G78-LDJ6
更新日期:2001-06-01 00:00:00
abstract:OBJECTIVES:To establish an objective, scientific, evidence-based process for planning statistical quality control (SQC) procedures based on quality required for a test, precision and bias observed for a measurement procedure, probabilities of error detection and false rejection for different control rules and numbers o...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqy158
更新日期:2019-03-01 00:00:00
abstract::The aims of this study were to compare the quality of immunohistochemical assays of estrogen receptor (ER) and progesterone receptor (PR) and to compare the intermethod variability of assays from different manufacturers. immunohistochemical staining was entrusted to the following laboratories in Japan: Kyowa Medex, de...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4D1A04NCDK96WFY7
更新日期:2007-03-01 00:00:00
abstract::Medicare policies have gradually restricted the scope of pathologist services payable under the rules applicable to physician services. Recent changes applying relative value scales has provided an opportunity for national standardization of pathology codes, and has permitted the introduction of clinical pathology int...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::This report describes an improved technique for sensitive and specific localization of terminal deoxynucleotidyl transferase (TdT) in routinely processed paraffin-embedded, formalin-fixed tissue sections using DNAse pretreatment and the avidin-biotin complex (ABC) technique. This method is useful in identifying lympho...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/89.5.649
更新日期:1988-05-01 00:00:00
abstract::Blastomycosis generally results from inhalation of Blastomyces dermatitidis conidia following exposure to contaminated soil in an endemic area. Primary infections commonly involve the lungs, although secondary dissemination to other body sites may occur. We describe 2 cases of osseous blastomycosis in people living ou...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/JEJ0-3N98-C3G8-21DE
更新日期:2002-10-01 00:00:00
abstract::Proliferative lesions, which include collagen, elastic fibers and abnormally oriented muscle fibers, were found to develop in intramyocardial arteries more rapidly with age in cigarette smokers than in nonsmokers. The study attempted to determine whether these lesions reduce perfusion pressures in the subendocardial z...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/68.4.493
更新日期:1977-10-01 00:00:00
abstract:OBJECTIVES:The 2014 Bethesda System (TBS 2014) guidelines for reporting cervical cytology revised the age for reporting benign endometrial cells (BECs) from 40 years or older to age 45 years or older. We evaluated this change and further investigated if extending the reporting age to 50 years or older may be acceptable...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa041
更新日期:2020-08-05 00:00:00
abstract::This is a report of an adenocarcinoma of mesonephric origin studied by light microscopy, electron microscopy, and immunocytochemistry. Unlike previous reports, our lesion was located paravaginally and not in the leaves of the broad ligament or in the cervix. The light microscopic features are similar to those of previ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.4.539
更新日期:1983-10-01 00:00:00
abstract::Cellular and humoral immunity combine to determine the outcome following exposure to hepatitis virus and are implicated in the proposed pathogenetic mechanism for acute and chronic hepatitis. Although antibody to HBsAg is found in virtually all following recovery from hepatitis B, a cell-mediated response to HBsAg can...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1976-05-01 00:00:00
abstract::The concept of re-engineering rests on computer coordination of information. As a management tool, it has been tested and found to be ambiguous. At best it sharpens laboratory objectives, but at worst it fragments skills and threatens the community attitudes needed for teamwork. Experience with its most fruitful compo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1996-04-01 00:00:00
abstract::Determination of urinary homovanillic acid (HVA) is an important analysis to establish the diagnosis and prognosis of neuroblastoma. A method is presented for the determination of this metabolite of dopamine using its reaction with 1-nitroso-2-naphthol and nitrous acid. The method has shown a sensitivity, specificity,...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/87.5.644
更新日期:1987-05-01 00:00:00
abstract::The authors report a case of primary Ki-1 lymphoma of the brain. The patient was a 4 1/2-year-old black girl who presented with a 4- and 5-day history of headaches, nausea, vomiting, neck stiffness, and difficulty in walking. Computed tomography (CT) scan of the brain showed two discrete densities in the left occipita...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.4.496
更新日期:1995-04-01 00:00:00
abstract::To establish the effect of a quality control failure on the performance of the LCx-GC nucleic-acid amplification assay for Neisseria gonorrhoeae (Abbott Laboratories, Abbott Park, IL) in the field, we conducted a retrospective analysis comparing the clinical and analytic performance of the recalled lots with those not...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/1LD8-LU7Y-FFW7-5WQA
更新日期:2005-06-01 00:00:00
abstract::The Leu-8 antigen is found on the surface of many hematologic cells, including many T- and B-lymphocytes. With the use of a frozen-section immunoperoxidase technic, 152 B-cell non-Hodgkin's lymphomas were examined for Leu-8 expression. Of these lymphomas, 53% expressed Leu-8. Subclassification of the lymphomas with th...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.4.486
更新日期:1987-10-01 00:00:00
abstract::We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage cl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/C8T8-YNB4-G3W4-5PRF
更新日期:2003-07-01 00:00:00
abstract::Cell counter-based formulas have been used in the differential diagnosis of microcytic anemia. The measurement of RBC subpopulations is now available on the Sysmex XE 5000 analyzer (Sysmex, Kobe, Japan). We describe the new formulas: % microcytic - % hypochromic; and % microcytic - % hypochromic - red cell distributio...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
abstract::The results of hepatitis B surface antigen (HBs-Ag) testing in a large volunteer blood donor population are described. Counterelectrophoresis and three versions of solid-phase radioimmunoassay technic are compared and evaluated. Initial results suggested that the radioimmunoassay technic are compared and evaluated. In...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
abstract::The thickness of eosinophilic band in collagenous colitis (CC) was assessed by 3 methods: histologic estimates (22 observers), conventional measurements using a calibrated micrometric scale (1 observer), and semiautomatic micrometric measurements (1 observer). By the histologic estimate technique, 7.4% of the results ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/K7MH8UCK3MR7Q77L
更新日期:2008-09-01 00:00:00
abstract::The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sens...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
abstract::Two cases of Actinomyces viscosus infection of the lungs were seen in nonimmunosuppressed patients. One patient had a peripheral actinomycotic lung mass resembling a tumor. Both patients responded to a long course of penicillin therapy. Reports of A. viscosus infections are rare, although the organism colonizes the mo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.1.113
更新日期:1981-01-01 00:00:00
abstract::The professional activities of anatomic and clinical pathologists involve, to a large extent, management issues. To evaluate the management training received by pathology residents, the authors surveyed pathology residency training programs in the United States. Responses were collected from 138 of 187 programs (73.8%...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/101.5.559
更新日期:1994-05-01 00:00:00
abstract::The effect of changes in cytology laboratory costs, including the costs of new technologies, on the cost-effectiveness of cervical cancer prevention has not been studied. Using University of Iowa laboratory detection rates and costs, a decision model determined the cost-effectiveness of the laboratory with and without...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
abstract::A critical limit protocol requiring that all point of care glucose meter readings > 22.2 mmol/L (400 mg/dL) and < 2.2 mmol/L (40 mg/dL) be immediately confirmed by the laboratory was assessed. A total of 193 (2%) of 9,523 glucose meter determinations (63 patients) were > 22.2 or < 2.2 mmol/L. One hundred twenty-two (6...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.3.390
更新日期:1996-09-01 00:00:00
abstract::Interest in the role of mononuclear phagocytes in glomerulonephritis (GN) and in defining markers of renal neoplasms led the authors to study alpha-naphthyl acetate/butyrate esterase (ANAE/ANBE), alkaline phosphatase (AlkP), acid phosphatase (AcP), 5'-nucleotidase (5'N), and ATPase (ATP) activity in paraformaldehyde-f...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00
abstract::Inhibition of anti-Rh29 by erythrocytic stroma in feces was devised as a specific test for fecal occult blood. The sensitivity of this test was equivalent to that of a standard Hemoccult test, namely, 10(8) erythrocytes/g feces. Comparison of results of this test with results of Hemoccult tests of random stool specime...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/71.2.204
更新日期:1979-02-01 00:00:00
abstract::After the March 2004 implementation of American Association of Blood Banks standards regarding platelet bacterial detection, we began quantitative glucose screening of whole blood-derived platelets (WB-P). The glucose level was measured immediately before component release--often storage day 4 or 5--using the Glucomet...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPVN9OT4GRCSKO
更新日期:2009-04-01 00:00:00