Abstract:
:Interest in the role of mononuclear phagocytes in glomerulonephritis (GN) and in defining markers of renal neoplasms led the authors to study alpha-naphthyl acetate/butyrate esterase (ANAE/ANBE), alkaline phosphatase (AlkP), acid phosphatase (AcP), 5'-nucleotidase (5'N), and ATPase (ATP) activity in paraformaldehyde-fixed, plastic-embedded renal tissue from patients with a variety of pathologic conditions. These conditions included GN, renal tumors, and transplant rejection. Enzymatic staining for ANAE, ANBE, AcP, AlkP, and ATP was generally confined to tubules and collecting ducts in normal kidney. Nine of 10 cases of renal carcinoma had weakly to strongly positive reactions with AlkP, AcP, and ANAE; 9 of 10 cases of Wilms' tumor showed weakly positive reactions with AcP and ANAE, particularly in tubular structures. Severely damaged kidney allografts showed surprising retention of normal histochemical features. In all cases 5'N stained both glomerular capillaries and interstitial vasculature; ATPase and AlkP stained interstitial vessels only. Plastic embedding provides superb preservation of both microscopic anatomy and enzymatic activity, which may allow utilization of enzyme histochemistry for diagnostic and research purposes.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Alpers CE,Beckstead JHdoi
10.1093/ajcp/83.5.605subject
Has Abstractpub_date
1985-05-01 00:00:00pages
605-12issue
5eissn
0002-9173issn
1943-7722journal_volume
83pub_type
杂志文章abstract:OBJECTIVES:To calculate the incidence of nondiagnostic (ND) colorectal (CR) polyp cases in which deeper tissue sectioning rendered new diagnostic information--particularly adenomas--in 2 laboratories staffed by the same pathologists. METHODS:After initial diagnosis, 100 ND CR polyps from each laboratory were reexamine...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPXU66QZAZNYN
更新日期:2013-08-01 00:00:00
abstract::Anatomic pathology service is judged by accuracy of diagnosis and rapidity of reporting of that diagnosis to clinicians. Turnaround times have been used in anatomic pathology to show how long it take to produce a tissue report. Rapid tissue diagnosis is important to patients, clinicians, administrators, and anatomic p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract:OBJECTIVES:BRAF V600E mutation is characteristic of hairy cell leukemia (HCL). A V600E mutation-specific antibody, VE1, has been recently developed. We studied the diagnostic utility of this antibody in HCL and compared it with other B-cell neoplasms. METHODS:VE1 activity was assessed using immunohistochemistry in 90 ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPQLQ89VXTVWKN
更新日期:2015-01-01 00:00:00
abstract::The thymus was the site of a true histiocytic lymphoma. Immunohistochemical demonstration of lysozyme and alpha-1-antitrypsin in the tumor cells indicated the histiocytic nature of the tumor. Five fetal and five adult thymus glands were studied for the presence and distribution of true histiocytic cells. Histiocytes w...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.6.734
更新日期:1984-12-01 00:00:00
abstract:Objectives:Fibromatosis of the breast is an uncommon neoplasm with potential for local recurrence. Treatment has traditionally been surgical excision with current trends toward conservative management. Given the option of observation after diagnosis by core needle biopsy (CNB), we sought to evaluate the accuracy of CNB...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqx065
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:In 2001, the University of New Mexico Hospitals implemented a rapid screening tool for the triage of suspected hantavirus cardiopulmonary syndrome based on peripheral blood smear morphology. Five criteria guided clinical decisions: thrombocytopenia, hemoconcentration, granulocytic left shift, absence of toxi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPNFVWG46NUHED
更新日期:2014-08-01 00:00:00
abstract::Serum bicarbonate testing has been reported to be inaccurate in several clinical studies. This prompted a search for systemic errors in bicarbonate testing devices, which has been inconclusive. The effect on serum bicarbonate level measurement of partially filling plain red-top (clot) vacutainer tubes was studied in h...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.2.213
更新日期:1992-02-01 00:00:00
abstract::Platelet factor 4 (PF4)/heparin antibody, typically associated with heparin therapy, is reported in some heparin-naive people. Seroprevalence in the general population, however, remains unclear. We prospectively evaluated PF4/heparin antibody in approximately 4,000 blood bank donors using a commercial enzyme-linked im...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPG0MNR5NGKNFX
更新日期:2010-11-01 00:00:00
abstract::We undertook a retrospective analysis of colonic spirochetosis in 14 cases: females, 3; males, 11; children, 4; adults, 10. Two men had HIV infections. All children and both HIV-infected men had abdominal complaints, diarrhea, or both. Most other adults underwent colonoscopy for polyp screening (n = 4) or follow-up of...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/G7U6-BD85-W4G3-WJ0J
更新日期:2003-12-01 00:00:00
abstract::Mantle cell lymphoma (MCL) is characterized by typical morphologic features and the CD5+, CD23-immunophenotype. However, some morphologically typical MCLs are CD23+. A t(11;14)(q13;q32) translocation is frequently found in MCL, leading to overexpression of cyclin D1. We studied the expression of cyclin D1 in 50 small ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/R2BC-2H7T-7KB6-0YG8
更新日期:2002-02-01 00:00:00
abstract::Recent reports show that the sensitivity of endometrial carcinoma detection on liquid-based Papanicolaou (Pap) tests (88%) is considerably higher than that reported on conventional Pap smears (20%-30%), although few laboratories have corroborated these results. We performed a 5-year retrospective review of all liquid-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPLFBK1A2XJDQI
更新日期:2012-02-01 00:00:00
abstract::Under carefully controlled conditions in a clinical research ward, conjugated primary bile-acid concentrations were measured by a 125I-radioimmunoassay every eight hours for four days in seven healthy control subjects and ten patients with hepatic disease (five with cirrhosis, three with primary biliary cirrhosis and ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.2.196
更新日期:1980-02-01 00:00:00
abstract::With the new screening and treatment guidelines and the prospect of human papillomavirus vaccination for adolescents, the current total volume of Papanicolaou (Pap) tests will be significantly reduced. We used available data to assess the current supply and demand in the cytotechnology labor market and how an expected...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/40DW4TG5TKNUEM71
更新日期:2007-10-01 00:00:00
abstract::The 1973 Comprehensive Blood Bank Survey Program of the College of American Pathologists was administered to 2,200 laboratories and blood banks throughout the United States. The results showed a considerable increase in the accuracy of the testing and in the performance of reagents. Accuracy of ABO testing was 99.3%; ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::A patient who had masses in the breasts clinically suggestive of neoplasia had fibrous mastopathy and striking deposition of ochronotic pigment within the soft tissue of the breast. The gross appearance and light microscopic features are illustrated. The histochemical features of ochronotic pigment and the difficulty ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/71.3.349
更新日期:1979-03-01 00:00:00
abstract::Acute paraplegia developed in a 53-year-old man with pulmonary aspergilloma because of contiguous extension of Aspergillus infection to the epidural and subdural spaces and spinal cord. Histopathologic findings of the spinal cord showed Aspergillus hyphae penetrating the myelin sheath and myelomalacia, predominantly i...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.6.763
更新日期:1985-12-01 00:00:00
abstract:OBJECTIVES:Preanalytical factors, such as hemolysis, affect many components of a test panel. Machine learning can be used to recognize these patterns, alerting clinicians and laboratories to potentially erroneous results. In particular, machine learning might identify which cases of elevated potassium from a point-of-c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa039
更新日期:2020-07-07 00:00:00
abstract::There are few studies that examine prevalence, quantity, and cellular proclivity of latent human herpesvirus 6 (HHV-6) in healthy populations. We examined 69 tonsils with paired blood specimens from children without evidence of acute infection. By polymerase chain reaction (PCR), HHV-6 was detected at low levels in 10...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/Y2HH-B1CK-0F5L-U7B8
更新日期:2001-11-01 00:00:00
abstract::The blood counts and lactic dehydrogenase values of eight patients with thrombotic thrombocytopenic purpura (TTP) were reviewed in relation to the clinical course. Three of the eight patients died. In these patients, the hemoglobin was significantly lower and the LDH higher at the time of presentation than that of the...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.5.700
更新日期:1983-11-01 00:00:00
abstract::Twenty cases of inflammatory myofibroblastic tumor (IMT) were studied; 19 involved the lung and 1 the esophagus only. The patients' ages ranged from 3 to 72 years. There were 9 males and 11 females. Involvement of a bronchus was seen in one case and of mediastinal structures in four. Chest pain and dyspnea were common...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.5.538
更新日期:1990-11-01 00:00:00
abstract::The authors investigated assays for free protein S (ProS) antigen, total ProS antigen, and ProS crossed immunoelectrophoresis (CIEP) in the diagnosis of type 1 inherited ProS deficiency. Accurate measurement of the hemostatically important free ProS required showing that, on each specimen, precipitation of the C4b-bin...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.2.176
更新日期:1990-08-01 00:00:00
abstract::Human papillomaviruses (HPVs) are associated with benign and malignant neoplasms of the cervix. One of the criteria for their etiologic role requires an assessment of whether virtually all or only a small fraction of lesions contain viral genomes. DNA preparations from colposcopically directed punch biopsies of cervic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.5.638
更新日期:1991-05-01 00:00:00
abstract::A 61-year-old man with acquired immunodeficiency syndrome (AIDS) sought care because of the onset of progressive dysphagia. He was found to have a perforated, fungating esophageal mass. The combined histologic and immunologic findings were diagnostic of Hodgkin's disease, nodular sclerosis type, lymphocyte-depleted va...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/108.5.593
更新日期:1997-11-01 00:00:00
abstract::The ideal method of heparinization should achieve therapeutic concentrations (0.2-0.5 IU/mL) in the artificial kidney and the least possible amount of heparin in the patient. Total heparinization using a bolus dose (8400 IU) followed by continuous infusion of heparin (20 IU/min), initially showed 1.4-2.4 IU/mL in the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.3.317
更新日期:1985-09-01 00:00:00
abstract::The significance of the human immunodeficiency virus (HIV) in the small intestinal lamina propria in patients with the acquired immune deficiency syndrome or conditions related to that syndrome who have chronic diarrhea and malabsorption is unclear. To investigate this issue, upper endoscopy (after a 12- to 16-hour fa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.1.21
更新日期:1992-01-01 00:00:00
abstract::Recent studies in Western populations have shown that trisomy 3 is the most frequent chromosomal abnormality in primary gastric lymphoma (PGL). To study the incidence of trisomy 3 and its implications for the pathogenesis of PGL in Hong Kong, we have applied the technique of chromosome in situ hybridization in 13 case...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/110.3.347
更新日期:1998-09-01 00:00:00
abstract::Kikuchi's lymphadenitis is a self-limiting condition typically affecting young patients. Surgical biopsy is unnecessary if a firm diagnosis can be rendered by fine-needle aspiration cytology (FNAC). The authors report the FNAC findings for 27 cases, including 24 female and 3 male patients aged 12 to 43 years. Histolog...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.4.454
更新日期:1994-10-01 00:00:00
abstract::Clotting tests based upon the activated partial thromboplastin time are disturbed by heparin. This brief communication shows that Factor XII clotting activity in plasma samples heparinized in vitro and in vivo can be measured accurately by means of an aPTT-test using congenitally deficient substrate plasma when the pl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.4.474
更新日期:1983-10-01 00:00:00
abstract:OBJECTIVES:Myeloid and lymphoid neoplasms with abnormalities of fibroblast growth factor receptor 1 gene (FGFR1) are a rare and aggressive disease group that harbors translocations of FGFR1 with at least 14 recognized partner genes. We report a case of a patient with a novel t(17;21)(p13;q22) with RUNX1 rearrangement a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCPUD6W1JLQQMNA
更新日期:2015-05-01 00:00:00
abstract::Twenty-five cases of tubal or tubo-endometrioid metaplasia that occurred in patients from 21 to 51 years of age (mean 39 years) are described. The majority of the cases were seen in consultation. They were referred because of diagnostic problems resulting from features that have received little emphasis. The metaplasi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.5.618
更新日期:1995-05-01 00:00:00